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(+)-JQ1

For research use only.

Catalog No.S7110

261 publications

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.

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Selleck's (+)-JQ1 has been cited by 261 publications

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Choose Selective Target Protein Ligand Inhibitors

Biological Activity

Description (+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.
Features (+)-JQ1 is more effective than (-)-JQ1.
Targets
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
In vitro

(+)-JQ1 enantiomer binds directly into the Kac binding site of BET bromodomains. (+)-JQ1 (500 nM) binds BRD4 competitively with chromatin resulting in differentiation and growth arrest of NMC cells. (+)-JQ1 (500 nM) attenuates rapid proliferation of NMC 797 and Per403 cell lines as demonstrated by reduced Ki67 staining. (+)-JQ1 (500 nM) potently decreases expression of both BRD4 target genes in NMC 797 cells. (+)-JQ1 inhibits cellular viability with IC50 of 4 nM in NMC 11060 cells. [1] (+)-JQ1 results in robust inhibition of MYC expression in MM cell lines. (+)-JQ1 inhibits proliferating of KMS-34 and LR5 with IC50 of 68 nM and 98 nM, respectively. (+)-JQ1 (500 nM)-treated MM.1S cells results in a pronounced decrease in the proportion of cells in S-phase, with a concomitant increase in cells arrested in G0/G1. (+)-JQ1 (500 nM) results in pronounced cellular senescence by beta-galactosidase staining. (+)-JQ1 (800 nM) exposure leads to a significant reduction in cell viability among the majority of CD138+ patient-derived MM samples tested. [2] (+)-JQ1 inhibits growth of LP-1 cells with GI50 of 98 nM. (+)-JQ1 (625 nM) results in an increase in the percentage of LP-1 cells in G0/G1. (+)-JQ1 (500 nM) suppresses the expression of MYC, BRD4 and CDK9 in LP-1 cells. [3] (+)-JQ1 (1 μM) activates HIV transcription in latently infected Jurkat T cells. (+)-JQ1 (50 μM) stimulates predominantly Tat-dependent HIV transcription in both Jurkat and HeLa cells. (+)-JQ1 (5 μM) induces Brd4 dissociation enables Tat to recruit SEC to HIV promoter and induce Pol II CTD phosphorylation and viral transcription in J-Lat A2 cells. JQ1 enables Tat to increase CDK9 T-loop phosphorylation and partially dissociates P-TEFb from 7SK snRNP in Jurkat T cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MkXnR4VtdCCYaXHibYxqfHliQYPzZZk> MmPQNlUxNzVyMD:xNFAxKG6P NVnlVHVWOjRxNEivO|IhcA>? NW\n[XNWTE2VTx?= MknXbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=> M1vHSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP NYrJZYk2S2WubDDWbYFjcWyrdImgRZN{[Xl? M{PNTlI2OC93MECvNVAxOCCwTR?= NGHGXowzPC92OD:3NkBp M4PzdWROW09? NIrBUWZqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIHTvd4UuKGGwZDD0bY1mNSCmZYDlcoRmdnRibXHucoVz NYDJdVFIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFc5QDFpPkK2O|A4QDhzPD;hQi=>
K1  Mof1R4VtdCCFeXPs[UBCe3OjeR?= NVjYW5psOjVyL{WwNE8yODByIH7N Mof3O|IhcA>? NGLReodFVVOR NXTOUY9y[XK{ZYP0d{Bk\WyuIHP5Z4xmKGG2IFewM2cyKHCqYYPl MnXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEe4PFEoRjJ4N{C3PFgyRC:jPh?=
BCPAP MkDMR4VtdCCFeXPs[UBCe3OjeR?= MoG4NlUxNzVyMD:xNFAxKG6P M3zUWFczKGh? MXzEUXNQ NHPjWZVienKnc4TzJINmdGxiY4njcIUh[XRiR{CvS|EheGijc3W= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
Hep3B M3;yNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLoNE0yOCEQvF2= NHzjUGg2KGR? NFrE[4tFVVOR NESyTmRKSzVyPUCuNFgh|ryP MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HCCLM3 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWn3TGx[OC1zMDFOwG0> MUm1JIQ> MnzqSG1UVw>? NFHISpVKSzVyPUCuNVQh|ryP NYfl[ZVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HuH7 NYTWO2ZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LXOVAuOTBizszN Mly4OUBl MkjoSG1UVw>? MoTiTWM2OD1yLkKxJO69VQ>? MlPRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HepG2 NEjSO|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\u[ollOC1zMDFOwG0> MkjsOUBl NIn3dYRFVVOR NGfQZpVKSzVyPUCuN|Qh|ryP NX\IO3l3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
SMMC7721 NHj0eWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYGwMVExKM7:TR?= MY[1JIQ> MlnYSG1UVw>? MmfCTWM2OD1yLkSxJO69VQ>? NEPMOoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
BEL7402 M1rRPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUiwMVExKM7:TR?= NF7ieWQ2KGR? NIn2PIlFVVOR Ml3kTWM2OD1yLkS3JO69VQ>? M2nDVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
MHCC97H NUWwR2dET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NECxOXkxNTFyIN88US=> Mo[xOUBl NXz3cGVrTE2VTx?= MWPJR|UxRTBwNEGg{txO NXTreIR5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
Hep3B MVjD[YxtKEO7Y3zlJGF{e2G7 MkPwNE4yNzBwNT:yMlUh|ryP NXGyOop2PDhiaB?= NVH5TW55TE2VTx?= MXTs[YFleyC2bzDhJJN2[nO2YX70bYFtKGGlY4XteYxifGmxbjDv[kBJS0NiY3XscJMhcW5ic4XiMWcyKHCqYYPlxsA> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HCCLM3 MmjXR4VtdCCFeXPs[UBCe3OjeR?= Mnu0NE4yNzBwNT:yMlUh|ryP MnzPOFghcA>? NInvW|dFVVOR MXns[YFleyC2bzDhJJN2[nO2YX70bYFtKGGlY4XteYxifGmxbjDv[kBJS0NiY3XscJMhcW5ic4XiMWcyKHCqYYPlxsA> NHy2Zms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
Hep3B MUTBdI9xfG:|aYOgRZN{[Xl? MX2wMlEwOC53L{KuOUDPxE1? NEnyT4s1QCCq MonFSG1UVw>? NF70TJFi[3SrdnH0[ZMh[2G|cHHz[U0{KGGwZDDjZZNx[XOnLUmg[ZhxemW|c3nvckBidmRiaX7keYNm\CCSQWLQJINt\WG4YXflJIF{KHenbHygZZMh[3m2b3Podo9u\SClIILlcIVie2ViaX70c{B1cGViY4n0c5Bt[XOvIH\yc40hdWm2b3Poc45lemmj NEDBS2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HCCLM3 M1\JeWFxd3C2b4Ppd{BCe3OjeR?= M1O2WlAvOS9yLkWvNk42KM7:TR?= MlrUOFghcA>? MoHWSG1UVw>? M3Psd4FkfGm4YYTld{Bk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOTDlfJBz\XO|aX;uJIFv\CCrbnT1Z4VlKFCDUmCgZ4xm[X[jZ3WgZZMhf2WubDDhd{BkgXSxY3jyc41mKGNicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21iZoLvcUBucXSxY3jvcoRzcWF? M3HFWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
A549 M4TWWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPONE4yNTFyIN88US=> MmfJO|IhcA>? NGTQVotqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M2jhO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H157 NV;rPFNrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLKS4kxNjFvMUCg{txO Mn\pO|IhcA>? M2LKeolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M{LV[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H1299 MmKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWf3T4NXOC5zLUGwJO69VQ>? NWfFeYdZPzJiaB?= MXzpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NHzlSlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
A549 NF7iSGVHfW6ldHnvckBCe3OjeR?= M{ny[|EwOi53L{Wg{txO NV\0W2xjOTJiaB?= M1vqUOKhf2Wja3z5JIRm[3KnYYPl[EBD[2xvMjDs[ZZmdHN? M1nPXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H1299 MYHGeY5kfGmxbjDBd5NigQ>? MYexM|IvPS93IN88US=> M1\2NlEzKGh? MoL2xsB4\WGtbImg[IVkemWjc3XkJGJkdC1{IHzleoVtew>? M{HvTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H157 MYnGeY5kfGmxbjDBd5NigQ>? M2e1SlEwOi53L{Wg{txO NH7Yb2kyOiCq MUnk[YNz\WG|ZXSgSHI1KGW6cILld5Nqd25? NETYZlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
H1299 NVfhUXNyTnWwY4Tpc44hSXO|YYm= MkPqNU8zNjVxNTFOwG0> NFXtRZMyOiCq MlzZ[IVkemWjc3XkJGRTPCCneIDy[ZN{cW:w MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
C8161 NEH1endE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NX63XI46OC1{IN88US=> NIPMSng1KGR? MnPISG1UVw>? NVXDS3hr\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M2\F[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel285 MoCxR4VtdCCYaXHibYxqfHliQYPzZZk> NU\TR4lrOC1{IN88US=> MUS0JIQ> NHy3[Y1FVVOR M4TIdYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NFrpfZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Mel290 NVzlfZJyS2WubDDWbYFjcWyrdImgRZN{[Xl? Mn;HNE0zKM7:TR?= M{PYeVQh\A>? MXrEUXNQ Ml30[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
92.1 MV;D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MnnFNE0zKM7:TR?= MnzsOEBl M4jZc2ROW09? NWHkdJBH\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy Mn3wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Omm1.3 NYDq[FJRS2WubDDWbYFjcWyrdImgRZN{[Xl? MUiwMVIh|ryP NHfHRVI1KGR? NIr3b3FFVVOR MmPv[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MofYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel202 NGT5dHdE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NVzXNXpIOC1{IN88US=> NYfJclB2PCCm MYjEUXNQ MYHk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NULLPG01RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Mel270 Mn3jR4VtdCCYaXHibYxqfHliQYPzZZk> MX:wMVIh|ryP NE\URWs1KGR? NFzWVpZFVVOR MUTk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M3zn[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Omm1 NH;xb4pE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MX2wMVIh|ryP MlzOOEBl MlzpSG1UVw>? NUHKPZZL\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NX7OV2dxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
92.1 MoLJRZBweHSxc3nzJGF{e2G7 MX21NFAhdk1? M{DNU|Q5KGh? NE\GTlhFVVOR MWTpcoR2[2W|IHHwc5B1d3Orcx?= NUi0dlZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1.3 MnPBRZBweHSxc3nzJGF{e2G7 M1\iOFUxOCCwTR?= MnvjOFghcA>? M1vUcGROW09? NGLpdGVqdmS3Y3XzJIFxd3C2b4Ppdy=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
92.1 NXfvcJdUS2WubDDDfYNt\SCDc4PhfS=> NX2xdHJ5PTByIH7N MmH1NlQwPDhxN{KgbC=> M{O0T2ROW09? NH7RRWFqdmS3Y3XzJJRp\SClZXzsJIFk[3WvdXzheIlwdiCjdDDzeYIuTzIEoB?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Omm1.3 M1TLcWNmdGxiQ4njcIUhSXO|YYm= NXr1WmhiPTByIH7N MUiyOE81QC95MjDo M2jpZWROW09? MWXpcoR2[2W|IITo[UBk\WyuIHHjZ5VufWyjdHnvckBifCC|dXKtS|HDqA>? NFPWOG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
A549 MlvTSpVv[3Srb36gRZN{[Xl? MmrzNVAxNzRyMD:xNFAxKG6P MY[yOEBp NHfVT4p2eHKnZ4XsZZRmeyCjbnSgZYN1cX[jdHXzJHNKWlRz MkjtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{MUKxPVkoRjJ4MkGyNVk6RC:jPh?=
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MV4-11 MmrsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVm3NkBp NFLNRY1KSzVyIE2gNE4xOjNizszN NVrhPIJ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
MV4-11 M1\veWN6fG:2b4jpZ4l1gSCjc4PhfS=> NV\DTmN7PCCmYYnz MmraTWM2OCB;IECuNFI1KM7:TR?= NIHpVpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
MV4-11 NFPnWZpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVK0JIRigXN? NVHsUWhSUUN3MDC9JFAvODJ2IN88US=> NWPaT29oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 M2O2Z2Z2dmO2aX;uJIF{e2G7 NWH0OotbOzBibXnudy=> Mn7STWM2OCB;IECuNFI5PyEQvF2= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
NALM16 NFrWdWVEgXSxdH;4bYNqfHliYYPzZZk> MW[1JIRigXN? NF[3N2lGSzVyIE2gNE4xOyEQvF2= NG[2PWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
BL21(DE3)-R3-pRARE2 NET0WJpHfW6ldHnvckBie3OjeR?= NEe3Wmg{OCCvaX7z NXe2UFduUUN3MDC9JFAvODN|IN88US=> NFHnelE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG5OVczOyd-MkixPVU4OjN:L3G+
BL21(DE3) Mlj2SpVv[3Srb36gZZN{[Xl? NX7I[WxXU2RiPTCwMlA{PCEQvF2= M1T2UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
Rosetta2 DE3 NG[5VHVHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 NUjkNpA{OzBibXnudy=> M4Hrd2lEPTBiPTCwMlA{PTdizszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 M2TaR2ZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? MnuzN|AhdWmwcx?= M{PhcmlEPTBiPTCwMlA1OjJizszN M2q3cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
Rosetta2 DE3 MUjGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 NF62clg{OCCvaX7z Ml;sTWM2OCB;IECuNFQ3PyEQvF2= NXvReJZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
MOLM13 NX;QNVZIS3m2b4TvfIlkcXS7IHHzd4F6 NFvzclY1KGSjeYO= M372VGlEPTBiPTCwMlA2PiEQvF2= NHvqeZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
MOLM13 NEXL[mFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1HTR|Qh\GG7cx?= MoPGTWM2OCB;IECuNFU3KM7:TR?= M{DPbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
HL60 NFPKeVhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3O4XVczKGh? M4DnWWlEPTBiPTCwMlA3KM7:TR?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
HL60 NXPteZVyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUKzJIRigXN? NY\DSJQ4T0N3MDC9JFAvODZizszN M1mzV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkW3NFk6Lz5{OU[1O|A6QTxxYU6=
NALM6 MojJR5l1d3SxeHnjbZR6KGG|c3H5 MY[1JIRigXN? NVuwNYd4TUN3MDC9JFAvODZizszN NEPmUVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+
Raji MnvVSpVv[3Srb36gZZN{[Xl? NXnuOJNFUUN3MDC9JFAvODZizszN NV\pN3BuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
Raji NUizS5lKTnWwY4Tpc44h[XO|YYm= M1PKXVQhcA>? MljVTWM2OCB;IECuNFY6KM7:TR?= MkTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3OVgoRjJ2OUCwO|U5RC:jPh?=
MM1S MlnsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NWjsdJVKPzJiaB?= NUThfplpUUN3MDC9JFAvODZ7MTFOwG0> M37p[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUK1OFM2Lz5{OUWyOVQ{PTxxYU6=
BL21 (DE3)-codon plus-RIL MYHGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 Mn:zZpkh\my3b4Lld4NmdmOnIHHubZNwfHKxcImgZZN{[Xl? NEXGOHBKSzVyIE2gNE4xPyEQvF2= NWnjTWJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1PFY4OThpPkK4OVg3PzF6PD;hQi=>
22Rv1 Mke2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NFHqOI46PiCq Ml\ETWM2OCB;IECuNFcyKM7:TR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
22Rv1 MnjFRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M4T5eFEzKGh? NX\iWJVwUUN3MDC9JFAvODdzIN88US=> M4HzWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
MV4-11 MmXwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnLsTWM2OCB;IECuNFczKM7:TR?= NYLUT5JvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPVU4OjNpPkK4NVk2PzJ|PD;hQi=>
BL21(DE3)-R3-pRARE2 Mo[0SpVv[3Srb36gZZN{[Xl? M2H4eVMxKG2rboO= MULJR|UxKD1iMD6wO|ch|ryP NUn6RotzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
BL21(DE3)-R3-pRARE2 MWnGeY5kfGmxbjDhd5NigQ>? MY[zNEBucW6| M1r5TWlEPTBiPTCwMlA4PyEQvF2= NFLBeGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG5OVczOyd-MkixPVU4OjN:L3G+
BL21(DE3)-R3-pRARE2 NVSyN2k1TnWwY4Tpc44h[XO|YYm= M{Xn[VMxKG2rboO= MX;JR|UxKD1iMD6wO|ch|ryP NV3vdYtlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3O|Y5OzRpPkK5O|c3QDN2PD;hQi=>
MV4-11 NY[1T3c{S3m2b4TvfIlkcXS7IHHzd4F6 MXmyOEBp MYrHTVUxKD1iMD6wPEDPxE1? NVSy[5pxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVE{PjNpPkK2NVkyOzZ|PD;hQi=>
MV411 MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXLGeWV4PzJiaB?= NX7jbpd1UUN3MDC9JFAvODhizszN MnnqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh|MUS1NVMoRjJ6M{G0OVE{RC:jPh?=
TY82 MVvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NFy4SZE4OiCq MlX6TWM2OCB;IECuNFgxQCEQvF2= MkXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3MkW0N|UoRjJ7NUK1OFM2RC:jPh?=
HL60 MWDGeY5kfGmxbjDhd5NigQ>? MWSyOEBp NFLMe4pKSzVyIE2gNE4xQDZizszN NX\FW4l1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
697 MX7DfZRwfG:6aXPpeJkh[XO|YYm= NGnocG42KGSjeYO= NVPOeGZXTUN3MDC9JFAvODlizszN MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
Loucy Mlz4R5l1d3SxeHnjbZR6KGG|c3H5 MX61JIRigXN? NWT6U3czTUN3MDC9JFAvODlizszN MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
BL21(DE3) MYrGeY5kfGmxbjDhd5NigQ>? MYLL[EA:KDBwMEmyJO69VQ>? M{\IPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
BL21(DE3)-R3-pRARE2 MWDGeY5kfGmxbjDhd5NigQ>? NFLhd25M\CB;IECuNUDPxE1? Ml3oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OUWwNFcoRjJ6NUm1NFA4RC:jPh?=
HT-29 M{DIS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWX2ZnRFPzJiaB?= NXe1Z21PUUN3MDC9JFAvOTB2IN88US=> NVPKVIk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
MM1S MlmwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXG3NkBp M1;rPWlEPTBiPTCwMlExQSEQvF2= M3PtOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW5OFI5Lz5{NUW1PVQzQDxxYU6=
LNCAP cells MnrSRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NVXIeVZkUUN3MDC9JFAvOTB7NjFOwG0> M4nnU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
T cells MXLGeY5kfGmxbjDhd5NigQ>? NEL5V2gzPCCq NYm2eYRyUUN3MDC9JFAvOTFizszN NFuyN5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
HL60 MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NEiybnE4OiCq NUDZO4s3UUN3MDC9JFAvOTFizszN NEPEWGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki2PVE6PCd-Mk[4OlkyQTR:L3G+
BL21(DE3) NFzzOoNHfW6ldHnvckBie3OjeR?= NIT3XnJKSzVyIE2gNE4yOiEQvF2= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
BL21(DE3) NFjUcFFHfW6ldHnvckBie3OjeR?= NVHhfYNlOi53IHi= NEnDZppKSzVyIE2gNE4yOiEQvF2= NWDxWGt3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
H1299 M1vQSWZ2dmO2aX;uJIF{e2G7 MXOyOEBp NW[xfFlZTUN3MDC9JFAvOTV|IN88US=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl2OUWyNUc,Ojh7NEm1NlE9N2F-
HD-MB03 NIXQUoFEgXSxdH;4bYNqfHliYYPzZZk> M1nqcFUh\GG7cx?= M{LmZWVEPTBiPTCwMlE3KM7:TR?= NWTodWRYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
LNCAP NGf5U2lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MnfmPVYhcA>? M{PYR2lEPTBiPTCwMlE3KM7:TR?= NYG0[XpVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
Hs578T NH:0PG9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NF;kbJEyOiCq Mo\FTWM2OCB;IECuNVYh|ryP NYD5XpJoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MV4-11 MmnjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M37wWVEzKGh? NIXJSI5KSzVyIE2gNE4yPiEQvF2= NFfwZWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
LNCAP NY[5fZVySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MlvQNVIhcA>? NWDiflZbUUN3MDC9JFAvOTZizszN NFTST2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
C4-2B M2rFNmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3HtZVk3KGh? Mlz3TWM2OCB;IECuNVkh|ryP NGH5W3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
C4-2B NVnPTnp1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NHvCbnAyOiCq MXPJR|UxKD1iMD6xPUDPxE1? NV3RXXFIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
Vero E6 NVP0c4VDSW62aY\pdoFtKGGldHn2bZR6KGG|c3H5 MV:0PEBp MmjiTWM2OCB;IECuNVkzPzVizszN Mm\vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzJ|NUO4OVkoRjN{M{WzPFU6RC:jPh?=
MCF7 NFz6OFVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYPhOZl2OTJiaB?= M3vheWlEPTBiPTCwMlIh|ryP NXLkd2lZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MV4-11 NWHHU2NLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MW\JR|UxKD1iMD6yOEDPxE1? Ml;XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNEK3OVEoRjJ5MUSyO|UyRC:jPh?=
MV4-11 Mkm5R5l1d3SxeHnjbZR6KGG|c3H5 M1\M[lczKGh? NGTmcHNKSzVyIE2gNE4zPDJizszN NXrSUWNURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NVcxOTFpPkKzOVE4ODFzPD;hQi=>
MX1 NIn0OIZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3\XV|czKGh? NIXuXZlGSzVyIE2gNE4zPTRizszN NV7mbZA2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFk2OjFpPkK4PVQ6PTJzPD;hQi=>
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MDA-MB-231 NEOwXWxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoXEN{Bl[Xm| MorqS2M2OCB;IECuN{DPxE1? NUHEbZVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
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SAE MlrvSpVv[3Srb36gZZN{[Xl? Mnj0OEBp M33MOWlEPTBiPTCxMlQ6KM7:TR?= NGK2PHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0PVc1OSd-Mkm2OFk4PDF:L3G+
SAE M1HWPGZ2dmO2aX;uJIF{e2G7 MlmyOEBp NXHq[WROUUN3MDC9JFEvPTFizszN NVzPeYxvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
U2OS NEf0cVVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYGxNkBp NXrHSYxMUUN3MDC9JFEvPjJizszN M2PkTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
SAE MV7GeY5kfGmxbjDhd5NigQ>? NIniZ3U1KGh? MYDJR|UxKD1iMT62N{DPxE1? NGj1Z|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0PVc1OSd-Mkm2OFk4PDF:L3G+
A549 MmLGRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M4L2PFEzKGh? MX3JR|UxKD1iMT62O{DPxE1? MortQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MCF7 NXfodGcyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYKzJIRigXN? NXn6eHVqT0N3MDC9JFEvPyEQvF2= M2D0VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
DU145 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NEjRSVA6PiCq NUHacJltUUN3MDC9JFIvPTJizszN MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
DU145 Mn\BRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NFzncYEyOiCq NGHN[49KSzVyIE2gNk42OiEQvF2= NFzHeXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
T47D NGLUXGlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mkn6N{Bl[Xm| NHv3cGhISzVyIE2gNk45KM7:TR?= NXnU[YZCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
PC3 MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXjjVHpwQTZiaB?= NGLVPW9KSzVyIE2gN{4xOSEQvF2= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
PC3 NVTlNFlxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NGLNPW8yOiCq M3\v[mlEPTBiPTCzMlAyKM7:TR?= NVzONIN[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
HeLa M3HOSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NEG0[5QyOiCq NYr0codtUUN3MDC9JFMvPzZizszN NXS3NY9ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
K562 M2\RNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2jLdlMh\GG7cx?= NELrOHFISzVyIE2gN{45KM7:TR?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
A2780 MXnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoHSN{Bl[Xm| NUm3dXNiT0N3MDC9JFQh|ryP MmfVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
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K562 MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Ml\uO|IhcA>? NWHkRoxSUUN3MDC9JFkvOTJizszN MlT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh|MUS1NVMoRjJ6M{G0OVE{RC:jPh?=
OVCAR5 NGXkZ5pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnnVN{Bl[Xm| NELV[GpISzVyIE2gNVIh|ryP MnuyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
In vivo (+)-JQ1 (50 mg/kg) inhibits tumors growth in mice with NMC 797 xenografts. (+)-JQ1 (50 mg/kg) results in effacement of NUT nuclear speckles in mice with NMC 797 xenografts, consistent with competitive binding to nuclear chromatin. (+)-JQ1 (50 mg/kg) induces strong (grade 31) keratin expression in NMC 797 xenografts. (+)-JQ1 (50 mg/kg) promotes differentiation, tumor regression and prolonged survival in mice models of NMC xenografts. [1] (+)-JQ1 (50 mg/kg) results in a significant prolongation in overall survival of SCID-beige mice orthotopically xenografted after intravenous injection with MM.1S-luc+ cells compared to vehicle-treated animals. [2] (+)-JQ1 (50 mg/kg i.p.) leads to a highly significant increase in survival of mice bearing Raji xenografts. [3]

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: MC 11060 cells
  • Concentrations: ~500 nM
  • Incubation Time: 48 hours
  • Method:

    Cells are seeded into white, 384-well microtiter plates at 500 cells per well in a total volume of 50 μL media. The 797, TT and TE10 cells are grown in DMEM containing 1% penicillin/streptomycin and 10% FBS. The Per403 cells are grown in DMEM containing 1 % penicillin/streptomycin and 20% FBS. Patient-derived NMC 11060 cells are grown in RPMI with 10% FBS and 1% penicillin/streptomycin. (+)-JQ1 is delivered to microtiter assay plates by robotic pin transfer. Following a 48 hours incubation at 37℃, cells are lysed and wells are assessed for total ATP content using a commercial proliferation assay. Replicate measurements are analyzed with respect to dose and estimates of IC50 are calculated by logistic regression (GraphPad Prism).


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: Mice bearing NMC 797 xenografts
  • Dosages: 50 mg/kg
  • Administration: intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 456.99
Formula

C23H25ClN4O2S

CAS No. 1268524-70-4
Storage powder
in solvent
Synonyms N/A
Smiles CC1=C(SC2=C1C(=NC(C3=NN=C(N32)C)CC(=O)OC(C)(C)C)C4=CC=C(C=C4)Cl)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    How can I reconstitute the compound for in vivo injection?

  • Answer:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID