Gentamicin Sulfate

For research use only.

Catalog No.S4030 Synonyms: NSC-82261, SCH9724

Gentamicin Sulfate Chemical Structure

Molecular Weight(MW):

Gentamycin Sulfate is a broad-spectrum, aminoglycoside antibiotic used for cell culture which inhibits protein synthesis in sensitive organisms.

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Biological Activity

Description Gentamycin Sulfate is a broad-spectrum, aminoglycoside antibiotic used for cell culture which inhibits protein synthesis in sensitive organisms.
In vitro

Gentamycin Sulfate is a complex of three different closely related aminoglycoside sulfates, Gentamicins c1, c1a and c2, obtained from Micromonospora purpurea and related species. [1] They are broad-spectrum antibiotics, but may cause ear and kidney damage. Gentamycin Sulfate is reported to have activity against gram-negative bacteria, Pseudomonas aeruginosa, gram-positive Staphylococcus aureus and some mycoplasmas. Reported to have low toxicity to mammalian cells and viruses at certain concentrations, useful for long-term tissue culture studies with viruses. [2] Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. [3]


Solubility (25°C)

In vitro Water 100 mg/mL
DMSO Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight


CAS No. 1405-41-0
Storage powder
in solvent
Synonyms NSC-82261, SCH9724

In vivo Formulation Calculator (Clear solution)

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03845790 Recruiting Other: Blood and microdialysis samples Surgical Site Infection Poitiers University Hospital May 26 2019 Phase 1
NCT03632109 Terminated Drug: gentamicin 360mg IM Pharyngeal Gonococcal Infection University of Washington|National Institute of Allergy and Infectious Diseases (NIAID) September 17 2018 Phase 2|Phase 3
NCT03392909 Recruiting Drug: Gentamicin Recessive Dystrophic Epidermolysis Bullosa University of Southern California July 5 2018 Phase 1|Phase 2
NCT03012191 Completed Drug: Gentamicin Sulfate Recessive Dystrophic Epidermolysis Bullosa University of Southern California February 2 2017 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID