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Azithromycin (CP-62993)

Licensed by Pfizer Catalog No.S1835 Synonyms: XZ-450

For research use only.

Azithromycin (CP-62993, XZ-450) is an antibiotic by inhibiting protein synthesis, used for the treatment of bacterial infections.

Azithromycin (CP-62993) Chemical Structure

CAS No. 83905-01-5

Selleck's Azithromycin (CP-62993) has been cited by 19 publications

Purity & Quality Control

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Biological Activity

Description Azithromycin (CP-62993, XZ-450) is an antibiotic by inhibiting protein synthesis, used for the treatment of bacterial infections.
Targets
protein synthesis [1]
In vitro

Azithromycin reduces about 40% of IL-8 mRNA and protein expression in cystic fibrosis (CF) cells reaching the levels of non-CF cells. Azithromycin results in 50% and 70% reduction of NF-kappaB and AP-1 DNA binding, respectively, leading to levels of non-CF cells. [1] Azithromycin significantly enhances the intensity of a co-stimulatory molecule, CD80, on DCs but not CD86 and CD40 in dendritic cells (DCs). Azithromycin significantly increases the production of IL-10 and Clarithromycin (CAM) significantly inhibits the production of IL-6 by DCs. Azithromycin increases IL-10 and CAM decreases IL-2 productions significantly, when naive T cells derived from spleen are co-cultured with DCs treated in advance with LPS and these macrolides. [2] Azithromycin selectively inhibits fluid-phase endocytosis of horseradish peroxidase and lucifer yellow in J774 mouse macrophages. Azithromycin delays sequestration of receptor-bound transferrin and peroxidase-anti-peroxidase immune complexes into cell-surface endocytic pits and vesicles. Azithromycin down-regulates cell surface transferrin receptors, but not Fc gamma receptors, by causing a major delay in the accessibility of internalized transferrin receptors to the recycling route, without slowing down subsequent efflux, resulting in redistribution of the surface pool to an intracellular pool. [3] Azithromycin inserts into the DOPC lipid bilayer, so as to decrease its cohesion and to facilitate the merging of DPPC into the DOPC fluid matrix. [4]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa 299 NWC0Z4hWSW62aXPocIFugWSrYXygZZN{[Xl? MkPwOFQhfG9iNEigbJJ{ NFT6V|ZCdnSrY3jsZY16\GmjbDDhZ5Rqfmm2eTDh[4FqdnO2IFPocIFugWSrYTD0doFkcG:vYYTpd{B{\XKxdnHyJGwzKGmwZnXjeIVlKGmwIHj1cYFvKEinTHGgNlk6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDueY1j\XJib3[gbY5kdHW|aX;uJIJw\GmnczDt[YF{fXKnZDDh[pRmeiB2NDD0c{A1QCCqcoOgZpkhTEGSSTDzeIFqdmmwZz3iZZNm\CCKQ2OgZZN{[XluIFnDOVA:OC55MkhOwG0v Mn;mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{M{K1PFQoRjJ7MkOyOVg1RC:jPh?=
THP1 M372OmZ2dmO2aX;uJIF{e2G7 MYmzNEBucW6| NFzzWWJFenWpIIXweIFs\SCrbjDoeY1idiCWSGCxJINmdGy|IHH0JJBJKDZidH:gO{Bi\nSncjCzNEBucW6| MmnSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3NkSzOlUoRjF7NU[0N|Y2RC:jPh?=
THP1 NUPlcYdbTnWwY4Tpc44h[XO|YYm= M1jDe|I1KGi{cx?= M3q5RWRzfWdidYD0ZYtmKGmwIHj1cYFvKFSKUEGgZ4VtdHNiYX\0[ZIhOjRiaILzJIlvKHC{ZYPlcoNmKG:oII\ldoFx[W2rbB?= NWjVeIVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1OlQ{PjVpPkG5OVY1OzZ3PD;hQi=>
THP1 MmHZSpVv[3Srb36gZZN{[Xl? Mkn1NlQhcHK| NGPyeZlFenWpIIXweIFs\SCrbjDoeY1idiCWSGCxJINmdGy|IHHmeIVzKDJ2IHjyd{BqdiCycnXz[Y5k\SCxZjDn[Y1ncWK{b4rpcC=> NH[5Z3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW2OFM3PSd-MUm1OlQ{PjV:L3G+
Hep2 NH3lco9CdnSrY3jsZY16\GmjbDDhd5NigQ>? NFKweok1KHWpL33s NV\2Oo9KQCCqcoO= NGHMfG9CdnSrLVPocIFugWSrYXygZYN1cX[rdImgZYdicW6|dDDDbIxidXmmaXGgeJJi[2ixbXH0bZMhW2W{b4\hdkBNT1ZvTEKgbY5n\WO2ZXSgbY4hUGWyMjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4he2m8ZTDhcoQhdnWvYnXyJI9nKGOqbHHtfYRq[WxiaX7jcJV{cW:wIHH0JFQhfWdxbXygeJJm[XSnZDDheEA5KGi{czDwc5N1NWmwZnXjeIlwdiCjbnSgNlQhcHK|IHzheIVzKHKnLXnu[oVkfGmwZzDmdoV{cCCKZYCyJINmdGy|IH3vco9t[Xl? M1vYUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzN{MkK3PVQ5Lz5|MkKyO|k1QDxxYU6=
Vero E6 NV\EWo8xSW62aY\pdoFtKGG|c3H5 M2DOTFIh\GG7cx?= MULBcpRqfmm{YXyg[YZncWOjY4mgZYdicW6|dDDTRXJUNUOxVj2yJEh{fHKjaX6gRoF3WGG2MTmgbY4hXmW{bzDFOkBk\WyuczDhd5Nme3OnZDDifUBqdmirYnn0bY9vKG:oII\pdoFtKFKQQTDy[ZBtcWOjdHnvckBu\WG|dYLl[EBjgSCUVD3QR3Ih[W[2ZYKgNkBl[Xm|LDDFR|UxRTJwMUNOwG0v M3;a[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzQS9pPlPoSW1DVDxxYU6=
Vero E6 NWS4[IV1SW62aY\pdoFtKGG|c3H5 MUOyJIRigXN? NGfzWZNCdnSrdnnyZYwh\W[oaXPhZ5kh[WejaX7zeEBUSVKVLVPvWk0zKCi|dILhbY4hSmG4UHH0NUkhcW5iVnXyc{BGPiClZXzsd{Bie3Onc4Pl[EBjgSCrbnjpZol1cW:wIH;mJJZqemGuIGLORUBz\XCuaXPheIlwdiCvZXHzeZJm\CCkeTDSWE1RS1JiYX\0[ZIhOiCmYYnzMEBGSzlyPUiuOlXPxE1w M4LRRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUzQS9pPlPoSW1DVDxxYU6=
Click to View More Cell Line Experimental Data

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 748.98
Formula

C38H72N2O12
CAS No. 83905-01-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC1C(C(C(N(CC(CC(C(C(C(C(C(=O)O1)C)OC2CC(C(C(O2)C)O)(C)OC)C)OC3C(C(CC(O3)C)N(C)C)O)(C)O)C)C)C)O)(C)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

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%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05398055 Recruiting Drug: Azithromycin + Doxycycline|Drug: Placebo + Doxycycline Rocky Mountain Spotted Fever Hospital General de Mexicali May 12 2022 Phase 3
NCT05465304 Completed Drug: Azithromycin Pregnant With Complication Beni-Suef University October 1 2021 --
NCT04326478 Recruiting Drug: Azithromycin|Biological: Placebo Cholera Massachusetts General Hospital|International Centre for Diarrhoeal Disease Research Bangladesh October 31 2021 Phase 2
NCT04669288 Recruiting Drug: Azithromycin|Drug: Placebo Asthma|Wheezing University of Arizona|University of Utah|Emory University|Morgan Stanley Children''s Hospital|University of Pittsburgh|Children''s Hospital and Health System Foundation Wisconsin|Children''s Hospital of Philadelphia|Children''s Hospital Medical Center Cincinnati|Boston Children''s Hospital September 22 2021 Phase 3
NCT04617626 Completed Drug: Azithromycin Child Mortality|Azithromycin FHI 360|National Program for the Fight Against Neglected Tropical Diseases Côte d''Ivoire November 20 2020 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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