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Dexamethasone (MK-125)

Name: Dexamethasone (MK-125)
Brand: Selleck Chemicals
SKU: S1322
Rating: 5 (56 reviews)

Catalog No.S1322 Synonyms: NSC 34521, Hexadecadrol, Prednisolone F

For research use only.

Dexamethasone (MK-125, NSC 34521, Hexadecadrol, Prednisolone F) is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects. Dexamethasone induces autophagy and mitophagy. Dexamethasone is tested in hospitalized patients with COVID-19 and is found to have benefits for critically ill patients.

Dexamethasone (MK-125) Chemical Structure

CAS No. 50-02-2

Selleck's Dexamethasone (MK-125) has been cited by 56 publications

Purity & Quality Control

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Biological Activity

Description

Targets

interleukin receptor ^[1]
(Cell-free assay)

In vitro

Dexamethasone results in decrease in transmonolayer paracellular permeability mainly to sucrose, fluorescein and dextrans of up to 20 KDa in an immortalised rat brain endothelial cell line (GPNT). Dexamethasone results in filamentous actin and the cytoskeleton associated protein cortactin being highly concentrated in the regions of cell-cell contact with few F-actin stress fibres visible within the cytoplasm in cultured rat brain endothelial cells, an observation consistent with a more differentiated barrier phenotype induced by dexamethasone. Dexamethasone treatment has been shown to strongly stimulate the level of the Id-1 protein, which is a serum-inducible helix-loop-helix transcriptional repressor, involved in cell differentiation, and this effect was shown to be associated with reorganisation of ZO-1 to the cell periphery in Con8 mammary epithelial tumor cells. Dexamethasone prevents cytokine-induced enhanced expression of MMP-9 and alterations in the expression of ZO-1 in untreated GPNT monolayers. ^[1] Dexamethasone depletes both basal and TNF-alpha-stimulated GSH levels by down-regulating the gamma-GCS-heavy subunit transcription via a mechanism involving AP-1 (c-Jun) in alveolar epithelial cells. Dexamethasone decreases both basal and stimulated GSH levels (TNF-α-treated) in alveolar epithelial cells (A549), without any change in GSSG. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

human monocytes	MVPGeY5kfGmxbjDhd5NigQ>?	M121fVUh\|ryP	M1fvcFIxKGh?	NX[xRlRESW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCqdX3hckBud26xY4n0[ZMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWFNvaX7keYNm\CCFT2iyJIV5eHKnc4Ppc44h[XRiNTD1UUBi\nSncjCyNEBpenNidYPpcochemGkYnn0JJBwdHmlbH;uZYwh[W62aXLv[JkhUWeJIHL5JHNFWy2SQVfFJIFv[Wy7c3nz	NWjyXZRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OFM6OzVpPkKxPFQ{QTN3PD;hQi=>
rat polymorphonuclear leukocytes	M1;XcWZ2dmO2aX;uJIF{e2G7		MV6yNEBucW6\|	MnzERY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBz[XRicH;sfY1wenCqb371Z4xm[XJibHX1b49kgXSnczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1{fGmvdXzheIVlKG6rdILpZ{BwgGmmZTDwdo9lfWO2aX;uJIFnfGW{IEKwJI1qdnNiYomgS5Jq\XO\|IH3leIhw\CxiSVO1NF0zNjNibl2=	MmXtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3ME[2NlAoRjJ{NUC2OlIxRC:jPh?=
mouse thymocytes	NVrhR\|JyTnWwY4Tpc44h[XO\|YYm=	M2e3UlEh\|ryP	NXuweo8{OjRiaB?=	NUDneVYxUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBud3W\|ZTD0bJlud2O7dHXzJIF{e2W\|c3XkJIF{KGmwdHXycpVkdGWxc3;tZYwhTE6DIH\yZYdu\W62YYTpc44h[XRiMTD1UUBi\nSncjCyOEBpenNiYomgZYdiem:\|ZTDn[Ywh\WynY4Tyc5Bpd3Knc3nz	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd7NEe0PEc,OjR5OUS3OFg9N2F-
T-cell	M1PITGZ2dmO2aX;uJIF{e2G7			NILlbJBKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6\|dDDUJINmdGxicILvcIln\XKjdHnvckB2e2mwZzDjc45k[W6jdnHsbY4hSSC\|dHnteYxifGWmIH3pZ4Uhe3CuZXXuJINmdGy\|LDDJR\|UxKD1iMD6wNFUh\|ryPLh?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTJ{OUe2O{c,OTF{Mkm3Olc9N2F-
PBMC	NEL1RlFHfW6ldHnvckBie3OjeR?=			NHfv[GRKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6\|dDDUUmYu[WyyaHGgdJJw\HWldHnvckBnem:vIHj1cYFvKHCncnnwbIVz[WxiYnzvc4QhdW:wb371Z4xm[XJiY3XscJMhe3SrbYXsZZRm\CCkeTDMVHMtKEmFNUCgQUAxNjBzIN88UU4>	NYfLVYZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGyNlk4PjdpPkGxNlI6PzZ5PD;hQi=>
PBMC	NYn1TmJzTnWwY4Tpc44h[XO\|YYm=			NH[1RYFKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6\|dDDndo94fGhib3[gUHBUKHO2aX31cIF1\WRiaIXtZY4heGW{aYDo[ZJidCCkbH;v[EBud26xboXjcIVieiClZXzsd{whUUN3MDC9JFAvODRizszNMi=>	NHfmcoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUKyPVc3Pyd-MUGyNlk4Pjd:L3G+
RAW264.7	NYCyfWM4SW62aXnu[oxidW2jdH;yfUBie3OjeR?=		Mn7tNlQhcHK\|	NGKzeJhCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKEySUz3zeIlufWyjdHXkJI1wfXOnIGLBW\|I3PC55IHPlcIx{KGG\|c3Xzd4VlKGG\|IHXm[oVkfCCxbjDUUmZidHCqYTDmc5Ju[XSrb36gdJJmfHKnYYTl[EAyKGi{IHLl[o9z\SCOUGOgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEK0JIhzeyxiSVO1NEA:KDBwNzFOwG0v	Mo\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7MEi5PFQoRjFzOUC4PVg1RC:jPh?=
N9	M4fJeGZ2dmO2aX;uJIF{e2G7			NVfYWpNwUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KFSQRj3hcJBp[SC{ZXzlZZNmKGmwIF65JINmdGy\|IHnuJJRp\SC\|dYDldo5ifGGwdDygTWM2OCB;IECuNFc1KM7:TT6=	M1TX[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN\|k2Lz5zMkO2NVM6PTxxYU6=
RAW 264.7	MXPGeY5kfGmxbjDhd5NigQ>?			NWfCZ45wUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KFSQRj3hcJBp[SC{ZXzlZZNmKGmwIGLBW{AzPjRwNzDj[YxteyCrbjD0bIUhe3WyZYLuZZRidnRuIFnDOVAhRSByLkSyJO69VS5?	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjN4MUO5OUc,OTJ\|NkGzPVU9N2F-
A549	MWDGeY5kfGmxbjDhd5NigQ>?	MVmxNEB2VQ>?		MWfBcpRi\2:waYP0JIFkfGm4aYT5JIZweiCJbIXjc4NwenSrY3;p[EBz\WOncITvdkBqdiCPTWTWJJRz[W6\|YXP0bZZifGmxbjDhd5NigSCrbjDoeY1idiCDNUS5JIx2dmdiZYDpeIhmdGmjbDDj[YxteyCjdDCxNEB2VSxiSVO1NEA:KDFyIN88UU4>	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl7OUm4PUc,OTV7OUm5PFk9N2F-
A549	Mn;xSpVv[3Srb36gZZN{[Xl?			M4XhWWFkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJGE2PDliY3XscJMh[XO\|ZYPz[YQh[XNiZX\m[YN1KG:wIFfSSUBxem:vb4TldkBz\XOyb37z[UBjgSCudXPp[oVz[XOnIILldI9zfGW{IHHzd4F6NCCHQ{WwJF0hOC5yMEGg{txONg>?	MmDtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4NEOwOlAoRjF4NkSzNFYxRC:jPh?=
A549	NX22bmRwTnWwY4Tpc44h[XO\|YYm=			MWHB[49vcXO2IHHjeIl3cXS7IHH0JGdTKGG\|c3Xzd4VlKGG\|IF3NWHYudWWmaXH0[YQhfHKjboPhZ5RqfmG2aX;uJI9nKHKnbnnscIEhdHWlaX\ldoF{\SCpZX7lJIlvKGi3bXHuJGE2PDliY3XscJMhemWuYYTpeoUhfG9iRHX4ZY1mfGijc3;u[UwhTUN3MDC9JFAvODB3MEGyJO69VS5?	NHj4coc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkiyNVc5OSd-MU[4NlE4QDF:L3G+
A549	NXq2Uo5CTnWwY4Tpc44h[XO\|YYm=			MVzB[49vcXO2IHHjeIl3cXS7IHH0JGdTKGG\|c3Xzd4VlKGG\|IF7GMYtieHCjQj3t[YRq[XSnZDD0doFve3KncILld5Nqd25ib3[gd4VkemW2ZXSgdIxi[2WwdHHsJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGenbnWgbY4hcHWvYX6gRVU1QSClZXzsd{whUUN3MDC9JFAvODBzMUe1JO69VS5?	MlLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ6MkG3PFEoRjF4OEKxO\|gyRC:jPh?=
HFF	M{\veWZ2dmO2aX;uJIF{e2G7			MYHJcoR2[3Srb36gc4Yh[XKxbXH0ZZNmKGGldHn2bZR6KGmwIFjGSkBk\WyuczDhd5Nme3OnZDDhd{Bxem:mdXP0bY9vKG:oIHLleIEh\XO2cnHkbY9tKGK7IHHyc41ifGG\|ZTDhd5NigSxiRVO1NEA:KDBwMECxPUDPxE1w	MlvKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzOEGxO\|IoRjF5MUixNVczRC:jPh?=
HeLa	MlrvSpVv[3Srb36gZZN{[Xl?			MkDyRYN1cX[jdHnvckBw\iCPTWTWJIlvKEinTHGgZ4VtdHNibXXhd5Vz\WRiYomgcJVkcW[ncnHz[UBi[3Srdnn0fUwhTUN3MDC9JFAvODF5IN88UU4>	M2TmfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUixNVczLz5zN{G4NVE4OjxxYU6=
HFF	MlLLSpVv[3Srb36gZZN{[Xl?			M4jKeWlvcGmkaYTpc44hd2ZiSVyxMZN1cW23bHH0[YQhUUx4IIDyc4R2[3Srb36gbY4hUE[IIHPlcIx{NCCLQ{WwJF0hOC5yMEC1NUDPxE1w	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF6MUG3Nkc,OTdzOEGxO\|I9N2F-
RAW 264.7	M4nCUmZ2dmO2aX;uJIF{e2G7		NWrU[mtFOjRiaILz	NV3EXm5GUW6qaXLpeIlwdiCxZjDpcpRmem[ncn;uJIdidW2jLYP0bY12dGG2ZXSgUm8heHKxZIXjeIlwdiCrbjDSRXchOjZ2LkegZ4VtdHNiYX\0[ZIhOjRiaILzMEBKSzVyIE2gNE4xOiEQvF2u	M4fJWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{[3NVI1Lz5zN{O2O\|EzPDxxYU6=
RAW264.7	MVLGeY5kfGmxbjDhd5NigQ>?		NViydG9TOiCqcoO=	NHLvTGJKdmirYnn0bY9vKG:oIFPPXFIh\XiycnXzd4lwdiCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDh[pRmeiB{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRyN{K3O{c,OTd2MEeyO\|c9N2F-
RAW264.7	NIXqSHVHfW6ldHnvckBie3OjeR?=		MUCyJIhzew>?	NVT6V2FjUW6qaXLpeIlwdiCxZjDtVGdGWzFiZYjwdoV{e2mxbjDpckBNWFNvc4TpcZVt[XSnZDDtc5V{\SCUQWeyOlQvPyClZXzsd{Bi\nSncjCyJIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM>	NFfTVWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SwO\|I4Pyd-MUe0NFczPzd:L3G+
A549	NEXQR3hHfW6ldHnvckBie3OjeR?=			MVfB[49vcXO2IHHjeIl3cXS7IHH0JIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHnuJIh2dWGwIFG1OFkh[2WubIOgZpkhVU2WVjD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjByNUO3JO69VS5?	M1z2UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkG2N\|k2Lz5zN{[xOlM6PTxxYU6=
A549	M{HxdGZ2dmO2aX;uJIF{e2G7			NWHO[3ZOSWexbnnzeEBi[3Srdnn0fUBifCCpbIXjc4NwenSrY3;p[EBz\WOncITvdkBqdiCqdX3hckBCPTR7IHPlcIx{KGK7IF7GMYtieHCjQjD0doFve3KncILld5Nqd25iYYPzZZktKEmFNUCgQUAxNjByMTFOwG0v	NVHFeJVMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NVY{QTVpPkG3OlE3Ozl3PD;hQi=>
A549	M1;WVGZ2dmO2aX;uJIF{e2G7			NYHkS\|VpSW62YXfvcol{fCCjY4Tpeol1gSCjdDDncJVkd2OxcoTpZ49q\CC{ZXPldJRweiCrbjDoeY1idiCDNUS5JINmdGy\|IITyZY5{\mWldHXkJJdqfGhiTV3UWkBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDk[ZhidWW2aHHzc45mNWmwZIXj[YQh[WO2aY\heIlwdixiSVO1NEA:KDFizszNMi=>	NX3zPWVWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NVY{QTVpPkG3OlE3Ozl3PD;hQi=>
HFF	MX;BcpRqcW6obHHtcYF1d3K7IHHzd4F6			MX3BcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiSVyxJJN1cW23bHH0[YQhUUx4IIDyc4R2[3Srb36gbY4hUE[IIHPlcIx{KGK7IITyZY5{emWycnXzd4lwdiCjc4PhfUwhTUN3MDC9JFAvODByNTFOwG0v	NHLycpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[5NlUyQSd-MUe2PVI2OTl:L3G+
HFF	MX\BcpRqcW6obHHtcYF1d3K7IHHzd4F6			NYPXPIZUSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBie3Onc4Pl[EBieyC3cILl[5Vt[XSrb36gc4Yh[XKxbXH0ZZNmKGmwIFjGSkBk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCwNkDPxE1w	MoTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4OUK1NVkoRjF5NkmyOVE6RC:jPh?=
CV1	Mn3zSpVv[3Srb36gZZN{[Xl?			NWjYTmNsSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBIWiCneIDy[ZN{\WRiaX6gR3YyKGOnbHzzJIJ6KEeURTDhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wNFAzKM7:TT6=	M4\QWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{C1N\|YzLz5zN{ewOVM3OjxxYU6=
NHDFneo	MWLGeY5kfGmxbjDhd5NigQ>?			NVXHblBbSWO2aY\peJkh[XRiaIXtZY4hT1JiZYjwdoV{e2WmIHnuJG5JTE[wZX:gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCLTD22JJBzd2S3Y4Tpc44tKEmFNUCgQUAxNjByMUSg{txONg>?	NVXoTppQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NFU{PjJpPkG3O\|A2OzZ{PD;hQi=>
RPMI8226	MlTvRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M2\YPVQh\GG7cx?=	MmnCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCUUF3JPFIzPiClZXzsd{Bi\nSncjC0JIRigXNuIFnDOVAhRSByLkCwOlUh\|ryPLh?=	NYjRTms6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NFU{PjJpPkG3O\|A2OzZ{PD;hQi=>
J774A.1	MY\GeY5kfGmxbjDhd5NigQ>?			MlnYTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmZidHCqYTDwdo9lfWO2aX;uJIlvKG2xdYPlJGo4PzSDLkGgZ4VtdHNuIFnDOVAhRSByLkC0PEDPxE1w	NVv6SIh7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PVI6PDFpPkG3PFkzQTRzPD;hQi=>
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Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot

Bcl-2 / Bcl-xl / Mcl-1 / Survivin / IAP1 ; Cyclin D1 / Cyclin E / COX-2 / MMP-9 ; Bax / p21 ; p-JNK / JNK / p-p38 / p38 / p-ERK / ERK

28696369

In vivo

Dexamethasone is administered i.m. to pregnant ewes, leads to the following results (1) blood pressure is unchanged; (2) as previously reported in the fetus, sensitivity to endothelin-1 (ET) is increased; (3) acetylcholine-induced relaxation is increased; (4) L-NAME suppressible vasodilatory response to ET is abolished; (5) there is no change in endothelium-independent vasodilatation; and (6) there is no change in eNOS RNA and protein levels, when compared to saline treated controls. ^[3]

Protocol (from reference)

References

+ Expand

Solubility (25°C)

In vitro	DMSO	79 mg/mL (201.29 mM)
	Water	Insoluble
	Ethanol	'''6 mg/mL
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 5% DMSO+45% PEG 300+H2O For best results, use promptly after mixing. Click to purchase: PEG300	13mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	392.46
Formula	C₂₂H₂₉FO₅
CAS No.	50-02-2
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC1CC2C3CCC4=CC(=O)C=CC4(C3(C(CC2(C1(C(=O)CO)O)C)O)F)C

Download Dexamethasone (MK-125) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05142098	Not yet recruiting	Drug: Dexamethasone\|Drug: Etoricoxib 60 mg	Effect of Drug	Dow University of Health Sciences	January 1 2022	Early Phase 1
NCT05170789	Not yet recruiting	Drug: Elotuzumab Selinexor and Dexamethasone (ESd)	Relapsed Multiple Myeloma\|Refractory Multiple Myeloma	Tulane University School of Medicine\|Karyopharm Therapeutics Inc\|Tulane University	January 7 2022	Phase 2
NCT05136781	Recruiting	--	Thoracic Surgery\|Anesthesiology\|Corticosteroids\|Respiratory Complication	Centre Hospitalier Universitaire Amiens	November 16 2021	--

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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Frequently Asked Questions

Question 1:
I'm loonkig for Dexamethasone to use in cell culture, would it be better to use the Dexamethasone S1322, the sodiume phosphate S4028 or the acetate S3124?

Answer:
S1322 is the free base form of Dexamethasone, S4028 is the sodium phosphate form and S3124 is the acetate salt. All of them have similar biological activity in tissue culture, however, their suitabilities vary in different solvents. As free base, S1322 has high solubility in DMSO (79mg/ml), poor solubility in water (<1mg/ml), and moderate solubility in ethanol (6mg/ml). As sodium phosphate salt, S4028 has high solubility in water (103mg/ml) but is insoluble in DMSO and ethanol (<1mg/ml). The solubility of S3124 is 87mg/ml in DMSO, <1mg/ml in water, and 20mg/ml in ethanol. Selection can be made based on the customer's experimental setup.

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