Calcitriol

Catalog No.S1466 Synonyms: RO215535, Topitriol

Calcitriol Chemical Structure

Molecular Weight(MW): 416.64

Calcitriol is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol.

Size Price Stock Quantity  
In DMSO USD 500 In stock
USD 120 In stock
USD 270 In stock
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Cited by 9 Publications

3 Customer Reviews

  • To chemically induce klotho expression, THP-1 cells were treated with Calcitriol. The effects were evaluated on the gene level with real time PCR from 5 to 480 min after treatment and on the protein level with western blot after 48 h and 72 h.

    Mol Cell Endocrinol, 2018, 470:1-13. Calcitriol purchased from Selleck.

    Calcitriol alleviates inflammation in vivo accompanied by upregulated A20. c HE staining, CD3+ lymphocyte and F4/80+ macrophage staining. Values are shown as mean ± SD, n = 5 per group. **P < 0.01 among groups. ΔΔP < 0.01 versus control.

    Inflammation, 2017, 40(6):1884-1893. Calcitriol purchased from Selleck.

  • Mireia Vila Gasull University of Porto. 2011;Mireia Vila Gasull . Calcitriol purchased from Selleck.

Purity & Quality Control

Choose Selective Vitamin Inhibitors

Biological Activity

Description Calcitriol is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol.
Targets
vitamin D receptor [1]
(Cell-free assay)
In vitro

Calcitriol is a potent inhibitor of PHA-induced lymphocyte proliferation, achieving 70% inhibition of tritiated thymidine incorporation after 72 h in culture. Calcitriol suppresses interleukin-2 (IL-2) production by PHA-stimulated peripheral blood mononuclear cells in a concentration-dependent fashion. [1] Calcitriol increases the concentration of intracellular calcium ([Ca2+]i) within 5 s by mobilizing calcium from the endoplasmic reticulum and the formation of inositol 1,4, 5-trisphosphate and diacylglycerol. [2] Calcitriol can inhibit the proliferation and promote the differentiation of human prostate adenocarcinoma cells. Calcitriol causes a selective decrease in the secreted levels of type IV collagenases (MMP-2 and MMP-9). [3] Calcitriol has antiproliferative activity in squamous cell carcinoma and prostatic adenocarcinoma and enhances the antitumor activity of platinum-based agents. Calcitriol prior to paclitaxel significantly reduces clonogenic survival compared with either agent alone in the murine squamous cell carcinoma and PC-3 cells. [4] Calcitriol is a potent anti-proliferative agent in a wide variety of malignant cell types. Calcitriol effects are associated with an increase in G0/G1 arrest, induction of apoptosis and differentiation, modulation of expression of growth factor receptors. Calcitriol potentiates the antitumor effects of many cytotoxic agents and inhibits motility and invasiveness of tumor cells and formation of new blood vessels. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HCT116 M3LKWmN6fG:2b4jpZ:Kh[XO|YYm= M1v2d|EzOCCq NXLnPGNzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwh[2:3boSgZYZ1\XJiMUKwJIhzeyxiSVO1NF0y\S1yNTFOwG0> NXn0bo5qOjJ7Mk[wOlg>
human HOS cells NF;IfYxHfW6ldHnvckBie3OjeR?= MlHtNlQhcA>? M2LR[WFkfGm4aYT5JIF1KGi3bXHuJHZFWiCneIDy[ZN{\WRiaX6gbJVu[W5iSF;TJINmdGy|IITyZY5{\mWldHXkJJdqfGhicFfMN{1pV2NuIIDDSG5CNWiYRGKgZY5lKHCqUlytWGsh[XO|ZYPz[YQh[XNiYYPz[ZN{\WRiYYOgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gcYVie3W{ZXSgNlQhcHK|IIDvd5QhcW6oZXP0bY9vKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2x[U0xPSEQvF2= M3rxUVIyQDh7M{O0
human HOS/SF cells MYnGeY5kfGmxbjDhd5NigQ>? NGXyTZYzPCCq NHTXVVlVemGwc3HjeIl3[XSrb36gc4YhXkSULX3l[IlifGWmIH;zeIVw[2GuY3nuJJBzd22xdHXyJIlvKGi3bXHuJGhQWy:VRjDj[YxteyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTJwNnWtNFUh|ryP M1GxN|I1QTByN{K4
human PBMC MVPGeY5kfGmxbjDhd5NigQ>? NEfhdGszPCCq Mmr4TY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmZidHCqYTDwdo9lfWO2aX;uJIlvKGi3bXHuJHBDVUNiYX\0[ZIhOjRiaILzJIJ6KEWOSWPBMEBKSzV;Mj65[U0xPSEQvF2= NWC4O2ZROTl|MEmxOVU>
mouse LM05e cells NIq3UoxRem:uaX\ldoF1cW:wIHHzd4F6 MWK3NkBp NHHJdnpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFzNNFVmKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhdnWvYnXyJIFnfGW{IEeyJIhzeyCkeTDXV3Qh[XO|YYmsJGlEPTB;M3WtNFUh|ryP NWnKeXVPOjR7MECzN|k>
rat osteosarcoma cells M{PwVmZ2dmO2aX;uJIF{e2G7 MkjITY5lfWO2aX;uJI9nKF[GUj3t[YRq[XSnZDCyOE1pgWS{b4j5cIF{\SC2cnHud4NzcXC2aX;uJIlvKHKjdDDvd5Rmd3OjcnPvcYEh[2WubIOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFSFUxRTBwMjDuUS=> NU[0U2F[OjF7MEKyN|U>
rat ROS 17/2.8 cells MonRSpVv[3Srb36gZZN{[Xl? NVnlblZyOTZiaB?= MVPBZ5Rqfmm2eTDheEBXTFJiaX6gdoF1KFKRUzCxO{8zNjhiY3XscJMh[XO|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25ib3[gNlQucHmmcn;4fYxie2ViZ3Xu[UBxem:vb4TldkBi\nSncjCxOkBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvOiCwTR?= NHzHS|UyQTF4N{i5Ny=>
COS7 cells MluxSpVv[3Srb36gZZN{[Xl? NFy3N2wzPCCq MnnhRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDWSHIhcW5iQ1;TO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZidILhcpNkemmydHnvcoFtKHS{YX7zZYN1cX[jdHnvckBi\nSncjCyOEBpenNiYomgcY92e2Vib4P0[Y9xd262aX6gcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvPSCwTR?= NVrTPHpHOTd7MESzO|A>
mouse SVEC cells M1;Rd3Bzd2yrZnXyZZRqd25iYYPzZZk> MYm3NkBp MlOwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCVVlXDJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygcpVu[mW{IHHmeIVzKDd{IHjyd{BjgSCZU2SgZZN{[XluIFnDOVA:OC53MjDuUS=> NH\FUZAzPDlyMEOzPS=>
CV1 cells NVf5dpFtTnWwY4Tpc44h[XO|YYm= M3XxXWFkfGm4aYT5JI9vKGi3bXHuJHZFWi2vZXTpZZRm\CC2cnHud4NzcXC2aX;uJI9nKG:|dHXvZ4Ft[2mwIG\EVmUh\nW|ZXSgeI8hfGi7bXnkbY5mKGurbnHz[UBxem:vb4Tldk9oem:5dHigbI9zdW:wZTDy[ZBwenSncjDn[Y5mKGmwIFPWNUBk\WyuczygSWQ2OD1yLkegcm0> M4jJZ|E4OTR7OEiw
COS1 cells M1P4Z2Z2dmO2aX;uJIF{e2G7 NEjWVYpFcXOybHHj[Y1mdnRib3[gNU1idHCqYTygNlUuME:KKWuzTH1FOyCocn;tJIh2dWGwIG\EVkBmgHC{ZYPz[YQhcW5iQ1;TNUBk\WyuczygTWM2OD1yLkigcm0> NHLaUZcyPzl{NE[xOi=>
human PBMC MnXCSpVv[3Srb36gZZN{[Xl? NUTKXG4zOjRiaB?= MWjJcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIFnMNVIwOjOyNECgdJJw\HWldHnvckBqdiCqdX3hckBRSk2FIHHmeIVzKDJ2IHjyd{BjgSCHTFnTRUwhUUN3ME2xJI5O MVuxPVMxQTF3NR?=
HEK293 cells M37jRWZ2dmO2aX;uJIF{e2G7 MWiyOEBp MXHB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIG\EVkBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIITyZY5{[3KrcITpc45idCC2cnHud4FkfGm4YYTpc44h[W[2ZYKgNlQhcHK|IHL5JI1wfXOnIH;zeIVweG:wdHnuJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0yKG6P NXXEWW5QOTd7MESzO|A>
human HL60 cells MYfGeY5kfGmxbjDhd5NigQ>? MWnJcoR2[3Srb36gc4YhcHWvYX6gTGw3OCCmaX\m[ZJmdnSrYYTpc44h[XO|ZYPz[YQh[XNiY3XscJMhemWmdXPpcochdmm2cn:gZox2\SC2ZYTyZZpwdGm3bTygSWQ2OD1zIH7N MlXWNlE6ODJ{M{W=
human HeLa cells NFHuU4lHfW6ldHnvckBie3OjeR?= NXH4b5B7OTZiaB?= MX3UdoFve2GldHn2ZZRqd25ib3[gS2FNPC2odYPl[EBXTFJibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGGodHXyJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OSCwTR?= NH3jcYczOjl3N{izOC=>
human T47D cells MXHQdo9tcW[ncnH0bY9vKGG|c3H5 NFP4e444OiCq NXLMdJZESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhdnWvYnXyJIFnfGW{IEeyJIhzeyCkeTDXV3Qh[XO|YYmsJGlEPTB;MT6zJI5O NWrvNXl4OjR7MECzN|k>
human peripheral blood mononuclear cells MX7Qdo9tcW[ncnH0bY9vKGG|c3H5 NW\wTVNOUW5idnn0do8hcW6qaXLpeIlwdiCxZjDwbJl1d2inbXHn[4x2fGmwaX7pck1qdmS3Y3XkJJN1cW23bHH0bY9vKG:oIHj1cYFvKHCncnnwbIVz[WxiYnzvc4QhdW:wb371Z4xm[XJiY3XscJMheHKxbHnm[ZJifGmxbjygSWQ2OD1{Lkegcm0> MmWzNVExODByMUS=
human Caco-2 cells MkLOSpVv[3Srb36gZZN{[Xl? MoLJSYZn\WO2IH;uJHZFWiC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkhcW5iaIXtZY4hS2Glbz2yJINmdGy|IHnuJJBz\XOnbnPlJI9nKDVnIH\leIFtKGOjbH[gd4VzfW1uIFXDOVA:Oy53MjDuUS=> MnO0NVgxPTR{M{C=
human MCF7 cells NIDBcItHfW6ldHnvckBie3OjeR?= MljaRYdwdmm|dDDhZ5Rqfmm2eTDheEB7\WK{YX\pd4gh\2GuND3WSHIhVEKGIHX4dJJme3OnZDDpckBpfW2jbjDNR2Y4KGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBj[XOnZDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUC9OU42KG6P NHnGbW4zOjF6MEizOy=>
human THP1 cells MUfGeY5kfGmxbjDhd5NigQ>? M2DZNmFkfGm4YYTpc44hd2ZiVlTSJIlvKGi3bXHuJHRJWDFiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZ4F1cGWuaXPp[IlvKGGwdHntbYNzd2KrYXygdIVxfGmmZTDtVm5CKGW6cILld5Nqd25iYomgVnQuWEOULDDFR|UxRTFyIH7N Mk[xNVk{ODlzNUW=
human HaCaT cells Mkm2SpVv[3Srb36gZZN{[Xl? MWCyOEBp M{juPHRz[W6|YXP0bZZifGmxbjDv[kBXTFJiaX6gbJVu[W5iSHHDZXQh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlMzOTVizszN NI\2cVQzPjN4N{CxPS=>
human HN12 cells NGjDO4VRem:uaX\ldoF1cW:wIHHzd4F6 MVW3NkBp NIDkTItCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjONVIh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEBvfW2kZYKgZYZ1\XJiN{KgbJJ{KGK7IGfTWEBie3OjeTygTWM2OD1yLkW1N{DPxE1? M2e4OlI1QTByM{O5
human HaCaT cells MmfpSpVv[3Srb36gZZN{[Xl? NYPzeHd[UW6qaXLpeIlwdiCxZjDjc4xwdnliZn;ycYF1cW:wIH;mJIh2dWGwIFjhR4FVKGOnbHzz NXLtdlM1OjJ2MESzNlY>
human HCT116 cells M{nM[WZ2dmO2aX;uJIF{e2G7 NXXicYNyOjBvNECg{txO M1rP[VI1KGh? MknvRYdwdmm|dDDhZ5Rqfmm2eTDheEBTSVJiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJINwNWW6cILld5NqdmdiUlHSSUBifCB{MDD0c{A1OCC3TTDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5 NHHpNG4zOzJ5NkS0PS=>
mouse C3H10T1/2 cells NETUeXZHfW6ldHnvckBie3OjeR?= NUjBXXJqOC53LUWg{txO MYmyOEBp NHL3SI1KdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIFOzTFExXDFxMjDj[YxteyCjc4Pld5Nm\CCjczDkc5dvKHKnZ4XsZZRqd25ib3[gS4xqOSCjdDCwMlUhfG9iNTD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOUIHHuZYx6e2m| NWnmSJM6OjJ{Mk[2OVc>
human A498 cells MWnGeY5kfGmxbjDhd5NigQ>? MYOwMlAyKM7:TR?= MkTGOEBp MXXJcoR2[3Srb36gc4YhS1mSMkSgcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIFG0PVgh[2WubIOgZZQhOC5yMTFOwG0h[W[2ZYKgOEBpenNiYomgVnQuWEOUIHHuZYx6e2m| M1G1eFIyOzl{OUiz

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 83 mg/mL (199.21 mM)
Ethanol 83 mg/mL (199.21 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 416.64
Formula

C27H44O3

CAS No. 32222-06-3
Storage powder
in solvent
Synonyms RO215535, Topitriol

Bio Calculators

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03563235 Not yet recruiting Postmenopausal Osteoporoses Fujian Academy of Traditional Chinese Medicine September 1 2019 Early Phase 1
NCT03563235 Not yet recruiting Postmenopausal Osteoporoses Fujian Academy of Traditional Chinese Medicine September 1 2019 Early Phase 1
NCT03748966 Recruiting X-linked Hypophosphatemia|Hypophosphatemic Rickets|Hypophosphatemic Rickets X-Linked Dominant Massachusetts General Hospital|National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) December 1 2018 Early Phase 1
NCT03748966 Recruiting X-linked Hypophosphatemia|Hypophosphatemic Rickets|Hypophosphatemic Rickets X-Linked Dominant Massachusetts General Hospital|National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) December 1 2018 Early Phase 1
NCT03249012 Recruiting Hypoparathyroidism Postprocedural|Quality of Life CHU de Quebec-Universite Laval September 1 2017 Not Applicable
NCT03249012 Recruiting Hypoparathyroidism Postprocedural|Quality of Life CHU de Quebec-Universite Laval September 1 2017 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID