Calcitriol (RO215535)

Catalog No.S1466 Synonyms: Topitriol, 1,25-Dihydroxyvitamin D3

For research use only.

Calcitriol (RO215535, Topitriol, 1,25-Dihydroxyvitamin D3) is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol. Solutions are best fresh-prepared.

Calcitriol (RO215535) Chemical Structure

CAS No. 32222-06-3

Selleck's Calcitriol (RO215535) has been cited by 42 publications

Purity & Quality Control

Choose Selective Vitamin Inhibitors

Biological Activity

Description Calcitriol (RO215535, Topitriol, 1,25-Dihydroxyvitamin D3) is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol. Solutions are best fresh-prepared.
Targets
vitamin D receptor [1]
(Cell-free assay)
In vitro

Calcitriol is a potent inhibitor of PHA-induced lymphocyte proliferation, achieving 70% inhibition of tritiated thymidine incorporation after 72 h in culture. Calcitriol suppresses interleukin-2 (IL-2) production by PHA-stimulated peripheral blood mononuclear cells in a concentration-dependent fashion. [1] Calcitriol increases the concentration of intracellular calcium ([Ca2+]i) within 5 s by mobilizing calcium from the endoplasmic reticulum and the formation of inositol 1,4, 5-trisphosphate and diacylglycerol. [2] Calcitriol can inhibit the proliferation and promote the differentiation of human prostate adenocarcinoma cells. Calcitriol causes a selective decrease in the secreted levels of type IV collagenases (MMP-2 and MMP-9). [3] Calcitriol has antiproliferative activity in squamous cell carcinoma and prostatic adenocarcinoma and enhances the antitumor activity of platinum-based agents. Calcitriol prior to paclitaxel significantly reduces clonogenic survival compared with either agent alone in the murine squamous cell carcinoma and PC-3 cells. [4] Calcitriol is a potent anti-proliferative agent in a wide variety of malignant cell types. Calcitriol effects are associated with an increase in G0/G1 arrest, induction of apoptosis and differentiation, modulation of expression of growth factor receptors. Calcitriol potentiates the antitumor effects of many cytotoxic agents and inhibits motility and invasiveness of tumor cells and formation of new blood vessels. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HCT116 NYrhSY9VS3m2b4TvfIlkyqCjc4PhfS=> NXXwTmlGOTJyIHi= NIf1e3FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEBkd3WwdDDh[pRmeiBzMkCgbJJ{NCCLQ{WwQVFmNTB3IN88US=> M2PWWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUK2NFY5Lz5{MkmyOlA3QDxxYU6=
HOS NWD6Vo11TnWwY4Tpc44h[XO|YYm= M4C3UGFkfGm4aYT5JIF1KGi3bXHuJHZFWiCneIDy[ZN{\WRiaX6gbJVu[W5iSF;TJINmdGy|IITyZY5{\mWldHXkJJdqfGhicFfMN{1pV2NuIIDDSG5CNWiYRGKgZY5lKHCqUlytWGsh[XO|ZYPz[YQh[XNiYYPz[ZN{\WRiYYOgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gcYVie3W{ZXSgNlQhcHK|IIDvd5QhcW6oZXP0bY9vKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODByMEGg{txONg>? NVPON5F2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4PFk{OzRpPkKxPFg6OzN2PD;hQi=>
SK-MEL-188 NEX4UnJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2LncVQ5KGi{cx?= M3ftWWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHNMNU2HTD2xPFgh[2WubIOgcYVie3W{ZXSgZYZ1\XJiNEigbJJ{KGK7IF3UV{9RVVNiYYPzZZktKEmFNUCgQUAxNjByMECxJO69VS5? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB5MEe3PUc,OjdyN{C3O|k9N2F-
osteosarcoma cells NUewdGt{TnWwY4Tpc44h[XO|YYm= NUGwOVVLTW[oZXP0JI9vKF[GUjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliaX6gbJVu[W5ib4P0[Y9{[XKlb33hJINmdGy|LDDFR|UxKD1iMD6wNFAxOTB4IN88UU4> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB3NEKzNEc,OThyNUSyN|A9N2F-
HOS/SF NHfNW3lHfW6ldHnvckBie3OjeR?= NUnrO|VZOjRiaILz MX\UdoFve2GldHn2ZZRqd25ib3[gWmRTNW2nZHnheIVlKG:|dHXvZ4Ft[2mwIIDyc41wfGW{IHnuJIh2dWGwIFjPV{9UTiClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMECwNlYh|ryPLh?= M2nPUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwO|I5Lz5{NEmwNFczQDxxYU6=
LM05e NF\qeo1CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M1zTOFczKGi{cx?= NIW3fWhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFzNNFVmKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhdnWvYnXyJIFnfGW{IEeyJIhzeyCkeTDXV3Qh[XO|YYmsJGlEPTBiPTCwMlAxODB|IN88UU4> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEOzPUc,OjR7MECzN|k9N2F-
HEK293 M4rlemZ2dmO2aX;uJIF{e2G7 NEXNXZlVemGwc4LldJJme3Orb36gc4YhXlBzNj30ZYdo\WRiVlTSJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDoZZJjd3KrbnegdGNOYC2JQVy0MW5Ed1JiYX7kJG1JOTByKGXBV{khYCB2dHutUHVEKHKncH;yeIVzKHCuYYPtbYQh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gUmNwWiC{ZXPyeYl1dWWwdDDifUBj\XSjLXfhcIFkfG:|aXThd4UhemWyb4L0[ZIh\2WwZTDiZZNm\CCvYX3tZYxq[W5idIfvMYh6[nKrZDDhd5NigSxiSVO1NEA:KDBwMECwNFUh|ryPLh?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTl6OUixO{c,Ojl7OEm4NVc9N2F-
HEK293 NHO2RZJHfW6ldHnvckBie3OjeR?= M{PLXXRz[W6|YXP0bZZifGmxbjDv[kBXWDF4LYTh[4dm\CCYRGKgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHjhdoJwemmwZzDwR21ZNUeDTEStVnhT[WyyaHGgZY5lKE2KMUCwLHVCWyliWDC0eIsuVFWFIILldI9zfGW{IIDsZZNucWRiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcW62ZYLhZ5Rqd25id3n0bEBTYFKjbIDoZUBjgSCkZYThMYdidGGldH;zbYRie2VicnXwc5J1\XJiZ3Xu[UBj[XOnZDDtZY1u[WyrYX6geJdwNSxiRVO1NEA:KDBwMECwNFch|ryPLh?= NG\obXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUm4PVgyPyd-Mkm5PFk5OTd:L3G+
HEK293T NIGyNpNHfW6ldHnvckBie3OjeR?= NXvjPHFbSWexbnnzeEBi[3Srdnn0fUBifCCJQVy0JGRPSS2kaX7kbY5oKGSxbXHpckBnfXOnZDDWSHIhMHWwa37ve44hd3KrZ3nuLUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJHVCWy2kbHGgTGVMKDJ7M2SgZ4VtdHNiYYPz[ZN{\WRiYYOgZoV1[S2uYXP0ZY1ie2VidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYomgSnJGXC2kYYPl[EBI\W6nQlzBfoVzKGG|c3H5MEBKSzVyIE2gNE4xODByOUWzJO69VS5? M4HSd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUS0Olk4Lz5|MEG0OFY6PzxxYU6=
HEK293 NGXufYJHfW6ldHnvckBie3OjeR?= M3TZbWFod26rc4SgZYN1cX[rdImgZZQhXkSUIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyClb4TyZY5{\mWldHXkJJdqfGhiTlPvVkBie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDOR49TKHKnY4L1bZRu\W62IHL5JJR4dy2qeXLybYQh[XO|YYmsJGlEPTBiPTCwMlAxODFizszNMi=> NVjrfXd2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2NVM1OjBpPkK2OlE{PDJyPD;hQi=>
HEK293 NIDOPWRHfW6ldHnvckBie3OjeR?= MlHONVYhfG9iMkSgbJJ{ NHXBVFVC\2:waYP0JIFkfGm4aYT5JIF1KF[GUjDpckBpfW2jbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGGodHXyJFE3KHSxIEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjByMEGyJO69VS5? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZzM{SyNEc,OjZ4MUO0NlA9N2F-
ROS 17/2.8 MYTGeY5kfGmxbjDhd5NigQ>? NFvlO5YyPiCqcoO= NXu0NlhwSWO2aY\peJkh[XRiVlTSJIlvKHKjdDDSU3MhOTdxMj64JINmdGy|IHHzd4V{e2WmIHHzJJRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJI9nKDJ2LXj5[JJwgHmuYYPlJIdmdmVicILvcY91\XJiYX\0[ZIhOTZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFAxOiEQvF2u M4XxO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MU[3PFk{Lz5zOUG2O|g6OzxxYU6=
osteosarcoma cells M1rDdmZ2dmO2aX;uJIF{e2G7 MX;BZ5Rqfmm2eTDheEBXTFJiaX6gdoF1KG:|dHXvd4Fz[2:vYTDj[YxteyCjc4Pld5Nm\CCjczCyOE1pgWS{b4j5cIF{\SC2cnHud4NzcXC2aX;uJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwNFIh|ryPLh?= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl4MEmyOEc,OTd7NkC5NlQ9N2F-
ROS 17/2.8 MkHnSpVv[3Srb36gZZN{[Xl? NUfoWo02OTZiaILz MWfBZ5Rqfmm2eTDheEBz[XRicnXjc41jcW6jboSg[pVtdCCuZX7neIghXkSUIHX4dJJme3OnZDDpckBz[XRiUl;TJFE4NzJwODDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKDJ2LXj5[JJwgHmuYYPlJIdmdmVicILvcY91\XJiYYPz[ZN{\WRiYYOgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gZYZ1\XJiMU[gbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODByMjFOwG0v NETjSGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEeyNlE{OCd-MUi3NlIyOzB:L3G+
ROS 17/2.8 NYS3TmtSTnWwY4Tpc44h[XO|YYm= NEfDOGkyPiCqcoO= MXXBZ5Rqfmm2eTDheEBz[XRicnXjc41jcW6jboSg[pVtdCCuZX7neIghXkSUIHX4dJJme3OnZDDpckBz[XRiUl;TJFE4NzJwODDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKDJ2LXj5[JJwgHmuYYPlJIdmdmVicILvcY91\XJiYYPz[ZN{\WRiYYOgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gZYZ1\XJiMU[gbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhU2liPTCwMlAxODJizszNMi=> NXy5UYVJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNFU3PzdpPkKxNVA2Pjd5PD;hQi=>
ROS 17/2.8 M2WzWmZ2dmO2aX;uJIF{e2G7 Ml\pNVYhcHK| NYrlcXZWSWexbnnzeEBi[3Srdnn0fUBifCC{YYSgWmRTKGmwIGLPV{AyPy9{LkigZ4VtdHNidILhcpNn\WO2ZXSge4l1cCCFeYCyOIEyKHKncH;yeIVzKHCuYYPtbYQh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gR5lxOjSjMTD0doFve2O{aYD0bY9vKGGodHXyJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwNFIh|ryPLh?= MmLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMUi5NVgoRjJ{MEG4PVE5RC:jPh?=
osteosarcoma cells NGLEfpJHfW6ldHnvckBie3OjeR?= MUnJcoR2[3Srb36gc4YhXkSULX3l[IlifGWmIEK0MYh6\HKxeInsZZNmKHS{YX7zZ5JqeHSrb36gbY4hemG2IH;zeIVwe2G{Y3;tZUBk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXEOVAhRSByLkCwNFIh|ryPLh?= NHG0ZXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmwNlI{PSd-MkG5NFIzOzV:L3G+
osteosarcoma cells M4\mcWZ2dmO2aX;uJIF{e2G7 NYDtZnpyXHKjboPhZ5RqfmG2aX;uJI9nKF[GUjDpckBz[XRib4P0[Y9{[XKlb33hJINmdGy|IHHzd4V{e2WmIHHzJFI1NWi7ZILvfJlt[XOnIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWGNUCgQUAxNjByMEKg{txONg>? NYe4RnRTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2N|A1PDRpPkK2OlMxPDR2PD;hQi=>
osteosarcoma cells NITyNIVHfW6ldHnvckBie3OjeR?= NGLuWXoyPiCqcoO= Ml7QTY5pcWKrdHnvckBw\iCYRGKtbY5lfWOnZDCyOE1pgWS{b4j5cIF{\SC2cnHud4NzcXC2aX;uJIlvKHKjdDDvd5Rmd3OjcnPvcYEh[2WubIOgZYZ1\XJiMU[gbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUR3MDC9JFAvODByMjFOwG0v M1zKZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEmwNFExLz5{MkS5NFAyODxxYU6=
osteosarcoma cells MVrGeY5kfGmxbjDhd5NigQ>? NIi3W2RC[3Srdnn0fUBifCCYRGKgbY4hemG2IH;zeIVwe2G{Y3;tZUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiMkStbJllem:6eXzhd4UhemWyb4L0[ZIh\2WwZTD0doFve2O{aYD0bY9vKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODByMzFOwG0v NWXqdIxiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4NVk4ODJpPkG5PFE6PzB{PD;hQi=>
HuH7 NFn1OHBHfW6ldHnvckBie3OjeR?= M2Sz[lI1KGi{cx?= MVzB[49vcXO2IHHjeIl3cXS7IHH0JHZFWiCneIDy[ZN{\WRiaX6gbJVu[W5iSIXIO{Bk\WyuczDh[pRmeiB{NDDodpMh[nlicFTSN{1tfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwNFMh|ryPLh?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF2NUC3NUc,OjdzNEWwO|E9N2F-
ROS 17/2.8 MnPnSpVv[3Srb36gZZN{[Xl? M4TiT3ZqfGGvaX6gSFMhemWlZYD0c5IudWWmaXH0[YQhfHKjboPjdolxfGmxbnHsJJBwfGWwY4mgbY4hemG2IH;zeIVwe2G{Y3;tZUBTV1NiMUevNk45KGOnbHzzMEBGTDVyIE2gNE4xODB|NTFOwG0v NWLKV2pGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC0O|kzPzZpPkGwOFc6Ojd4PD;hQi=>
ROS 17/2.8 NID0V3BHfW6ldHnvckBie3OjeR?= Mm\3TY4hfmm2cn:geol1[W2rbjDEJJJm[2WydH;yJEhXTFJrLX3l[IlifGWmIITyZY5{[3KrcITpc45idCCyb4TlcoN6KGmwIILheEBwe3Snb4PhdoNwdWFiUl;TJFE4NzJwODDj[YxteyxiRVS1NEA:KDBwMECwN|Yh|ryPLh?= M1zYXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMECwNFE1Lz5zMUCwNFAyPDxxYU6=
ROS 17/2.8 M334OGZ2dmO2aX;uJIF{e2G7 MmXxTY4hfmm2cn:geol1[W2rbjDEJJJm[2WydH;yMY1m\GmjdHXkJIFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIlvKHKjdDDvd5Rmd3OjcnPvcYEhWk:VIEG3M|IvQCClZXzsd{whTUR3MDC9JFAvODByNDFOwG0v NVrTflViRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5N|E3OjdpPkGxPVMyPjJ5PD;hQi=>
COS7 M1fDSWZ2dmO2aX;uJIF{e2G7 NGX4bHpKdmS3Y4Tpc44hd2ZidILhcpNi[3SrdnH0bY9vKG:oIHj1cYFvKF[GUjDy[ZNxd26|aY\lJIdmdmViaX6gR29UPyClZXzsd{BjgSC{YYSgc5N1\W:yb370bY4hdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wNFA2KM7:TT6= NWfXPYFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUeyPVgxPDVpPkG3Nlk5ODR3PD;hQi=>
COS7 M{\rSmZ2dmO2aX;uJIF{e2G7 M{PST|I1KGi{cx?= M17kbGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gWmRTKGmwIFPPV|ch[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIITyZY5{[3KrcITpc45idCC2cnHud4FkfGm4YYTpc44h[W[2ZYKgNlQhcHK|IHL5JI1wfXOnIH;zeIVweG:wdHnuJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMECwOUDPxE1w MoPCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7MESzO|AoRjF5OUC0N|cxRC:jPh?=
SVEC M4n6c2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NWK2W3p{PzJiaILz MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKFOYRVOgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h[2WubDDueY1j\XJiYX\0[ZIhPzJiaILzJIJ6KFeVVDDhd5NigSxiSVO1NEA:KDBwMECwOVIh|ryPLh?= NV3mU245RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA{OzlpPkK0PVAxOzN7PD;hQi=>
osteosarcoma cells MnrkSpVv[3Srb36gZZN{[Xl? NXjDVlQxTW[oZXP0JI9vKF[GUjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliaX6gbJVu[W5ib4P0[Y9{[XKlb33hJINmdGy|IHnuJJBz\XOnbnPlJI9nKDVnIH\leIFtKGOjbH[gd4VzfW1uIFXDOVAhRSByLkCwNFU2PSEQvF2u MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB3NEKzNEc,OThyNUSyN|A9N2F-
HEK293 NGD1R5JHfW6ldHnvckBie3OjeR?= M{nSeGFod26rc4SgZYN1cX[rdImgZZQhXkSUIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyClb4TyZY5{\mWldHXkJJdqfGhiUmjSZYxxcGFiYYPz[ZN{\WRiYYOgVnhT[WyyaHGgdoVkenWrdH3lcpQh[nlidIfvMYh6[nKrZDDhd5NigSxiRVO1NEA:KDBwMECwOkDPxE1w MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZzM{SyNEc,OjZ4MUO0NlA9N2F-
CV1 M3TjSmZ2dmO2aX;uJIF{e2G7 M{PKS2FkfGm4aYT5JI9vKGi3bXHuJHZFWi2vZXTpZZRm\CC2cnHud4NzcXC2aX;uJI9nKG:|dHXvZ4Ft[2mwIG\EVmUh\nW|ZXSgeI8hfGi7bXnkbY5mKGurbnHz[UBxem:vb4Tldk9oem:5dHigbI9zdW:wZTDy[ZBwenSncjDn[Y5mKGmwIFPWNUBk\WyuczygSWQ2OCB;IECuNFAxPyEQvF2u MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF2OUi4NEc,OTdzNEm4PFA9N2F-
NHEK M1vaZmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NEPSSmwzPCCqcoO= NEXSToxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JG5JTUtiY3XscJMhcW6ldXLheIVlKG[xcjCyOEBpenNiYomgX|NJZXSqeX3p[Ilv\SCrbnPvdpBwemG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNFA4OjhizszNMi=> NEm1XWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSwOFMzPid-MkK0NFQ{OjZ:L3G+
COS1 MXTGeY5kfGmxbjDhd5NigQ>? MYnEbZNxdGGlZX3lcpQhd2ZiMT3hcJBp[SxiMkWtLG9JMVt|SG3EN{Bnem:vIHj1cYFvKF[GUjDlfJBz\XO|ZXSgbY4hS0:VMTDj[YxteyxiSVO1NEA:KDBwMECwPEDPxE1w M1XuelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUK0OlE3Lz5zN{myOFYyPjxxYU6=
HEK293 MoG5SpVv[3Srb36gZZN{[Xl? M1;J[lI1KGi{cx?= Mlr0RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDWSHIhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjD0doFve2O{aYD0bY9v[WxidILhcpNi[3SrdnH0bY9vKGGodHXyJFI1KGi{czDifUBud3W|ZTDvd5Rmd3CxboTpckBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwNUDPxE1w NVjOTYdsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFQ{PzBpPkG3PVA1OzdyPD;hQi=>
HL60 M4D6bGZ2dmO2aX;uJIF{e2G7 NXKyOHlVUW6mdXP0bY9vKG:oIHj1cYFvKEiONkCg[Iln\mW{ZX70bYF1cW:wIHHzd4V{e2WmIHHzJINmdGy|IILl[JVkcW6pIH7peJJwKGKudXWgeIV1emG8b3zpeY0tKEWGNUCgQUAxNjByMTFOwG0v M3HmXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUCyNlM2Lz5{MUmwNlI{PTxxYU6=
HeLa MVHGeY5kfGmxbjDhd5NigQ>? MoPwNVYhcHK| M3X3O3Rz[W6|YXP0bZZifGmxbjDv[kBISUx2LX\1d4VlKF[GUjDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhOTZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFAyKM7:TT6= NVy0WFIxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5OVc5OzRpPkKyPVU4QDN2PD;hQi=>
T47D NFnKOodCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NHzX[IY4OiCqcoO= NFzTe3lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGS0O2Qh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEBvfW2kZYKgZYZ1\XJiN{KgbJJ{KGK7IGfTWEBie3OjeTygTWM2OCB;IECuNFAyOyEQvF2u MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEOzPUc,OjR7MECzN|k9N2F-
ROS 17/2.8 MoK5SpVv[3Srb36gZZN{[Xl? NGjIUJlC[3Srdnn0fUBifCCqdX3hckBz\WOxbXLpcoFvfCCYRGKgbY4hWk:VIEG3M|IvQCClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJIdzd3e2aDDoc5Jud26nIH\1d4lwdiCpZX7lJHZFWkViYYPz[ZN{\WRiYYOg[5Jwf3SqIHjvdo1wdmVibHX2[Ywh[nlidILhcpNkemmydHnvcoFtKGG|c3H5MEBGTDVyIE2gNE4xODF3IN88UU4> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl{NE[xOkc,OTd7MkS2NVY9N2F-
HEK293 Ml;jSpVv[3Srb36gZZN{[Xl? MXqyOEBpenN? MWfUdoFve2GldHn2ZZRqd25ib3[gdoVkd22kaX7hcpQhcHWvYX6gWmRTN1K[UnHsdIhiKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JGR2[WxvbIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xODF3ODFOwG0v MmjtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUOyNVYoRjNyMUmzNlE3RC:jPh?=
MCF7 NW\GdWN6TnWwY4Tpc44h[XO|YYm= M{HqO|I1KGi{cx?= MYnB[49vcXO2IHHjeIl3cXS7IHH0JHZFWiCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKHS{YX7zZ5JqeHSrb36gc4YhS1mSMkTBNUBo\W6nIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlAxOTZizszNMi=> NFfWS5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUezN|Y1PSd-Mkm3N|M3PDV:L3G+
MCF10DCIS.com NIn6O49CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MljnN{Bl[Xm| MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFXSJJBwe2m2aY\lJIh2dWGwIF3DSlExTEOLUz7jc40h[2WubIOgZYZ1\XJiMzDkZZl{KGK7IGuzTH11cHmvaXTpcoUhfXC2YXvlJIF{e2G7LDDJR|UxKD1iMD6wNFE4PSEQvF2u MkPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzOEC4N|coRjJ{MUiwPFM4RC:jPh?=
HL60 NXzCfFVnTnWwY4Tpc44h[XO|YYm= M{T3dmlv\HWldHnvckBw\iCmaX\m[ZJmdnSrb36gc4YhcHWvYX6gTGw3OCClZXzsd{BqdnSxIH3vco9kgXSnczDifUBPSlRicnXkeYN1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMEKg{txONg>? NX36dGhWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5OlA6OjRpPkG3PVYxQTJ2PD;hQi=>
HL-60 NF\yTHRHfW6ldHnvckBie3OjeR?= MmjJOEBl[Xm| NUmzZY5TUW6mdXP0bY9vKG:oIHTp[oZmemWwdHnheIlwdiCxZjDoeY1idiCKTD22NEBk\WyuczDwdo9ugWWub3P5eIV{KHSxIH3vco9kgXSnczDh[pRmeiB2IHThfZMh[nliTlLUJJJm\HWldHnvckBie3OjeTygSWM2OCB;IECuNFAzKM7:TT6= NE\oN4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEeyNlE{OCd-MUi3NlIyOzB:L3G+
bone marrow cells NELJemRHfW6ldHnvckBie3OjeR?= NI[1Sm4{KGSjeYO= NFXGZXRG\m[nY4Sgc44hd3O2ZX;jcIF{fCCob4LtZZRqd25iaX6g[IR[KG2xdYPlJIJwdmVibXHydo94KGOnbHzzJINwKGO3bIT1doVlKHerdHigdJJqdWG{eTDvd5Rmd2OuYYP0d{Bi\nSncjCzJIRigXNuIFXDOVAhRSByLkCwNkDPxE1w M2DzbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUO5NFM1Lz5zOEWzPVA{PDxxYU6=
HL60 MU\GeY5kfGmxbjDhd5NigQ>? MV[0JIRigXN? M4nDfGlv\HWldHnvckBw\iCmaX\m[ZJmdnSrYYTpc44hd2ZiaIXtZY4hUEx4MDDj[YxteyCycn;tfYVtd2O7dHXzJJRwKG2xbn;jfZRmeyCjZoTldkA1KGSjeYOgZpkhVkKWIILl[JVkfGmxbjDhd5NigSxiS3mgQUAxNjByMjFOwG0v NUDyWYhNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNFU3PzdpPkKxNVA2Pjd5PD;hQi=>
HeLa Ml\BSpVv[3Srb36gZZN{[Xl? NHize24yQCCqcoO= M3[3S3Rz[W6|YXP0bZZifGmxbjDv[kBnfWyuIHzlcod1cCCqdX3hckBXTFJiZYjwdoV{e2WmIHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iQ2nQNlQhfHKjboPjdolxfGmxbjDh[pRmeiBzODDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xODJ3IN88UU4> NWntSJRGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5OVc5OzRpPkKyPVU4QDN2PD;hQi=>
PBMC MkLQSpVv[3Srb36gZZN{[Xl? Ml7rTY4hfmm2cn:gbY5pcWKrdHnvckBw\iCyaIn0c4hmdWGpZ3z1eIlvcW6rbj3pcoR2[2WmIIP0bY12dGG2aX;uJI9nKGi3bXHuJJBmemmyaHXyZYwh[myxb3SgcY9vd263Y3zlZZIh[2WubIOgdJJwdGmoZYLheIlwdixiRVS1NEA:KDBwMECyO{DPxE1w MnfxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTFyMECwNVQoRjFzMECwNFE1RC:jPh?=
HL60 M4HSPWZ2dmO2aX;uJIF{e2G7 M4O3dGlv\HWldHnvckBw\iClZXzsJIRq\m[ncnXueIlifGmxbjDv[kBpfW2jbjDIUFYxKGOnbHzzJJRwKG2xbn;jfZRmeyCkeTDORnQhemWmdXP0bY9vKGG|c3H5MEBGSzVyIE2gNE4xODNizszNMi=> NWG3[5NtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxOlc5QTNpPkG5NVY4QDl|PD;hQi=>
HEK293 M2rnbGZ2dmO2aX;uJIF{e2G7 MXPB[49vcXO2IHHjeIl3cXS7IHH0JGNOYC2JQVy0JJRi\2enZDDoeY1idiCYRGKg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xODNizszNMi=> NF24NYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S2NlcyPSd-MkO0OlI4OTV:L3G+
MCF7 M{jFTmZ2dmO2aX;uJIF{e2G7 NYHTRZk1OjRiaILz M3X3XmFod26rc4SgZYN1cX[rdImgZZQhXkSUIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO|ZXSgZZMhfHKjboPjdolxfGmxbjDv[kBEYVB{NFGxJIdmdmViYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFA{OjhizszNMi=> Mn7PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OEmzO|koRjJ{OUi5N|c6RC:jPh?=
Caco-2 MlTFSpVv[3Srb36gZZN{[Xl? M4\GdWVn\mWldDDvckBXTFJidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGmwIHj1cYFvKEOjY3:tNkBk\WyuczDpckBxemW|ZX7j[UBw\iB3JTDm[ZRidCClYXzmJJNmenWvLDDFR|UxKD1iMD6wNFM2OiEQvF2u Mm\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNUSyN|AoRjF6MEW0NlMxRC:jPh?=
MCF10AT1 M{XMOmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NHT1eZA{KGSjeYO= MojFRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFVkBxd3OrdHn2[UBpfW2jbjDNR2YyOEGWMTDj[YxteyCjZoTldkA{KGSjeYOgZpkhYzOKXYTofY1q\GmwZTD1dJRic2ViYYPzZZktKEmFNUCgQUAxNjByM{mg{txONg>? M2PBcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUiwPFM4Lz5{MkG4NFg{PzxxYU6=
Jurkat NFHJRZJHfW6ldHnvckBie3OjeR?= M4DyeVI1KGi{cx?= MYLBZ5RqfmG2aX;uJI9nKF[GUjDpckBpfW2jbjDKeZJs[XRiY3XscJMh\XiycnXzd4lv\yCuZX70bZZqemGuIG\EVmUudHWlaX\ldoF{\SC4ZXP0c5Ih[XO|ZYPz[YQh[XNiVlTSSU1u\WSrYYTl[EB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgeJJidnOlcnnweIlwdmGuIILldI9zfGW{IHHzd4F6NCCHQ{WwJF0hOC5yMEO5N|Yh|ryPLh?= M4rRTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEewO|c6Lz5{N{C3NFc4QTxxYU6=
MCF7 MWPGeY5kfGmxbjDhd5NigQ>? NF;GV2ZC\2:waYP0JIFkfGm4aYT5JIF1KHqnYoLh[ol{cCCpYXy0MXZFWiCOQlSg[ZhxemW|c3XkJIlvKGi3bXHuJG1ETjdiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGKjc3XkJJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEC1OUDPxE1w NWSxPZlrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxPFA5OzdpPkKyNVgxQDN5PD;hQi=>
MCF10CA1a M13udGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M3u4VlMh\GG7cx?= NV3COJZOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGWiCyb4PpeIl3\SCqdX3hckBOS0ZzMFPBNYEh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IGuzTH11cHmvaXTpcoUhfXC2YXvlJIF{e2G7LDDJR|UxKD1iMD6wNFYzKM7:TT6= NYqzVWo{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxPFA5OzdpPkKyNVgxQDN5PD;hQi=>
HL60 NWrDTHUyTnWwY4Tpc44h[XO|YYm= NXjaVHQyQTZiaILz MnHuRYdwdmm|dDDhZ5Rqfmm2eTDheEBXTFJiaX6gbJVu[W5iSFy2NEBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiY3XscEBlcW[oZYLlcpRq[XSrb36gZYZ1\XJiOU[gbJJ{KGK7IF7CWEBie3OjeTygSWM2OCB;IECuNFA6KM7:TT6= NHyzd2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWxPFMyQSd-Mkm1NVg{OTl:L3G+
HL-60 NFrMRVlHfW6ldHnvckBie3OjeR?= M3\HeVk3KGi{cx?= MnyxRYdwdmm|dDDhZ5Rqfmm2eTDheEB3cXSjbXnuJGQ{KHKnY3XweI9zKGmwIHj1cYFvKEiOLU[wJINmdGy|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iClZXzsJIRq\m[ncnXueIlifGmxbjDh[pRmeiB7NjDodpMh[nliTlLUJIR6\S2kYYPl[EBucWO{b4Pjc5Bq[yCjbnHsfZNqeyxiRVO1NEA:KDBwMEC5JO69VS5? NHLsfHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2NlU1Oid-Mk[1OlI2PDJ:L3G+
THP1 M{D1SmZ2dmO2aX;uJIF{e2G7 NVXxU4UxSWO2aY\heIlwdiCxZjDWSHIhcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckAzPS2qeXTyc5h6fmm2YX3pckBFNTJ2LXj5[JJwgHmuYYPlJI1TVkFiZYjwdoV{e2mxbjDifUBTXC2SQ2KsJGVEPTBiPTCwMlAyKM7:TT6= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTNyOUG1OUc,OTl|MEmxOVU9N2F-
THP1 MoDhSpVv[3Srb36gZZN{[Xl? M2[5W2FkfGm4YYTpc44hd2ZiVlTSJIlvKGi3bXHuJHRJWDFiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZ4F1cGWuaXPp[IlvKGGwdHntbYNzd2KrYXygdIVxfGmmZTDtVm5CKGW6cILld5Nqd25iYomgVnQuWEOULDDFR|UxKD1iMD6wNUDPxE1w MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTNyOUG1OUc,OTl|MEmxOVU9N2F-
ROS 17/2.8 M1LER2Z2dmO2aX;uJIF{e2G7 M{LQXGVn\mWldHn2[UBld3OnIH\vdkB1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBqdiCUT2OgNVcwOi56IH;zeIVwe2G{Y3;tZUBk\WyuczygSWQ2OCB;IECuNFEh|ryPLh?= MlTFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB6OUOzNFkoRjFyOEmzN|A6RC:jPh?=
HL60 M12ycGZ2dmO2aX;uJIF{e2G7 NI\oflAyPDRiaILz NE\SO|FKdmS3Y4Tpc44hd2ZiY3XscEBlcW[oZYLlcpRq[XSrb36gc4YhcHWvYX6gTGw3OCClZXzsd{BqdmO3YnH0[YQh\m:{IEG0OEBpenNiYomgW5N1NTFiZInlJIJie2WmIHHzd4F6NCCLQ{WwJF0hOC5yNTFOwG0v MoTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzOEC5NlYoRjJ3MUiwPVI3RC:jPh?=
MCF7 NYrONHRrSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NFjNS|c4OiCqcoO= M33zSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliW{PIYZRpgW2rZHnu[UBqdmOxcoDvdoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yNUKg{txONg>? NX[zTm9YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3N|k3PzJpPkG5O|M6Pjd{PD;hQi=>
bone marrow cells MWPGeY5kfGmxbjDhd5NigQ>? NYPqTGtOOyCmYYnz MYPF[oZm[3Rib36gc5N1\W:lbHHzeEBnd3KvYYTpc44hcW5iZHTZJI1wfXOnIHLvcoUhdWG{cn;3JINmdGy|IHPvJIN2dHS3cnXkJJdqfGhicILpcYFzgSCxc4Tlc4Nt[XO2czDh[pRmeiB|IHThfZMhemWuYYTpeoUhfG9iMT3hcJBp[SxiMkWtLG9JMTKGMzygSWM2OCB;IECuNUDPxE1w M1\XWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUO5NFM1Lz5zOEWzPVA{PDxxYU6=
HL-60 M3XWPGZ2dmO2aX;uJIF{e2G7 M2rjemlvcGmkaYTpc44hd2ZiY3;sc456KG[xcn3heIlwdiCjc4PhfZMhd25iSFytOlAh[2WubIOsJGVFPTBiPTCwMlE{KM7:TT6= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODh7M{OwPUc,OTB6OUOzNFk9N2F-
Caco2 NFLWelFHfW6ldHnvckBie3OjeR?= M1XuPFI1KGi{cx?= M1r4[2FkfGm4YYTpc44hd2ZiVlTSJIlvKGi3bXHuJGNi[29{IHPlcIx{KGW6cILld5NqdmdibHXueIl3cXKjbDDWSHJGNWy3Y3nm[ZJie2VidnXjeI9zKGG|c3Xzd4VlKGG|IG\EVmUudWWmaXH0[YQhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JI1m[XO3cnXkJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJRz[W6|Y4LpdJRqd26jbDDy[ZBwenSncjDhd5NigSxiRVO1NEA:KDBwMkKzOEDPxE1w NIPTUnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C3NFc4QSd-MkewO|A4Pzl:L3G+
HaCaT M3ixbGZ2dmO2aX;uJIF{e2G7 NH\hNVUzPCCqcoO= MnLtRYN1cX[jdHnvckBw\iCYRGKgbY4hcHWvYX6gTIFE[VRiY3XscJMh\XiycnXzd4lv\yCuZX70bZZqemGuIG\EVmUudHWlaX\ldoF{\SC4ZXP0c5Ih[XO|ZYPz[YQh[XNiVlTSSU1u\WSrYYTl[EB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgeJJidnOlcnnweIlwdmGuIILldI9zfGW{IHHzd4F6NCCHQ{WwJF0hOC5{NEm3JO69VS5? NF7JRnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C3NFc4QSd-MkewO|A4Pzl:L3G+
HaCaT M2P2NmZ2dmO2aX;uJIF{e2G7 MW[yOEBpenN? M2\0c3Rz[W6|YXP0bZZifGmxbjDv[kBXTFJiaX6gbJVu[W5iSHHDZXQh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOzJzNTFOwG0v MknaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|NkewNVkoRjJ4M{[3NFE6RC:jPh?=
MCF-7 M2[4e2Z2dmO2aX;uJIF{e2G7 MXHJcohq[mm2aX;uJI9nKGOxbH;ufUBnd3KvYYTpc44h[XO|YYnzJI9vKE2FRj23JINmdGy|LDDFSFUxKD1iMD6zPEDPxE1w NWHEPVZbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC4PVM{ODlpPkGwPFk{OzB7PD;hQi=>
CV1 NX3wdnRZTnWwY4Tpc44h[XO|YYm= NXfpZppPTW[oZXP0JI9vKF[GUj3t[YRq[XSnZDD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliaX6gR3YyKGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigbJVu[W5icnXjc41jcW6jboSgWmRTNCCHREWwJF0hOC52IN88UU4> NVP1TWZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW2NVU2OzRpPkG1OlE2PTN2PD;hQi=>
Caco2 NF;uWHBHfW6ldHnvckBie3OjeR?= MViyOEBpenN? NUfRNHVIXHKjboPhZ5RqfmG2aX;uJI9nKF[GUjDpckBpfW2jbjDDZYNwOiClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC53MUWyJO69VS5? MlTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|NkewNVkoRjJ4M{[3NFE6RC:jPh?=
HN12 M3zaXmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NEi3fJE4OiCqcoO= M3\aOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF6xNkBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiClZXzsJI52dWKncjDh[pRmeiB5MjDodpMh[nliV2PUJIF{e2G7LDDJR|UxKD1iMD61OVMh|ryPLh?= NGDocGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFM{QSd-MkS5NFA{Ozl:L3G+
L02 Ml:0RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? Mkf6OFghcHK| NILlPIdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFywNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD62O{DPxE1w M{fMdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUG4N|E6Lz5{OUWxPFMyQTxxYU6=
L02 NF7V[ppEgXSxdH;4bYNqfHliYYPzZZk> MmH1OFghcHK| MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMNFIh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuOlch|ryPLh?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV4MkW0Nkc,OjZ3NkK1OFI9N2F-
Caco2 NEXOPHNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mo[3OFghcHK| NIHMTJhCdnSrcH;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ3Hjc|Ih[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IESuOEDPxE1w MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV4MkW0Nkc,OjZ3NkK1OFI9N2F-
Caco2 NGnp[HRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NE[zOpU1QCCqcoO= MlnCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCFYXPvNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iND60OkDPxE1w NWDJfFZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NVg{OTlpPkK5OVE5OzF7PD;hQi=>
MCF7 M2\MWmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGi4eIg1QCCqcoO= NWW4O3ZxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC1MlU6KM7:TT6= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTVzOEOxPUc,Ojl3MUizNVk9N2F-
MCF7 MUTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MWC0PEBpenN? MWrBcpRqeG:uaX\ldoF1cX[nIHHjeIl3cXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPS54IN88UU4> NGf0SlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2NlU1Oid-Mk[1OlI2PDJ:L3G+
MCF7 Ml\kSpVv[3Srb36gZZN{[Xl? M2jhR|E3KGi{cx?= MVfCbY5lcW6pIHHm[olvcXS7IITvJHZFWiCrbjDzZ5JidWKuZXSgd4lTVkFvdILhcpNn\WO2ZXSgbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIHL5JI1m[XO3cnnu[{Bz\WS3Y4Tpc44hcW5iQoLkWUBqdmOxcoDvdoF1cW:wIHHmeIVzKDF4IHjyd{BjgSCHTFnTRUwhUUN3MDC9JFYvPDNizszNMi=> M2fDXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUmzNlE3Lz5|MEG5N|IyPjxxYU6=
PC3 NFn0RVFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NX7FR5Z{OjRiaILz Mn3WRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCSQ{OgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDlwNUOg{txONg>? NYq5R|E1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVMzOTZpPkOwNVk{OjF4PD;hQi=>
MCF7 M{HXbmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnPFNlQhcHK| MofHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzMT60N{DPxE1w MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7M{KxOkc,OzBzOUOyNVY9N2F-
MCF7 NH3UV|FCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M{Tad|czKGi{cx?= MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCycn;sbYZmemG2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxNk42KM7:TT6= NX7KbFBURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlcyPDlpPkK1NVI4OTR7PD;hQi=>
PC3 NFnuXGVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M{\LVFQ5KGi{cx?= NF;pTXFCdnSrcH;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOzJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzNz6yJO69VS5? NIPqXIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2NlU1Oid-Mk[1OlI2PDJ:L3G+
PC3 MXLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M3izOlQ5KGi{cx?= M3fIWGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOzJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzNz6yOUDPxE1w NEPjSlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWxPFMyQSd-Mkm1NVg{OTl:L3G+
BxPC3 NFLoPIpCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NITpcWwzPCCqcoO= NHP4dldCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFL4VGM{KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCyNU4xPyEQvF2u NInWNJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEG5N|IyPid-M{CxPVMzOTZ:L3G+
PBMC M3LZbmZ2dmO2aX;uJIF{e2G7 NFrCW5gyOCCwTR?= NE\z[I92eCC2bzC4JIhzew>? NELjWYJC[3SrdnH0bY9vKG:oIG\EVkBqdiCOUGOtd5RqdXWuYYTl[EBpfW2jbjDQRm1EKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIEK1MYh6\HKxeIn2bZRidWmwIFStNlQucHmmcn;4fYxie2VibWLORUBmgHC{ZYPzbY9vKGG2IEGwJI5OKGGodHXyJJVxfG9iODDodpMh[nliUmStVGNT MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTNyOUG1OUc,OTl|MEmxOVU9N2F-
THP1 NU\MU4wzTnWwY4Tpc44h[XO|YYm= M3nkb2FkfGm4YYTpc44hd2ZiVlTSJIlvKGi3bXHuJHRJWDFiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZ4F1cGWuaXPp[IlvKGGwdHntbYNzd2KrYXygdIVxfGmmZTDtVm5CKGW6cILld5Nqd25iYYSgSWM2OCC3cITvJFUhcHK|IHL5JHJVNVCFUh?= NUTDTnNjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzNFkyPTVpPkG5N|A6OTV3PD;hQi=>
NB4 MknpSpVv[3Srb36gZZN{[Xl? NUXyc4VSOSCwTR?= NFXRUGM1QCCqcoO= NEjVOWlKdmS3Y4Tpc44hd2ZiY3XscEBlcW[oZYLlcpRq[XSrb36gbY4hcHWvYX6gUmI1KGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDpcoR2[3Srb36gc4Yh[2WubDDzeZJn[WOnIH3hdotmeiCFREG0JIV5eHKnc4Ppc44h[XRiMTDuUUBi\nSncjC0PEBpenN? M3LTWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7Nki1PFg5Lz5zOU[4OVg5QDxxYU6=
NB4 M1m0bmZ2dmO2aX;uJIF{e2G7 MXmxJI5O MoXvOFghcHK| MXLJcoR2[3Srb36gc4Yh[2WubDDkbYZn\XKnboTpZZRqd25iaX6gbJVu[W5iTlK0JINmdGy|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iCrbnT1Z5Rqd25ib3[gZ4VtdCC|dYLmZYNmKG2jcnvldkBETDFzYjDlfJBz\XO|aX;uJIF1KDFibl2gZYZ1\XJiNEigbJJ{ NH\zO2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[4OVg5QCd-MUm2PFU5QDh:L3G+
HL60 MULGeY5kfGmxbjDhd5NigQ>? MmS4OEBl[Xm| NHfDfG5KdmS3Y4Tpc44hd2ZiY3XscEBlcW[oZYLlcpRq[XSrb36gbY4hcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC0JIRigXNiYomgUmJVKHKnZIXjeIlwdiCjc4PhfS=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF7M{C1PUc,OTlzOUOwOVk9N2F-
HL60 NGfpOmlHfW6ldHnvckBie3OjeR?= M3ezZ|ExKM7:TR?= M324dWlvcGmkaYTpc44hd2ZiMTyyOU0pV0hrMlSzMYlv\HWlZXSgZ4VtdCCmaX\m[ZJmdnSrYYTpc44hcW5iaIXtZY4hUEx4MDDj[YxteyCjc4Pld5Nm\CCjczDtc5JxcG:ub3fpZ4FtKGOqYX7n[ZMh[XRiMUCnMVYhVSCkeTDXdolocHRvR3XpcZNiKHO2YXnubY5o M1fzelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUmzNFU6Lz5zOUG5N|A2QTxxYU6=
SCC4 M{LKUWZ2dmO2aX;uJIF{e2G7 MW[xJJVO M3ztZlUhfG9iMUCgcYlvew>? NXLhVHFISWexbnnzeEBi[3Srdnn0fUBifCCYRGKgbY4hcHWvYX6gV2NEPCClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gR3lROjRiZ3Xu[UBmgHC{ZYPzbY9vKGG2IEGgeW0h[W[2ZYKgOUB1dyBzMDDtbY5{KGK7IGLUMXBEWiCjbnHsfZNqew>? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MkKyOUc,OjB2NUKyNlU9N2F-
SCC25 NV;5bIFtTnWwY4Tpc44h[XO|YYm= MoP6NVAhdk1? MoD4RYdwdmm|dDDhZ5Rqfmm2eTDheEBXTFJiaX6gbJVu[W5iU1PDNlUh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIFPZVFI1KGW6cILld5Nqd25iYYSgNVAhdk1? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh6M{CyOkc,OjB6OEOwNlY9N2F-
SCC25 NXPofIVtTnWwY4Tpc44h[XO|YYm= NG\NfWYyODBibl2= MkHrRYdwdmm|dDDhZ5Rqfmm2eTDheEBXTFJiaX6gbJVu[W5iU1PDNlUh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIGTTUHAh\XiycnXzd4lwdiCjdDCxNFAhdk1? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh6M{CyOkc,OjB6OEOwNlY9N2F-
C3H10T1/2 MkDvSpVv[3Srb36gZZN{[Xl? NFvoT4ExNjVidH:gOUB2VQ>? MXuyOEBpenN? M4XiWmlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IHTve44hemWpdXzheIlwdiCxZjDHcIkyKGG2IECuOUB1dyB3IIXNJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? M{L2RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkK2OlU4Lz5{MkKyOlY2PzxxYU6=
HL60 NX3YXFJrTnWwY4Tpc44h[XO|YYm= M1fGfVEh|ryP MWnJcoR2[3Srb36gc4Yhfmm2YX3pckBFKHKnY3XweI9zNW2nZHnheIVlKGSrZn\ldoVvfGmjdHnvckBw\iCqdX3hckBJVDZyIHPlcIx{KGG2IEGwK{02KE1iYomgUmJVKHKnZIXjeIlwdiCvZYToc4Q> NHPPO4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK4OVk1Oyd-MkKyPFU6PDN:L3G+
HL60 MYPGeY5kfGmxbjDhd5NigQ>? MmPtNUBvVSC2bzCxNEDPxE1? NF2yNGhKdmS3Y4Tpc44hd2Zidnn0ZY1qdiCGIILlZ4VxfG:{LX3l[IlifGWmIHTp[oZmemWwdHnheIlwdiCxZjDoeY1idiCKTE[wJINmdGy|IHH0JFExLy17IITvJFExLy13IF2gZpkhVkKWIILl[JVkfGmxbjDt[ZRpd2R? M4qwcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mki1PVQ{Lz5{MkK4OVk1OzxxYU6=
A498 MmLaSpVv[3Srb36gZZN{[Xl? Ml\SNVAhdk1? NFzI[Yw1KGi{cx?= Ml;JTY5lfWO2aX;uJI9nKEO\UEK0JI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDBOFk5KGOnbHzzJIF1KDFyJz24JG0h[W[2ZYKgOEBpenNiYomgVnQuWEOUIHHuZYx6e2m| NV7RcXF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzPVI6QDNpPkKxN|kzQTh|PD;hQi=>
A498 M33BT2Z2dmO2aX;uJIF{e2G7 MnnyNVAhdk1? M4XM[lE3KGi{cx?= NXXLbI1IUW6mdXP0bY9vKG:oIFPZVFI1KG2UTlGg[ZhxemW|c3nvckBqdiCqdX3hckBCPDl6IHPlcIx{KGG2IEGwK{05KE1iYX\0[ZIhOTZiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=> NYjlUmt2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzPVI6QDNpPkKxN|kzQTh|PD;hQi=>
DU145 NUjGT2ZITnWwY4Tpc44h[XO|YYm= MYq3MlUhfU1? NWXhcYo4OThiaILz MkC1RYdwdmm|dDDhZ5Rqfmm2eTDheEBXTFJvTFLEJIlvKGi3bXHuJGRWOTR3IHPlcIx{KGG|c3Xzd4VlKGG|IFPZVFI1STFiZYjwdoV{e2mxbjDheEA4NjVidV2gZYZ1\XJiMUigbJJ{KGK7IIHSWE1RS1JibXX0bI9l M{i1cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{e0PVI6Lz5{Nke3OFkzQTxxYU6=
HaCaT M3Hwc2Z2dmO2aX;uJIF{e2G7 M{O5N2lvcGmkaYTpc44hd2ZiY3;sc456KG[xcn3heIlwdiCxZjDoeY1idiCKYVPhWEBk\Wyucx?= M{XTV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEC0N|I3Lz5{MkSwOFMzPjxxYU6=
HCT116 M4P3Z2Z2dmO2aX;uJIF{e2G7 NWr0eWxVOjBidH:gOFAhfU1? MVOyOEBpenN? NF\SS5lC\2:waYP0JIFkfGm4aYT5JIF1KFKDUjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBpfW2jbjDIR3QyOTZiY3XscJMh[29vZYjwdoV{e2mwZzDSRXJGKGG2IEKwJJRwKDRyIIXNJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> MmnjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{N{[0OFkoRjJ|Mke2OFQ6RC:jPh?=
HCT116 NFXoepZHfW6ldHnvckBie3OjeR?= MXWyNEB1dyB2MDD1US=> MnTCNlQhcHK| NEOyOJpC\2:waYP0JIFkfGm4aYT5JIF1KFK[UjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBpfW2jbjDIR3QyOTZiY3XscJMh[29vZYjwdoV{e2mwZzDSXHJGKGG2IEKwJJRwKDRyIIXNJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> M2eyS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Mke2OFQ6Lz5{M{K3OlQ1QTxxYU6=
HEK293 MkXsSpVv[3Srb36gZZN{[Xl? MWexNFAhdk1? M3XGN|I1KGi{cx?= M1fsU2lv\HWldHnvckBw\iCleYCyOGEyKG2UTlGg[ZhxemW|c3nvckBqdiCKRVuyPVMh[2WubIOgZZQhOTByIH7NJIFnfGW{IEK0JIhzeyCkeTDy[YFtNXSrbXWgVGNT NIXSPW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe3N|U3PSd-MkS3O|M2PjV:L3G+
HEK293 NUjXbGtsTnWwY4Tpc44h[XO|YYm= Mor1NVAhdk1? NXTJ[HVYXHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHZFWiCGMUS5RUBufXSjboSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDjc4V5eHKnc4PpcochS1mSM1G0JIF1KDFyIH7NJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 MoPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUi4OVcoRjJ2OEG4PFU4RC:jPh?=
HEK293 NVjYUoRMTnWwY4Tpc44h[XO|YYm= NUG3flJCOTBibl2= M1K1enRz[W6|YXP0bZZifGmxbjDv[kBpfW2jbjDWSHIhUzJ2MFGgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiY3;lfJBz\XO|aX7nJGN[WDODNDDheEAyOCCwTTDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> NFrCVYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixPFg2Pyd-MkS4NVg5PTd:L3G+
MCF7 NWDTb5o4SW62aYT1cY9zKGG|c3H5 MnLjNE42KG2pL3vn MoDvN{B4\WWtcx?= NH;pUXRCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJJhmdm:pcnHmeIVlKGmwIFLhcIIw[yCwdXTlJI1wfXOnIHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKHB{MTDlfJBz\XO|aX;uJIF1KDBwNTDt[{9s\yxiaYCgZYRucW6rc4TldoVlKHSqcnXlJJRqdWW|IHGge4VmcyCob4KgeIhz\WVid3Xlb5Mhe3SjcoTpcoch\nKxbTDkZZkhPyCjZoTldkB1fW2xcjDj[YxtKGmwb3P1cIF1cW:wIHL5JIludXWwb3jpd5Rw[2inbXnjZYwh[W5? NYDoRYJ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVMzOTZpPkOwNVk{OjF4PD;hQi=>
MCF7 MV7BcpRqfHWvb4KgZZN{[Xl? MVywMlUhdWdxa3e= NFfFR4g{KHenZXvz M2OwVmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgfIVvd2e{YX\0[YQhcW5iQnHsZk9kKG63ZHWgcY92e2ViYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4heDJ5IHX4dJJme3Orb36gZZQhOC53IH3nM4toNCCrcDDh[I1qdmm|dHXy[YQhfGi{ZXWgeIlu\XNiYTD3[YVsKG[xcjD0bJJm\SC5ZXXrd{B{fGG{dHnu[{Bnem:vIHThfUA4KGGodHXyJJR2dW:{IHPlcIwhcW6xY4XsZZRqd25iYomgbY1ufW6xaHnzeI9kcGWvaXPhcEBidg>? NVPYTHNtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVMzOTZpPkOwNVk{OjF4PD;hQi=>
MCF7 M1nVdmFvfGm2dX3vdkBie3OjeR?= MV[wMlUhdWdxa3e= Ml6zN{B4\WWtcx?= NE\wdXBCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJJhmdm:pcnHmeIVlKGmwIFLhcIIw[yCwdXTlJI1wfXOnIHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC2dX3vdkBoem:5dHigZZQhOC53IH3nM4toNCCrcDDh[I1qdmm|dHXy[YQhfGi{ZXWgeIlu\XNiYTD3[YVsKG[xcjD0bJJm\SC5ZXXrd{B{fGG{dHnu[{Bnem:vIHThfUA4KGGodHXyJJR2dW:{IHPlcIwhcW6xY4XsZZRqd25? NV;l[lBsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVMzOTZpPkOwNVk{OjF4PD;hQi=>
MCF7 M1r6fWFvfGm2dX3vdkBie3OjeR?= NUHicIpuOC53IH3nM4to NHPtPJg{KHenZXvz MW\BcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IIjlco9oemGodHXkJIlvKEKjbHKvZ{BvfWSnIH3veZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJGJigCCneIDy[ZN{cW:wIHH0JFAvPSCvZz;r[{whcXBiYXTtbY5qe3SncnXkJJRpemWnIITpcYV{KGFid3Xlb{Bnd3JidHjy[YUhf2Wna4Ogd5RienSrbneg[pJwdSCmYYmgO{Bi\nSncjD0eY1weiClZXzsJIlvd2O3bHH0bY9vKGK7IHntcZVvd2irc4TvZ4hmdWmlYXygZY4> M2PTV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUmzNlE3Lz5|MEG5N|IyPjxxYU6=
C3H10T1/2 NW\sO3NHTnWwY4Tpc44h[XO|YYm= NX:x[JZTOC53IIXN MWKyOEBpenN? Mn3hRYN1cX[jdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDuc44ufGG{Z3X0bY5oKHOrUl7BJJRz\WG2ZXSgcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IHXm[oVkfCCxbjDWSHIh\XiycnXzd4lwdiCjdDCwMlUhfU1iYX\0[ZIhOjRiaILzJIJ6KHGSQ2KgZY5idHm|aYO= M1zHdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{OwPVg1Lz5{NEezNFk5PDxxYU6=
C3H10T1/2 M360TWZ2dmO2aX;uJIF{e2G7 MoDPNE42KHWP NUfjOo9WOjRiaILz NW\4U4FYSWO2aY\heIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBXTFJva37vZ4tld3ewIH3veZNmKEN|SEGwWFEwOiClZXzsd{Bie3Onc4Pl[EBieyCnZn\lZ5Qhd25iVlTSJIV5eHKnc4Ppc44h[XRiMD61JJVOKGGodHXyJFI1KGi{czDifUByWEOUIHHuZYx6e2m| NWjqUm1RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3N|A6QDRpPkK0O|MxQTh2PD;hQi=>
C3H10T1/2 MVzGeY5kfGmxbjDhd5NigQ>? MWiwMlUhfU1? NYPHdIluOjRiaILz NUW4[29zTW[oZXP0JI9vKEO\UEK0RVEhWk6DIHzleoVtKGmwIH7vck11[XKpZYTpcoche2mUTlGgeJJm[XSnZDDtc5V{\SCFM1ixNHQyNzJiY3XscJMh[XRiMD61JJVOKGGodHXyJFI1KGi{czDifUByWEOUIHHuZYx6e2m| MonmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5M{C5PFQoRjJ2N{OwPVg1RC:jPh?=
C3H10T1/2 NFPaUmpHfW6ldHnvckBie3OjeR?= NUL5eoZTOC53IIXN MlO4NlQhcHK| MXTJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6KGmwIH7vck11[XKpZYTpcoche2mUTlGgeJJm[XSnZDDtc5V{\SCFM1ixNHQyNzJiY3XscJMh[XO|ZYPz[YQh[XNiZX\m[YN1KG:wIG\EVkBmgHC{ZYPzbY9vKGG2IECuOUB2VSCjZoTldkAzPCCqcoOgZpkheVCFUjDhcoFtgXOrcx?= NITC[oQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEezNFk5PCd-MkS3N|A6QDR:L3G+
C3H10T1/2 M1jUSWZ2dmO2aX;uJIF{e2G7 MVywMlUhfU1? Mmn6NlQhcHK| M2LiRWlvcGmkaYTpc44hd2ZiaHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gWmRTNWuwb3Pr[I94diCvb4Xz[UBEO0hzMGSxM|Ih[2WubIOgZZN{\XO|ZXSgZZMh\W[oZXP0JI9vKF[GUjDlfJBz\XO|aX;uJIF1KDBwNTD1UUBi\nSncjCyOEBpenNiYomgdXBEWiCjbnHsfZNqew>? MkDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5M{C5PFQoRjJ2N{OwPVg1RC:jPh?=
LX2 MVjGeY5kfGmxbjDhd5NigQ>? NVvxU3ltOTByIH7N MlHXNlQhcHK| Mmm5WJJidnOjY4TpeoF1cW:wIH;mJHZFWiCrbjDoeY1idiCOWEKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCWR1\i[ZRiOS2rbnT1Z4VlKEORTEHBNUBuWk6DIHX4dJJme3Orb36gZZQhOTByIH7NJIFnfGW{IEK0JIhzeyCkeTDRMXBEWiCjbnHsfZNqew>? NGXNUJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO1NFk6QSd-M{CzOVA6QTl:L3G+
ROS 17/2.8 NHLpeVhHfW6ldHnvckBie3OjeR?= MlrHNVYhcHK| M{Wwd2lv\HWldHnvckBw\iCYRGKgeJJidnOlcnnweIlwdmGuIHHjeIl3cXS7IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiMkStbJllem:6eXzhd4Uh\2WwZTDlfJBz\XO|aX;uJIlvKHKjdDDSU3MhOTdxMj64JINmdGy|IHHmeIVzKDF4IHjyd{BjgSCudXPp[oVz[XOnIILldJJ1\XJiYYPzZZk> NX6wNXEzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|Q6OjFpPkK2OVc1QTJzPD;hQi=>
HL60 NY\DeYRxTnWwY4Tpc44h[XO|YYm= M1fmSlMxKHSxIEGwNFAhdk1? MXe0PEBpenN? NVm4[G9TUW6mdXP0bY9vKG:oIHPlcIwh\GmoZnXy[Y51cWG2aX;uJIlvKGi3bXHuJGhNPjBiY3XscJMh[XO|ZYPz[YQh[XNibXH0eZJqdmdibX;ydIhwdG:paXPhcEBkcGGwZ3XzJIF1KDNyIITvJFExODBibl2gZYZ1\XJiNEigbJJ{KGK7IGfybYdpfCe|LVfp[Y1{[SC|dHHpcolv\y2kYYPl[EBnd3K2eTDtbYNzd3Olb4DpZ{B3cWW5IHHzd4F6 MoLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkexOFkoRjJ3MUK3NVQ6RC:jPh?=
SW480-ADH NILYbpFHfW6ldHnvckBie3OjeR?= NHvoWpIyODBibl2= NG\q[Vc1QCCqcoO= M1HrdWlv\HWldHnvckBw\iCqdX3hckBUXzR6MD3BSGgh[2WubDDkbYZn\XKnboTpZZRqd25iYYPz[ZN{\WRiYYOg[o9zdWG2aX;uJI9nKGOxbYDhZ5Qh\XCrdHjlcIlwcWRiaYPsZY5leyCxZjDobYdpdHliYXTo[ZJmdnRiY3XscJMh[XRiMUCgK{04KE1iYX\0[ZIhPDhiaILzJIJ6KHCqYYPlMYNwdnS{YYP0JI1q[3Kxc3PvdJk> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl6OUO3PUc,OjJ7OEmzO|k9N2F-
SW480-ADH M1jGeGZ2dmO2aX;uJIF{e2G7 MXexNEBvVQ>? MWK0PEBpenN? M2rwd2lv\HWldHnvckBw\iCqdX3hckBUXzR6MD3BSGgh[2WubDDkbYZn\XKnboTpZZRqd25iYYPz[ZN{\WRiYYOg[o9zdWG2aX;uJI9nKGOxbYDhZ5Qh\XCrdHjlcIlwcWRiaYPsZY5leyCxZjDobYdpdHliYXTo[ZJmdnRiY3XscJMh[XRiMUCgK{05KE1iYX\0[ZIhPDhiaILzJIJ6KHCqYYPlMYNwdnS{YYP0JI1q[3Kxc3PvdJk> M2G1ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUi5N|c6Lz5{Mkm4PVM4QTxxYU6=
MCF7 M3e5TmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NV30cmNVOTByIH7N MlHvOFghcHK| MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYYSgNVAhLy15IF2gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQu[2:wdILvcC=> NX3NepFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5PFk{PzlpPkKyPVg6Ozd7PD;hQi=>
CLL MXjGeY5kfGmxbjDhd5NigQ>? M2XzWVExOCCwTR?= NUf3[WM1OjRiaILz M{T6dmVn\mWldDDvckBEYVB{NFGxJIlvKGi3bXHuJJBzcW2jcomgR2xNKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIG\EVk1z\We3bHH0[YQhT0GGRES1ZYxxcGFibWLORUBmgHC{ZYPzbY9vKGG2IEGwNEBvVSCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUByfWGwdHn0ZZRqfmViUmStVGNTKG2ndHjv[C=> Ml\HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNEizPVIoRjJ3MUS4N|kzRC:jPh?=
CLL NYrMOnNzTnWwY4Tpc44h[XO|YYm= MVexNFAhdk1? NX3ZbmxZOjRiaILz MUHF[oZm[3Rib36gR3lROjSDMTDpckBpfW2jbjDwdolu[XK7IFPMUEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCYRGKtdoVofWyjdHXkJGNFU05zQTDtVm5CKGW6cILld5Nqd25iYYSgNVAxKG6PIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KHG3YX70bZRifGm4ZTDSWE1RS1JibXX0bI9l NUjVWnc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOFg{QTJpPkK1NVQ5Ozl{PD;hQi=>
Assay
Methods Test Index PMID
Western blot CyclinD1 ; IRE1α / p-eIF2α / eIF2α / Atf4 / CHOP ; p-PERK / PERK ; FGFR1 / FGFR2 / FGFR3 / FGFR4 ; Wnt10b / Wnt16 28643892 29776409 30149605

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 416.64
Formula

C27H44O3

CAS No. 32222-06-3
Storage 3 years -20°C powder
Smiles CC(CCCC(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CC(C3=C)O)O)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04551170 Recruiting Drug: Theophylline|Drug: Placebo Pseudohypoparathyroidism|Albright Hereditary Osteodystrophy|Pseudohypoparathyroidism Type 1a Vanderbilt University Medical Center July 13 2020 Phase 2
NCT03029429 Recruiting Drug: Theophylline|Drug: Placebos Pseudohypoparathyroidism|Albright Hereditary Osteodystrophy Vanderbilt University Medical Center|Harvard University September 1 2018 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Calcitriol (RO215535) | Calcitriol (RO215535) ic50 | Calcitriol (RO215535) price | Calcitriol (RO215535) cost | Calcitriol (RO215535) solubility dmso | Calcitriol (RO215535) purchase | Calcitriol (RO215535) manufacturer | Calcitriol (RO215535) research buy | Calcitriol (RO215535) order | Calcitriol (RO215535) mouse | Calcitriol (RO215535) chemical structure | Calcitriol (RO215535) mw | Calcitriol (RO215535) molecular weight | Calcitriol (RO215535) datasheet | Calcitriol (RO215535) supplier | Calcitriol (RO215535) in vitro | Calcitriol (RO215535) cell line | Calcitriol (RO215535) concentration | Calcitriol (RO215535) nmr