MCC950 Sodium

Catalog No.S7809 Batch:S780906

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Technical Data

Formula

C20H23N2O5S.Na
Molecular Weight 426.46 CAS No. 256373-96-3
Solubility (25°C)* In vitro Water 85 mg/mL (199.31 mM)
Ethanol 85 mg/mL (199.31 mM)
DMSO Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description MCC950 Sodium is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.
Targets
NLRP3 [1]
(in BMDM (bone marrow derived macrophages))		 NLRP3 [1]
(in HMDM)
7.5nM 8.1 nM
In vitro

MCC950 inhibits canonical and noncanonical NLRP3 activation in BMDM and HMDM cells at IC50 of 7.5 and 8.1nM. MCC950 specifically inhibits activation of NLRP3 but not the AIM2, NLRC4 or NLRP1 inflammasomes.[1]
In vivo

MCC950 reduces interleukin-1β (IL-1β) production and attenuates the severity of experimental autoimmune encephalomyelitis (EAE). Furthermore, MCC950 treatment rescues neonatal lethality in a mouse model of CAPS and is active in ex vivo samples from individuals with Muckle-Wells syndrome. [1]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    H9c2 cardiomyocyte cells

  • Concentrations

    7 nM

  • Incubation Time

    24 h

  • Method

    Cardiomyocytes were treated with NLRP3 inhibitor MCC950 for 24 h.
Animal Study:

[1]
  • Animal Models

    C57BL/6 mice

  • Dosages

    50 mg/kg

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/25686105/
  • https://pubmed.ncbi.nlm.nih.gov/35013106/

Customer Product Validation

A-C, Expression of caspase 1 and its active form caspase 1-p20, as demonstrated in representative Western blots (top) and the relative expression levels (B and C) in each group.

Data from [ , , ARTHRITIS & RHEUMATOLOGY, 2017, 69(8):1636-1646 ]

Cells were pretreated with the indicated concentrations of MCC950 followed by incubation with APAP (2mM) for additional 18 h. Cell viability was determined by MTS assay. The data are presented as the fold change compared with APAP-treated cells and expressed as mean ± SEM (n = 3).

Data from [ , , Biochem Pharmacol, 2018, 154:278-292 ]

Representative lung tissue sections stained by HE with or without Mcc950 treatment.

Data from [ , , Biochem Biophys Res Commun, 2018, 503(4):3031-3037 ]

a, b Effects of varying MCC950 concentrations (0,1,10,50 and 100 μmol/L) on NLRP3 and Caspase-1 expression within the high glucose group. The protein was measured by western blotting.GAPDH was used as an internal loading control. NG:normal control group,HG:high glucose group.Data are presented in mean ± SD, □P < 0. 05 versus normal control group, △P < 0. 05 versus high glucose group.

Data from [ , , BMC Complement Altern Med, 2018, 18(1):192 ]

Selleck's MCC950 Sodium Has Been Cited by 168 Publications

Discovery of potent and selective inhibitors of human NLRP3 with a novel mechanism of action [ J Exp Med, 2025, 222(11)e20242403] PubMed: 40892070
LXR pathway drives hormonal response intensity in polycystic ovary syndrome [ EMBO Mol Med, 2025, 10.1038/s44321-025-00251-1] PubMed: 40399491
Peroxiredoxin 1 promotes intestinal inflammation by activating the NLRP3 inflammasome in macrophages through lysosomal disruption in Crohn's disease [ Cell Death Dis, 2025, 16(1):565] PubMed: 40715057
Co-exposure to polystyrene nanoplastics and glyphosate exacerbates NETs-mediated pyroptosis by activating the NLRP3 inflammasome in mouse liver [ Ecotoxicol Environ Saf, 2025, 302:118529] PubMed: 40554107
PRRSV-2 nsp2 Ignites NLRP3 inflammasome through IKKβ-dependent dispersed trans-Golgi network translocation [ PLoS Pathog, 2025, 21(1):e1012915] PubMed: 39869629
Effects of acute pro-inflammatory stimulation and 25-hydroxycholesterol on hippocampal plasticity and learning involve NLRP3 inflammasome and cellular stress responses [ Sci Rep, 2025, 15(1):6149] PubMed: 39979396
Experimental TET2 Clonal Hematopoiesis Predisposes to Renal Hypertension Through an Inflammasome-Mediated Mechanism [ Circ Res, 2024, 135(9):933-950] PubMed: 39234670
Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome [ Bone Res, 2024, 12(1):53] PubMed: 39242551
Fenebrutinib, a Bruton's tyrosine kinase inhibitor, blocks distinct human microglial signaling pathways [ J Neuroinflammation, 2024, 21(1):276] PubMed: 39465429
P2X7R Modulates NEK7-NLRP3 Interaction to Exacerbate Experimental Autoimmune Prostatitis via GSDMD-mediated Prostate Epithelial Cell Pyroptosis [ Int J Biol Sci, 2024, 20(9):3393-3411] PubMed: 38993566

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.