Name: STF-31
Brand: Selleck Chemicals
SKU: S7931
Availability: InStock
Rating: 5 (13 reviews)

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Quality Control

Batch: Purity: 99.83%

99.83

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other GLUT Products	BAY-876 WZB117 DRB18 Glut1 Antibody [C8B12] GLUT4 Antibody [H5N16] KL-11743 Glut4 Antibody [G3B7] AS160 Antibody [L14F10] MUC16 Antibody [B21H3] Glucose Transporter GLUT2 Antibody [E14F15]

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Incubation Time	Activity Description
A2780 cells	Function assay	48 h	Inhibition of NAMPT in human A2780 cells assessed as reduction in NAD level after 48 hrs by LC-MS/MS analysis, IC50=0.024 μM
RCC4 cells	Cytotoxicity assay	4 days	Cytotoxicity against human RCC4 cells after 4 days by XTT assay, IC50=0.16 μM
HT1080 cells	Proliferation assay	96 h	Antiproliferative activity against human HT1080 cells after 96 hrs by CyQuant-based assay, IC50=0.213 μM
PC3 cells	Proliferation assay	96 h	Antiproliferative activity against human PC3 cells after 96 hrs by CyQuant-based assay, IC50=0.217 μM
HCT116 cells	Proliferation assay	96 h	Antiproliferative activity against human HCT116 cells after 96 hrs by CyQuant-based assay, IC50=0.271 μM
MIAPaCa2 cells	Proliferation assay	96 h	Antiproliferative activity against human MIAPaCa2 cells after 96 hrs by CyQuant-based assay, IC50=0.855 μM
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 85 mg/mL (200.69 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 25 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	423.53	Formula	C₂₃H₂₅N₃O₃S	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	724741-75-7	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(C)(C)C1=CC=C(C=C1)S(=O)(=O)NCC2=CC=C(C=C2)C(=O)NC3=CN=CC=C3

Mechanism of Action

Targets/IC₅₀/Ki	GLUT1
In vitro	STF-31 selectively kills RCCs by specifically targeting glucose uptake through GLUT1. This compound significantly inhibits lactate production and extracellular acidification in VHL-deficient cells by about 60%, and decreases glycolysis by decreasing glucose transport. In NAPRT1-expressing cells, it shows cytotoxicity by inhibiting the enzymatic activity of NAMPT. It is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures.
In vivo	In mice with VHL-deficient RCC xenografts, a more soluble analog of STF-31 (11.6 mg/kg, i.p.) markedly delays tumor growth.
References	[1] https://pubmed.ncbi.nlm.nih.gov/21813754/ [2] https://pubmed.ncbi.nlm.nih.gov/25058389/ [3] https://pubmed.ncbi.nlm.nih.gov/25068131/

Applications

Methods	Biomarkers	Images	PMID
Western blot	TFCP2 / ASIC2a p-EGFR / HIF-1α / c-Myc / Glut-1		28725010
Immunofluorescence	ASIC2a / TFCP2		28725010

Tech Support

Handling Instructions

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STF-31 GLUT inhibitor

Chemical Structure

Molecular Weight: 423.53