Quizartinib (AC220)

Catalog No.S1526

For research use only.

Quizartinib (AC220) is a second-generation FLT3 inhibitor for Flt3(ITD/WT) with IC50 of 1.1 nM/4.2 nM in MV4-11 and RS4;11 cells, respectively, 10-fold more selective for Flt3 than KIT, PDGFRα, PDGFRβ, RET, and CSF-1R. Quizartinib (AC220) induces apoptosis of tumor cells. Phase 3.

Quizartinib (AC220) Chemical Structure

CAS No. 950769-58-1

Selleck's Quizartinib (AC220) has been cited by 105 publications

Purity & Quality Control

Choose Selective Target Protein Ligand Inhibitors

Biological Activity

Description Quizartinib (AC220) is a second-generation FLT3 inhibitor for Flt3(ITD/WT) with IC50 of 1.1 nM/4.2 nM in MV4-11 and RS4;11 cells, respectively, 10-fold more selective for Flt3 than KIT, PDGFRα, PDGFRβ, RET, and CSF-1R. Quizartinib (AC220) induces apoptosis of tumor cells. Phase 3.
Features The most potent cellular FLT3-ITD inhibitor.
Targets
FLT3 (ITD) [1]
(MV4-11 cells)
FLT3 (WT) [1]
(RS4;11 cells)
1.1 nM 4.2 nM
In vitro

AC220, a unique, potent and selective inhibitor of FLT3, has high affinity for FLT3 with a Kd value of 1.6 nM. AC220 inhibits the autophosphorylation of FLT3 in the human leukemia cell lines MV4-11 which harbor a homozygous FLT3-ITD mutation and is FLT3 dependent, and RS4;11 which expresses wild-type FLT3 with IC50 values of 1.1 nM and 4.2 nM, respectively. AC220 is the most potent cellular FLT3-ITD inhibitor, leading to the most significant inhibition of MV4-11 cell proliferation with IC50 of 0.56 nM compared to all other FLT3 inhibitors whose IC50 values range from 0.87 nM to 64 nM. AC220 has no inhibitory activity against the proliferation of A375 cells which harbor an activating mutation in BRAF and are not FLT3 dependent, indicating a large window between FLT3 inhibition and general cytotoxic effects. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HL60/VCR NYPu[2J{TnWwY4Tpc44hSXO|YYm= NVLkXpluOC5zLUGwJO69VQ>? M1rqbVMxKG2rbh?= M2rIbYVvcGGwY3XzJJVxfGGtZTDv[kB{fWK|dILheIV{KG:oIFHCR2czKGGwZDDBRmNDOSCrbjDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IH3hco5meg>? NGryTpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2O|E4Pyd-MkO5OlcyPzd:L3G+
K562/ABCB1 MnyySpVv[3Srb36gRZN{[Xl? NGDWR5UxNjFvMUCg{txO MUizNEBucW5? MXzlcohidmOnczD1dJRic2Vib3[gd5Vje3S{YYTld{Bw\iCDQlPHNkBidmRiQVLDRlEhcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI> MkDkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkexO|coRjJ|OU[3NVc4RC:jPh?=
8226/MR20  NX;GUmxjTnWwY4Tpc44hSXO|YYm= Mn3KNE4yNTFyIN88US=> NXHQTnlKOzBibXnu M3XYdoVvcGGwY3XzJJVxfGGtZTDv[kB{fWK|dILheIV{KG:oIFHCR2czKGGwZDDBRmNDOSCrbjDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IH3hco5meg>? NWrDTnYyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OlcyPzdpPkKzPVY4OTd5PD;hQi=>
K562/ABCG2 MYfGeY5kfGmxbjDBd5NigQ>? MVuwMlEuOTBizszN NYLhZnBlOzBibXnu NUnOO3Jm\W6qYX7j[ZMhfXC2YXvlJI9nKHO3YoP0doF1\XNib3[gRWJETzJiYX7kJGFDS0JzIHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{ NG\TPWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2O|E4Pyd-MkO5OlcyPzd:L3G+
MCF-7 FLV1000 M2rMSmtqdmG|ZTDBd5NigQ>? NEHBOIQx6oDVM{CgxtVO NUfB[ZBRPSCvaX6= MnPU[IVkemWjc3XzJHsyOjWLXT3JRWFRKHCqb4TvcIFj\Wyrbnegc4YhSUKFQkGgZZQhUUN3MDDv[kA{NjNizszN MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4N{G3O{c,OjN7NkexO|c9N2F-
MCF-7 FLV1000 NFHxXJZMcW6jc3WgRZN{[Xl? NFr5VpMx6oDVM{CgxtVO NHLSdmY2KG2rbh?= M{PLT4Rm[3KnYYPld{BcOTJ3SW2tTWFCWCCyaH;0c4xi[mWuaX7nJI9nKEGEQ1KyJIF1KEmFNUCgc4YhOC5yNzFOwG0> MoPVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkexO|coRjJ|OU[3NVc4RC:jPh?=
K562/ABCG2 NGLpZ4dE\WyuIG\pZYJqdGm2eTDBd5NigXN? MkXkNE4yNzBwNT:xJOK2VQ>? M2rYPFk3KGh? NUXK[oVke2Wwc3n0bZpmeyCNNU[yM2FDS0d{IHPlcIx{KHSxIH3peI95[W62cn;u[UB1d3CxdHXjZY7DqA>? M1iwXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OU[3NVc4Lz5{M{m2O|E4PzxxYU6=
8226/MR20 MYLD[YxtKF[rYXLpcIl1gSCDc4PhfZM> MYWwMlEhyrWP M1XmT|k3KGh? MnjTd4Vve2m2aYrld{BMPTZ{L1HCR2czKGOnbHzzJJRwKG2rdH;4ZY51em:wZTD0c5BwfGWlYX9CpC=> MkP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkexO|coRjJ|OU[3NVc4RC:jPh?=
HMC1.1 NU\PbZYzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHXU2NKSzVyPUG0JI5O MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
HMC1.2 NYWxZXZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LSXGlEPTB;MUeyO{BvVQ>? NWLz[4lKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
p815 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrnTWM2OD12NEWgcm0> NG\NblA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Kasumi-1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPqc3VKSzVyPUO2JI5O Mo\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
M-07e + SCF MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojpTWM2OD15NzDuUS=> NWm1[3RIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
EOL-1 NFnWfI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHkTWM2OD1zIH7N MkXiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
MV4;11 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vPVGlEPTB:IEGgcm0> NU\MN4I1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
MOLM14 NI\WWmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXVTXB{UUN3MEygNUBvVQ>? M2rIZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
Pat.221 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTZ5NTDuUS=> NH;6fnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.279 MnThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTN2M{Sgcm0> M36w[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
Pat.299 NV;zUXRWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\pbXgyUUN3ME23NlQ5KG6P MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
Pat.305 NFSwOYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTVTWM2OD15MEe5JI5O MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
Pat.375 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vUd2lEPTB;NUCzJI5O MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
Pat.379 M3rQOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnIWWVyUUN3ME24NFYhdk1? MmLBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
Pat.368 Mn7PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIWxUmRKSzVyPUK3NFAhdk1? M3y2R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
Pat.601 NYjSSVM2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTFzNUOgcm0> MlmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
HMC1.1 MUfBdI9xfG:|aYOgRZN{[Xl? NUPNUnJjUUN3ME2zNUBvVQ>? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
p815 MV\BdI9xfG:|aYOgRZN{[Xl? MoW5TWM2OD1|NEGgcm0> NWnNUIExRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
Kasumi-1 NXzSb4JHSXCxcITvd4l{KEG|c3H5 M1H5UmlEPTB;Nkegcm0> MmXmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
M-07e + SCF NVflSm5FSXCxcITvd4l{KEG|c3H5 MXjJR|UxRTd6IH7N NFPlRWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
EOL-1 NVfDXIprSXCxcITvd4l{KEG|c3H5 MVXJR|UxRCBzIH7N M{nMfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
MV4;11 NETEWnJCeG:ydH;zbZMhSXO|YYm= M2D6N2lEPTB;MjDuUS=> NW\ObHdIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
MOLM14 MXzBdI9xfG:|aYOgRZN{[Xl? M3THSWlEPTB;MzDuUS=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
GIST822 MljxRZBweHSxc3nzJGF{e2G7 MVrJR|UxRTFyOTDuUS=> NVS4Zos3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
Pat.368 M13LWWFxd3C2b4Ppd{BCe3OjeR?= NEDPOXNKSzVyPUK5PVghdk1? M4LMdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
Pat.601 MkD1RZBweHSxc3nzJGF{e2G7 NUHxWZBMUUN3ME24O|Yhdk1? NFzRUIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
MV4-11 M{WwZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TMbFczKGh? MkfITWM2OD1yLkOgcm0> NXHJUZhORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVI6OzFpPkKzOFEzQTNzPD;hQi=>
MOLM-14 NWG0NFNPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXO3NkBp NVXhdnVqUUN3ME2wMlEhdk1? M1rhcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEGyPVMyLz5{M{SxNlk{OTxxYU6=
SEM-K2 NWewVIlJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfsO|IhcA>? NV3Sb2FrUUN3ME2wMlQhdk1? MnnNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MUK5N|EoRjJ|NEGyPVMyRC:jPh?=
RS4;11 NHjnPIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3YO|IhcA>? M2fYOmlEPTB-MUCsNFAxKG6P MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRzMkmzNUc,OjN2MUK5N|E9N2F-
THP-1 M3zWcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jmbVczKGh? MYjJR|UxRjFyLECwNEBvVQ>? NFTrfJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxNlk{OSd-MkO0NVI6OzF:L3G+
MV4-11 NUX1dGRzSXCxcITvd4l{KEG|c3H5 MmHwPE8zPCCq MmX0bY5lfWOnczDzbYdvcW[rY3HueEBidmRiZH;z[U1l\XCnbnTlcpQhWEGUUDDjcIVifmGpZTDhcoQh[WOldX31cIF1cW:wIH;mJJN2[i1{TjDEUmE> NGHrWpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxNlk{OSd-MkO0NVI6OzF:L3G+
MOLM-14 NGnpdZdCeG:ydH;zbZMhSXO|YYm= NETRWJM5NzJ2IHi= MlXwbY5lfWOnczDzbYdvcW[rY3HueEBidmRiZH;z[U1l\XCnbnTlcpQhWEGUUDDjcIVifmGpZTDhcoQh[WOldX31cIF1cW:wIH;mJJN2[i1{TjDEUmE> M2[zWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEGyPVMyLz5{M{SxNlk{OTxxYU6=
SEM-K2 MV;BdI9xfG:|aYOgRZN{[Xl? NIHaWHo5NzJ2IHi= MWfpcoR2[2W|IIPp[45q\mmlYX70JIFv\CCmb4PlMYRmeGWwZHXueEBRSVKSIHPs[YF3[WenIHHu[EBi[2O3bYXsZZRqd25ib3[gd5VjNTKQIFTORS=> NInz[II9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxNlk{OSd-MkO0NVI6OzF:L3G+
AML NIjKfmtEgXSxdH;4bYNqfHliYYPzZZk> NGexS45EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCVUxiY3XscJMhcXOxbHH0[YQh\nKxbTDy[YxieHOnZDDhZ5V1\SCveXXsc4llKGyndXvlcYliKHCjdHnlcpQhcGG{Yn;ybY5oKE[OVEOgTXRFKG23dHH0bY9vKGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFAxOyEQvF2u NEPR[Vk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[1OFQxQCd-MUm2OVQ1ODh:L3G+
MV4-11 M{LyXGN6fG:2b4jpZ4l1gSCjc4PhfS=> MlnaO|IhcHK| NITiO4JEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXjRvMUGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGOnbHygeIl1\XJvYnz1[UBie3OjeTygTWM2OCB;IECuNFAxPTZizszNMi=> NIrBXWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[1OFQxQCd-MUm2OVQ1ODh:L3G+
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RS4-11 MUDGeY5kfGmxbjDhd5NigQ>? M{[ydlIhcHK| MnjCTY5pcWKrdHnvckBw\iCITGSzJGlVTCCvdYThcpQh[XW2b4Doc5NxcG:{eXzheIlwdiCrbjDoeY1idiCUU{StNVEh[2WubIOgZYZ1\XJiMjDodpMh[nliZXzlZ5Rzd2OqZX3pcJVucW6nc3PlcoNmKGG|c3H5MEBKSzVyIE2gNE4xODFzIN88UU4> NFf4VYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[1OFQxQCd-MUm2OVQ1ODh:L3G+
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MV4-11 NHvTS3RHfW6ldHnvckBie3OjeR?= M4nweVExKG2pL3vn MYCyOEBpenN? M{Xld2lvKH[rdn:gbY5pcWKrdHnvckBw\iCITGSzJGlVTCCvdYThcpQhcW5iaIXtZY4hVVZ2LUGxJINmdGy|IIjlco9oemGodHXkJIlvKGG2aIntbYMhdnVxboWgcY92e2ViYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hW1SDVEWgdIhwe3Cqb4L5cIF1cW:wIHH0JHk3QTRiYYSgNVAhdWdxa3esJIlxKGGodHXyJFI1KGi{czDifUBqdW23bn;icI91KG2ndHjv[EBz\WyjdHn2[UB1dyClb370do9t NXPqdphVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2O|IxPDlpPkK5OlczODR7PD;hQi=>
MV4-11 Ml3OSpVv[3Srb36gZZN{[Xl? MXmwMlEhfU1? MkHZOEBpenN? MV7Jcohq[mm2aX;uJI9nKE[OVEOgbY4hcHWvYX6gUXY1NTFzIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBCU1RicHjvd5Bpd3K7bHH0bY9vKGG2IGPldlQ4OyC{ZYPp[JVmKGG2IECuNUB2VSCjZoTldkA1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh7NEm0OEc,Ojl6OUS5OFQ9N2F-
MV4-11 NH7UW3RHfW6ldHnvckBie3OjeR?= NYrRUWRkOC5zIIXN NFrGblY1KGi{cx?= M4L5XmlvcGmkaYTpc44hd2ZiRlzUN{BqdiCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFOWQWS1JJBpd3OyaH;yfYxifGmxbjDheEBVgXJ4OUSgdoV{cWS3ZTDheEAxNjFidV2gZYZ1\XJiNDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NH3i[GY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi5OFk1PCd-Mkm4PVQ6PDR:L3G+
MV4-11 MVPGeY5kfGmxbjDhd5NigQ>? NX7jSVJXOC5zIIXN NIX4cY81KGi{cx?= M1:wcmlvcGmkaYTpc44hd2ZiRlzUN{BqdiCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEWUS{GvNkBxcG:|cHjvdplt[XSrb36gZZQhXGi{MkCyM3R6ejJyNDDy[ZNq\HWnczDheEAxNjFidV2gZYZ1\XJiNDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz M1i1[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEm0PVQ1Lz5{OUi5OFk1PDxxYU6=
MV4-11 NIjxNG9HfW6ldHnvckBie3OjeR?= Mn7tNE4yKHWP NU\R[mZ1PCCqcoO= NEfHO|hKdmirYnn0bY9vKG:oIF\MWFMheGixc4Doc5J6dGG2aX;uJIF1KFS7ckW4PU82QTFicnXzbYR2\XNiaX6gbJVu[W5iTW[0MVEyKGOnbHzzJIF1KDBwMTD1UUBi\nSncjC0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NFTMbFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi5OFk1PCd-Mkm4PVQ6PDR:L3G+
Assay
Methods Test Index PMID
Western blot p-STAT5 / STAT5 / β-catenin / p-AKT / AKT / p-ERK / ERK / p-S6 / S6 ; phospho-FLT3 / FLT3 28625976 22875611
Immunofluorescence WGA / FLT3 28895560
Growth inhibition assay Cell viability 23967177
In vivo Oral administration of AC220 (10 mg/kg) induces time-dependent inhibition of FLT3 autophosphorylation in the FLT3-ITD–dependent MV4-11 tumor xenograft mouse model; the inhibition being 90% at 2 hours and 40% at 24 hours. AC220 significantly extends survival in a mouse model of FLT3-ITD AML with doses as low as 1 mg/kg given orally once a day. Treatment with AC220 at 10 mg/kg for 28 days results in rapid and complete regression of tumors in all mice with no tumor regrowth during the 60-day posttreatment period. AC220 displays more significant efficacy compared to sunitinib treatment which causes tumors to shrink slowly and resume growth immediately upon discontinuation of treatment in all but one of the mice. [1]

Protocol (from reference)

Kinase Assay:[1]
  • Inhibition of FLT3 autophosphorylation:

    To measure inhibition of FLT3 autophosphorylation, MV4-11 or RS4;11 cells are cultured in low serum media (0.5% FBS) overnight and seeded at a density of 400 000 cells per well in a 96-well plate the following day. The cells are incubated with different concentrations of AC220 for 2 hours at 37 °C. To induce FLT3 autophosphorylation in RS4;11 cells, 100 ng/mL FLT3 ligand is added for 15 minutes after the 2-hour AC220 incubation. Cell lysates are prepared and incubated in 96-well plates precoated with a total FLT3 capture antibody. The coated plates are incubated with either a biotinylated antibody against FLT3 to detect total FLT3 or an antibody against phosphotyrosines to detect FLT3 autophosphorylation. In both cases, a SULFO-tagged streptavidin secondary antibody is used for electrochemiluminescence detection on the Meso Scale Discovery platform. The concentration of AC220 that inhibits FLT3-ITD or TLT3-WT autophosphorylation by 50% represents IC50 value

Cell Research:[1]
  • Cell lines: MV4-11 and RS4;11 cells
  • Concentrations: Dissolved in DMSO, final concentration ~20 μM
  • Incubation Time: 72 hours
  • Method: Cells are cultured overnight in low serum media (0.5% FBS), seeded in a 96-well plate at 40 000 cells per well and exposed to AC220 for 72 hours at 37 °C. Cell viability is measured using the Cell Titer-Blue Cell Viability Assa
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: Female NU/NU or severe combined immunodeficient mice implanted with MV4-11 cells
  • Dosages: ~10 mg/kg
  • Administration: Oral gavage
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 33 mg/mL
(58.85 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
15% Captisol
For best results, use promptly after mixing.

30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 560.67
Formula

C29H32N6O4S

CAS No. 950769-58-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)(C)C1=CC(=NO1)NC(=O)NC2=CC=C(C=C2)C3=CN4C5=C(C=C(C=C5)OCCN6CCOCC6)SC4=N3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04459598 Completed Drug: Efavirenz|Drug: Quizartinib Healthy Subjects|Drug-drug Interaction|Pharmacokinetics|Quizartinib Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. September 8 2020 Early Phase 1
NCT04473664 Completed Drug: Quizartinib Hepatic Impairment|Moderate Impaired Hepatic Function Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. September 29 2020 Phase 1
NCT04459585 Completed Drug: Dabigatran Etexilate Mesylate|Drug: Quizartinib Healthy Subjects|Drug-drug Interaction|Pharmacokinetics|Quizartinib Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. August 28 2020 Early Phase 1
NCT04209725 Terminated Drug: CPX-351|Drug: Quizartinib Leukemia Myeloid Acute SCRI Development Innovations LLC June 3 2020 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Is it possible to alter the captisol concentration to make it more dissolvable i.e 20% or 25% captisol ?

Answer:
In 15% Captisol, the compound forms s suspension at 30mg/ml. Increasing the percentage of Captisol will not convert the mixture into solution. You can use suspension for oral gavage feeding.

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