For research use only.

Catalog No.S7774

22 publications

SU6656 Chemical Structure

CAS No. 330161-87-0

SU 6656 is a selective Src family kinase inhibitor with IC50 of 280 nM, 20 nM, 130 nM, and 170 nM for Src, Yes, Lyn, and Fyn, respectively.

Selleck's SU6656 has been cited by 22 publications

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Biological Activity

Description SU 6656 is a selective Src family kinase inhibitor with IC50 of 280 nM, 20 nM, 130 nM, and 170 nM for Src, Yes, Lyn, and Fyn, respectively.
YES [1] Lyn [1] Fyn [1] Src [1]
20 nM 130 nM 170 nM 280 nM
In vitro

In NIH 3T3 cells, SU 6656 inhibits the PDGF-stimulated S-phase induction with IC50 of 0.3-0.4 μM. SU 6656 also inhibits PDGF- and serum-mediated NIH 3T3 cell proliferation, as well as epidermal growth factor and colony-stimulating factor 1-stimulated DNA synthesis in normal and colony-stimulating factor 1 receptor transfected NIH 3T3 cells. SU6656 inhibits PDGF-stimulated c-Myc induction and ERK2 activation.[1] Pretreating Jurkat T-cells with SU 6656 leads to increased VSV-G luciferase activity.[2] SU 6656 impairs TGF-β-mediated upregulation of CTGF mRNA and protein in proximal epithelial HKC-8 cells, and also reduces CTGF expression in cells exposed to autocrine growth factors. SU 6656 interferes with Aurora kinase activity resulting in inhibition of cell division and formation multilobular nuclei.[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Sf9 insect cells MUjGeY5kfGmxbjDhd5NigQ>? Ml7nTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKaYOteIFo\2WmIGLFWEBmgHC{ZYPz[YQhcW5iU3[5JIlve2WldDDj[YxteyxiSVO1NF0xNjd5IN88US=> NXfDeZE5OjBzMUewNFQ>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
phospho-c-Abl / c-Abl / phospho-c-Src / c-Src ; 

PubMed: 23049975     

Protein levels of phosphorylated c-Abl (Tyr245), c-Abl, phosphorylated c-Src (Tyr416), c-Src, and GAPDH in NSC-34 cells overexpressing human wild-type or mutant SOD1s treated with various concentrations of SU6656 were measured by western blot. Cells were cultured in serum-free culture medium with doxycycline (Dox, 2 µg/ml), and western blot was performed at 24 h after SU6656 addition.

p-STAT3 / STAT3 ; 

PubMed: 19284568     

SU6656 inhibits phosphorylation of Src and STAT3 in OSA lines. Canine and human OSA cell lines were serum starved then left untreated or incubated with SU6656 for 2 hours. Cells were collected and protein separated by SDS-PAGE, followed by Western blotting for pSrc (Y416), pSTAT3 (Y705), total Src, and total STAT3.

p-p38 / p38 / p-CREB / CREB ; 

PubMed: 30816523     

Increased phosphorylation of p38 or CREB by the Src inhibitor SU6656 (1 µM) was determined by western blotting.

23049975 19284568 30816523

PubMed: 30816523     

Increased expression of p-p38 or p-CREB by SU6656 (1 µM) or PP2 (1 µM) was determined by immunofluorescence. Scale bars, 20 µm.

E-cadherin / Fibronectin ; 

PubMed: 23246962     

Fibronectin (orange) and E-cadherin (green) staining of H358 cells under normoxia or hypoxia for 56 h ± Src inhibitor. Scale bar, 100 µm.

30816523 23246962
In vivo SU 6656 markedly and dose-dependently attenuates mecamylamine-induced experimental nicotine withdrawal syndrome in mice measured in terms of WSS and anxiety score.[4] Once-daily administration of SU 6656 (1.5, 3, and 6 mg/kg, i.p.) markedly and dose-dependently attenuates the naloxone-induced morphine withdrawal syndrome.[5]


Kinase Assay:[1]
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Biochemical kinase assays for IC50 determination and kinetic studies:

IC50 measurements are made using poly-Glu–Tyr (4:1), or, in the case of Lck, poly-Lys–Tyr (4:1) as a peptide substrate. The divalent cation is 20 mM MgCl2 (in the case of Src, Fyn, Yes, Lyn, Csk, Frk, or Abl) or 10 mM MnCl2 (in the case of FGFR1, IGF1R, Lck, or Met). The final ATP concentrations are as follows: Src, 10 μM; Fyn, 6 μM; Yes, 100 μM; Lyn, 2 μM; Csk, 10 μM; Frk, 10 μM; Abl, 4 μM; FGFR1, 10 μM; IGF1R, 2 μM; Lck, 2 μM; Met, 5 μM; PDGFR, 6 μM. IC50 measurements of PDGFRb autophosphorylation are determined on immunoprecipitated PDGFRb. Km values are calculated using the Eadie-Hofstee method.
Cell Research:[3]
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  • Cell lines: HKC-8 cells
  • Concentrations: 5 μM
  • Incubation Time: 48 h
  • Method: HKC-8 cells are seeded at different cells densities (10,000 cells/cm2 and 50,000 cells/cm2) and cultured in the presence or absence of SU 6656 for 24 and 48 h. Bright field pictures of cells are recorded by Olympus CK40 microscope using Leica DC Viewer software.
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: Inbred Swiss albino male mice
  • Dosages: 3 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 74 mg/mL warmed (199.21 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50%dd H2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.45


CAS No. 330161-87-0
Storage powder
in solvent
Synonyms N/A
Smiles CN(C)S(=O)(=O)C1=CC2=C(C=C1)NC(=O)C2=CC3=CC4=C(N3)CCCC4

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Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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% DMSO % % Tween 80 % ddH2O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID