MCC950(CP-456773)

Catalog No.S7809 Synonyms: CP-456773

MCC950(CP-456773) Chemical Structure

Molecular Weight(MW): 426.46

MCC950 sodium salt is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.

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Cited by 9 Publications

4 Customer Reviews

  • A-C, Expression of caspase 1 and its active form caspase 1-p20, as demonstrated in representative Western blots (top) and the relative expression levels (B and C) in each group.

    ARTHRITIS & RHEUMATOLOGY, 2017, 69(8):1636-1646. MCC950(CP-456773) purchased from Selleck.

    Cells were pretreated with the indicated concentrations of MCC950 followed by incubation with APAP (2mM) for additional 18 h. Cell viability was determined by MTS assay. The data are presented as the fold change compared with APAP-treated cells and expressed as mean ± SEM (n = 3).

    Biochem Pharmacol, 2018, 154:278-292. MCC950(CP-456773) purchased from Selleck.

  • Representative lung tissue sections stained by HE with or without Mcc950 treatment.

    Biochem Biophys Res Commun, 2018, 503(4):3031-3037. MCC950(CP-456773) purchased from Selleck.

    a, b Effects of varying MCC950 concentrations (0,1,10,50 and 100 μmol/L) on NLRP3 and Caspase-1 expression within the high glucose group. The protein was measured by western blotting.GAPDH was used as an internal loading control. NG:normal control group,HG:high glucose group.Data are presented in mean ± SD, □P < 0. 05 versus normal control group, △P < 0. 05 versus high glucose group.

    BMC Complement Altern Med, 2018, 18(1):192. MCC950(CP-456773) purchased from Selleck.

Purity & Quality Control

Choose Selective Immunology & Inflammation related Inhibitors

Biological Activity

Description MCC950 sodium salt is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.
Targets
NLRP3 [1]
(in BMDM (bone marrow derived macrophages))
7.5nM
In vitro

MCC950 inhibits canonical and noncanonical NLRP3 activation in BMDM and HMDM cells at IC50 of 7.5 and 8.1nM. MCC950 specifically inhibits activation of NLRP3 but not the AIM2, NLRC4 or NLRP1 inflammasomes.[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse BMDM cells NGf2b5VHfW6ldHnvckBie3OjeR?= MXizNEBucW6| NVjBfWRPUW6qaXLpeIlwdiCxZjDOUHJROyCrbjDtc5V{\SCETVTNJINmdGy|IIDy[Ylv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDBWHAh[WSmaYTpc44h[nliRVzJV2Eh[W6jbInzbZMvKEmFNUC9NE4xODd3IN88US=> M1\Be|I3PDJ{MEC2
mouse BMDM cells M1ThcmZ2dmO2aX;uJIF{e2G7 MUKzNEBucW6| NXzaUXNrUW6qaXLpeIlwdiCxZjDOUHJROyCrbn\sZY1u[XOxbXWgZYN1cX[jdHnvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDCUWROKGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBPVFKSMzDpcoZt[W2vYYPvcYUucW6mdXPl[EBKVC1zYnX0ZUBxem:mdXP0bY9vKHC{ZXnuZ5Vj[XSnZDDmc5IhOzBibXnud{Bnd2yub4fl[EBjgSCQTGLQN{B{fGmvdXzheIlwdiC5aYToJGFVWCCkeTDFUGlUSS5iSVO1NF0xNjByN{Wg{txO M{PONlI4QTl2N{Oz
INS-1 cells NHjyPZpHfW6ldHnvckBie3OjeR?= M364cVAvODFizszN NHr2Z3QyKGh? NXzrOpBrVUOFOUWwJIlvcGmkaYTl[EBvd3Rib37sfUBqdm[uYX3tZZNwdWViYXP0bZZifGmxbjDieZQh[Wy|bzDBcochUUlvaX7keYNm\CCLTD2x{tIh\WyndnH0bY9vKGGwZDDj[YxtKGGyb4D0c5NqeyCrbjDJUnMuOSClZXzsd{4> MnvnN|A6OzlzOUK=
16HBE cells Mo\sR4VtdCC4aXHibYxqfHliYYPzZZk> NFPZNngxNjBzLDCwMlEtKDFuIEGwMEAyODBizszN MorTNlQtKDR6LDC3Nog> NVLMbG1lOTByzszNJG1ESzl3MDDmeY5kfGmxbnHscJkh[WyuZY\pZZRm\CC|aXzpZ4EucW6mdXPl[EBi[3SrdnH0bY9vKG:oIF7MVnA{KGmwZnzhcY1ie2:vZTDpckAyPiCKQlWgZ4VtdHNw M3zQUVI6OjV6N{S2
THP-1 monocytes M{\mZWZ2dmO2aX;uJIF{e2G7 NUfndYl{OSEQvF2= NFPPdII{OCCvaX7z NXiybWp3WHKndILlZZRu\W62IH;mJHRJWOLCkEGgcY9vd2O7dHXzJIZweiB|MDDtbY4hf2m2aDD0bIl{KGOxbYDveY5lKHKnc4XseIVlKGmwIHGgd4lodmmoaXPhcpQhemWmdXP0bY9vKGmwIFnM5qCROc7{IILlcIVie2VuIHL1eEBvd3RicInyc5B1d3OrczD1dI9vKHS{YX7z[oVkfGmxbjD3bZRpKEySUz6= NH3BPIozPjF5M{m4PC=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NLRP3 / Casp-1 / BMI1 / ALDH1 / CD44; 

PubMed: 28865486     


The expression of NLRP3 inflammasome, BMI1, ALDH1 and CD44 was analyzed by Western blot after treatment with LPS + ATP (6 h) and MCC950(12 h) in CAL27.

28865486
Growth inhibition assay
Cell viability; 

PubMed: 27670879     


Cell viability from unprimed WT and Card9−/− BMDMs infected with S. Typhimurium at m.o.i. 10 for 2 and 6 h in presence or absence of NLRP3 inhibitors MCC950.

27670879
Immunofluorescence
ALDH1 / BIM1 / CD44; 

PubMed: 28865486     


CAL27 cells were treated with LPS + ATP(6 h) and 10, 25, and 50 nM MCC950 (12 h). The cells were analyzed by immunofluorescence cytochemistry. ALDH1 (green), BMI1 and CD44 (red), DAPI (blue); Scale bar = 50 μm.

28865486
ELISA
IL-1β; 

PubMed: 28726778     


Concentration-dependent effect of Mcc950 on HRECs in 30 mM glucose condition for 72 h. Data are shown as the mean±S.E.M., n=3 technical replicates. One-way ANOVA followed by Tukey's multiple comparison test. *P<0.05, #P<0.01 versus control.

28726778
In vivo MCC950 reduces interleukin-1β (IL-1β) production and attenuates the severity of experimental autoimmune encephalomyelitis (EAE). Furthermore, MCC950 treatment rescues neonatal lethality in a mouse model of CAPS and is active in ex vivo samples from individuals with Muckle-Wells syndrome. [1]

Protocol

Animal Research:

[1]

+ Expand
  • Animal Models: C57BL/6 mice
  • Formulation: DMSO/PBS
  • Dosages: 50 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Water 85 mg/mL (199.31 mM)
Ethanol 40 mg/mL (93.79 mM)
DMSO Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 426.46
Formula

C20H23N2O5S.Na

CAS No. 256373-96-3
Storage powder
in solvent
Synonyms CP-456773

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID