MCC950 Sodium (CP-456773 Sodium)

Catalog No.S7809 Synonyms: CRID3 sodium salt

For research use only.

MCC950 Sodium (CP-456773 Sodium, CRID3 sodium salt) is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.

MCC950 Sodium (CP-456773 Sodium) Chemical Structure

CAS No. 256373-96-3

Selleck's MCC950 Sodium (CP-456773 Sodium) has been cited by 118 publications

Purity & Quality Control

Choose Selective NLRP3 Inhibitors

Biological Activity

Description MCC950 Sodium (CP-456773 Sodium, CRID3 sodium salt) is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.
Targets
NLRP3 [1]
(in BMDM (bone marrow derived macrophages))
NLRP3 [1]
(in HMDM)
7.5nM 8.1 nM
In vitro

MCC950 inhibits canonical and noncanonical NLRP3 activation in BMDM and HMDM cells at IC50 of 7.5 and 8.1nM. MCC950 specifically inhibits activation of NLRP3 but not the AIM2, NLRC4 or NLRP1 inflammasomes.[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse BMDM cells MX\GeY5kfGmxbjDhd5NigQ>? M1jNeFMxKG2rboO= M1\ZSWlvcGmkaYTpc44hd2ZiTlzSVFMhcW5ibX;1d4UhSk2GTTDj[YxteyCycnXpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh\m:ubH;3[YQh[nliQWTQJIFl\Gm2aX;uJIJ6KEWOSWPBJIFv[Wy7c3nzMkBKSzVyPUCuNFA4PSEQvF2= MmDMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MkKwNFYoRjJ4NEKyNFA3RC:jPh?=
mouse BMDM cells M4fadGZ2dmO2aX;uJIF{e2G7 MUOzNEBucW6| NX7FTnluUW6qaXLpeIlwdiCxZjDOUHJROyCrbn\sZY1u[XOxbXWgZYN1cX[jdHnvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDCUWROKGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBPVFKSMzDpcoZt[W2vYYPvcYUucW6mdXPl[EBKVC1zYnX0ZUBxem:mdXP0bY9vKHC{ZXnuZ5Vj[XSnZDDmc5IhOzBibXnud{Bnd2yub4fl[EBjgSCQTGLQN{B{fGmvdXzheIlwdiC5aYToJGFVWCCkeTDFUGlUSS5iSVO1NF0xNjByN{Wg{txO NFy4NJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{m5OFc{Oyd-Mke5PVQ4OzN:L3G+
INS-1 cells MkPoSpVv[3Srb36gZZN{[Xl? NV\wdm1YOC5yMTFOwG0> M37nTVEhcA>? MnnXUWNEQTVyIHnubIljcXSnZDDuc5Qhd26ueTDpcoZt[W2vYYPvcYUh[WO2aY\heIlwdiCkdYSgZYx{dyCDbnegTWkucW6mdXPl[EBKVC1zzsKg[YxmfmG2aX;uJIFv\CClZXzsJIFxd3C2b4Ppd{BqdiCLTmOtNUBk\Wyucz6= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODl|OUG5Nkc,OzB7M{mxPVI9N2F-
16HBE cells NV7aOldLS2WubDD2bYFjcWyrdImgZZN{[Xl? MX2wMlAyNCByLkGsJFEtKDFyLDCxNFAh|ryP Mn;aNlQtKDR6LDC3Nog> MYOxNFDPxE1iTVPDPVUxKG[3bnP0bY9v[WyueTDhcIxmfmmjdHXkJJNqdGmlYT3pcoR2[2WmIHHjeIl3[XSrb36gc4YhVkyUUEOgbY5ndGGvbXHzc41mKGmwIEG2JGhDTSClZXzsd{4> M1\je|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkW4O|Q3Lz5{OUK1PFc1PjxxYU6=
THP-1 monocytes MonTSpVv[3Srb36gZZN{[Xl? NX\oZoxPOSEQvF2= NWjzSmdYOzBibXnudy=> MX\QdoV1emWjdH3lcpQhd2ZiVFjQ5qCROSCvb37vZ5l1\XNiZn;yJFMxKG2rbjD3bZRpKHSqaYOgZ49ueG:3bnSgdoV{fWy2ZXSgbY4h[SC|aXfubYZq[2GwdDDy[YR2[3Srb36gbY4hUU{kgKCx{tIhemWuZXHz[Uwh[nW2IH7veEBxgXKxcITvd4l{KHWyb36geJJidnOoZXP0bY9vKHerdHigUHBUNg>? NWPh[FI{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|M6QDhpPkK2NVc{QTh6PD;hQi=>
macrophages NVvZTpVxTnWwY4Tpc44h[XO|YYm= MmHKN|AhdWmwcx?= M2npfWlvcGmkaYTpc44hd2ZiTlzSVFMhcW5iaIXtZY4hdW:wb3P5eIUh\GW{aY\l[EBu[WO{b4DoZYdmeyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hVFCVLXnu[JVk\WRiSVytNYJmfGFicILv[JVkfGmxbjDpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh\m:ubH;3[YQh[nlibnnn[ZJq[2mwIIPv[Il2dSC|YXz0JJN1cW23bHH0bY9vKG[xcjCyJIhzeyCyb4P0JGxRWyClaHHscIVv\2ViZn;yJFMhcHK|IHL5JGVNUVODLDDJR|UxKD1iMD6wNFUh|ryPLh?= MkS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|M{i5NVAoRjJ7M{O4PVExRC:jPh?=
macrophages NGLYNo1HfW6ldHnvckBie3OjeR?= MWfJcohq[mm2aX;uJI9nKE6OUmCzJIlvKGi3bXHuJI1wdm:leYTlJIRmemm4ZXSgcYFkem:yaHHn[ZMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGxRWy2rbnT1Z4VlKEmOLUHi[ZRiKHC{b3T1Z5Rqd25iYomgSWxKW0FuIFnDOVAhRSByLkCwPEDPxE1w Mm\oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|M{i5NVAoRjJ7M{O4PVExRC:jPh?=
Assay
Methods Test Index PMID
Western blot NLRP3 / Casp-1 / BMI1 / ALDH1 / CD44 28865486
Growth inhibition assay Cell viability 27670879
Immunofluorescence ALDH1 / BIM1 / CD44 28865486
ELISA IL-1β 28726778
In vivo MCC950 reduces interleukin-1β (IL-1β) production and attenuates the severity of experimental autoimmune encephalomyelitis (EAE). Furthermore, MCC950 treatment rescues neonatal lethality in a mouse model of CAPS and is active in ex vivo samples from individuals with Muckle-Wells syndrome. [1]

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: C57BL/6 mice
  • Dosages: 50 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 426.46
Formula

C20H23N2O5S.Na

CAS No. 256373-96-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)(C1=COC(=C1)S(=O)(=O)[N-]C(=O)NC2=C3CCCC3=CC4=C2CCC4)O.[Na+]

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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