Dexamethasone

Catalog No.S1322

Dexamethasone  Chemical Structure

Molecular Weight(MW): 392.46

Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects.

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In DMSO USD 130 In stock
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Cited by 12 Publications

3 Customer Reviews

  • Dexamethasone and largazole cooperate to suppress invasion and to restore E-cadherin localization to the cell peripher y. ( a) Phase contrast micrographs showing morphological changes in MDA-MB-231 cells induced by E-cadherin expression combined with 100 nM dexamethasone and 10 nM largazole treatments. Insets show the cells at higher magnification. (b ) Fluorescence (E-Cad-GFP) or immunofluorescence microscopy (g -catenin (g-Cat.)) of 231/E-Cad-GFP cells treated for 72 h with vehicle (Control), 100 n M dexamethasone, 10 nM largazole or 100 nM dexamethasone + 10 nM largazole (Dex. + Larg.). (c ) Invasion assays were per formed with the indicated cell lines treated for 72 h with or without 100 nM dexamethasone + 10 nM largazole using modified Boyden chambers impregnated with matrigel. The results are presented as the average number of cells that invaded through the membrane per field s.d. of five randomly chosen fields, and are representative of three independently per formed experiments.

    Oncogene 2013 32, 1316-29. Dexamethasone purchased from Selleck.

    (d) BT549 cells were treated and analyzed by immunofluorescence microscopy as in Figure b. (e) BT549 cells were treated as described in Figure b and analyzed for invasion as in Figure 3c. (f) Quantitation of junctional E-cadherin staining of the indicated cell lines treated with DMSO vehicle or Dex.+ Larg. as described in Figure b. Results are presented as the mean of analyses of three different fields of cells for each sample±s.d. Statistical significance was assessed using Student’s t -test.

    Oncogene 2013 32, 1316-29. Dexamethasone purchased from Selleck.

  • Effect of SENP2 overexpression on apoptosis-related proteins in CLL cells with or without dexamethasone treatment. (A and B) MEC2 cells in which SENP2 was overexpressed and the control cells were cultured in 6 cm dishes and then treated with dexamethasone for 24 h after reaching 70% confluence. Western blot analysis was then performed to inspect the expression levels of cleaved caspase-9, caspase-9, cleaved caspase-3, caspase-3, c-Myc, p53, Bax and Bcl-2 in these cells. GAPDH was used to confirm equal amount of proteins loaded in each lane. CLL, chronic lymphocytic leukemia; NC-OE-SENP2, MEC2 cells transfected with null (control) overexpression vector; OE-SENP2, MEC2 cells transfected with SENP2 overexpression vector; NC-shRNA-SENP2, MEC2 cells transfected with null control shRNA; shRNA-SENP2, MEC2 cells transfected with shRNA against SENP2; +, dexamethasone treatment, −, no dexamethasone treatment.

    Int J Oncol, 2018, doi:10.3892/ijo.2018.4635. Dexamethasone purchased from Selleck.

Purity & Quality Control

Choose Selective IL Receptor Inhibitors

Biological Activity

Description Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects.
Targets
interleukin receptor [1]
(Cell-free assay)
In vitro

Dexamethasone results in decrease in transmonolayer paracellular permeability mainly to sucrose, fluorescein and dextrans of up to 20 KDa in an immortalised rat brain endothelial cell line (GPNT). Dexamethasone results in filamentous actin and the cytoskeleton associated protein cortactin being highly concentrated in the regions of cell-cell contact with few F-actin stress fibres visible within the cytoplasm in cultured rat brain endothelial cells, an observation consistent with a more differentiated barrier phenotype induced by dexamethasone. Dexamethasone treatment has been shown to strongly stimulate the level of the Id-1 protein, which is a serum-inducible helix-loop-helix transcriptional repressor, involved in cell differentiation, and this effect was shown to be associated with reorganisation of ZO-1 to the cell periphery in Con8 mammary epithelial tumor cells. Dexamethasone prevents cytokine-induced enhanced expression of MMP-9 and alterations in the expression of ZO-1 in untreated GPNT monolayers. [1] Dexamethasone depletes both basal and TNF-alpha-stimulated GSH levels by down-regulating the gamma-GCS-heavy subunit transcription via a mechanism involving AP-1 (c-Jun) in alveolar epithelial cells. Dexamethasone decreases both basal and stimulated GSH levels (TNF-α-treated) in alveolar epithelial cells (A549), without any change in GSSG. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CV1 cells MWrGeY5kfGmxbjDhd5NigQ>? NXP4VotESWO2aY\peJkh[XRiaIXtZY4h\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOYMTDj[YxteyCjc4Pld5Nm\CCjczDy[ZBz\XO|aX;uJI9nKFSQRjDhcoQhUUxvMT3i[ZRiNWmwZIXj[YQhTS2|ZXzlZ5RqdiCpZX7lJIV5eHKnc4Ppc44h[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGTDVyPUCuNUBvVQ>? NWG1cW1[OThyM{K2NVA>
HEK293 cells NH7LXVVHfW6ldHnvckBie3OjeR?= NEHCNpJFcXOybHHj[Y1mdnRib3[gX|NJZS2jbHTvd5Rmem:wZTDmdo9uKGi3bXHuJI1qdmW{YXzvZ49zfGmlb3nkJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOsJGtqRTBwM{[gcm0> NELlOmMzPDR2NkeyPC=>
human PBMC M3vsb2Z2dmO2aX;uJIF{e2G7 NVvZUJVFSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCqdX3hckBRSk2FIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYX70bU1ETDNvaX7keYNm\CCJTT3DV2YheHKxZIXjeIlwdiCjZoTldkA1QCCqcoOsJGVEPTB;MD60O{BvVQ>? NUS4bJU3OjNzOUm0PFU>
HFF cells NFO0NWVHfW6ldHnvckBie3OjeR?= M1nqTGFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDJUFEhe3SrbYXsZZRm\CCLTE[gdJJw\HWldHnvckBqdiCKRl[gZ4VtdHNiYomgeJJidnO{ZYDy[ZN{cW:wIHHzd4F6NCCHQ{WwQVAvPSCwTR?= NWD0eXNPOTd4OUK1NVk>
human A549 cells NFHtb2xHfW6ldHnvckBie3OjeR?= NXTSNZQ5SWO2aY\peJkh[XRiZ3z1Z49kd3K2aXPvbYQhemWlZYD0c5IhcW5iaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDl[oZm[3Rib36gS3JGKHC{b33veIVzKHKnc4DvcpNmKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[XluIFXDOVA:OSCwTR?= NFHkd4MyPjZ2M{C2NC=>
NHDFneo cells MmDESpVv[3Srb36gZZN{[Xl? MlTuRYN1cX[rdImgZZQhcHWvYX6gS3Ih\XiycnXzd4VlKGmwIF7ISGZv\W9iY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBKVC14IIDyc4R2[3Srb36sJGlEPTB;MT60JI5O M17pO|E4PzB3M{[y
human HeLa cells M1vkXGZ2dmO2aX;uJIF{e2G7 M1S1O3Rz[W6|cnXwdoV{e2mxbjDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcj3t[YRq[XSnZDDhcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDUVGEucW6mdXPl[EBkd2yuYXflcoF{\SCycn;tc5RmeiCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9NU42QCCwTR?= NH7PbnYzODd{N{e0Ny=>
human SW1353 cells M37GcmZ2dmO2aX;uJIF{e2G7 NFvpUVNVemGwc4LldJJme3Orb36gZYN1cX[rdImgZZQh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KgbY4hcHWvYX6gV3cyOzV|IHPlcIx{KGG|c3Xzd4VlKGG|IILldJJme3Orb36gc4YhUUxvMT3pcoR2[2WmIF3NVE0yOyCycn;keYN1cW:wIHHmeIVzKDJ2IHjyd{BjgSCHTFnTRUwhUUN3ME2xMlghdk1? M4HDfFI2PzB4MUCw
rat polymorphonuclear leukocytes MoHhSpVv[3Srb36gZZN{[Xl? NFXZWXMzOCCvaX7z MUPBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJJJifCCyb3z5cY9zeGixboXjcIVieiCuZYXrc4N6fGW|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhdmm2cnnjJI95cWSnIIDyc4R2[3Srb36gZYZ1\XJiMkCgcYlveyCkeTDHdolme3NibXX0bI9lNCCLQ{WwQVIvOyCwTR?= NHzyTHEzOjVyNk[yNC=>
human THP1 cells MVHGeY5kfGmxbjDhd5NigQ>? NF7PbZhVemGwc4LldJJme3Orb36gZYN1cX[rdImgZZQh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KtcYVlcWG2ZXSgZY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBpfW2jbjDUTHAyKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhUUxvODDwdo9lfWO2aX;uMEBKSzVyPUKuOVEhdk1? M3vPV|IxPzJ5N{Sz
human NP1 cells NGXRWGhHfW6ldHnvckBie3OjeR?= NEXpbW9C\2:waYP0JIFkfGm4aYT5JIF1KEeUIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iaIXtZY4hVlBzIHPlcIx{KGG|c3Xzd4VlKGG|IHfseYNw[2:{dHnjc4llKHKnc4DvcpNmKGWuZX3lcpQhfHKjboPhZ5RqfmG2aX;uJIJ6KEeDTESgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVQvOiCwTR?= MnvkNVk{OjF|NEG=
human HeLa cells M4DZXGZ2dmO2aX;uJIF{e2G7 MoXyWJJidnOjY4TpeoF1cW:wIH;mJGdTNUyERDDlfJBz\XO|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bkdy2neIDy[ZN{cW6pIFfBUFQuTEKGIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF01NjJibl2= M4fUfVI1ODFzNkS0
human 13D3/Huh7 cells M4fPdWZ2dmO2aX;uJIF{e2G7 NUS4blU{OjRiaB?= MmXXWJJidnOjY4TpeoF1cW:wIHHjeIl3cXS7IHH0JIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHnuJIh2dWGwIEGzSFMwUHWqNzDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhfHm{b4PpcoUh[W2rbn;0doFve2[ncnHz[UBi[3Srdnn0fUBi\nSncjCyOEBpenNuIFXDOVA:PSCwTR?= MmThNlExPzNzOUC=
rat R1 cells MoDtSpVv[3Srb36gZZN{[Xl? NEHzUGwyOCEQvF2= MWGyOEBp M4TxPGlvcGmkaYTpc44hd2ZiZ3z1Z49kd3K2aXPvbYQhemWlZYD0c5Iu\2y3Y3;jc5J1cWOxaXSgZ49ueGyneDD0doFve2[nY4Tl[EBqdiC{YYSgVlEh[2WubIOgZZN{\XO|ZXSgZZMh\G:5bj3y[Yd2dGG2aX;uJI9nKFSSQT3y[ZNxd26|aY\lJIVt\W2nboSgdJJwcW6obHHtcYF1d3K7IHflcoUh\XiycnXzd4lwdiCjdDCxNEB2VSCjZoTldkAzPCCqcoOgZpkh[mW2YT3nZYxi[3Sxc3nkZZNmKGG|c3H5MEBKSzVyPUWgcm0> M1fIVVE5PDF{M{m3
human RPMI8226 cells NUDrXJhsWHKxbHnm[ZJifGmxbjDhd5NigQ>? NITiTGs1KGSjeYO= MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKSTVm4NlI3KGOnbHzzJIFnfGW{IESg[IF6eyxiSVO1NF03NjVibl2= M{juNFE4PzB3M{[y
african green monkey COS7 cells NFr0eGNHfW6ldHnvckBie3OjeR?= MlTURYdwdmm|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHHmdolk[W5iZ4Ll[Y4hdW:wa3X5JGNQWzdiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCJYXy0MWxDTCCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9O{4zKG6P M3TmWlE6QDZ|MEiz
human PBMC NUDK[5FjTnWwY4Tpc44h[XO|YYm= M4PwWVI1KGh? M3T4ZmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hcHWvYX6gVGJOSyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFCKQT3pcoR2[2WmIFnGUodidW2jIIDyc4R2[3Srb36gZYZ1\XJiMkSgbJJ{ NXG1dlcyOjNzOUm0PFU>
human H13 cells MX\GeY5kfGmxbjDhd5NigQ>? MV;Jcohq[mm2aX;uJI9nKGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJGgyOyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIdtfWOxY3;yeIlkd2mmLXnu[JVk\WRiY3XscEBxem:uaX\ldoF1cW:wIHHmeIVzKDNiaILzJIJ6KFt|SG2teIh6dWmmaX7lJIlv[2:{cH;yZZRqd25iYYPzZZktKEWFNUC9PU44KG6P NIjzWFkzOzF7OUS4OS=>
mouse RAW264.7 cells NXPUW4g3TnWwY4Tpc44h[XO|YYm= M2PzeFE5KGh? M3nIUWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmYu[WyyaHGgd4VkemW2aX;uJIFnfGW{IEG4JIhzeyxiSVO1NF0xNjBzIN88US=> M2e5O|IyOzV|NUSz
293 MSR cells MorMSpVv[3Srb36gZZN{[Xl? MXm2NEBucW6| NEC4emNFcXOybHHj[Y1mdnRib3[gX|NJZS2mZYjhcYV1cGG|b37lJIh2dWGwIHfseYNw[2:{dHnjc4llKHKnY3XweI9zKGGucHjhJIV5eHKnc4Pl[EBqdiB{OUOgUXNTKGOnbHzzJIFnfGW{IE[wJI1qdnNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiRVO1NF0yOi53IH7N NVi4Sod1OjNzOUm0PFU>
Sf9 cells NHTXdVNHfW6ldHnvckBie3OjeR?= MUjCbY5lcW6pIHHm[olvcXS7IITvJIh2dWGwIILlZ49u[mmwYX70JIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCVZkmgZ4VtdHN? MX2yNFczPzd2Mx?=
RAW 264.7 cells MnjYSpVv[3Srb36gZZN{[Xl? M1jMbVI1KGh? M4XqS2lvcGmkaYTpc44hd2ZiaX70[ZJn\XKxbjDnZY1u[S2|dHnteYxifGWmIF7PJJBzd2S3Y4Tpc44hcW5iUlHXJFI3PC55IHPlcIx{KGGodHXyJFI1KGi{czygTWM2OD1yLkCyJO69VQ>? NFjMfXMyPzN4N{GyOC=>
mouse J774A.1 cells Mn7pSpVv[3Srb36gZZN{[Xl? NYTkdIdOUW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBVVk[jbIDoZUBxem:mdXP0bY9vKGmwIH3veZNmKEp5N{TBMlEh[2WubIOsJGlEPTB;NEigcm0> MX[xO|g6Ojl2MR?=
human HepG2 cells MUXGeY5kfGmxbjDhd5NigQ>? NHXYb3EzPCCq MkXHRYN1cX[jdHnvckBw\iC{YYSgVHhTKGW6cILld5Nm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGKjc3XkJIx2dWmwZYPj[Y51KGGwYXz5d4l{NCCHQ{WwQVIvPiEQvF2= M4GxOVIxQTZ4MESz
human U2OS cells NGflNVZHfW6ldHnvckBie3OjeR?= MX[xPEBp Mnm2RYdwdmm|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcj3MRmQhcW5iaIXtZY4hXTKRUzDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKEejbEStSGJFKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJJRz[W6|YXP0bZZifGmxbjDhZ5Rqfmm2eTDh[pRmeiBzODDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUG1Mlg1QDlizszN MYCyNFgyOjZ6MR?=
mouse thymocytes MWPGeY5kfGmxbjDhd5NigQ>? NEnuVlAyKM7:TR?= M164OlI1KGh? MnrkTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDtc5V{\SC2aIntc4N6fGW|IHHzd4V{e2WmIHHzJIlvfGW{boXjcIVwe2:vYXygSG5CKG[{YXft[Y51[XSrb36gZZQhOSC3TTDh[pRmeiB{NDDodpMh[nliYXfhdo9{\SCpZXyg[Yxm[3S{b4Doc5Jme2m| M{H1elI1Pzl2N{S4
human HMC1 cells NHixVIdHfW6ldHnvckBie3OjeR?= Mn;oNUBp MVXtcZVvd22xZIXsZZRwenliYXP0bZZqfHliaX6gbJVu[W5iSF3DNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIIDoc5Jjd2xiMUKtcZlzcXO2YYTlJFE{NWGlZYTheIUwSTJ|MUi3MZN1cW23bHH0[YQhUUxzLXLleIEhe2WlcnX0bY9vKHS{ZXH0[YQhOSCqcjDwdolweiC2bzCzNEBucW6|IIDoc5Jjd2xiMUKtcZlzcXO2YYTlJFE{NWGlZYTheIUwSTJ|MUi3JINp[WyuZX7n[UBjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{ M{nmb|IyOzB{OU[3
human monocytes MlrkSpVv[3Srb36gZZN{[Xl? NVjDN5R[PSEQvF2= NHnJN5AzOCCq MojERY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBpfW2jbjDtc45w[3m2ZYOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBEV1h{IHX4dJJme3Orb36gZZQhPSC3TTDh[pRmeiB{MDDodpMhfXOrbnegdoFj[mm2IIDvcJlkdG:wYXygZY51cWKxZImgTYdIKGK7IGPEV{1RSUeHIHHuZYx6e2m| MVmyNVg1Ozl|NR?=
COS7 cells MYnGeY5kfGmxbjDhd5NigQ>? NGLkbWsyKM7:TR?= Mm\KRYdwdmm|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDROVcxSSCvdYThcpQhcW5iQ1;TO{Bk\WyuczDheEAyKHWPIHL5JG5HNWujcIDhRk1tfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? MkewNVgxOzh7N{C=
human MDA-kb2 cells MWXGeY5kfGmxbjDhd5NigQ>? MorDRYdwdmm|dDDhZ5Rqfmm2eTDheEBidmS{b3flckBz\WOncITvdkBqdiCqdX3hckBOTEFva3KyJINmdGy|IHHzd4V{e2WmIHHzJJN1cW23bHH0bY9vKG:oIHz1Z4ln\XKjc3WgZYN1cX[rdImgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7 M{HsUVE6PTl{MkS1
human HeLaS3 cells MlTrSpVv[3Srb36gZZN{[Xl? M1PCT|IhcA>? M13VPWRqe3CuYXPlcYVvfCCxZjDbN2heTGW6YX3leIhie2:wZTDmdo9uKGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJGhmVGGVMzDj[YxteyCjZoTldkAzKGi{cx?= MYiyN|QxOzB6Mh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Bcl-2 / Bcl-xl / Mcl-1 / Survivin / IAP1 ; 

PubMed: 28696369     


Bcl-xl, Bcl-2, Survivin, Mcl-1, and IAP1 protein levels were determined via Western blot analysis.

Cyclin D1 / Cyclin E / COX-2 / MMP-9 ; 

PubMed: 28696369     


Cyclin D1, Cyclin E, COX-2, and MMP-9 protein levels were determined via Western blot analysis.

Bax / p21; 

PubMed: 28696369     


The protein levels of Bax and p21 were determined via Western blot analysis.

p-JNK / JNK / p-p38 / p38 / p-ERK / ERK ; 

PubMed: 28696369     


The cells were treated with 100 μM of DHAP for the time periods stated; whole-cell extracts were then prepared and analyzed via Western blot analysis for p-p38 (Thr180/Tyr182), p-JNK (Thr183/Tyr185), and p-ERK1/2 (Thr202/Tyr204) by Western blot analysis. To confirm equal protein loading, the immunoblot was stripped and reprobed for JNK, p38, and ERK1/2. The results shown are representative of the three independent experiments

28696369
In vivo Dexamethasone is administered i.m. to pregnant ewes, leads to the following results (1) blood pressure is unchanged; (2) as previously reported in the fetus, sensitivity to endothelin-1 (ET) is increased; (3) acetylcholine-induced relaxation is increased; (4) L-NAME suppressible vasodilatory response to ET is abolished; (5) there is no change in endothelium-independent vasodilatation; and (6) there is no change in eNOS RNA and protein levels, when compared to saline treated controls. [3]

Protocol

Solubility (25°C)

In vitro DMSO 79 mg/mL (201.29 mM)
Ethanol 6 mg/mL (15.28 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+45% PEG 300+H2O
For best results, use promptly after mixing.
13mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 392.46
Formula

C22H29FO5

CAS No. 50-02-2
Storage powder
in solvent
Synonyms N/A

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04091490 Not yet recruiting Drug: Nivolumab Hodgkin Lymphoma State Budgetary Healthcare Institution National Medical Surgical Center N.A. N.I. Pirogov Ministry of Health of Russia January 9 2020 Phase 2
NCT03210545 Not yet recruiting Drug: Dexamethasone Addison Disease Göteborg University September 1 2019 Phase 4
NCT04075721 Not yet recruiting Drug: M3258|Drug: Dexamethasone Multiple Myeloma EMD Serono Research & Development Institute Inc.|Merck KGaA Darmstadt Germany|EMD Serono September 18 2019 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I'm loonkig for Dexamethasone to use in cell culture, would it be better to use the Dexamethasone S1322, the sodiume phosphate S4028 or the acetate S3124?

  • Answer:

    S1322 is the free base form of Dexamethasone, S4028 is the sodium phosphate form and S3124 is the acetate salt. All of them have similar biological activity in tissue culture, however, their suitabilities vary in different solvents. As free base, S1322 has high solubility in DMSO (79mg/ml), poor solubility in water (<1mg/ml), and moderate solubility in ethanol (6mg/ml). As sodium phosphate salt, S4028 has high solubility in water (103mg/ml) but is insoluble in DMSO and ethanol (<1mg/ml). The solubility of S3124 is 87mg/ml in DMSO, <1mg/ml in water, and 20mg/ml in ethanol. Selection can be made based on the customer's experimental setup.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID