For research use only.
CAS No. 313967-18-9
FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM.
Selleck's FLI-06 has been cited by 11 publications
2 Customer Reviews
In the presence of FLI-06 (100 nM), wound healing assay was conducted to evaluate the cell motility after transfection. Data were from three independent experiments and were Mean ± SD. values. **P < 0.01 compared to cells transfected with vector, ##P < 0.01 compared to cells transfected with ZFR. Scale bar represents 200 μm.
Am J Cancer Res, 2017, 7(9):1804-1819. FLI-06 purchased from Selleck.
G. In the presence of FLI-06, wound healing assay was conducted to evaluate the cell motility after transfection. Scale bar represents 200 μm. H. In the presence of FLI-06, invasion assay was conducted to evaluate the cell invasiveness after transfection. Data were from three independent experiments and were Mean ± SD. **p < 0.01 compared to cells transfected with vector, ##p < 0.01 compared to cells transfected with ACTG2. Scale bar represents 100 μm.
Biochem Biophys Res Commun, 2016. FLI-06 purchased from Selleck.
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Choose Selective Notch Inhibitors
|Description||FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM.|
In HeLa NotchΔE-eGFP cells, FLI-06 blocks Notch trafficking and processing. FLI-06 changes the maturation pattern of APP and abolishes the shedding of APPs in HEK293 cells stably expressing a mutated APP that yields robust amounts of amyloid β. FLI-06 disrupt the Golgi apparatus, and inhibits general secretion at a step before exit from the endoplasmic reticulum (ER), which is accompanied by a tubule-to-sheet morphological transition of the ER. 
|In vivo||FLI-06 (50 μM) inhibits endogenous Notch signaling in zebrafish embryos. |
|In vitro||DMSO||88 mg/mL (200.67 mM)|
|Ethanol||8 mg/mL (18.24 mM)|
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In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
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|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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