Dexamethasone (DHAP)
Catalog No.S1322
Molecular Weight(MW): 392.46
Dexamethasone (DHAP) is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects.
Cited by 2 Publications
2 Customer Reviews
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Dexamethasone and largazole cooperate to suppress invasion and to restore E-cadherin localization to the cell peripher y. ( a) Phase contrast micrographs showing morphological changes in MDA-MB-231 cells induced by E-cadherin expression combined with 100 nM dexamethasone and 10 nM largazole treatments. Insets show the cells at higher magnification. (b ) Fluorescence (E-Cad-GFP) or immunofluorescence microscopy (g -catenin (g-Cat.)) of 231/E-Cad-GFP cells treated for 72 h with vehicle (Control), 100 n M dexamethasone, 10 nM largazole or 100 nM dexamethasone + 10 nM largazole (Dex. + Larg.). (c ) Invasion assays were per formed with the indicated cell lines treated for 72 h with or without 100 nM dexamethasone + 10 nM largazole using modified Boyden chambers impregnated with matrigel. The results are presented as the average number of cells that invaded through the membrane per field s.d. of five randomly chosen fields, and are representative of three independently per formed experiments.
Oncogene 2013 32, 1316-29. Dexamethasone (DHAP) purchased from Selleck.
(d) BT549 cells were treated and analyzed by immunofluorescence microscopy as in Figure b. (e) BT549 cells were treated as described in Figure b and analyzed for invasion as in Figure 3c. (f) Quantitation of junctional E-cadherin staining of the indicated cell lines treated with DMSO vehicle or Dex.+ Larg. as described in Figure b. Results are presented as the mean of analyses of three different fields of cells for each sample±s.d. Statistical significance was assessed using Student’s t -test.
Oncogene 2013 32, 1316-29. Dexamethasone (DHAP) purchased from Selleck.
Purity & Quality Control
Choose Selective IL Receptor Inhibitors
Biological Activity
| Description | Dexamethasone (DHAP) is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| In vitro |
Dexamethasone results in decrease in transmonolayer paracellular permeability mainly to sucrose, fluorescein and dextrans of up to 20 KDa in an immortalised rat brain endothelial cell line (GPNT). Dexamethasone results in filamentous actin and the cytoskeleton associated protein cortactin being highly concentrated in the regions of cell-cell contact with few F-actin stress fibres visible within the cytoplasm in cultured rat brain endothelial cells, an observation consistent with a more differentiated barrier phenotype induced by dexamethasone. Dexamethasone treatment has been shown to strongly stimulate the level of the Id-1 protein, which is a serum-inducible helix-loop-helix transcriptional repressor, involved in cell differentiation, and this effect was shown to be associated with reorganisation of ZO-1 to the cell periphery in Con8 mammary epithelial tumor cells. Dexamethasone prevents cytokine-induced enhanced expression of MMP-9 and alterations in the expression of ZO-1 in untreated GPNT monolayers. [1] Dexamethasone depletes both basal and TNF-alpha-stimulated GSH levels by down-regulating the gamma-GCS-heavy subunit transcription via a mechanism involving AP-1 (c-Jun) in alveolar epithelial cells. Dexamethasone decreases both basal and stimulated GSH levels (TNF-α-treated) in alveolar epithelial cells (A549), without any change in GSSG. [2] |
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| Cell Data |
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| In vivo | Dexamethasone is administered i.m. to pregnant ewes, leads to the following results (1) blood pressure is unchanged; (2) as previously reported in the fetus, sensitivity to endothelin-1 (ET) is increased; (3) acetylcholine-induced relaxation is increased; (4) L-NAME suppressible vasodilatory response to ET is abolished; (5) there is no change in endothelium-independent vasodilatation; and (6) there is no change in eNOS RNA and protein levels, when compared to saline treated controls. [3] |
Protocol
Solubility (25°C)
| In vitro | DMSO | 79 mg/mL (201.29 mM) |
|---|---|---|
| Ethanol | 6 mg/mL (15.28 mM) | |
| Water | Insoluble | |
| In vivo | Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+45% PEG 300+H2O For best results, use promptly after mixing. |
13mg/mL |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 392.46 |
|---|---|
| Formula | C22H29FO5 |
| CAS No. | 50-02-2 |
| Storage | powder |
| in solvent | |
| Synonyms | N/A |
Bio Calculators
Molarity Calculator
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Dilution Calculator
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Molarity Calculator
Clinical Trial Information
| NCT Number | Recruitment | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|
| NCT03472573 | Recruiting | Recurrent B Acute Lymphoblastic Leukemia|Refractory B Acute Lymphoblastic Leukemia | Sidney Kimmel Cancer Center at Thomas Jefferson University|Pfizer|Thomas Jefferson University | May 9 2018 | Phase 1 |
| NCT03367572 | Recruiting | Breast Carcinoma | Gary Morrow|National Cancer Institute (NCI)|University of Rochester | March 9 2018 | Phase 3 |
| NCT03374514 | Recruiting | Sensorineural Hearing Loss|Cochlear Implant | Hospital Italiano de Buenos Aires | August 9 2017 | Phase 3 |
| NCT03136146 | Recruiting | Hematopoietic/Lymphoid Cancer|Acute Lymphoblastic Leukemia|Lymphoblastic Lymphoma|Burkitt Leukemia/Lymphoma | M.D. Anderson Cancer Center | August 9 2017 | Phase 2 |
| NCT02477215 | Active not recruiting | Multiple Myeloma | Parameswaran Hari|Medical College of Wisconsin | October 9 2015 | Phase 1|Phase 2 |
| NCT02412878 | Active not recruiting | Multiple Myeloma | Amgen | September 9 2015 | Phase 3 |
Tech Support
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.
Frequently Asked Questions
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Question 1:
I'm loonkig for Dexamethasone to use in cell culture, would it be better to use the Dexamethasone S1322, the sodiume phosphate S4028 or the acetate S3124?
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Answer:
S1322 is the free base form of Dexamethasone, S4028 is the sodium phosphate form and S3124 is the acetate salt. All of them have similar biological activity in tissue culture, however, their suitabilities vary in different solvents. As free base, S1322 has high solubility in DMSO (79mg/ml), poor solubility in water (<1mg/ml), and moderate solubility in ethanol (6mg/ml). As sodium phosphate salt, S4028 has high solubility in water (103mg/ml) but is insoluble in DMSO and ethanol (<1mg/ml). The solubility of S3124 is 87mg/ml in DMSO, <1mg/ml in water, and 20mg/ml in ethanol. Selection can be made based on the customer's experimental setup.
