Calcitriol (RO215535)

For research use only.

Catalog No.S1466 Synonyms: Topitriol, 1,25-Dihydroxyvitamin D3

32 publications

Calcitriol (RO215535) Chemical Structure

CAS No. 32222-06-3

Calcitriol (RO215535, Topitriol, 1,25-Dihydroxyvitamin D3) is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol. Solutions are best fresh-prepared.

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10mM (1mL in DMSO) EUR 491 In stock
EUR 117 In stock
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Selleck's Calcitriol (RO215535) has been cited by 32 publications

Purity & Quality Control

Choose Selective Vitamin Inhibitors

Biological Activity

Description Calcitriol (RO215535, Topitriol, 1,25-Dihydroxyvitamin D3) is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol. Solutions are best fresh-prepared.
Targets
vitamin D receptor [1]
(Cell-free assay)
In vitro

Calcitriol is a potent inhibitor of PHA-induced lymphocyte proliferation, achieving 70% inhibition of tritiated thymidine incorporation after 72 h in culture. Calcitriol suppresses interleukin-2 (IL-2) production by PHA-stimulated peripheral blood mononuclear cells in a concentration-dependent fashion. [1] Calcitriol increases the concentration of intracellular calcium ([Ca2+]i) within 5 s by mobilizing calcium from the endoplasmic reticulum and the formation of inositol 1,4, 5-trisphosphate and diacylglycerol. [2] Calcitriol can inhibit the proliferation and promote the differentiation of human prostate adenocarcinoma cells. Calcitriol causes a selective decrease in the secreted levels of type IV collagenases (MMP-2 and MMP-9). [3] Calcitriol has antiproliferative activity in squamous cell carcinoma and prostatic adenocarcinoma and enhances the antitumor activity of platinum-based agents. Calcitriol prior to paclitaxel significantly reduces clonogenic survival compared with either agent alone in the murine squamous cell carcinoma and PC-3 cells. [4] Calcitriol is a potent anti-proliferative agent in a wide variety of malignant cell types. Calcitriol effects are associated with an increase in G0/G1 arrest, induction of apoptosis and differentiation, modulation of expression of growth factor receptors. Calcitriol potentiates the antitumor effects of many cytotoxic agents and inhibits motility and invasiveness of tumor cells and formation of new blood vessels. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HCT116 MlG5R5l1d3SxeHnjxsBie3OjeR?= M4LSN|EzOCCq M4\PdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiClZXzsJINwfW62IHHmeIVzKDF{MDDodpMtKEmFNUC9NYUuODVizszN MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{NkC2PEc,OjJ7Mk[wOlg9N2F-
HOS NHHHR5NHfW6ldHnvckBie3OjeR?= NX\hS4g5SWO2aY\peJkh[XRiaIXtZY4hXkSUIHX4dJJme3OnZDDpckBpfW2jbjDIU3Mh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDwS2w{NWiRYzygdGNFVkFvaG\EVkBidmRicHjSUE1VUyCjc4Pld5Nm\CCjczDhd5Nme3OnZDDhd{B1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBu\WG|dYLl[EAzPCCqcoOgdI9{fCCrbn\lZ5Rqd25iYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMECwNUDPxE1w M3OycFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEi5N|M1Lz5{MUi4PVM{PDxxYU6=
SK-MEL-188 NXzOUY85T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3fOPFQ5KGi{cx?= M1\iRWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHNMNU2HTD2xPFgh[2WubIOgcYVie3W{ZXSgZYZ1\XJiNEigbJJ{KGK7IF3UV{9RVVNiYYPzZZktKEmFNUCgQUAxNjByMECxJO69VS5? NIDiVng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C3NFc4QSd-MkewO|A4Pzl:L3G+
osteosarcoma cells MWfGeY5kfGmxbjDhd5NigQ>? M1LU[GVn\mWldDDvckBXTFJidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGmwIHj1cYFvKG:|dHXvd4Fz[2:vYTDj[YxteyxiRVO1NEA:KDBwMECwNFExPiEQvF2u NUfzbFR[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwOVQzOzBpPkG4NFU1OjNyPD;hQi=>
HOS/SF Mm\NSpVv[3Srb36gZZN{[Xl? MX[yOEBpenN? M3:0WnRz[W6|YXP0bZZifGmxbjDv[kBXTFJvbXXkbYF1\WRib4P0[Y9k[WylaX6gdJJwdW:2ZYKgbY4hcHWvYX6gTG9UN1OIIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwNFAzPiEQvF2u MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEeyPEc,OjR7MEC3Nlg9N2F-
LM05e MoDCRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MUO3NkBpenN? MV7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKEyPMEXlJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygcpVu[mW{IHHmeIVzKDd{IHjyd{BjgSCZU2SgZZN{[XluIFnDOVAhRSByLkCwNFA{KM7:TT6= NFG5fW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFM{QSd-MkS5NFA{Ozl:L3G+
HEK293 MU\GeY5kfGmxbjDhd5NigQ>? MX\UdoFve3KncILld5Nqd25ib3[gWnAyPi22YXfn[YQhXkSUIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCqYYLic5JqdmdicFPNXE1ISUx2LV7Dc3Ih[W6mIF3INVAxMFWDUzmgXEA1fGtvTGXDJJJmeG:{dHXyJJBt[XOvaXSgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iTlPvVkBz\WO{dXn0cYVvfCCkeTDi[ZRiNWejbHHjeI9{cWSjc3WgdoVxd3K2ZYKg[4Vv\SCkYYPl[EBu[W2vYXzpZY4hfHexLXj5ZpJq\CCjc4PhfUwhUUN3MDC9JFAvODByMEWg{txONg>? NED3WZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUm4PVgyPyd-Mkm5PFk5OTd:L3G+
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HEK293T NGqzSIxHfW6ldHnvckBie3OjeR?= M3\IPGFod26rc4SgZYN1cX[rdImgZZQhT0GONDDEUmEu[mmwZHnu[{Bld22jaX6g[pV{\WRiVlTSJEh2dmuwb4fuJI9zcWerbjmgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDVRXMu[myjIFjFT{AzQTOWIHPlcIx{KGG|c3Xzd4VlKGG|IHLleIEudGGldHHtZZNmKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGK7IF\SSXQu[mG|ZXSgS4Vv\UKOQYrldkBie3OjeTygTWM2OCB;IECuNFAxODl3MzFOwG0v NIm4N449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEG0OFY6Pyd-M{CxOFQ3QTd:L3G+
HEK293 MmT4SpVv[3Srb36gZZN{[Xl? NV\J[FVCSWexbnnzeEBi[3Srdnn0fUBifCCYRGKgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHPveJJidnOoZXP0[YQhf2m2aDDOR49TKGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJG5Ed1JicnXjdpVqfG2nboSgZpkhfHexLXj5ZpJq\CCjc4PhfUwhUUN3MDC9JFAvODByMTFOwG0v MnXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ4MUO0NlAoRjJ4NkGzOFIxRC:jPh?=
HEK293 NVzTOVh6TnWwY4Tpc44h[XO|YYm= MkDsNVYhfG9iMkSgbJJ{ M2K1TGFod26rc4SgZYN1cX[rdImgZZQhXkSUIHnuJIh2dWGwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[W[2ZYKgNVYhfG9iMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODByMUKg{txONg>? NH[xdVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[xN|QzOCd-Mk[2NVM1OjB:L3G+
ROS 17/2.8 M{DjfWZ2dmO2aX;uJIF{e2G7 MVOxOkBpenN? NUHxN4tTSWO2aY\peJkh[XRiVlTSJIlvKHKjdDDSU3MhOTdxMj64JINmdGy|IHHzd4V{e2WmIHHzJJRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJI9nKDJ2LXj5[JJwgHmuYYPlJIdmdmVicILvcY91\XJiYX\0[ZIhOTZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFAxOiEQvF2u NWK0OVF{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxOlc5QTNpPkG5NVY4QDl|PD;hQi=>
osteosarcoma cells MnLvSpVv[3Srb36gZZN{[Xl? NV\ifINuSWO2aY\peJkh[XRiVlTSJIlvKHKjdDDvd5Rmd3OjcnPvcYEh[2WubIOgZZN{\XO|ZXSgZZMhOjRvaInkdo95gWyjc3WgeJJidnOlcnnweIlwdiCkeTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xODB{IN88UU4> MorlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7NkC5NlQoRjF5OU[wPVI1RC:jPh?=
ROS 17/2.8 NInG[FdHfW6ldHnvckBie3OjeR?= MXSxOkBpenN? M{HwdWFkfGm4aYT5JIF1KHKjdDDy[YNwdWKrbnHueEBnfWyuIHzlcod1cCCYRGKg[ZhxemW|c3XkJIlvKHKjdDDSU3MhOTdxMj64JINmdGy|IITyZY5{\mWldHXkJJdqfGhiMkStbJllem:6eXzhd4Uh\2WwZTDwdo9ud3SncjDhd5Nme3OnZDDhd{B1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBi\nSncjCxOkBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMECyJO69VS5? NV7WenZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi3NlIyOzBpPkG4O|IzOTNyPD;hQi=>
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ROS 17/2.8 MXnGeY5kfGmxbjDhd5NigQ>? MlX1NVYhcHK| NVzyT5hsSWexbnnzeEBi[3Srdnn0fUBifCC{YYSgWmRTKGmwIGLPV{AyPy9{LkigZ4VtdHNidILhcpNn\WO2ZXSge4l1cCCFeYCyOIEyKHKncH;yeIVzKHCuYYPtbYQh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gR5lxOjSjMTD0doFve2O{aYD0bY9vKGGodHXyJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwNFIh|ryPLh?= NH7jb4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCxPFkyQCd-MkKwNVg6OTh:L3G+
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osteosarcoma cells NULQd5V3TnWwY4Tpc44h[XO|YYm= MXnUdoFve2GldHn2ZZRqd25ib3[gWmRTKGmwIILheEBwe3Snb4PhdoNwdWFiY3XscJMh[XO|ZYPz[YQh[XNiMkStbJllem:6eXzhd4UhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWQ2OCB;IECuNFAxOiEQvF2u NIHkPXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[zNFQ1PCd-Mk[2N|A1PDR:L3G+
osteosarcoma cells MV3GeY5kfGmxbjDhd5NigQ>? NEHkemwyPiCqcoO= NWPQenB2UW6qaXLpeIlwdiCxZjDWSHIucW6mdXPl[EAzPC2qeXTyc5h6dGG|ZTD0doFve2O{aYD0bY9vKGmwIILheEBwe3Snb4PhdoNwdWFiY3XscJMh[W[2ZYKgNVYhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVS1NEA:KDBwMECwNkDPxE1w NUHCUoNURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PVAxOTBpPkKyOFkxODFyPD;hQi=>
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C3H10T1/2 NIWzdWFHfW6ldHnvckBie3OjeR?= Mon2NE42KHWP MUeyOEBpenN? NHPmfYtKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI5wdi22YYLn[ZRqdmdic3nSUmEhfHKnYYTl[EBud3W|ZTDDN2gyOFRzL{KgZ4VtdHNiYYPz[ZN{\WRiYYOg[YZn\WO2IH;uJHZFWiCneIDy[ZN{cW:wIHH0JFAvPSC3TTDh[pRmeiB{NDDodpMh[nlicWDDVkBidmGueYPpdy=> NV7wbFlTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3N|A6QDRpPkK0O|MxQTh2PD;hQi=>
C3H10T1/2 M1;MVGZ2dmO2aX;uJIF{e2G7 M1zLOVAvPSC3TR?= M4q4elI1KGi{cx?= Ml\HTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDWSHIuc26xY3vkc5dvKG2xdYPlJGM{UDFyVEGvNkBk\WyuczDhd5Nme3OnZDDhd{Bm\m[nY4Sgc44hXkSUIHX4dJJme3Orb36gZZQhOC53IIXNJIFnfGW{IEK0JIhzeyCkeTDxVGNTKGGwYXz5d4l{ M4H1RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{OwPVg1Lz5{NEezNFk5PDxxYU6=
LX2 M1q5UGZ2dmO2aX;uJIF{e2G7 NF\5VJYyODBibl2= NVzQb5hwOjRiaILz NXT3WGdwXHKjboPhZ5RqfmG2aX;uJI9nKF[GUjDpckBpfW2jbjDMXFIh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDUS2Zj\XSjMT3pcoR2[2WmIFPPUFFCOSCvUl7BJIV5eHKnc4Ppc44h[XRiMUCwJI5OKGGodHXyJFI1KGi{czDifUBSNVCFUjDhcoFtgXOrcx?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN3MEm5PUc,OzB|NUC5PVk9N2F-
ROS 17/2.8 NF3iW5dHfW6ldHnvckBie3OjeR?= NHfxV2UyPiCqcoO= NVfjN5RlUW6mdXP0bY9vKG:oIG\EVkB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgZZN{\XO|ZXSgZZMh[WO2aY\heIlwdiCxZjCyOE1pgWS{b4j5cIF{\SCpZX7lJIV5eHKnc4Ppc44hcW5icnH0JHJQWyBzNz:yMlgh[2WubIOgZYZ1\XJiMU[gbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxenSncjDhd5NigQ>? NFyyO4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OFkzOSd-Mk[1O|Q6OjF:L3G+
HL60 NF6zSG1HfW6ldHnvckBie3OjeR?= MoDuN|AhfG9iMUCwNEBvVQ>? MYC0PEBpenN? MWnJcoR2[3Srb36gc4Yh[2WubDDkbYZn\XKnboTpZZRqd25iaX6gbJVu[W5iSFy2NEBk\WyuczDhd5Nme3OnZDDhd{Bu[XS3cnnu[{Bud3KyaH;sc4dq[2GuIHPoZY5o\XNiYYSgN|AhfG9iMUCwNEBvVSCjZoTldkA1QCCqcoOgZpkhX3KrZ3j0K5MuT2mnbYPhJJN1[WmwaX7nMYJie2WmIH\vdpR6KG2rY4Lvd4NweGmlII\p[Zch[XO|YYm= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF{N{G0PUc,OjVzMkexOFk9N2F-
SW480-ADH MV7GeY5kfGmxbjDhd5NigQ>? NUTTdWtSOTByIH7N NIH2cIQ1QCCqcoO= NInYc4JKdmS3Y4Tpc44hd2ZiaIXtZY4hW1d2OECtRWRJKGOnbHyg[Iln\mW{ZX70bYF1cW:wIHHzd4V{e2WmIHHzJIZwem2jdHnvckBw\iClb33wZYN1KGWyaYTo[Yxqd2mmIHnzcIFv\HNib3[gbIlocGy7IHHkbIVz\W62IHPlcIx{KGG2IEGwJEcuPyCPIHHmeIVzKDR6IHjyd{BjgSCyaHHz[U1kd262cnHzeEBucWO{b4Pjc5B6 MmLFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OEmzO|koRjJ{OUi5N|c6RC:jPh?=
SW480-ADH NUXHfWNNTnWwY4Tpc44h[XO|YYm= NYnYZ|N5OTBibl2= MkfFOFghcHK| NEeweohKdmS3Y4Tpc44hd2ZiaIXtZY4hW1d2OECtRWRJKGOnbHyg[Iln\mW{ZX70bYF1cW:wIHHzd4V{e2WmIHHzJIZwem2jdHnvckBw\iClb33wZYN1KGWyaYTo[Yxqd2mmIHnzcIFv\HNib3[gbIlocGy7IHHkbIVz\W62IHPlcIx{KGG2IEGwJEcuQCCPIHHmeIVzKDR6IHjyd{BjgSCyaHHz[U1kd262cnHzeEBucWO{b4Pjc5B6 NITJXI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm4PVM4QSd-MkK5PFk{Pzl:L3G+
MCF7 MonTRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NFzIfmgyODBibl2= NIrsc5o1QCCqcoO= MUnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYYSgNVAhLy15IF2gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQu[2:wdILvcC=> NHT6R209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm4PVM4QSd-MkK5PFk{Pzl:L3G+
CLL MWDGeY5kfGmxbjDhd5NigQ>? Ml:2NVAxKG6P MUeyOEBpenN? M3vE[WVn\mWldDDvckBEYVB{NFGxJIlvKGi3bXHuJJBzcW2jcomgR2xNKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIG\EVk1z\We3bHH0[YQhT0GGRES1ZYxxcGFibWLORUBmgHC{ZYPzbY9vKGG2IEGwNEBvVSCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUByfWGwdHn0ZZRqfmViUmStVGNTKG2ndHjv[C=> MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF2OEO5Nkc,OjVzNEizPVI9N2F-
CLL NF7Q[ZNHfW6ldHnvckBie3OjeR?= MnLPNVAxKG6P MlXwNlQhcHK| M2XCTWVn\mWldDDvckBEYVB{NFGxJIlvKGi3bXHuJJBzcW2jcomgR2xNKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIG\EVk1z\We3bHH0[YQhS0SNTkHBJI1TVkFiZYjwdoV{e2mxbjDheEAyODBibl2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nlicYXhcpRqfGG2aY\lJHJVNVCFUjDt[ZRpd2R? M3XXVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUS4N|kzLz5{NUG0PFM6OjxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
CyclinD1 ; 

PubMed: 28643892     


Immunoblot analysis of cyclinD1 in AXT cells treated with the indicated concentrations of calcitriol for 14 h. 

IRE1α / p-eIF2α / eIF2α / Atf4 / CHOP ; 

PubMed: 28643892     


Immunoblot analysis of Ire1a and total or phosphorylated (p-) forms of eIf2a, Atf4 and Chop in AXT cells treated with 5 lM calcitriol for the indicated times.

p-PERK / PERK ; 

PubMed: 28643892     


Immunoblot analysis of total or phosphorylated (p-) forms of Perk and Atf4 in AXT cells treated with 5 lM calcitriol for the indicated times or with 1 lM thapsigardin (Thap) for 1.5 h.

FGFR1 / FGFR2 / FGFR3 / FGFR4 ; 

PubMed: 29776409     


Representative immunoblots and average data (n = 6) of FGFR1, FGFR2, FGFR3, and FGFR4 from control and calcitriol (1 and 10 nM)-treated HL-1 cells.

Wnt10b / Wnt16 ; 

PubMed: 30149605     


Wnt10b and Wnt 16 protein expression in calcitriol-treated osteoclasts. With calcitriol treatment of 10 to 100 nM overnight, Western blot analysis showed a significant increase in Wnt 10b expression. Wnt 16 expression in osteoclasts after different doses of calcitriol was not significantly different at the protein level. Actin protein served as a loading control. 

28643892 29776409 30149605

Protocol

Solubility (25°C)

In vitro DMSO 83 mg/mL (199.21 mM)
Water Insoluble
Ethanol '''83 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 416.64
Formula

C27H44O3

CAS No. 32222-06-3
Storage 3 years -20°C powder
Synonyms Topitriol, 1,25-Dihydroxyvitamin D3
Smiles CC(CCCC(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CC(C3=C)O)O)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Step 2: Enter the in vivo formulation ()
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01588522 Completed Drug: compound 31543 Calcitriol Breast Cancer|Cervical Cancer|Endometrial Cancer|Ovarian Cancer|Primary Peritoneal Carcinoma|Bone Sarcoma|Cancer|Solid Tumor Berg LLC July 2012 Phase 1
NCT01039181 Unknown status Drug: Calcitriol Advanced Intrahepatic Cholangiocarcinoma National Science and Technology Development Agency Thailand|Khon Kaen University January 2010 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID