MNS (3,4-Methylenedioxy-β-nitrostyrene)

For research use only.

Catalog No.S4921 Synonyms: MDBN

2 publications

MNS (3,4-Methylenedioxy-β-nitrostyrene) Chemical Structure

CAS No. 1485-00-3

MNS is a tyrosine kinase inhibitor, inhibits Syk, Src, p97 with IC50 of 2.5 μM, 29.3 μM and 1.7 μM, respectively.

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RMB 806.15 In stock
RMB 1631.31 In stock
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Selleck's MNS (3,4-Methylenedioxy-β-nitrostyrene) has been cited by 2 publications

1 Customer Review

  • WT mouse primary macrophages were untreated as control (lane 1) or treated with 0.1μg/ml E. coli LPS (lane 5-8), 10μM E-64 (lane 2 and 6), 250μM PMSF (lane 3 and 7), or 20μM MNS (lane 4 and 8). The cells were continuously cultured for 16 hrs. Extracts from whole cells or nuclei were separately purified and subjected to WB analysis with antibody against pERK2, LITAF, or actin/tubulin as control.

    Toxicol Res (Camb), 2016, 5(1):188-196. MNS (3,4-Methylenedioxy-β-nitrostyrene) purchased from Selleck.

Purity & Quality Control

Choose Selective p97 Inhibitors

Biological Activity

Description MNS is a tyrosine kinase inhibitor, inhibits Syk, Src, p97 with IC50 of 2.5 μM, 29.3 μM and 1.7 μM, respectively.
Features The nitro group of MNS (3,4-Methylenedioxy-β-nitrostyrene) is essential for its antiplatelet effect.
Targets
p97 [2] Syk [1] Src [1]
1.7 μM 2.5 μM 29.3 μM
In vitro

MNS (3,4-methyl-enedioxy-β-nitrostyrene) completely inhibits 2 μM U46619-(a thromboxane A2 mimic), 5 μM ADP-, 100 μM arachidonic acid-(AA), 10 μg/ml collagen-, and 0.1 U/ml thrombin-induced platelet aggregation in a concentration-dependent manner with IC50 of 2.1 μM, 4.1 μM, 5.8 μM, 7.0 μM, and 12.7 μM, respectively. MNS inhibits platelet aggregation caused by either the calcium ionophore A23187 (1 μM) or the protein kinase C (PKC) activator PDBu (200 nM) with IC50 of 25.9 μM and 4.8 μM, respectively. MNS (20 μM) decreases dthrombin-induced P-selectin expression on platelets to levels comparable to those observed in PGE1-treated platelets. MNS (20 μM) markedly inhibits thrombin-but not PDBu-induced MARCKS phosphorylation in platelets. MNS (20 μM) markedly inhibits protein tyrosine phosphorylation at either 0.5 min or 3 min after thrombin or collagen stimulation in platelets. [1] MNS stimulates UbG76V-GFP and ODD-Luc degradation with IC50 of 1.6 μM and 5.9 μM, respectively. MNS inhibits MG132-induced accumulation of the reporter with IC50 of 2.1 μM. [2] MNS inhibits Gram-positive (Staphylococcus aureus and Enterococcus faecalis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria with minimum inhibitory concentrations (MICs) of 128 mg/L. [3] MNS is much more potent than genistein in inhibiting platelet aggregation and protein tyrosine phosphorylation. MNS (3,4-Methylenedioxy-β-nitrostyrene) is equally potent as inhibitors of platelet aggregation as 3,4-dimethoxy-β-nitrostyrene. MNS (20 μM) concentration-dependently prevents ATP release from platelets stimulated by thrombin or collagen. MNS (20 μM) inhibits thrombin-induced PAC-1 binding to human platelets. [4]

Protocol

Solubility (25°C)

In vitro DMSO 39 mg/mL (201.9 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 193.16
Formula

C9H7NO4

CAS No. 1485-00-3
Storage powder
in solvent
Synonyms MDBN
Smiles C1OC2=C(O1)C=C(C=C2)C=C[N+](=O)[O-]

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04473027 Not yet recruiting Diagnostic Test: Blood sample collection Melanoma|Cancer University Hospital Ghent|Jessa Hospital|University Hospital Antwerp|Algemeen Ziekenhuis Maria Middelares|GZA Ziekenhuizen Campus Sint-Augustinus|AZ Sint-Jan AV|AZ Nikolaas|AZ Sint-Lucas Gent|AZ Sint-Lucas Brugge|General Hospital Groeninge|OLV van Lourdes Hospital Waregem|AZ Damiaan|AZ Delta|ASZ Aalst|Belgian Red Cross August 1 2020 --
NCT01362244 Completed Drug: Mepolizumab|Drug: Placebo|Drug: Run In Medication Nasal Polyps GlaxoSmithKline May 12 2009 Phase 2
NCT00474799 Completed Drug: MNS075 Healthy Javelin Pharmaceuticals January 2007 Phase 1

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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p97 Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID