For research use only.
CAS No. 1263-89-4
Paromomycin Sulfate is an aminoglycoside antibiotics inhibiting protein synthesis in non-resistant cells by binding to 16S ribosomal RNA.
Purity & Quality Control
Choose Selective Selection Antibiotics for Transfected Cell Inhibitors
|Description||Paromomycin Sulfate is an aminoglycoside antibiotics inhibiting protein synthesis in non-resistant cells by binding to 16S ribosomal RNA.|
Paromomycin is an aminoglycoside that is active against Gram-negative and many Gram-positive bacteria as well as some protozoa and cestodes.  In vitro analysis on the sensitivity of amastigotes in a murine macrophage model showed that L. major and L. tropica isolates (ED50s in the range of 1 to 5 μM) are more sensitive than L. braziliensis (ED50, <12 μM) and L. mexicana (ED50, 39 μM) isolates. L. donovani shows intermediate sensitivity (ED50, 6 to 18 μM), except for one Indian strain, DD8, which had an ED50 of >150 μM. 
|In vivo||In both experimental models and clinical cases of CL, lesions caused by L. major treated with paromomycin ointment resolved faster and more completely than lesions caused by L. amazonensis and L. panamensis. |
|In vitro||Water||100 mg/mL (140.11 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03399955||Recruiting||Drug: Paromomycin|Drug: Ambisome|Drug: Miltefosine||PKDL - Post-Kala-Azar Dermal Leishmanioid||Drugs for Neglected Diseases||May 9 2018||Phase 2|
|NCT00216346||Completed||Drug: Paromomycin sulfate|Drug: Amphotericin B||Visceral Leishmaniasis||PATH|World Health Organization||June 2003||Phase 3|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.