Home Stem Cells & Wnt Notch inhibitor FLI-06

FLI-06

Catalog No.S7399

For research use only.

FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM.

FLI-06 Chemical Structure

CAS No. 313967-18-9

Selleck's FLI-06 has been cited by 12 Publications

2 Customer Reviews

Purity & Quality Control

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Biological Activity

Description FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM.
Targets
Notch [1]
(Cell-based assay)
2.3 μM(EC50)
In vitro

In HeLa NotchΔE-eGFP cells, FLI-06 blocks Notch trafficking and processing. FLI-06 changes the maturation pattern of APP and abolishes the shedding of APPs in HEK293 cells stably expressing a mutated APP that yields robust amounts of amyloid β. FLI-06 disrupt the Golgi apparatus, and inhibits general secretion at a step before exit from the endoplasmic reticulum (ER), which is accompanied by a tubule-to-sheet morphological transition of the ER. [1]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-231 cells MoDZR5l1d3SxeHnjbZR6KGG|c3H5 M{n4b2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOsJGlEPTB;NDFOwG0> MYKyOlEyPTV5MR?=
Click to View More Cell Line Experimental Data
In vivo FLI-06 (50 μM) inhibits endogenous Notch signaling in zebrafish embryos. [1]

Protocol (from reference)

Animal Research:

[1]
  • Animal Models: Zebrafish embryos.
  • Dosages: 50 μM
  • Administration: --

Solubility (25°C)

In vitro

 DMSO 88 mg/mL
(200.67 mM)
Ethanol 8 mg/mL
(18.24 mM)
Water Insoluble

Chemical Information

Molecular Weight 438.52
Formula

C25H30N2O5
CAS No. 313967-18-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C(C2=C(N1)CC(CC2=O)(C)C)C3=CC=C(C=C3)[N+](=O)[O-])C(=O)OC4CCCCC4

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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