Azithromycin

Name: Azithromycin
Brand: Selleck Chemicals
SKU: S1835
Rating: 5 (10 reviews)

For research use only.

Licensed by Pfizer Catalog No.S1835 Synonyms: CP-62993, XZ-450

10 publications

CAS No. 83905-01-5

Azithromycin (CP-62993, XZ-450) is an antibiotic by inhibiting protein synthesis, used for the treatment of bacterial infections.

Selleck's Azithromycin has been cited by 10 publications

Biomolecules, 2020, 10(10)E1395

PubMed: 33007949 ( click the link to review the publication )

Cancer Cell Int, 2020, 19;20:58

PubMed: 32099531 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00420-z

PubMed: 32870449 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00425-8

PubMed: 32886306 ( click the link to review the publication )

bioRxiv, 2020, 2020.10.02.324228

PubMed: 33024968 ( click the link to review the publication )

Adv Ther (Weinh), 2020, 2000034

PubMed: 32838027 ( click the link to review the publication )

Clin Transl Med, 2020, 9(1):22

PubMed: 32112175 ( click the link to review the publication )

mBio, 2018, 9(5)

PubMed: 30377287 ( click the link to review the publication )

J Med Chem, 2018, 61(9):4189-4202

PubMed: 29608068 ( click the link to review the publication )

Proc Natl Acad Sci U S A, 2016, 113(50):14408-14413

PubMed: 27911847 ( click the link to review the publication )

Purity & Quality Control

Choose Selective Antibiotics for Mammalian Cell Culture Inhibitors

Biological Activity

Description

Azithromycin (CP-62993, XZ-450) is an antibiotic by inhibiting protein synthesis, used for the treatment of bacterial infections.

Targets

protein synthesis ^[1]

In vitro

Azithromycin reduces about 40% of IL-8 mRNA and protein expression in cystic fibrosis (CF) cells reaching the levels of non-CF cells. Azithromycin results in 50% and 70% reduction of NF-kappaB and AP-1 DNA binding, respectively, leading to levels of non-CF cells. ^[1] Azithromycin significantly enhances the intensity of a co-stimulatory molecule, CD80, on DCs but not CD86 and CD40 in dendritic cells (DCs). Azithromycin significantly increases the production of IL-10 and Clarithromycin (CAM) significantly inhibits the production of IL-6 by DCs. Azithromycin increases IL-10 and CAM decreases IL-2 productions significantly, when naive T cells derived from spleen are co-cultured with DCs treated in advance with LPS and these macrolides. ^[2] Azithromycin selectively inhibits fluid-phase endocytosis of horseradish peroxidase and lucifer yellow in J774 mouse macrophages. Azithromycin delays sequestration of receptor-bound transferrin and peroxidase-anti-peroxidase immune complexes into cell-surface endocytic pits and vesicles. Azithromycin down-regulates cell surface transferrin receptors, but not Fc gamma receptors, by causing a major delay in the accessibility of internalized transferrin receptors to the recycling route, without slowing down subsequent efflux, resulting in redistribution of the surface pool to an intracellular pool. ^[3] Azithromycin inserts into the DOPC lipid bilayer, so as to decrease its cohesion and to facilitate the merging of DPPC into the DOPC fluid matrix. ^[4]

Protocol

References

[4] Fa N, et al. Biochim Biophys Acta, 2007, 1768(7), 1830-1838.

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Solubility (25°C)

In vitro	DMSO	100 mg/mL (133.51 mM)
	Ethanol	100 mg/mL (133.51 mM)
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Azithromycin SDF

Molecular Weight	748.98
Formula	C₃₈H₇₂N₂O₁₂
CAS No.	83905-01-5
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	CP-62993, XZ-450
Smiles	CCC1C(C(C(N(CC(CC(C(C(C(C(C(=O)O1)C)OC2CC(C(C(O2)C)O)(C)OC)C)OC3C(C(CC(O3)C)N(C)C)O)(C)O)C)C)C)O)(C)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

g

Dosing volume per animal

ul

Number of animals

Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2020-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04669288	Not yet recruiting	Drug: Azithromycin\|Drug: Placebo	Asthma\|Wheezing	University of Arizona\|University of Utah\|Emory University\|Morgan Stanley Children''s Hospital\|University of Pittsburgh\|Children''s Hospital and Health System Foundation Wisconsin\|Children''s Hospital of Philadelphia\|Children''s Hospital Medical Center Cincinnati\|Boston Children''s Hospital	May 2021	Phase 3
NCT04617626	Not yet recruiting	Drug: Azithromycin	Child Mortality\|Azithromycin	FHI 360\|Côte d''Ivoire National Program for the Fight Against Neglected Tropical Diseases	November 15 2020	Not Applicable
NCT04424511	Recruiting	Drug: Placebo\|Drug: Azithromycin	Mortality	Tampere University\|Center for Vaccine Development CVD-Mali Bamako Mali\|University College London UK.\|Tro Da Ltd UK\|Duke-NUS Graduate Medical School\|Bill and Melinda Gates Foundation (Funder)\|Pfizer Inc. (Provider of study drugs)	October 15 2020	Phase 3
NCT04294069	Recruiting	Drug: Azithromycin 500 mg\|Drug: Azithromycin Oral Product	Preterm Premature Rupture of Membrane	Thomas Jefferson University	September 14 2020	Phase 4

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Azithromycin