Dexamethasone (MK-125)

Name: Dexamethasone (MK-125)
Brand: Selleck Chemicals
SKU: S1322
Rating: 5 (50 reviews)

For research use only.

Catalog No.S1322 Synonyms: NSC 34521, Hexadecadrol, Prednisolone F

50 publications

Dexamethasone (MK-125) Chemical Structure

CAS No. 50-02-2

Dexamethasone (MK-125, NSC 34521, Hexadecadrol, Prednisolone F) is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects. Dexamethasone induces autophagy and mitophagy. Dexamethasone is tested in hospitalized patients with COVID-19 and is found to have benefits for critically ill patients.

Selleck's Dexamethasone (MK-125) has been cited by 50 publications

Cancer Discov, 2019, 9(5):628-645

PubMed: 30787016 ( click the link to review the publication )

Nat Med, 2017, 23(4):424-428

PubMed: 28263313 ( click the link to review the publication )

Cell Rep, 2021, 36(2):109360

PubMed: 34260922 ( click the link to review the publication )

Mol Cancer, 2021, 20(1):84

PubMed: 34090465 ( click the link to review the publication )

Oncogene, 2021, 1-11

PubMed: 34175886 ( click the link to review the publication )

Mol Cancer Ther, 2021, 20(2):367-378

PubMed: 33298585 ( click the link to review the publication )

Mol Cancer Res, 2021, molcanres.0808.2020

PubMed: 33980612 ( click the link to review the publication )

J Cell Mol Med, 2021, 10.1111/jcmm.16074

PubMed: 33491272 ( click the link to review the publication )

Food Funct, 2021, 10.1039/d0fo02387f

PubMed: 33432947 ( click the link to review the publication )

Blood Adv, 2021, 5(9):2391-2402

PubMed: 33950175 ( click the link to review the publication )

Proc Natl Acad Sci U S A, 2020, 202021450

PubMed: 33310900 ( click the link to review the publication )

Clin Cancer Res, 2020, 10.1158/1078-0432.CCR-20-1315

PubMed: 32826328 ( click the link to review the publication )

Blood Cancer J, 2020, 10(6):72

PubMed: 32591499 ( click the link to review the publication )

Int J Biol Sci, 2020, 16(4):543-552

PubMed: 32025204 ( click the link to review the publication )

Neurotox Res, 2020, 10.1007/s12640-020-00294-x

PubMed: 33025360 ( click the link to review the publication )

Drug Test Anal, 2020, 10.1002/dta.2917

PubMed: 32852861 ( click the link to review the publication )

Leuk Lymphoma, 2020, 1-10

PubMed: 32558607 ( click the link to review the publication )

Mol Med Rep, 2020, 22(4):2791-2800

PubMed: 32945456 ( click the link to review the publication )

Drug Metab Dispos, 2020, 48(8):613-621

PubMed: 32474442 ( click the link to review the publication )

Int J Cancer, 2020, 10.1002/ijc.33202

PubMed: 32638373 ( click the link to review the publication )

Int J Biol Sci, 2020, 16(13):2382-2391

PubMed: 32760206 ( click the link to review the publication )

Cancers (Basel), 2020, 12(8):E2092

PubMed: 32731472 ( click the link to review the publication )

BMC Cancer, 2020, 20(1):269

PubMed: 32228485 ( click the link to review the publication )

Adv Ther (Weinh), 2020, 2000034

PubMed: 32838027 ( click the link to review the publication )
Clin Cancer Res, 2019, 10.1158/1078-0432

PubMed: 31439587 ( click the link to review the publication )

Mol Ther, 2019, 10.1016/j.ymthe.2019.08.011

PubMed: 31501033 ( click the link to review the publication )

Free Radic Biol Med, 2019, 137:1-12

PubMed: 31004750 ( click the link to review the publication )

Int J Mol Sci, 2019, 20(24)E6175

PubMed: 31817853 ( click the link to review the publication )

Sci Rep, 2019, 9(1):1265

PubMed: 30718665 ( click the link to review the publication )

J Endod, 2019, 45(6):706-715

PubMed: 31056297 ( click the link to review the publication )

Behav Brain Res, 2019, 356:8-17

PubMed: 30092249 ( click the link to review the publication )

Evid Based Complement Alternat Med, 2019, 2019:2969364

PubMed: 31871475 ( click the link to review the publication )

Clin Cancer Res, 2018, 24(4):927-938

PubMed: 29158269 ( click the link to review the publication )

Stem Cell Res Ther, 2018, 9(1):58

PubMed: 29523187 ( click the link to review the publication )

Am J Physiol Cell Physiol, 2018, 315(3):C300-C309

PubMed: 29768047 ( click the link to review the publication )

Int J Oncol, 2018, 10.3892/ijo.2018.4635

PubMed: 30431078 ( click the link to review the publication )

Drug Des Devel Ther, 2018, 12:2361-2369

PubMed: 30122894 ( click the link to review the publication )

Mol Med Rep, 2018, 17(4):5887-5893

PubMed: 29436611 ( click the link to review the publication )

Oncol Lett, 2018, 15(5):6107-6114

PubMed: 29731842 ( click the link to review the publication )

Leukemia, 2017, 31(12):2652-2660

PubMed: 28479592 ( click the link to review the publication )

Oncogene, 2017, 36(40):5631-5638

PubMed: 28581522 ( click the link to review the publication )

Cell Physiol Biochem, 2017, 43(4):1547-1561

PubMed: 29035884 ( click the link to review the publication )

Mol Cancer Ther, 2017, 16(11):2586-2597

PubMed: 28835384 ( click the link to review the publication )

Toxicol Sci, 2017, 155(1):85-100

PubMed: 27664422 ( click the link to review the publication )

J Biol Chem, 2017, 292(9):3683-3691

PubMed: 28087699 ( click the link to review the publication )

Xenobiotica, 2016, 46(10):948-52

PubMed: 26822676 ( click the link to review the publication )

Nat Commun, 2015, 6:8481

PubMed: 26412466 ( click the link to review the publication )

Oncotarget, 2015, 6(38):40704-18

PubMed: 26513019 ( click the link to review the publication )
Assay Drug Dev Technol, 2014, 12(9-10):514-26

PubMed: 25506801 ( click the link to review the publication )

Oncogene, 2013, 32(10):1316-29

PubMed: 22543582 ( click the link to review the publication )

Purity & Quality Control

Choose Selective IL Receptor Inhibitors

Biological Activity

Description

Targets

interleukin receptor ^[1]
(Cell-free assay)

In vitro

Dexamethasone results in decrease in transmonolayer paracellular permeability mainly to sucrose, fluorescein and dextrans of up to 20 KDa in an immortalised rat brain endothelial cell line (GPNT). Dexamethasone results in filamentous actin and the cytoskeleton associated protein cortactin being highly concentrated in the regions of cell-cell contact with few F-actin stress fibres visible within the cytoplasm in cultured rat brain endothelial cells, an observation consistent with a more differentiated barrier phenotype induced by dexamethasone. Dexamethasone treatment has been shown to strongly stimulate the level of the Id-1 protein, which is a serum-inducible helix-loop-helix transcriptional repressor, involved in cell differentiation, and this effect was shown to be associated with reorganisation of ZO-1 to the cell periphery in Con8 mammary epithelial tumor cells. Dexamethasone prevents cytokine-induced enhanced expression of MMP-9 and alterations in the expression of ZO-1 in untreated GPNT monolayers. ^[1] Dexamethasone depletes both basal and TNF-alpha-stimulated GSH levels by down-regulating the gamma-GCS-heavy subunit transcription via a mechanism involving AP-1 (c-Jun) in alveolar epithelial cells. Dexamethasone decreases both basal and stimulated GSH levels (TNF-α-treated) in alveolar epithelial cells (A549), without any change in GSSG. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
human monocytes	NVP2T2oxTnWwY4Tpc44h[XO\|YYm=	NW\JfI9jPSEQvF2=	M{HXcFIxKGh?	NXq1RXdkSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCqdX3hckBud26xY4n0[ZMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWFNvaX7keYNm\CCFT2iyJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCyNEBpenNidYPpcochemGkYnn0JJBwdHmlbH;uZYwh[W62aXLv[JkhUWeJIHL5JHNFWy2SQVfFJIFv[Wy7c3nz	NEPjXIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi0N\|k{PSd-MkG4OFM6OzV:L3G+
rat polymorphonuclear leukocytes	MUnGeY5kfGmxbjDhd5NigQ>?		MmXvNlAhdWmwcx?=	MXTBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJJJifCCyb3z5cY9zeGixboXjcIVieiCuZYXrc4N6fGW\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhdmm2cnnjJI95cWSnIIDyc4R2[3Srb36gZYZ1\XJiMkCgcYlveyCkeTDHdolme3NibXX0bI9lNCCLQ{WwQVIvOyCwTR?=	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVyNk[yNEc,OjJ3ME[2NlA9N2F-
mouse thymocytes	NFLj[W9HfW6ldHnvckBie3OjeR?=	M{LheVEh\|ryP	NYjE[2I4OjRiaB?=	MmLtTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDtc5V{\SC2aIntc4N6fGW\|IHHzd4V{e2WmIHHzJIlvfGW{boXjcIVwe2:vYXygSG5CKG[{YXft[Y51[XSrb36gZZQhOSC3TTDh[pRmeiB{NDDodpMh[nliYXfhdo9{\SCpZXyg[Yxm[3S{b4Doc5Jme2m\|	NUHvSW1rRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PVQ4PDhpPkK0O\|k1PzR6PD;hQi=>
T-cell	M360dmZ2dmO2aX;uJIF{e2G7			MW\Jcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCWIHPlcIwheHKxbHnm[ZJifGmxbjD1d4lv\yClb37jZY5ifmGuaX6gRUB{fGmvdXzheIVlKG2rY3Wgd5Bt\WWwIHPlcIx{NCCLQ{WwJF0hOC5yMEWg{txONg>?	MlnuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{Mkm3OlcoRjFzMkK5O\|Y4RC:jPh?=
PBMC	NGrpe3FHfW6ldHnvckBie3OjeR?=			MVvJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCWTl[tZYxxcGFicILv[JVkfGmxbjDmdo9uKGi3bXHuJJBmemmyaHXyZYwh[myxb3SgcY9vd263Y3zlZZIh[2WubIOgd5RqdXWuYYTl[EBjgSCOUGOsJGlEPTBiPTCwMlAyKM7:TT6=	M1TiSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMkK5O\|Y4Lz5zMUKyPVc3PzxxYU6=
PBMC	MUfGeY5kfGmxbjDhd5NigQ>?			NV\6R3Y{UW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4Sg[5Jwf3SqIH;mJGxRWyC\|dHnteYxifGWmIHj1cYFvKHCncnnwbIVz[WxiYnzvc4QhdW:wb371Z4xm[XJiY3XscJMtKEmFNUCgQUAxNjB2IN88UU4>	NInZOok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUKyPVc3Pyd-MUGyNlk4Pjd:L3G+
RAW264.7	NHqxeFBCdnSraX7mcIFudWG2b4L5JIF{e2G7		MU[yOEBpenN?	NXqxZnhpSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCOUGOtd5RqdXWuYYTl[EBud3W\|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDl[oZm[3Rib36gWG5H[WyyaHGg[o9zdWG2aX;uJJBz\XS{ZXH0[YQhOSCqcjDi[YZwemViTGDTJINp[WyuZX7n[UBu\WG\|dYLl[EBi\nSncjCyOEBpenNuIFnDOVAhRSByLkeg{txONg>?	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTlyOEm4OEc,OTF7MEi5PFQ9N2F-
N9	MXvGeY5kfGmxbjDhd5NigQ>?			NV65Z5J{UW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KFSQRj3hcJBp[SC{ZXzlZZNmKGmwIF65JINmdGy\|IHnuJJRp\SC\|dYDldo5ifGGwdDygTWM2OCB;IECuNFc1KM7:TT6=	NUf6Vm1QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKzOlE{QTVpPkGyN\|YyOzl3PD;hQi=>
RAW 264.7	NV:2[GlmTnWwY4Tpc44h[XO\|YYm=			NFvVb5hKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhXE6ILXHsdIhiKHKnbHXhd4UhcW5iUlHXJFI3PC55IHPlcIx{KGmwIITo[UB{fXCncn7heIFvfCxiSVO1NEA:KDBwNEKg{txONg>?	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjN4MUO5OUc,OTJ\|NkGzPVU9N2F-
A549	M4joXWZ2dmO2aX;uJIF{e2G7	MUmxNEB2VQ>?		M1vWXWFvfGGpb37pd5Qh[WO2aY\peJkh\m:{IFfseYNw[2:{dHnjc4llKHKnY3XweI9zKGmwIF3NWHYhfHKjboPhZ5RqfmG2aX;uJIF{e2G7IHnuJIh2dWGwIFG1OFkhdHWwZzDldIl1cGWuaXHsJINmdGy\|IHH0JFExKHWPLDDJR\|UxKD1iMUCg{txONg>?	NXzGN2lVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW5PVk6QDlpPkG1PVk6QTh7PD;hQi=>
A549	NEHTSZlHfW6ldHnvckBie3OjeR?=			NHW5NnZC[3Srdnn0fUBifCCpbIXjc4NwenSrY3;p[EBz\WOncITvdkBqdiCqdX3hckBCPTR7IHPlcIx{KGG\|c3Xzd4VlKGG\|IHXm[oVkfCCxbjDHVmUheHKxbX;0[ZIhemW\|cH;ud4Uh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDhd5NigSxiRVO1NEA:KDBwMECxJO69VS5?	NF:1W3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNk[0N\|A3OCd-MU[2OFMxPjB:L3G+
A549	NUjoT5diTnWwY4Tpc44h[XO\|YYm=			NInlNHVC\2:waYP0JIFkfGm4aYT5JIF1KEeUIHHzd4V{e2WmIHHzJG1OXFZvbXXkbYF1\WRidILhcpNi[3SrdnH0bY9vKG:oIILlcoltdGFibIXjbYZmemG\|ZTDn[Y5mKGmwIHj1cYFvKEF3NEmgZ4VtdHNicnXsZZRqfmVidH:gSIV5[W2ndHjhd49v\SxiRVO1NEA:KDBwMEC1NFEzKM7:TT6=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjh{MUe4NUc,OTZ6MkG3PFE9N2F-
A549	MXXGeY5kfGmxbjDhd5NigQ>?			NX3qXYc3SWexbnnzeEBi[3Srdnn0fUBifCCJUjDhd5Nme3OnZDDhd{BPTi2tYYDwZWIudWWmaXH0[YQhfHKjboPy[ZBz\XO\|aX;uJI9nKHOnY4LleIVlKHCuYXPlcpRidCCjbHvhcIlv\SCyaH;zdIhifGG\|ZTDn[Y5mKGmwIHj1cYFvKEF3NEmgZ4VtdHNuIFnDOVAhRSByLkCwNVE4PSEQvF2u	NUDqU5F[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[4NlE4QDFpPkG2PFIyPzhzPD;hQi=>
HFF	NILCVFdHfW6ldHnvckBie3OjeR?=			NEnDcJFKdmS3Y4Tpc44hd2ZiYYLvcYF1[XOnIHHjeIl3cXS7IHnuJGhHTiClZXzsd{Bie3Onc4Pl[EBieyCycn;keYN1cW:wIH;mJIJmfGFiZYP0doFlcW:uIHL5JIFzd22jdHHz[UBie3OjeTygSWM2OCB;IECuNFAyQSEQvF2u	M{nYVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUixNVczLz5zN{G4NVE4OjxxYU6=
HeLa	MWHGeY5kfGmxbjDhd5NigQ>?			M{POd2FkfGm4YYTpc44hd2ZiTV3UWkBqdiCKZVzhJINmdGy\|IH3lZZN2emWmIHL5JIx2[2moZYLhd4Uh[WO2aY\peJktKEWFNUCgQUAxNjBzNzFOwG0v	MlvCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzOEGxO\|IoRjF5MUixNVczRC:jPh?=
HFF	MWjGeY5kfGmxbjDhd5NigQ>?			MnnGTY5pcWKrdHnvckBw\iCLTEGtd5RqdXWuYYTl[EBKVDZicILv[JVkfGmxbjDpckBJTkZiY3XscJMtKEmFNUCgQUAxNjByMEWxJO69VS5?	Mli4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzOEGxO\|IoRjF5MUixNVczRC:jPh?=
RAW 264.7	M4e2[mZ2dmO2aX;uJIF{e2G7		M165T\|I1KGi{cx?=	MknITY5pcWKrdHnvckBw\iCrboTldoZmem:wIHfhcY1iNXO2aX31cIF1\WRiTl:gdJJw\HWldHnvckBqdiCUQWegNlY1NjdiY3XscJMh[W[2ZYKgNlQhcHK\|LDDJR\|UxKD1iMD6wNkDPxE1w	M2m2O\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{[3NVI1Lz5zN{O2O\|EzPDxxYU6=
RAW264.7	MkHRSpVv[3Srb36gZZN{[Xl?		M3q3TVIhcHK\|	MWTJcohq[mm2aX;uJI9nKEORWEKg[ZhxemW\|c3nvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W\|ZTDSRXczPjRwNzDj[YxteyCjZoTldkAzKGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	M{fSelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEC3Nlc4Lz5zN{SwO\|I4PzxxYU6=
RAW264.7	MUfGeY5kfGmxbjDhd5NigQ>?		NHnqT\|UzKGi{cx?=	Mnu2TY5pcWKrdHnvckBw\iCvUFfFV\|Eh\XiycnXzd4lwdiCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDh[pRmeiB{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	NF2ydoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SwO\|I4Pyd-MUe0NFczPzd:L3G+
A549	MV\GeY5kfGmxbjDhd5NigQ>?			MnnhRYdwdmm\|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDBOVQ6KGOnbHzzJIJ6KE2PVG[geJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMEWzO{DPxE1w	NHu0SGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[xOlM6PSd-MUe2NVY{QTV:L3G+
A549	Mn3nSpVv[3Srb36gZZN{[Xl?			M2TzT2Fod26rc4SgZYN1cX[rdImgZZQh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KgbY4hcHWvYX6gRVU1QSClZXzsd{BjgSCQRj3rZZBx[UJidILhcpNz\XC{ZYPzbY9vKGG\|c3H5MEBKSzVyIE2gNE4xODFizszNMi=>	M4fmcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkG2N\|k2Lz5zN{[xOlM6PTxxYU6=
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... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Bcl-2 / Bcl-xl / Mcl-1 / Survivin / IAP1 ; PubMed: 28696369 Molecules Bcl-xl, Bcl-2, Survivin, Mcl-1, and IAP1 protein levels were determined via Western blot analysis. Cyclin D1 / Cyclin E / COX-2 / MMP-9 ; PubMed: 28696369 Molecules Cyclin D1, Cyclin E, COX-2, and MMP-9 protein levels were determined via Western blot analysis. Bax / p21; PubMed: 28696369 Molecules The protein levels of Bax and p21 were determined via Western blot analysis. p-JNK / JNK / p-p38 / p38 / p-ERK / ERK ; PubMed: 28696369 Molecules The cells were treated with 100 μM of DHAP for the time periods stated; whole-cell extracts were then prepared and analyzed via Western blot analysis for p-p38 (Thr180/Tyr182), p-JNK (Thr183/Tyr185), and p-ERK1/2 (Thr202/Tyr204) by Western blot analysis. To confirm equal protein loading, the immunoblot was stripped and reprobed for JNK, p38, and ERK1/2. The results shown are representative of the three independent experiments	28696369

In vivo

Dexamethasone is administered i.m. to pregnant ewes, leads to the following results (1) blood pressure is unchanged; (2) as previously reported in the fetus, sensitivity to endothelin-1 (ET) is increased; (3) acetylcholine-induced relaxation is increased; (4) L-NAME suppressible vasodilatory response to ET is abolished; (5) there is no change in endothelium-independent vasodilatation; and (6) there is no change in eNOS RNA and protein levels, when compared to saline treated controls. ^[3]

Protocol

References

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Solubility (25°C)

In vitro	DMSO	79 mg/mL (201.29 mM)
	Water	Insoluble
	Ethanol	'''6 mg/mL
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+45% PEG 300+H2O For best results, use promptly after mixing.	13mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Dexamethasone (MK-125) SDF

Molecular Weight	392.46
Formula	C₂₂H₂₉FO₅
CAS No.	50-02-2
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	NSC 34521, Hexadecadrol, Prednisolone F
Smiles	CC1CC2C3CCC4=CC(=O)C=CC4(C3(C(CC2(C1(C(=O)CO)O)C)O)F)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

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Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04911361	Not yet recruiting	Drug: Dexamethasone	Dry Eye	Research Insight LLC	July 26 2021	Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
I'm loonkig for Dexamethasone to use in cell culture, would it be better to use the Dexamethasone S1322, the sodiume phosphate S4028 or the acetate S3124?
Answer:
S1322 is the free base form of Dexamethasone, S4028 is the sodium phosphate form and S3124 is the acetate salt. All of them have similar biological activity in tissue culture, however, their suitabilities vary in different solvents. As free base, S1322 has high solubility in DMSO (79mg/ml), poor solubility in water (<1mg/ml), and moderate solubility in ethanol (6mg/ml). As sodium phosphate salt, S4028 has high solubility in water (103mg/ml) but is insoluble in DMSO and ethanol (<1mg/ml). The solubility of S3124 is 87mg/ml in DMSO, <1mg/ml in water, and 20mg/ml in ethanol. Selection can be made based on the customer's experimental setup.

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Dexamethasone (MK-125)