Bacitracin

Catalog No.S4146

For research use only.

Bacitracin is a mixture of related cyclic polypeptides produced by organisms of the licheniformis group of Bacillus subtilis var Tracy, which disrupts both gram positive and gram negative bacteria by interfering with cell wall and peptidoglycan synthesis.

Bacitracin Chemical Structure

CAS No. 1405-87-4

Selleck's Bacitracin has been cited by 3 Publications

Purity & Quality Control

Choose Selective Selection Antibiotics for Transfected Cell Inhibitors

Biological Activity

Description Bacitracin is a mixture of related cyclic polypeptides produced by organisms of the licheniformis group of Bacillus subtilis var Tracy, which disrupts both gram positive and gram negative bacteria by interfering with cell wall and peptidoglycan synthesis.
In vitro

Bacitracin is a potent antibiotic against gram-positive cocci, staphlococci, streptococci, corynebacteria, Treponema pallidum, T. vincenti, Actinomyces israeli, anaerobic cocci, clostridia, neisseria, most gonococci and meingococci, but it is relatively ineffective against most other gram-negative bacteria. Archaebacteria is also sensitive to Bacitracin. Of this group, Methanobacterium arbophilicum, M. M.O.H., M. O.H.G. and M. formicium showed inhibition by Bacitracin while Methanospirilum and Methanosarcina are insensitive to the antibiotic. Bacitracin affects a number of biochemical processes in bacteria, including metal ion transport, peptidoglycan synthesis, membrane permeability, and biosynthesis of inducible enzymes. Many of the biological properties of Bacitracin can be attributed to its high affinity for polyisoprenyl pyrophosphates. Aside from the antibiotic activity of the Bacitracins, these peptides have proven useful as a tool for investigating lipid-protein interactions at the molecular level. [1] Bacitracin is also a known inhibitor of insulin degradation by both isolated cells and subcellular organelles, which inhibits the ability of purified glutathione-insulin transhydrogenase to split insulin into its constituent A and B chains. At concentrations of 90 and 300μM, Bacitracin inhibits 50 and 90%, respectively, of the degrading activity of the purified enzyme. Similarly, degradation by crude liver lysates is inhibited 50 and 90% by 70 and 250 μM Bacitracin, respectively. Bacitracin decreases both the Vmax and affinity of the enzyme for insulin. [2] Bacitracin is able to inhibit the reductive function of the plasma membrane by against protein disulfide-isomerase. Bacitracin is able to induce inhibition of the cleavage of a disulfide-linked iodotyramine/poly(D-lysine) conjugate which bounds nonspecifically to the surface of CHO cells. Bacitracin also inhibits the cytotoxicity of diphtheria toxin, adisulfide-linked heterodimer that binds to a specific surface receptor and must undergo chain separation to exert its cytotoxicity. [3]

Protocol (from reference)

Solubility (25°C)

In vitro

Water 100 mg/mL
(70.98 mM)


Chemical Information

Molecular Weight 1408.67
Formula

C65H101N17O16S

CAS No. 1405-87-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(C)C1C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCCCCC(C(=O)NC(C(=O)N1)CCCN)NC(=O)C(C(C)CC)NC(=O)C(CCC(=O)O)NC(=O)C(CC(C)C)NC(=O)C2CSC(=N2)C(C(C)CC)N)CC(=O)N)CC(=O)O)CC3=CN=CN3)CC4=CC=CC=C4

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Bacitracin | Bacitracin ic50 | Bacitracin price | Bacitracin cost | Bacitracin solubility dmso | Bacitracin purchase | Bacitracin manufacturer | Bacitracin research buy | Bacitracin order | Bacitracin mouse | Bacitracin chemical structure | Bacitracin mw | Bacitracin molecular weight | Bacitracin datasheet | Bacitracin supplier | Bacitracin in vitro | Bacitracin cell line | Bacitracin concentration | Bacitracin nmr