Lovastatin

Catalog No.S2061 Synonyms: MK-803

Lovastatin Chemical Structure

Molecular Weight(MW): 404.54

Lovastatin is an inhibitor of HMG-CoA reductase with IC50 of 3.4 nM in a cell-free assay, used for lowering cholesterol (hypolipidemic agent).

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 70 In stock
USD 170 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 8 Publications

2 Customer Reviews

  • To evaluate the role of cell cycle progression in androgen induced DSB formation, LAPC4 cells were either treated with solvent control or 50 μM lovastatin for 36 h prior to stimulation with 100 nM DHT for 6 h. Note that androgen induced γH2A.X foci formation was not different between control and lovastatin pre-treated cells.

    Clin Cancer Res, 2016, 22(13):3310-3319. Lovastatin purchased from Selleck.

    The efficiency of siRNA-mediated target gene knockdown was shown by Western Blot analysis.

    Cell Cycle, 2018, 17(9):1138-1145. Lovastatin purchased from Selleck.

Purity & Quality Control

Choose Selective HMG-CoA Reductase Inhibitors

Biological Activity

Description Lovastatin is an inhibitor of HMG-CoA reductase with IC50 of 3.4 nM in a cell-free assay, used for lowering cholesterol (hypolipidemic agent).
Targets
HMG-CoA reductase [1]
(Cell-free assay)
3.4 nM
In vitro

Lovastatin inhibits LPS- and cytokine-mediated production of NO and expression of iNOS in rat primary astrocytes. Lovastatin inhibits LPS-induced expression of TNF-alpha, IL-1beta, and IL-6 in rat primary astrocytes, microglia, and macrophages. [1] Lovastatin results in over 95% inhibition of DNA synthesis as measured by incorporation of [3H]thymidine into DNA. Lovastatin synchronizes cells in the G1 and not in the G0 phase of the cell cycle. Lovastatin has a similar growth-inhibitory activity against ras-dependent as well as ras-independent cell lines. [2] Lovastatin produces a profound reduction of apolipoprotein-B-containing lipoproteins, especially LDL cholesterol and, to a lesser extent, plasma triglyc- erides, and a small increase in HDL cholesterol. [3] Lovastatin arrests cells by inhibiting the proteasome, which results in the accumulation of p21 and p27, leading to G1 arrest. Lovastatin is an inhibitor of hydroxymethyl glutaryl (HMG)-CoA reductase, the rate-limiting enzyme in cholesterol synthesis. Lovastatin can be used to arrest cultured cells in the G1 phase of the cell cycle, resulting in the stabilization of the cyclin-dependent kinase inhibitors (CKIs) p21 and p27. [4] Lovastatin (2-10 mM) arrests cells in G1 and also prolonged--or arrested a minor fraction of cells in--the G2 phase of the cell cycle in human bladder carcinoma T24 cell line expressing activated p21ras. Lovastatin (50 mM) is cytotoxic in human bladder carcinoma T24 cell line expressing activated p21ras. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEP G2 cells NHW4d4xHfW6ldHnvckBie3OjeR?= MVXJcohq[mm2aX;uJI9nKGOnbHz1cIFzKEiPRz3Dc2EhemWmdXP0ZZNmKGmwIHP1cJR2emW|IH;mJIhmeGG2aXOgZ4VtdHNiKFjFVEBIOixiYTDoeY1idiCqZYDheI9u[SClZXzsJIxqdmVrLDDJR|UxRTWnLUC1JO69VQ>? NVTUbVFIOjF3M{KxNy=>
HES 9 cell line NHPMdHdHfW6ldHnvckBie3OjeR?= NEi3O3REd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgTG1INUOxQTDy[YR2[3Sjc3WgZpkhPTBnIIfhd{Bl\XSncn3pcoVlKGmwIFjFV{A6KGOnbHygcIlv\SxiSVO1NF0xNjBzMzFOwG0> MkTDNVUzPzd7MR?=
HEP G2 cells NIXoRppHfW6ldHnvckBie3OjeR?= MoG1WIV{fGWmIH\vdkBqdmirYnn0bY9vKG:oIHPoc4xme3Sncn;sJIJqd3O7boTo[ZNqeyCrbjDISXAhTzJiY3XscJMtKEmFNUC9NE4xOjlizszN M2G1cFc6OzJ3NUG=
human SW480 cells NFTq[45Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX65OkBp MULHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTW|Q5OCClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRUKGG|c3H5MEBKSzVyPUeuNUDPxE1? MVOxO|Q4Ojl4Mh?=
human HepG2 cells NYK4N415TnWwY4Tpc44h[XO|YYm= NFPYTJk3KGh? NIOzS4ZNcXCrZDDsc5dmemmwZzDhZ5Rqfmm2eTDpckBpfW2jbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDvcIVq[yCjY3nkJIVtcWOrdHXkJIxqeGmmIHHjZ5VufWyjdHnvckBi\nSncjC2JIhzeyCkeTDvbYwuemWmIF:gd5RicW6rbnegcYV1cG:mLDDJR|UxRThwMzFOwG0> NVz3eYE5OjZzNkmxNlU>
human A549 cells MnvBR5l1d3SxeHnjxsBie3OjeR?= MVq3NkBp MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUGuOEDPxE1? M1P0VVI{PTdyNUSy
human HS68 cells MVPDfZRwfG:6aXRCpIF{e2G7 NWfzco9jPzJiaB?= M3jqW2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhUPjhiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2yN{4zKM7:TR?= NVmzN5NROjN3N{C1OFI>
mouse MEF cells NX3Tclk6S3m2b4TvfIlkyqCjc4PhfS=> Mm\TO|IhcA>? MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDNSWYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1|NTFOwG0> NXqxWIRMOjN3N{C1OFI>
human HT29 cells MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWjrenBNQTZiaB?= NVzZ[IxXT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUFR{OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVQ3NjhizszN MXqxO|Q4Ojl4Mh?=
human SW480 cells M3\v[GZ2dmO2aX;uJIF{e2G7 MlraNlAh|ryP NGL0SYdKdmirYnn0bY9vKG:oIF\CV{1{fGmvdXzheIVlKGmwY4LlZZNmKGmwIFHreEBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gV3c1QDBiY3XscJMh[XRiMkCgeW0h[nliaX3teY5w[myxdDDhcoFtgXOrcx?= Ml7ONVc1PzJ7NkK=
human RPMI8226 cells NGXtdYVCeG:ydH;zbZMh[XO|YYm= NFO5NIIzOCEQvF2= M2jlVFQ5KGh? NHTTNWJKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIGLQUWk5OjJ4IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJINie3Cjc3WtN{BkdGWjdnHn[UBifCB{MDD1UUBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDpcY12dm:kbH;0JI1mfGixZB?= NI\6VGwzPTl|NU[0Ny=>
human MDA-MB-468 cells MXHGeY5kfGmxbjDhd5NigQ>? NH71V5k1QCCq NEfBOW9Vd3SjbDDndo94fGhiaX7obYJqfGmxbjDv[kBRWixiRWKsJGhGWjJvbnXnZZRqfmViaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{Bi\nSncjC0PEBpenNiYomgW3NVNTFiYYPzZZk> NFjCS5QzPDV3NkWwOC=>
human MDA-MB-361 cells  MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWO0PEBp NWD4S|RKT3Kxd4ToJIlvcGmkaYTpc44hd2ZiRWKtdI9{cXSrdnWsJGhGWjJvcH;zbZRqfmViaIXtZY4hVUSDLV3CMVM3OSClZXzsd{Bi\nSncjC0PEBpenNiYomgW3NVNTFiYYPzZZk> Mnr6NlQ2PTZ3MES=
human AU565 cells M3LGfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH;3OIo1QCCq NWfBOoJ3T3Kxd4ToJIlvcGmkaYTpc44hd2ZiRWKtcoVo[XSrdnWsJGhGWjJvcH;zbZRqfmViaIXtZY4hSVV3NkWgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFeVVD2xJIF{e2G7 MnHqNlQ2PTZ3MES=
human MCF7 cells M37w[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYG0PEBp M4S3d3RwfGGuIHfyc5d1cCCrbnjpZol1cW:wIH;mJGVTNXCxc3n0bZZmNCCKRWKyMY5m\2G2aY\lJIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGfTWE0yKGG|c3H5 M1jOO|I1PTV4NUC0
human MDA-MB-231 cells MoX1SpVv[3Srb36gZZN{[Xl? M37GclI1KGh? NYTmbWdYUW6mdXP0bY9vKG:oIICyNUBmgHC{ZYPzbY9vKGmwIHj1cYFvKFCULDDFVkwhUEWUMj3u[YdifGm4ZTDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG2IEGgeI8hOTBidV2gZYZ1\XJiMkSgbJJ{KGK7IIfld5Rmem5iYnzveEBidmGueYPpdy=> MoHDNlQ2PTZ3MES=
human LS180 cells NUHwTYk6TnWwY4Tpc44h[XO|YYm= MkizNlAh|ryP M1vwVmlvcGmkaYTpc44hd2Zic4Xyeol3cW5iZYjwdoV{e2mxbjDpckBx[XKnboSgbJVu[W5iTGOxPFAh[2WubIOgZZQhOjBidV2gZpkhcW2vdX7vZoxwfCCjbnHsfZNqew>? MonGNVc1PzJ7NkK=
human K562 cells MnrhSpVv[3Srb36gZZN{[Xl? Moq0OFghcA>? MU\Jcohq[mm2aX;uJI9nKEeJVHHz[VEhcW5iaIXtZY4hUzV4MjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YhWmGyMXGgdJJwfGWrbjDn[ZJidnmuZ3XyZY56dGG2aX;uJIFnfGW{IES4JIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6p NES0[FQzODh|MkOyOi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT / AKT / p-GSK3β / GSK3β / p-β-catenin / β-catenin / TAZ ; 

PubMed: 30975976     


Western blot assays of RKO cells treated with lovastatin or lovastatin plus MVA. RKO or SW480 cells are treated with lovastatin (5 μM) or lovastatin plus MVA (0.5 mM) for 48 h, then the protein is isolated for the western blot assay. 

HMGR; 

PubMed: 17412884     


JJN-3 cells were treated for 24 h with 0–5 μM LOV. HMG-CoA reductase (HMGR) was detected by SDS-polyacrylamide gel electrophoresis and immunoblotting. GAPDH served as loading control.

30975976 17412884
Immunofluorescence
β-catenin; 

PubMed: 30975976     


Lovastatin treatment decreased the protein level of β-catenin in SW480 cells. Cells are treated with lovastatin (5 μM) or lovastatin plus MVA (0.5 mM) for 48 h, then immunofluorescence is performed to detect the β-catenin distribution

30975976
Growth inhibition assay
Cell viability ; 

PubMed: 20205716     


Cell proliferation of human breast cancer cell lines. (a) MDAMB231 and (b) MDAMB468. Cells were treated with increasing concentrations of lovastatin lactone or lovastatin acid (μg/mL) for 48 hours. Data are presented as mean ± standard deviation (n = 5) *P < 0.05;**P < 0.05; ***P < 0.001.

20205716

Protocol

Solubility (25°C)

In vitro DMSO 8 mg/mL (19.77 mM)
Ethanol 6 mg/mL (14.83 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 404.54
Formula

C24H36O5

CAS No. 75330-75-5
Storage powder
in solvent
Synonyms MK-803

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01478828 Terminated Drug: Lovastatin Prostate Cancer Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins|Patrick C Walsh Prostate Cancer Research Fund July 13 2012 Not Applicable
NCT01527669 Completed Drug: LipoCol Forte capsules|Drug: Lovastatin Tablet Healthy Subjects National Taiwan University Hospital|National Science Council Taiwan February 2012 Phase 4
NCT01385020 Completed Drug: Gemfibrozil & red yeast rice (LipoCol) Healthy Subjects National Taiwan University Hospital|National Science Council Taiwan July 2011 Phase 4
NCT00700921 Completed Drug: Lovastatin|Drug: Placebo Chronic Obstructive Pulmonary Disease (COPD) National Jewish Health|National Heart Lung and Blood Institute (NHLBI) April 2008 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

HMG-CoA Reductase Signaling Pathway Map

Tags: buy Lovastatin | Lovastatin supplier | purchase Lovastatin | Lovastatin cost | Lovastatin manufacturer | order Lovastatin | Lovastatin distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID