Lovastatin

For research use only.

Catalog No.S2061 Synonyms: MK-803

18 publications

Lovastatin Chemical Structure

Molecular Weight(MW): 404.54

Lovastatin is an inhibitor of HMG-CoA reductase with IC50 of 3.4 nM in a cell-free assay, used for lowering cholesterol (hypolipidemic agent).

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 90 In stock
USD 70 In stock
USD 170 In stock
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Selleck's Lovastatin has been cited by 18 publications

2 Customer Reviews

  • To evaluate the role of cell cycle progression in androgen induced DSB formation, LAPC4 cells were either treated with solvent control or 50 μM lovastatin for 36 h prior to stimulation with 100 nM DHT for 6 h. Note that androgen induced γH2A.X foci formation was not different between control and lovastatin pre-treated cells.

    Clin Cancer Res, 2016, 22(13):3310-3319. Lovastatin purchased from Selleck.

  • The efficiency of siRNA-mediated target gene knockdown was shown by Western Blot analysis.

    Cell Cycle, 2018, 17(9):1138-1145. Lovastatin purchased from Selleck.

Purity & Quality Control

Choose Selective HMG-CoA Reductase Inhibitors

Biological Activity

Description Lovastatin is an inhibitor of HMG-CoA reductase with IC50 of 3.4 nM in a cell-free assay, used for lowering cholesterol (hypolipidemic agent).
Targets
HMG-CoA reductase [1]
(Cell-free assay)
3.4 nM
In vitro

Lovastatin inhibits LPS- and cytokine-mediated production of NO and expression of iNOS in rat primary astrocytes. Lovastatin inhibits LPS-induced expression of TNF-alpha, IL-1beta, and IL-6 in rat primary astrocytes, microglia, and macrophages. [1] Lovastatin results in over 95% inhibition of DNA synthesis as measured by incorporation of [3H]thymidine into DNA. Lovastatin synchronizes cells in the G1 and not in the G0 phase of the cell cycle. Lovastatin has a similar growth-inhibitory activity against ras-dependent as well as ras-independent cell lines. [2] Lovastatin produces a profound reduction of apolipoprotein-B-containing lipoproteins, especially LDL cholesterol and, to a lesser extent, plasma triglyc- erides, and a small increase in HDL cholesterol. [3] Lovastatin arrests cells by inhibiting the proteasome, which results in the accumulation of p21 and p27, leading to G1 arrest. Lovastatin is an inhibitor of hydroxymethyl glutaryl (HMG)-CoA reductase, the rate-limiting enzyme in cholesterol synthesis. Lovastatin can be used to arrest cultured cells in the G1 phase of the cell cycle, resulting in the stabilization of the cyclin-dependent kinase inhibitors (CKIs) p21 and p27. [4] Lovastatin (2-10 mM) arrests cells in G1 and also prolonged--or arrested a minor fraction of cells in--the G2 phase of the cell cycle in human bladder carcinoma T24 cell line expressing activated p21ras. Lovastatin (50 mM) is cytotoxic in human bladder carcinoma T24 cell line expressing activated p21ras. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEP G2 cells MkTiSpVv[3Srb36gZZN{[Xl? NWn5O|NXUW6qaXLpeIlwdiCxZjDj[YxtfWyjcjDIUWcuS2:DIILl[JVkfGG|ZTDpckBkfWy2dYLld{Bw\iCqZYDheIlkKGOnbHzzJEhJTVBiR{KsJIEhcHWvYX6gbIVx[XSxbXGgZ4VtdCCuaX7lLUwhUUN3ME21[U0xPSEQvF2= NV70[IdQOjF3M{KxNy=>
HES 9 cell line MmTkSpVv[3Srb36gZZN{[Xl? M4HZ[WNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDDIUWcuS2:DIILl[JVkfGG|ZTDifUA2OCVid3HzJIRmfGW{bXnu[YQhcW5iSFXTJFkh[2WubDDsbY5mNCCLQ{WwQVAvODF|IN88US=> M36yb|E2Ojd5OUG=
HEP G2 cells MnXSSpVv[3Srb36gZZN{[Xl? M1jtUnRme3SnZDDmc5IhcW6qaXLpeIlwdiCxZjDjbI9t\XO2ZYLvcEBjcW:|eX70bIV{cXNiaX6gTGVRKEd{IHPlcIx{NCCLQ{WwQVAvODJ7IN88US=> MU[3PVMzPTVz
human SW480 cells M1PzXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV65OkBp MYfHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTW|Q5OCClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRUKGG|c3H5MEBKSzVyPUeuNUDPxE1? MW[xO|Q4Ojl4Mh?=
human HepG2 cells MnfCSpVv[3Srb36gZZN{[Xl? MVG2JIg> MVzMbZBq\CCub4fldolv\yCjY4Tpeol1gSCrbjDoeY1idiCKZYDHNkBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCxbHXpZ{Bi[2mmIHXsbYNqfGWmIHzpdIllKGGlY4XteYxifGmxbjDh[pRmeiB4IHjyd{BjgSCxaXytdoVlKE9ic4ThbY5qdmdibXX0bI9lNCCLQ{WwQVgvOyEQvF2= M2HsSVI3OTZ7MUK1
human A549 cells MX7DfZRwfG:6aXRCpIF{e2G7 MXO3NkBp M1jC[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNU41KM7:TR?= M4mzSlI{PTdyNUSy
human HS68 cells MkfzR5l1d3SxeHnjxsBie3OjeR?= M1rhd|czKGh? MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIV|Y5KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkOuNkDPxE1? NUDSNm9JOjN3N{C1OFI>
mouse MEF cells MX7DfZRwfG:6aXRCpIF{e2G7 M4LiTFczKGh? M4CxTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJG1GTiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUO1JO69VQ>? MWGyN|U4ODV2Mh?=
human HT29 cells MoLFS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NED2dpg6PiCq NHf6VY9Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJXDJ7IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFNiYYPzZZktKEmFNUC9OFYvQCEQvF2= NILOXIcyPzR5Mkm2Ni=>
human SW480 cells NFjsdWVHfW6ldHnvckBie3OjeR?= NWXvO5o3OjBizszN MlzFTY5pcWKrdHnvckBw\iCIQmOtd5RqdXWuYYTl[EBqdmO{ZXHz[UBqdiCDa4SgdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIGPXOFgxKGOnbHzzJIF1KDJyIIXNJIJ6KGmvbYXuc4Jtd3RiYX7hcJl{cXN? MnfWNVc1PzJ7NkK=
human RPMI8226 cells M4G0fGFxd3C2b4Ppd{Bie3OjeR?= MlHSNlAh|ryP NGeyPWc1QCCq NVWyXZBjUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDSVG1KQDJ{NjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBk[XOyYYPlMVMh[2ynYY\h[4Uh[XRiMkCgeW0hcW6ldXLheIVlKG[xcjC0PEBpenNiYomgbY1ufW6xYnzveEBu\XSqb3S= MmXqNlU6OzV4NEO=
human MDA-MB-468 cells MV;GeY5kfGmxbjDhd5NigQ>? MnziOFghcA>? NIfmfXJVd3SjbDDndo94fGhiaX7obYJqfGmxbjDv[kBRWixiRWKsJGhGWjJvbnXnZZRqfmViaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{Bi\nSncjC0PEBpenNiYomgW3NVNTFiYYPzZZk> NWHaZllsOjR3NU[1NFQ>
human MDA-MB-361 cells  MlrnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUm0PEBp NUXrUnNLT3Kxd4ToJIlvcGmkaYTpc44hd2ZiRWKtdI9{cXSrdnWsJGhGWjJvcH;zbZRqfmViaIXtZY4hVUSDLV3CMVM3OSClZXzsd{Bi\nSncjC0PEBpenNiYomgW3NVNTFiYYPzZZk> M1;YVlI1PTV4NUC0
human AU565 cells MlTOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFvTbFc1QCCq MYDHdo94fGhiaX7obYJqfGmxbjDv[kBGWi2wZXfheIl3\SxiSFXSNk1xd3OrdHn2[UBpfW2jbjDBWVU3PSClZXzsd{Bi\nSncjC0PEBpenNiYomgW3NVNTFiYYPzZZk> MoLUNlQ2PTZ3MES=
human MCF7 cells M{n2bGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mo\VOFghcA>? Mlr3WI91[WxiZ4Lve5RpKGmwaHnibZRqd25ib3[gSXIueG:|aYTpeoUtKEiHUkKtcoVo[XSrdnWgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliV2PUMVEh[XO|YYm= NFuyO3czPDV3NkWwOC=>
human MDA-MB-231 cells MWDGeY5kfGmxbjDhd5NigQ>? NVuxdFZ{OjRiaB?= NUnCNnlQUW6mdXP0bY9vKG:oIICyNUBmgHC{ZYPzbY9vKGmwIHj1cYFvKFCULDDFVkwhUEWUMj3u[YdifGm4ZTDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG2IEGgeI8hOTBidV2gZYZ1\XJiMkSgbJJ{KGK7IIfld5Rmem5iYnzveEBidmGueYPpdy=> MnWzNlQ2PTZ3MES=
human LS180 cells NVPVWZh2TnWwY4Tpc44h[XO|YYm= NXHaeJk6OjBizszN NVPMPFJjUW6qaXLpeIlwdiCxZjDzeZJ3cX[rbjDlfJBz\XO|aX;uJIlvKHCjcnXueEBpfW2jbjDMV|E5OCClZXzsd{BifCB{MDD1UUBjgSCrbX31co9jdG:2IHHuZYx6e2m| NHLQRZAyPzR5Mkm2Ni=>
human K562 cells MkDZSpVv[3Srb36gZZN{[Xl? MUW0PEBp MVTJcohq[mm2aX;uJI9nKEeJVHHz[VEhcW5iaIXtZY4hUzV4MjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YhWmGyMXGgdJJwfGWrbjDn[ZJidnmuZ3XyZY56dGG2aX;uJIFnfGW{IES4JIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6p NFrQSG8zODh|MkOyOi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT / AKT / p-GSK3β / GSK3β / p-β-catenin / β-catenin / TAZ ; 

PubMed: 30975976     


Western blot assays of RKO cells treated with lovastatin or lovastatin plus MVA. RKO or SW480 cells are treated with lovastatin (5 μM) or lovastatin plus MVA (0.5 mM) for 48 h, then the protein is isolated for the western blot assay. 

HMGR; 

PubMed: 17412884     


JJN-3 cells were treated for 24 h with 0–5 μM LOV. HMG-CoA reductase (HMGR) was detected by SDS-polyacrylamide gel electrophoresis and immunoblotting. GAPDH served as loading control.

30975976 17412884
Immunofluorescence
β-catenin; 

PubMed: 30975976     


Lovastatin treatment decreased the protein level of β-catenin in SW480 cells. Cells are treated with lovastatin (5 μM) or lovastatin plus MVA (0.5 mM) for 48 h, then immunofluorescence is performed to detect the β-catenin distribution

30975976
Growth inhibition assay
Cell viability ; 

PubMed: 20205716     


Cell proliferation of human breast cancer cell lines. (a) MDAMB231 and (b) MDAMB468. Cells were treated with increasing concentrations of lovastatin lactone or lovastatin acid (μg/mL) for 48 hours. Data are presented as mean ± standard deviation (n = 5) *P < 0.05;**P < 0.05; ***P < 0.001.

20205716

Protocol

Solubility (25°C)

In vitro DMSO 8 mg/mL (19.77 mM)
Ethanol 6 mg/mL (14.83 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 404.54
Formula

C24H36O5

CAS No. 75330-75-5
Storage powder
in solvent
Synonyms MK-803
Smiles CCC(C)C(=O)OC1CC(C)C=C2C=CC(C)C(CCC3CC(O)CC(=O)O3)C12

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01478828 Terminated Drug: Lovastatin Prostate Cancer Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins|Patrick C Walsh Prostate Cancer Research Fund July 13 2012 Not Applicable
NCT01527669 Completed Drug: LipoCol Forte capsules|Drug: Lovastatin Tablet Healthy Subjects National Taiwan University Hospital|National Science Council Taiwan February 2012 Phase 4
NCT01385020 Completed Drug: Gemfibrozil & red yeast rice (LipoCol) Healthy Subjects National Taiwan University Hospital|National Science Council Taiwan July 2011 Phase 4
NCT00700921 Completed Drug: Lovastatin|Drug: Placebo Chronic Obstructive Pulmonary Disease (COPD) National Jewish Health|National Heart Lung and Blood Institute (NHLBI) April 2008 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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HMG-CoA Reductase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID