MCC950 Sodium (CP-456773 Sodium)

For research use only.

Catalog No.S7809 Synonyms: CRID3 sodium salt

56 publications

MCC950 Sodium (CP-456773 Sodium) Chemical Structure

Molecular Weight(MW): 426.46

MCC950 Sodium (CP-456773 Sodium) is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.

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Selleck's MCC950 Sodium (CP-456773 Sodium) has been cited by 56 publications

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Choose Selective NLR Inhibitors

Biological Activity

Description MCC950 Sodium (CP-456773 Sodium) is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.
Targets
NLRP3 [1]
(in BMDM (bone marrow derived macrophages))
7.5nM
In vitro

MCC950 inhibits canonical and noncanonical NLRP3 activation in BMDM and HMDM cells at IC50 of 7.5 and 8.1nM. MCC950 specifically inhibits activation of NLRP3 but not the AIM2, NLRC4 or NLRP1 inflammasomes.[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse BMDM cells MYXGeY5kfGmxbjDhd5NigQ>? NFv6VXc{OCCvaX7z NUDwbnY{UW6qaXLpeIlwdiCxZjDOUHJROyCrbjDtc5V{\SCETVTNJINmdGy|IIDy[Ylv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDBWHAh[WSmaYTpc44h[nliRVzJV2Eh[W6jbInzbZMvKEmFNUC9NE4xODd3IN88US=> MkjKNlY1OjJyME[=
mouse BMDM cells M1TY[2Z2dmO2aX;uJIF{e2G7 MXqzNEBucW6| NWXtWnFwUW6qaXLpeIlwdiCxZjDOUHJROyCrbn\sZY1u[XOxbXWgZYN1cX[jdHnvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDCUWROKGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBPVFKSMzDpcoZt[W2vYYPvcYUucW6mdXPl[EBKVC1zYnX0ZUBxem:mdXP0bY9vKHC{ZXnuZ5Vj[XSnZDDmc5IhOzBibXnud{Bnd2yub4fl[EBjgSCQTGLQN{B{fGmvdXzheIlwdiC5aYToJGFVWCCkeTDFUGlUSS5iSVO1NF0xNjByN{Wg{txO NUfBeJQ6Ojd7OUS3N|M>
INS-1 cells M3TKOGZ2dmO2aX;uJIF{e2G7 M{LENlAvODFizszN NVHSfHNMOSCq MoLhUWNEQTVyIHnubIljcXSnZDDuc5Qhd26ueTDpcoZt[W2vYYPvcYUh[WO2aY\heIlwdiCkdYSgZYx{dyCDbnegTWkucW6mdXPl[EBKVC1zzsKg[YxmfmG2aX;uJIFv\CClZXzsJIFxd3C2b4Ppd{BqdiCLTmOtNUBk\Wyucz6= Mm\YN|A6OzlzOUK=
16HBE cells NX;kW5NKS2WubDD2bYFjcWyrdImgZZN{[Xl? M3LCTFAvODFuIECuNUwhOSxiMUCsJFExOCEQvF2= MnXTNlQtKDR6LDC3Nog> NHTFXngyODEQvF2gUWNEQTVyIH\1coN1cW:wYXzsfUBidGyndnnheIVlKHOrbHnjZU1qdmS3Y3XkJIFkfGm4YYTpc44hd2ZiTlzSVFMhcW6obHHtcYF{d22nIHnuJFE3KEiERTDj[Yxtey5? Mlv0NlkzPTh5NE[=
THP-1 monocytes MkD4SpVv[3Srb36gZZN{[Xl? NYXyU|hKOSEQvF2= NHHrTGc{OCCvaX7z M3nzPXBz\XS{ZXH0cYVvfCCxZjDUTHDjiJBzIH3vco9kgXSnczDmc5IhOzBibXnuJJdqfGhidHjpd{Bkd22yb4Xu[EBz\XO3bITl[EBqdiCjIIPp[45q\mmlYX70JJJm\HWldHnvckBqdiCLTPMAlFHPuiC{ZXzlZZNmNCCkdYSgco91KHC7cn;weI9{cXNidYDvckB1emGwc3\lZ5Rqd25id3n0bEBNWFNw MWqyOlE4Ozl6OB?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NLRP3 / Casp-1 / BMI1 / ALDH1 / CD44; 

PubMed: 28865486     


The expression of NLRP3 inflammasome, BMI1, ALDH1 and CD44 was analyzed by Western blot after treatment with LPS + ATP (6 h) and MCC950(12 h) in CAL27.

28865486
Growth inhibition assay
Cell viability; 

PubMed: 27670879     


Cell viability from unprimed WT and Card9−/− BMDMs infected with S. Typhimurium at m.o.i. 10 for 2 and 6 h in presence or absence of NLRP3 inhibitors MCC950.

27670879
Immunofluorescence
ALDH1 / BIM1 / CD44; 

PubMed: 28865486     


CAL27 cells were treated with LPS + ATP(6 h) and 10, 25, and 50 nM MCC950 (12 h). The cells were analyzed by immunofluorescence cytochemistry. ALDH1 (green), BMI1 and CD44 (red), DAPI (blue); Scale bar = 50 μm.

28865486
ELISA
IL-1β; 

PubMed: 28726778     


Concentration-dependent effect of Mcc950 on HRECs in 30 mM glucose condition for 72 h. Data are shown as the mean±S.E.M., n=3 technical replicates. One-way ANOVA followed by Tukey's multiple comparison test. *P<0.05, #P<0.01 versus control.

28726778
In vivo MCC950 reduces interleukin-1β (IL-1β) production and attenuates the severity of experimental autoimmune encephalomyelitis (EAE). Furthermore, MCC950 treatment rescues neonatal lethality in a mouse model of CAPS and is active in ex vivo samples from individuals with Muckle-Wells syndrome. [1]

Protocol

Animal Research:

[1]

- Collapse
  • Animal Models: C57BL/6 mice
  • Dosages: 50 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Water 85 mg/mL (199.31 mM)
DMSO Insoluble
Ethanol '40 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 426.46
Formula

C20H23N2O5S.Na

CAS No. 256373-96-3
Storage powder
in solvent
Synonyms CRID3 sodium salt
Smiles CC(C)(O)C1=COC(=C1)[S](=O)(=O)N([Na])C(=O)NC2=C3CCCC3=CC4=C2CCC4

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID