Home Stem Cells & Wnt Notch inhibitor FLI-06 For research use only.

FLI-06

FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM.

FLI-06 Chemical Structure

FLI-06 Chemical Structure

CAS No. 313967-18-9

Purity & Quality Control

Batch: S739901 DMSO]88 mg/mL]false]Ethanol]8 mg/mL]false]Water]Insoluble]false Purity: 99.9%
99.9

FLI-06 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-231 cells Cytotoxicity assay Cytotoxicity against human MDA-MB-231 cells, IC50=4 μM 26115571
Click to View More Cell Line Experimental Data

Biological Activity

Description FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM.
Targets
Notch [1]
(Cell-based assay)
2.3 μM(EC50)
In vitro
In vitro In HeLa NotchΔE-eGFP cells, FLI-06 blocks Notch trafficking and processing. FLI-06 changes the maturation pattern of APP and abolishes the shedding of APPs in HEK293 cells stably expressing a mutated APP that yields robust amounts of amyloid β. FLI-06 disrupt the Golgi apparatus, and inhibits general secretion at a step before exit from the endoplasmic reticulum (ER), which is accompanied by a tubule-to-sheet morphological transition of the ER. [1]
In Vivo
In vivo FLI-06 (50 μM) inhibits endogenous Notch signaling in zebrafish embryos. [1]
Animal Research Animal Models Zebrafish embryos.
Dosages 50 μM
Administration --

Chemical Information & Solubility

Molecular Weight 438.52 Formula

C25H30N2O5
CAS No. 313967-18-9 SDF Download FLI-06 SDF
Smiles CC1=C(C(C2=C(N1)CC(CC2=O)(C)C)C3=CC=C(C=C3)[N+](=O)[O-])C(=O)OC4CCCCC4
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 88 mg/mL ( (200.67 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 8 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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