WZB117

Catalog No.S7927

For research use only.

WZB117 is an inhibitor of Glucose Transporter 1 (GLUT1). It inhibited cell proliferation in lung cancer A549 cells and breast cancer MCF7 cells with an IC50 of approximately 10 μM.

WZB117 Chemical Structure

CAS No. 1223397-11-2

Selleck's WZB117 has been cited by 8 Publications

Purity & Quality Control

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Biological Activity

Description WZB117 is an inhibitor of Glucose Transporter 1 (GLUT1). It inhibited cell proliferation in lung cancer A549 cells and breast cancer MCF7 cells with an IC50 of approximately 10 μM.
Targets
GLUT1 [1]
(In cancer cells)
10μM
In vitro

WZB117 inhibits glucose transport in human red blood cells. Cancer cell treatment with WZB117 leads to decreases in levels of Glut1 protein, intracellular ATP, and glycolytic enzymes. All these changes are followed by increase in ATP-sensing enzyme AMP-activated protein kinase (AMPK) and declines in cyclin E2 as well as phosphorylated retinoblastoma, resulting in cell-cycle arrest, senescence, and necrosis[1].

In vivo Daily intraperitoneal injection of WZB117 at 10mg/kg resultes in 70% reduction in tumor volume of human A549 lung cancer grafted on nude mice[1].

Protocol (from reference)

Cell Research:

Cell viability assay[1]

  • Cell lines: cancer cell 
  • Concentrations: 10μM
  • Incubation Time: 24 or 48 hours
  • Method:

    Cells are treated with compound WZB117 for 24 or 48 hours. Mock-treated and glucose deprivation samples served as negative and positive controls, respectively.

Animal Research:

[1]

  • Animal Models: Nude mice
  • Dosages: 10mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 368.31
Formula

C20H13FO6

CAS No. 1223397-11-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC(=C1)O)C(=O)OC2=C(C(=CC=C2)F)OC(=O)C3=CC(=CC=C3)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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