Quizartinib (AC220)

Catalog No.S1526

For research use only.

Quizartinib (AC220) is a second-generation FLT3 inhibitor for Flt3(ITD/WT) with IC50 of 1.1 nM/4.2 nM in MV4-11 and RS4;11 cells, respectively, 10-fold more selective for Flt3 than KIT, PDGFRα, PDGFRβ, RET, and CSF-1R. Quizartinib (AC220) induces apoptosis of tumor cells. Phase 3.

Quizartinib (AC220) Chemical Structure

CAS No. 950769-58-1

Selleck's Quizartinib (AC220) has been cited by 114 publications

Purity & Quality Control

Choose Selective Target Protein Ligand Inhibitors

Biological Activity

Description Quizartinib (AC220) is a second-generation FLT3 inhibitor for Flt3(ITD/WT) with IC50 of 1.1 nM/4.2 nM in MV4-11 and RS4;11 cells, respectively, 10-fold more selective for Flt3 than KIT, PDGFRα, PDGFRβ, RET, and CSF-1R. Quizartinib (AC220) induces apoptosis of tumor cells. Phase 3.
Features The most potent cellular FLT3-ITD inhibitor.
Targets
FLT3 (ITD) [1]
(MV4-11 cells)
FLT3 (WT) [1]
(RS4;11 cells)
1.1 nM 4.2 nM
In vitro

AC220, a unique, potent and selective inhibitor of FLT3, has high affinity for FLT3 with a Kd value of 1.6 nM. AC220 inhibits the autophosphorylation of FLT3 in the human leukemia cell lines MV4-11 which harbor a homozygous FLT3-ITD mutation and is FLT3 dependent, and RS4;11 which expresses wild-type FLT3 with IC50 values of 1.1 nM and 4.2 nM, respectively. AC220 is the most potent cellular FLT3-ITD inhibitor, leading to the most significant inhibition of MV4-11 cell proliferation with IC50 of 0.56 nM compared to all other FLT3 inhibitors whose IC50 values range from 0.87 nM to 64 nM. AC220 has no inhibitory activity against the proliferation of A375 cells which harbor an activating mutation in BRAF and are not FLT3 dependent, indicating a large window between FLT3 inhibition and general cytotoxic effects. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HL60/VCR M4m5TGZ2dmO2aX;uJGF{e2G7 NHXSTIgxNjFvMUCg{txO NH3oUms{OCCvaX6= M3XJ[oVvcGGwY3XzJJVxfGGtZTDv[kB{fWK|dILheIV{KG:oIFHCR2czKGGwZDDBRmNDOSCrbjDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IH3hco5meg>? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4N{G3O{c,OjN7NkexO|c9N2F-
K562/ABCB1 NUTnUVNETnWwY4Tpc44hSXO|YYm= NGnFUowxNjFvMUCg{txO MUezNEBucW5? NXvTXHBG\W6qYX7j[ZMhfXC2YXvlJI9nKHO3YoP0doF1\XNib3[gRWJETzJiYX7kJGFDS0JzIHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{ MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4N{G3O{c,OjN7NkexO|c9N2F-
8226/MR20  NXewWJZzTnWwY4Tpc44hSXO|YYm= NEXnRmYxNjFvMUCg{txO NUn1XpBKOzBibXnu MWflcohidmOnczD1dJRic2Vib3[gd5Vje3S{YYTld{Bw\iCDQlPHNkBidmRiQVLDRlEhcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4N{G3O{c,OjN7NkexO|c9N2F-
K562/ABCG2 M{S1O2Z2dmO2aX;uJGF{e2G7 NI\uUZIxNjFvMUCg{txO NWHvVoo2OzBibXnu MlPG[Y5p[W6lZYOgeZB1[WunIH;mJJN2[nO2cnH0[ZMhd2ZiQVLDS|Ih[W6mIFHCR2IyKGmwIHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRibXHucoVz NFS0fmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2O|E4Pyd-MkO5OlcyPzd:L3G+
MCF-7 FLV1000 MVLLbY5ie2ViQYPzZZk> MlTRNQKBmzNyINM1US=> M4fTbFUhdWmw M3\RVoRm[3KnYYPld{BcOTJ3SW2tTWFCWCCyaH;0c4xi[mWuaX7nJI9nKEGEQ1KxJIF1KEmFNUCgc4YhOy5|IN88US=> MmHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkexO|coRjJ|OU[3NVc4RC:jPh?=
MCF-7 FLV1000 NYnJfnFtU2mwYYPlJGF{e2G7 NHH0SFIx6oDVM{CgxtVO MonIOUBucW5? MoHF[IVkemWjc3XzJHsyOjWLXT3JRWFRKHCqb4TvcIFj\Wyrbnegc4YhSUKFQkKgZZQhUUN3MDDv[kAxNjB5IN88US=> M3PYbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OU[3NVc4Lz5{M{m2O|E4PzxxYU6=
K562/ABCG2 M3PZfGNmdGxiVnnhZoltcXS7IFHzd4F6ew>? MVOwMlEwOC53L{GgxtVO M2TtVlk3KGh? NFj1[GJ{\W6|aYTpfoV{KEt3NkKvRWJETzJiY3XscJMhfG9ibXn0c5hidnS{b37lJJRweG:2ZXPhcuKh MnvYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkexO|coRjJ|OU[3NVc4RC:jPh?=
8226/MR20 M2POdGNmdGxiVnnhZoltcXS7IFHzd4F6ew>? MlniNE4yKML3TR?= MmP5PVYhcA>? NVrRZWhVe2Wwc3n0bZpmeyCNNU[yM2FDS0d{IHPlcIx{KHSxIH3peI95[W62cn;u[UB1d3CxdHXjZY7DqA>? M1WxNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OU[3NVc4Lz5{M{m2O|E4PzxxYU6=
HMC1.1 NYPz[mZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHo[JBKSzVyPUG0JI5O Ml\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
HMC1.2 NV\qemRoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDsfo9KSzVyPUG3Nlchdk1? MnrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
p815 MnjTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;jTWM2OD12NEWgcm0> NVzEeoV2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
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M-07e + SCF NETKZ25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTd5IH7N MkLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
EOL-1 NV7LS4V2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPWNpBFUUN3ME2xJI5O M{LN[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
MV4;11 NY\5XoEyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRCBzIH7N NXTE[JBGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
MOLM14 MkTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWiyZWtZUUN3MEygNUBvVQ>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
Pat.221 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4Tn[2lEPTB;Nke1JI5O NH7rdYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.279 Mlj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTN2M{Sgcm0> NH;IfJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.299 NWLWVIl2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DFPGlEPTB;N{K0PEBvVQ>? MojFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
Pat.305 NX7HOFR6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3f2e2lEPTB;N{C3PUBvVQ>? M{L1blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
Pat.375 Mn\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\OTWM2OD13MEOgcm0> NFzGN3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.379 NEHGO|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLQTWM2OD16ME[gcm0> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
Pat.368 M1L6Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTJ5MECgcm0> NGXVW3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.601 MmfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fQ[mlEPTB;MUG1N{BvVQ>? NGXEN3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
HMC1.1 NUTmOGN5SXCxcITvd4l{KEG|c3H5 NX7pRoJuUUN3ME2zNUBvVQ>? Ml;oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
p815 NXuxTVd3SXCxcITvd4l{KEG|c3H5 MWPJR|UxRTN2MTDuUS=> NX;FcHg2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
Kasumi-1 M{ixRWFxd3C2b4Ppd{BCe3OjeR?= NXzUe4ZEUUN3ME22O{BvVQ>? M3vaPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
M-07e + SCF NVvwdmZmSXCxcITvd4l{KEG|c3H5 MmPzTWM2OD15ODDuUS=> MnXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
EOL-1 NUDE[nBSSXCxcITvd4l{KEG|c3H5 NYrPT|dTUUN3MEygNUBvVQ>? NYnS[JpmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
MV4;11 M{HMdWFxd3C2b4Ppd{BCe3OjeR?= MV7JR|UxRTJibl2= MmDCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
MOLM14 MXzBdI9xfG:|aYOgRZN{[Xl? MoK1TWM2OD1|IH7N NHjMcnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
GIST822 NGTwb3VCeG:ydH;zbZMhSXO|YYm= NHTnd3dKSzVyPUGwPUBvVQ>? NGfhSJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.368 MoLBRZBweHSxc3nzJGF{e2G7 NEXYc|RKSzVyPUK5PVghdk1? NILaeXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.601 NXG1OG1kSXCxcITvd4l{KEG|c3H5 NGLaSlRKSzVyPUi3OkBvVQ>? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
MV4-11 NUPoXm15T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml25O|IhcA>? MoXtTWM2OD1yLkOgcm0> NFXCXnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxNlk{OSd-MkO0NVI6OzF:L3G+
MOLM-14 NHWxb3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXl[YU4OiCq MVvJR|UxRTBwMTDuUS=> NU\x[ndnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVI6OzFpPkKzOFEzQTNzPD;hQi=>
SEM-K2 Mn;YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV23NkBp MVrJR|UxRTBwNDDuUS=> NUHHXlY5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVI6OzFpPkKzOFEzQTNzPD;hQi=>
RS4;11 NV:zZZpyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFf1coU4OiCq MoG5TWM2OD5zMDywNFAhdk1? NXrFUItuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVI6OzFpPkKzOFEzQTNzPD;hQi=>
THP-1 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVq3NkBp NXjXfpFQUUN3ME6xNEwxODBibl2= M{H2e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEGyPVMyLz5{M{SxNlk{OTxxYU6=
MV4-11 MVPBdI9xfG:|aYOgRZN{[Xl? MW[4M|I1KGh? M4PEXYlv\HWlZYOgd4lodmmoaXPhcpQh[W6mIHTvd4Uu\GWyZX7k[Y51KFCDUmCgZ4xm[X[jZ3WgZY5lKGGlY4XteYxifGmxbjDv[kB{fWJvMl6gSG5C MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRzMkmzNUc,OjN2MUK5N|E9N2F-
MOLM-14 NVLHcHE{SXCxcITvd4l{KEG|c3H5 MVK4M|I1KGh? NVjuZWZvcW6mdXPld{B{cWewaX\pZ4FvfCCjbnSg[I9{\S2mZYDlcoRmdnRiUFHSVEBkdGWjdnHn[UBidmRiYXPjeY12dGG2aX;uJI9nKHO3Yj2yUkBFVkF? M2DvRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEGyPVMyLz5{M{SxNlk{OTxxYU6=
SEM-K2 NFu4ZVhCeG:ydH;zbZMhSXO|YYm= MVS4M|I1KGh? NUHQN2dKcW6mdXPld{B{cWewaX\pZ4FvfCCjbnSg[I9{\S2mZYDlcoRmdnRiUFHSVEBkdGWjdnHn[UBidmRiYXPjeY12dGG2aX;uJI9nKHO3Yj2yUkBFVkF? MnH0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MUK5N|EoRjJ|NEGyPVMyRC:jPh?=
AML Ml\yR5l1d3SxeHnjbZR6KGG|c3H5 Mm\ZR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRW1NKGOnbHzzJIl{d2yjdHXkJIZzd21icnXsZZB{\WRiYXP1eIUhdXmnbH;p[EBt\XWtZX3pZUBx[XSrZX70JIhiemKxcnnu[{BHVFR|IFnUSEBufXSjdHnvckBjgSCPVGSgZZN{[XluIFnDOVAhRSByLkCwNFMh|ryPLh?= NVu0fWtTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm2OVQ1ODhpPkG5OlU1PDB6PD;hQi=>
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MV4-11 NEPFVpNHfW6ldHnvckBie3OjeR?= NHXldIEyOCCvZz;r[y=> M{DIcFI1KGi{cx?= NYCzNYhmUW5idnn2c{BqdmirYnn0bY9vKG:oIF\MWFMhUVSGIH31eIFvfCCrbjDoeY1idiCPVkStNVEh[2WubIOgfIVvd2e{YX\0[YQhcW5iYYTofY1q[yCwdT;ueUBud3W|ZTDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCVVFHUOUBxcG:|cHjvdplt[XSrb36gZZQhYTZ7NDDheEAyOCCvZz;r[{whcXBiYX\0[ZIhOjRiaILzJIJ6KGmvbYXuc4Jtd3RibXX0bI9lKHKnbHH0bZZmKHSxIHPvcpRzd2x? NWTwcYs1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2O|IxPDlpPkK5OlczODR7PD;hQi=>
MV4-11 NI\Hd4tHfW6ldHnvckBie3OjeR?= MWiwMlEhfU1? Ml[xOEBpenN? NYLO[GJtUW6qaXLpeIlwdiCxZjDGUHQ{KGmwIHj1cYFvKE2YND2xNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iQVvUJJBpd3OyaH;yfYxifGmxbjDheEBU\XJ2N{OgdoV{cWS3ZTDheEAxNjFidV2gZYZ1\XJiNDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz M1LUVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEm0PVQ1Lz5{OUi5OFk1PDxxYU6=
MV4-11 NVuzSmJzTnWwY4Tpc44h[XO|YYm= Mm\uNE4yKHWP NGHLb2M1KGi{cx?= M1HF[GlvcGmkaYTpc44hd2ZiRlzUN{BqdiCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFOWQWS1JJBpd3OyaH;yfYxifGmxbjDheEBVgXJ4OUSgdoV{cWS3ZTDheEAxNjFidV2gZYZ1\XJiNDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MkLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6OUS5OFQoRjJ7OEm0PVQ1RC:jPh?=
MV4-11 NFz0O|ZHfW6ldHnvckBie3OjeR?= NHjvUGkxNjFidV2= NXy0WYVYPCCqcoO= MULJcohq[mm2aX;uJI9nKE[OVEOgbY4hcHWvYX6gUXY1NTFzIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBGWktzL{KgdIhwe3Cqb4L5cIF1cW:wIHH0JHRpejJyMj;UfZIzODRicnXzbYR2\XNiYYSgNE4yKHWPIHHmeIVzKDRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh7NEm0OEc,Ojl6OUS5OFQ9N2F-
MV4-11 NGfGS2ZHfW6ldHnvckBie3OjeR?= NYrTVm1LOC5zIIXN NF72TFg1KGi{cx?= NIS3U4tKdmirYnn0bY9vKG:oIF\MWFMheGixc4Doc5J6dGG2aX;uJIF1KFS7ckW4PU82QTFicnXzbYR2\XNiaX6gbJVu[W5iTW[0MVEyKGOnbHzzJIF1KDBwMTD1UUBi\nSncjC0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NY\i[|dvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm4PVQ6PDRpPkK5PFk1QTR2PD;hQi=>
Assay
Methods Test Index PMID
Western blot p-STAT5 / STAT5 / β-catenin / p-AKT / AKT / p-ERK / ERK / p-S6 / S6 ; phospho-FLT3 / FLT3 28625976 22875611
Immunofluorescence WGA / FLT3 28895560
Growth inhibition assay Cell viability 23967177
In vivo Oral administration of AC220 (10 mg/kg) induces time-dependent inhibition of FLT3 autophosphorylation in the FLT3-ITD–dependent MV4-11 tumor xenograft mouse model; the inhibition being 90% at 2 hours and 40% at 24 hours. AC220 significantly extends survival in a mouse model of FLT3-ITD AML with doses as low as 1 mg/kg given orally once a day. Treatment with AC220 at 10 mg/kg for 28 days results in rapid and complete regression of tumors in all mice with no tumor regrowth during the 60-day posttreatment period. AC220 displays more significant efficacy compared to sunitinib treatment which causes tumors to shrink slowly and resume growth immediately upon discontinuation of treatment in all but one of the mice. [1]

Protocol (from reference)

Kinase Assay:[1]
  • Inhibition of FLT3 autophosphorylation:

    To measure inhibition of FLT3 autophosphorylation, MV4-11 or RS4;11 cells are cultured in low serum media (0.5% FBS) overnight and seeded at a density of 400 000 cells per well in a 96-well plate the following day. The cells are incubated with different concentrations of AC220 for 2 hours at 37 °C. To induce FLT3 autophosphorylation in RS4;11 cells, 100 ng/mL FLT3 ligand is added for 15 minutes after the 2-hour AC220 incubation. Cell lysates are prepared and incubated in 96-well plates precoated with a total FLT3 capture antibody. The coated plates are incubated with either a biotinylated antibody against FLT3 to detect total FLT3 or an antibody against phosphotyrosines to detect FLT3 autophosphorylation. In both cases, a SULFO-tagged streptavidin secondary antibody is used for electrochemiluminescence detection on the Meso Scale Discovery platform. The concentration of AC220 that inhibits FLT3-ITD or TLT3-WT autophosphorylation by 50% represents IC50 value

Cell Research:[1]
  • Cell lines: MV4-11 and RS4;11 cells
  • Concentrations: Dissolved in DMSO, final concentration ~20 μM
  • Incubation Time: 72 hours
  • Method: Cells are cultured overnight in low serum media (0.5% FBS), seeded in a 96-well plate at 40 000 cells per well and exposed to AC220 for 72 hours at 37 °C. Cell viability is measured using the Cell Titer-Blue Cell Viability Assa
Animal Research:[1]
  • Animal Models: Female NU/NU or severe combined immunodeficient mice implanted with MV4-11 cells
  • Dosages: ~10 mg/kg
  • Administration: Oral gavage

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
15% Captisol
For best results, use promptly after mixing.

30 mg/mL

Chemical Information

Molecular Weight 560.67
Formula

C29H32N6O4S

CAS No. 950769-58-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)(C)C1=CC(=NO1)NC(=O)NC2=CC=C(C=C2)C3=CN4C5=C(C=C(C=C5)OCCN6CCOCC6)SC4=N3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04459598 Completed Drug: Efavirenz|Drug: Quizartinib Healthy Subjects|Drug-drug Interaction|Pharmacokinetics|Quizartinib Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. September 8 2020 Early Phase 1
NCT04473664 Completed Drug: Quizartinib Hepatic Impairment|Moderate Impaired Hepatic Function Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. September 29 2020 Phase 1
NCT04459585 Completed Drug: Dabigatran Etexilate Mesylate|Drug: Quizartinib Healthy Subjects|Drug-drug Interaction|Pharmacokinetics|Quizartinib Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. August 28 2020 Early Phase 1
NCT04209725 Terminated Drug: CPX-351|Drug: Quizartinib Leukemia Myeloid Acute SCRI Development Innovations LLC June 3 2020 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Is it possible to alter the captisol concentration to make it more dissolvable i.e 20% or 25% captisol ?

Answer:
In 15% Captisol, the compound forms s suspension at 30mg/ml. Increasing the percentage of Captisol will not convert the mixture into solution. You can use suspension for oral gavage feeding.

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