For research use only.
Catalog No.S4030 Synonyms: NSC-82261, SCH9724
Gentamycin Sulfate is a broad-spectrum, aminoglycoside antibiotic used for cell culture which inhibits protein synthesis in sensitive organisms.
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|Description||Gentamycin Sulfate is a broad-spectrum, aminoglycoside antibiotic used for cell culture which inhibits protein synthesis in sensitive organisms.|
Gentamycin Sulfate is a complex of three different closely related aminoglycoside sulfates, Gentamicins c1, c1a and c2, obtained from Micromonospora purpurea and related species.  They are broad-spectrum antibiotics, but may cause ear and kidney damage. Gentamycin Sulfate is reported to have activity against gram-negative bacteria, Pseudomonas aeruginosa, gram-positive Staphylococcus aureus and some mycoplasmas. Reported to have low toxicity to mammalian cells and viruses at certain concentrations, useful for long-term tissue culture studies with viruses.  Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. 
|In vitro||Water||100 mg/mL|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03845790||Recruiting||Other: Blood and microdialysis samples||Surgical Site Infection||Poitiers University Hospital||May 26 2019||Phase 1|
|NCT03632109||Terminated||Drug: gentamicin 360mg IM||Pharyngeal Gonococcal Infection||University of Washington|National Institute of Allergy and Infectious Diseases (NIAID)||September 17 2018||Phase 2|Phase 3|
|NCT03392909||Recruiting||Drug: Gentamicin||Recessive Dystrophic Epidermolysis Bullosa||University of Southern California||July 5 2018||Phase 1|Phase 2|
|NCT03012191||Completed||Drug: Gentamicin Sulfate||Recessive Dystrophic Epidermolysis Bullosa||University of Southern California||February 2 2017||Phase 1|Phase 2|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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