Catalog No.S2782

GW4064 Chemical Structure

Molecular Weight(MW): 542.84

GW4064 is an agonist of farnesoid X receptor (FXR) with EC50 of 65 nM in CV1 cell line and displays no activity at other nuclear receptors at concentrations up to 1 μM.

Size Price Stock Quantity  
In DMSO USD 150 In stock
USD 70 In stock
USD 110 In stock
USD 270 In stock
USD 370 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

2 Customer Reviews

  • FXR agonist GW4064 reduced the association between β-Catenin/TCF4 complex and the TCF binding sites from Cyclin D1 promoter. ChIP analysis was carried out using antibodies against TCF4, β-Catenin, or normal IgG in lysates from Huh7 cells.

    Oncotarget, 2015, 6: 4226-38 . GW4064 purchased from Selleck.

    Immunofluorescence staining of S1PR2 (×40).

    IUBMB Life, 2016, 68(5):376-87. GW4064 purchased from Selleck.

Purity & Quality Control

Choose Selective FXR Inhibitors

Biological Activity

Description GW4064 is an agonist of farnesoid X receptor (FXR) with EC50 of 65 nM in CV1 cell line and displays no activity at other nuclear receptors at concentrations up to 1 μM.
FXR [1]
65 nM(EC50)
In vitro

GW 4064 is a full agonist with EC50 values of 80 and 90 nM, respectively, in CV-1 cells transfected with mouse and human FXR expression vectors and an established reporter gene. There is no activity of GW 4064 on other nuclear receptors, including the retinoic acid receptor, at concentrations up to 1 μM. Thus, GW 4064 is a potent and selective nonsteroidal FXR agonist. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells NXLxVWlCTnWwY4Tpc44h[XO|YYm= M4DKS2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSnhTKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUCuNFI3KM7:TR?= NI\ZPJMzPTNyNU[4PC=>
monkey CV-1 cells NVLo[2tGTnWwY4Tpc44h[XO|YYm= MmWwRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDGXHIhVEKGIHnlfJBz\XO|ZXSgbY4hdW:wa3X5JGNXNTFiY3XscJMh[XO|ZYPz[YQh[XNidILhcpNi[3SrdnH0bY9vKG:oIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCneIDy[ZN{cW:wLDDFR|UxRTBwME[1JO69VQ>? M4HueVIyOjV4MEC1
HEK293 cells Mo\GSpVv[3Srb36gZZN{[Xl? M3XISGFod26rc4TpZ{Bi[3Srdnn0fUBifCCIWGKgbY4hUEWNMkmzJINmdGy|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6NCCHQ{WwQVAvODdizszN NYfhR2toOTd{OUK2NVA>
HeLa cells MYjGeY5kfGmxbjDhd5NigQ>? MmXFNlQhcA>? M3PhWGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6g[pVtdCCuZX7neIghTliUIHX4dJJme3OnZDDpckBJ\UyjIHPlcIx{KGOxdILhcpNn\WO2ZXSge4l1cCCyU1e1MYh2dWGwIGLYVkBi\nSncjCyOEBpenNiYomgSJVidC2JbH:gcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvPTFizszN NYXPRYl2OjV7M{SyNlc>
human Caco-2 cells NFTRR5hHfW6ldHnvckBie3OjeR?= M33xfVEh|ryP NWDZVI5yPiCmYYnz NVLUWnpOSWO2aY\heIlwdiCxZjDJRmFDWCCpZX7lJIV5eHKnc4Ppc44hcW5iaIXtZY4hS2Glbz2yJINmdGy|IHH0JFEhfU1iYX\0[ZIhPiCmYYnzJIJ6KFKWLWDDVi=> M4rtVlE4QTZ|M{ex
human HepG2 cells M1vTPWZ2dmO2aX;uJIF{e2G7 NIXDXGYyKM7:TR?= MlLVNVghcA>? MmT4SIVkemWjc3WgbY4hS1mSN1GxJIdmdmViZYjwdoV{e2mxbjDpckBpfW2jbjDI[ZBIOiClZXzsd{BifCBzIIXNJIFnfGW{IEG4JIhzeyCkeTDSWE1RS1J? NHP3U5QyPzl4M{O3NS=>
human HepG2 cells M3fCSWZ2dmO2aX;uJIF{e2G7 NFW0eIwyKM7:TR?= NE\GfXUyQCCq NEniXpVF\WO{ZXHz[UBqdiCFWWC3RVEh\2WwZTDlfJBz\XO|aX;uJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG2IEGgeW0h[W[2ZYKgNVghcHK|IHL5JHJVNVCFUh?= MV2xO|k3OzN5MR?=
human Huh7 cells MV3GeY5kfGmxbjDhd5NigQ>? Mo\QNUDPxE1? MYCxPEBp NXj0TGNkSWO2aY\heIlwdiCxZjDTTHAh\2WwZTDlfJBz\XO|aX;uJIlvKGi3bXHuJGh2cDdiY3XscJMh[XRiMTD1UUBi\nSncjCxPEBpenNiYomgVnQuWEOU NUO0V5BjOTd7NkOzO|E>
human HepG2 cells MljqSpVv[3Srb36gZZN{[Xl? MoDmNUDPxE1? NHzEcGYyQCCq NIHPWXRC[3SrdnH0bY9vKG:oIFLTSXAh\2WwZTDlfJBz\XO|aX;uJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG2IEGgeW0h[W[2ZYKgNVghcHK|IHL5JHJVNVCFUh?= NFH2eIkyPzl4M{O3NS=>
human Huh7 cells NWnWfpBoTnWwY4Tpc44h[XO|YYm= NYntRpNsOSEQvF2= NIDuOIIyQCCq NILkV2dC[3SrdnH0bY9vKG:oIFLTSXAh\2WwZTDlfJBz\XO|aX;uJIlvKGi3bXHuJGh2cDdiY3XscJMh[XRiMTD1UUBi\nSncjCxPEBpenNiYomgVnQuWEOU M1nQS|E4QTZ|M{ex

... Click to View More Cell Line Experimental Data

In vivo Pharmacokinetic analysis in rats shows that GW 4064 possesses an oral bioavailability of 10% with a t1/2 = 3.5 h. Fisher rats are dosed with GW 4064 by oral gavage. After 7 days, a dose-dependent lowering of serum triglycerides is observed in the rats receiving GW 4064, with an ED50 = 20 mg/kg. [2]


Animal Research:


+ Expand
  • Animal Models: Fisher rats
  • Formulation: 0.5% methyl cellulose
  • Dosages: 0-100 mg/kg
  • Administration: Orally, b.i.d.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (184.21 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
0.5% methylcellulose
For best results, use promptly after mixing.
11 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 542.84


CAS No. 278779-30-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Related FXR Products0

Tags: buy GW4064 | GW4064 supplier | purchase GW4064 | GW4064 cost | GW4064 manufacturer | order GW4064 | GW4064 distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID