Home Immunology & Inflammation NLRP3 inhibitor NLRP3 Inflammasome Inhibitor I For research use only.

NLRP3 Inflammasome Inhibitor I

NLRP3 Inflammasome Inhibitor I, an intermediate substrate in the synthesis of glyburide which is free of the cyclohexylurea moiety involved in insulin release, is a novel inhibitor of the NLRP3 inflammasome.

NLRP3 Inflammasome Inhibitor I Chemical Structure

NLRP3 Inflammasome Inhibitor I Chemical Structure

CAS: 16673-34-0

Selleck's NLRP3 Inflammasome Inhibitor I has been cited by 8 publications

Purity & Quality Control

Batch: S368001 DMSO] 73 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 98.94%
98.94

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Biological Activity

Description NLRP3 Inflammasome Inhibitor I, an intermediate substrate in the synthesis of glyburide which is free of the cyclohexylurea moiety involved in insulin release, is a novel inhibitor of the NLRP3 inflammasome.
Targets
NLRP3 inflammasome [1]
In vitro
In vitro

NLRP3 Inflammasome Inhibitor I inhibits the formation of the NLRP3 inflammasome in cardiomyocytes without affecting glucose metabolism.[1]
Cell Research Cell lines J774A.1 cells, HL-1 cells
Concentrations 400μM
Incubation Time 30 min
Method

Cells are cultured in a Claycomb medium and then primed with LPS (25 ng/mL) for 2 h and treated with ATP (5 mM) for 1 hour, to induce NLRP3 inflammasome formation. Cells are then treated with 16673-34-0 (400μM) or glyburide (400μM) during the LPS priming phase and then treated with ATP. The formation of the NLRP3 inflammasome is determined and quantified by ASC aggregation, caspase-1 activity, and cell death.
In Vivo
In vivo

Treatment with 16673-34-0 in a model of acute myocardial infarction because of ischemia and reperfusion significantly inhibites the activity of inflammasome (caspase-1) in the heart by 90% (P < 0.01) and reduces infarct size, measured at pathology (by >40%, P < 0.01) and with troponin I levels (by >70%, P < 0.01).[1]

Chemical Information & Solubility

Molecular Weight 368.84 Formula

C16H17ClN2O4S
CAS No. 16673-34-0 SDF Download NLRP3 Inflammasome Inhibitor I SDF
Smiles COC1=C(C=C(C=C1)Cl)C(=O)NCCC2=CC=C(C=C2)S(=O)(=O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 73 mg/mL ( (197.91 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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