(+)-JQ1

Catalog No.S7110

For research use only.

(+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

Selleck's (+)-JQ1 has been cited by 311 publications

Purity & Quality Control

Choose Selective Target Protein Ligand Inhibitors

Biological Activity

Description (+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.
Features (+)-JQ1 is more effective than (-)-JQ1.
Targets
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
In vitro

(+)-JQ1 enantiomer binds directly into the Kac binding site of BET bromodomains. (+)-JQ1 (500 nM) binds BRD4 competitively with chromatin resulting in differentiation and growth arrest of NMC cells. (+)-JQ1 (500 nM) attenuates rapid proliferation of NMC 797 and Per403 cell lines as demonstrated by reduced Ki67 staining. (+)-JQ1 (500 nM) potently decreases expression of both BRD4 target genes in NMC 797 cells. (+)-JQ1 inhibits cellular viability with IC50 of 4 nM in NMC 11060 cells. [1] (+)-JQ1 results in robust inhibition of MYC expression in MM cell lines. (+)-JQ1 inhibits proliferating of KMS-34 and LR5 with IC50 of 68 nM and 98 nM, respectively. (+)-JQ1 (500 nM)-treated MM.1S cells results in a pronounced decrease in the proportion of cells in S-phase, with a concomitant increase in cells arrested in G0/G1. (+)-JQ1 (500 nM) results in pronounced cellular senescence by beta-galactosidase staining. (+)-JQ1 (800 nM) exposure leads to a significant reduction in cell viability among the majority of CD138+ patient-derived MM samples tested. [2] (+)-JQ1 inhibits growth of LP-1 cells with GI50 of 98 nM. (+)-JQ1 (625 nM) results in an increase in the percentage of LP-1 cells in G0/G1. (+)-JQ1 (500 nM) suppresses the expression of MYC, BRD4 and CDK9 in LP-1 cells. [3] (+)-JQ1 (1 μM) activates HIV transcription in latently infected Jurkat T cells. (+)-JQ1 (50 μM) stimulates predominantly Tat-dependent HIV transcription in both Jurkat and HeLa cells. (+)-JQ1 (5 μM) induces Brd4 dissociation enables Tat to recruit SEC to HIV promoter and induce Pol II CTD phosphorylation and viral transcription in J-Lat A2 cells. JQ1 enables Tat to increase CDK9 T-loop phosphorylation and partially dissociates P-TEFb from 7SK snRNP in Jurkat T cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MlTTR4VtdCCYaXHibYxqfHliQYPzZZk> MlHMNlUxNzVyMD:xNFAxKG6P NG\rd5EzPC92OD:3NkBp NGDrUXFFVVOR Mm[4bY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
BCPAP NH\ab4RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M4\mV|I2OC93MECvNVAxOCCwTR?= M33RfVI1NzR6L{eyJIg> Mke3SG1UVw>? NVTsNXVCcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgbY4h[m:2aDDkc5NmNSCjbnSgeIlu\S1iZHXw[Y5l\W62IH3hco5meg>? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
K1  MUjD[YxtKEO7Y3zlJGF{e2G7 M{[3W|I2OC93MECvNVAxOCCwTR?= MlzTO|IhcA>? NEXNd2NFVVOR M{S5boFzemW|dIOgZ4VtdCCleXPs[UBifCCJMD;HNUBxcGG|ZR?= M{XqcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP M3PHfWNmdGxiQ4njcIUhSXO|YYm= MnfONlUxNzVyMD:xNFAxKG6P NFHJZ4g4OiCq NYLRUXZpTE2VTx?= M{Ps[YFzemW|dIOgZ4VtdCCleXPs[UBifCCJMD;HNUBxcGG|ZR?= NVf6OWdYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFc5QDFpPkK2O|A4QDhzPD;hQi=>
Hep3B NYjUVo85T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXmwMVExKM7:TR?= MoC5OUBl M2DwWmROW09? M4nJd2lEPTB;MD6wPEDPxE1? M{DkNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HCCLM3 MlPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW[wMVExKM7:TR?= NYflc5A1PSCm NFX0NoRFVVOR MXTJR|UxRTBwMUSg{txO MnrBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HuH7 NID6NWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnu3NE0yOCEQvF2= MoLTOUBl MmnKSG1UVw>? MULJR|UxRTBwMkGg{txO MlnjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HepG2 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjIOI1wOC1zMDFOwG0> NGHxTpE2KGR? NGjGS4FFVVOR M1P3N2lEPTB;MD6zOEDPxE1? Mn;VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
SMMC7721 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVuwMVExKM7:TR?= MoDFOUBl M2i3PWROW09? NYrufml5UUN3ME2wMlQyKM7:TR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
BEL7402 M3LuXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPDb3FUOC1zMDFOwG0> NFjobpg2KGR? NEnLUlBFVVOR NYD6ZlNPUUN3ME2wMlQ4KM7:TR?= NHfpRm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
MHCC97H NVvWRmNKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljMNE0yOCEQvF2= NXTafXE{PSCm NV3qbnNzTE2VTx?= MXvJR|UxRTBwNEGg{txO M3zFOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
Hep3B NHvIUnpE\WyuIFP5Z4xmKEG|c3H5 MYOwMlEwOC53L{KuOUDPxE1? M1ftdFQ5KGh? NF3wbJRFVVOR Ml;rcIVi\HNidH:gZUB{fWK|dHHueIlidCCjY3P1cZVt[XSrb36gc4YhUEOFIHPlcIx{KGmwIIP1Zk1IOSCyaHHz[eKh MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HCCLM3 MlXqR4VtdCCFeXPs[UBCe3OjeR?= MVuwMlEwOC53L{KuOUDPxE1? M1zFOVQ5KGh? MXXEUXNQ M4i3eoxm[WS|IITvJIEhe3Wkc4ThcpRq[WxiYXPjeY12dGG2aX;uJI9nKEiFQzDj[YxteyCrbjDzeYIuTzFicHjhd4XDqA>? NGLTN3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
Hep3B M3Xkc2Fxd3C2b4Ppd{BCe3OjeR?= M2nPXFAvOS9yLkWvNk42KM7:TR?= MW[0PEBp MWfEUXNQ M2T5V4FkfGm4YYTld{Bk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOTDlfJBz\XO|aX;uJIFv\CCrbnT1Z4VlKFCDUmCgZ4xm[X[jZ3WgZZMhf2WubDDhd{BkgXSxY3jyc41mKGNicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21iZoLvcUBucXSxY3jvcoRzcWF? M1zoeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HCCLM3 MUXBdI9xfG:|aYOgRZN{[Xl? NY\1[XJxOC5zL{CuOU8zNjVizszN NV7TZpZrPDhiaB?= Ml\4SG1UVw>? MoHVZYN1cX[jdHXzJINie3Cjc3WtN{BidmRiY3HzdIF{\S17IHX4dJJme3Orb36gZY5lKGmwZIXj[YQhWEGUUDDjcIVifmGpZTDhd{B4\WyuIHHzJIN6fG:laILvcYUh[yC{ZXzlZZNmKGmwdH:geIhmKGO7dH;wcIF{dSCocn;tJI1qfG:laH;u[JJq[Q>? M3X4dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
A549 MknqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYSwMlEuOTBizszN MkjCO|IhcA>? MVjpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MmHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H157 NFfrXWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPMR2oxNjFvMUCg{txO M3HIV|czKGh? NWDaXFdPcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NHqwUG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
H1299 NULNV5k6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\FdWExNjFvMUCg{txO M1nyV|czKGh? NHzmfnVqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M{HpSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
A549 MUPGeY5kfGmxbjDBd5NigQ>? NECwNVAyNzJwNT:1JO69VQ>? NEntfW0yOiCq MXJCpJdm[WuueTDk[YNz\WG|ZXSgRoNtNTJibHX2[Yx{ NYLGV4Z5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 MYLGeY5kfGmxbjDBd5NigQ>? MV2xM|IvPS93IN88US=> M2K0WlEzKGh? NEDvdZPDqHenYXvsfUBl\WO{ZXHz[YQhSmOuLUKgcIV3\Wy| MkDvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H157 Ml\MSpVv[3Srb36gRZN{[Xl? MVexM|IvPS93IN88US=> M3exeFEzKGh? MWrk[YNz\WG|ZXSgSHI1KGW6cILld5Nqd25? NInoRog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
H1299 MkfRSpVv[3Srb36gRZN{[Xl? NGTnbFQyNzJwNT:1JO69VQ>? MVKxNkBp Ml;y[IVkemWjc3XkJGRTPCCneIDy[ZN{cW:w NETwe|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
C8161 MmjhR4VtdCCYaXHibYxqfHliQYPzZZk> NVXUPVBLOC1{IN88US=> NGWzPVI1KGR? M4jZNGROW09? NHiwUpZl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M4fS[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel285 M{joRWNmdGxiVnnhZoltcXS7IFHzd4F6 NIjNU4oxNTJizszN MXe0JIQ> MoTPSG1UVw>? NILKUodl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M1voUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel290 M4P2WGNmdGxiVnnhZoltcXS7IFHzd4F6 M1roZ|AuOiEQvF2= NY\xTnBLPCCm M2nnfGROW09? MYXk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
92.1 NHziSmVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NI\XVmYxNTJizszN M4HQUFQh\A>? M2\5c2ROW09? NIX0e2ll\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NXLjZW5wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1.3 NE\abG1E\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1jsZVAuOiEQvF2= MorGOEBl NV\i[GUxTE2VTx?= M4XEUoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Mel202 NV\BdVBxS2WubDDWbYFjcWyrdImgRZN{[Xl? M4rJZVAuOiEQvF2= MYe0JIQ> MUHEUXNQ MUPk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Mel270 MYnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmToNE0zKM7:TR?= NWPQW4ZPPCCm Mmr2SG1UVw>? Mn7Z[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NYXFTnE6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1 M{nXV2NmdGxiVnnhZoltcXS7IFHzd4F6 NX;nbpNNOC1{IN88US=> MonNOEBl M3u2SWROW09? NUD1Zol3\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
92.1 NX;WZXo2SXCxcITvd4l{KEG|c3H5 MUG1NFAhdk1? NVHVeoVJPDhiaB?= M1Pq[mROW09? MVrpcoR2[2W|IHHwc5B1d3Orcx?= MnnHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Omm1.3 M1r1[WFxd3C2b4Ppd{BCe3OjeR?= Mn3YOVAxKG6P NXvzRphwPDhiaB?= M17DT2ROW09? M1Xmeolv\HWlZYOgZZBweHSxc3nz NFXJOYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
92.1 NXT0ZZZIS2WubDDDfYNt\SCDc4PhfS=> M{nPR|UxOCCwTR?= M4Tn[|I1NzR6L{eyJIg> MX\EUXNQ NWq0N495cW6mdXPld{B1cGViY3XscEBi[2O3bYXsZZRqd25iYYSgd5VjNUdzwrC= NHX3PWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Omm1.3 MlLDR4VtdCCFeXPs[UBCe3OjeR?= NVjuc2JOPTByIH7N MYGyOE81QC95MjDo MoD6SG1UVw>? M2nxbYlv\HWlZYOgeIhmKGOnbHygZYNkfW23bHH0bY9vKGG2IIP1Zk1IOcLi M3nDSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
A549 NF:wcWdHfW6ldHnvckBCe3OjeR?= NV7zXJpQOTByL{SwNE8yODByIH7N MYeyOEBp NGPMS5B2eHKnZ4XsZZRmeyCjbnSgZYN1cX[jdHXzJHNKWlRz MmHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{MUKxPVkoRjJ4MkGyNVk6RC:jPh?=
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SU-DHL-10 MkjuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHLVIoyPzJxMkWwM|UxOCCwTR?= M1uyUVIwPyCm M{K3SWROW09? M3XUcolv\HWlZYOgZ4VtdC2leXPs[UBienKnc4SgZZQhe3WkLVexJJdqfGhibXnubY1idCClZXzsJIRm[XSqwrC= NV7RR3NURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNFkzQTVpPkK1NFA6Ojl3PD;hQi=>
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Rosetta2 DE3 NHPIR3dHfW6ldHnvckBie3OjeR?= MkPyT4QhRSByLkCwOlIh|ryP Ml3CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 M1TiSmZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? NIDZSWg{OCCvaX7z NUfCeZBqU2liPTCwMlAxPjZizszN MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 MWLGeY5kfGmxbjDhd5NigQ>? M1f6emtlKD1iMD6wNFY4KM7:TR?= NViwN5p5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NX;5SGJPTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= MmPkN|AhdWmwcx?= NYK1RZFmU2liPTCwMlAxPzZizszN NHLXSmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
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Rosetta2 DE3 MlXNSox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? Mk[5N|AhdWmwcx?= MWXLbUA:KDBwMEGwO{DPxE1? NYHTcpZPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NHvT[WhHfW6ldHnvckBie3OjeR?= MYPL[EA:KDBwMEGxO{DPxE1? NYXiR4s4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
MV4-11 M4\tN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUjqdXZNPzJiaB?= NIj2e4hKSzVyIE2gNE4xOTJizszN MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
Rosetta2 DE3 MVHGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 MnrBN|AhdWmwcx?= M4XE[mtqKD1iMD6wNVIh|ryP M37BfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
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Rosetta2 DE3 NUHUV|RFTnWwY4Tpc44h[XO|YYm= MWDL[EA:KDBwMEGyPEDPxE1? Ml3LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
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Rosetta2 DE3 NYfzWI11TnWwY4Tpc44h[XO|YYm= MXfL[EA:KDBwMEGzOkDPxE1? NWrFO3dwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 MnnpSpVv[3Srb36gZZN{[Xl? NV7kcVZYU2RiPTCwMlAyPDdizszN NGnOdY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
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HT-29 NIn0fHRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MU[xNkBp M{ixfWlEPTBiPTCwMlAzKM7:TR?= NIC4SlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEWzOVA1PSd-Mki1N|UxPDV:L3G+
MV4-11 MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MojYO|IhcA>? NX;ER40zUUN3MDC9JFAvODJ|IN88US=> NV;qbIkyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
MV4-11 NVO5W4FbS3m2b4TvfIlkcXS7IHHzd4F6 M1rablQh\GG7cx?= Mmm0TWM2OCB;IECuNFI1KM7:TR?= Mor5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
MV4-11 M4PVfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4LlOFQh\GG7cx?= MnfDTWM2OCB;IECuNFI1KM7:TR?= Ml3KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 M2DqfWZ2dmO2aX;uJIF{e2G7 M3XOclMxKG2rboO= NYrFbY9[UUN3MDC9JFAvODJ6NzFOwG0> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
NALM16 MnXWR5l1d3SxeHnjbZR6KGG|c3H5 NVryZmN1PSCmYYnz NXrFdJdsTUN3MDC9JFAvODNizszN M4rC[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUewNFI1Lz5{OUG3NFAzPDxxYU6=
BL21(DE3)-R3-pRARE2 NHjCe4dHfW6ldHnvckBie3OjeR?= M3PzV|MxKG2rboO= M4CyfmlEPTBiPTCwMlA{OyEQvF2= NXPCWXNqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPVU4OjNpPkK4NVk2PzJ|PD;hQi=>
BL21(DE3) NXfCXll{TnWwY4Tpc44h[XO|YYm= NITJ[ldM\CB;IECuNFM1KM7:TR?= M2jRdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
Rosetta2 DE3 MXfGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 MW[zNEBucW6| NUjKOHprUUN3MDC9JFAvODN3NzFOwG0> M2ryVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
Rosetta2 DE3 NXnObXFuTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= MVqzNEBucW6| MnnVTWM2OCB;IECuNFQzOiEQvF2= M1fYSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
Rosetta2 DE3 MmPoSox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? MkHGN|AhdWmwcx?= MlviTWM2OCB;IECuNFQ3PyEQvF2= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4M{S4O{c,Ojh2NkO0PFc9N2F-
MOLM13 Mmn3R5l1d3SxeHnjbZR6KGG|c3H5 NIPxU441KGSjeYO= NX;yRXRiUUN3MDC9JFAvODV4IN88US=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4M{S4O{c,Ojh2NkO0PFc9N2F-
MOLM13 M4nu[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2K4TlQh\GG7cx?= NYjWOYVHUUN3MDC9JFAvODV4IN88US=> NVLi[mxyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
HL60 MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MWe3NkBp NFjMdXVKSzVyIE2gNE4xPiEQvF2= NGPxPHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
HL60 M2ryZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWWzJIRigXN? MmqwS2M2OCB;IECuNFYh|ryP NFznU4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[1O|A6QSd-Mkm2OVcxQTl:L3G+
NALM6 NIHaeJNEgXSxdH;4bYNqfHliYYPzZZk> Mnr5OUBl[Xm| MV3FR|UxKD1iMD6wOkDPxE1? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
Raji MnzvSpVv[3Srb36gZZN{[Xl? NUPPO|k2UUN3MDC9JFAvODZizszN Ml:3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
Raji M3[ybGZ2dmO2aX;uJIF{e2G7 Mly3OEBp NInDN5JKSzVyIE2gNE4xPjlizszN MoHQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3OVgoRjJ2OUCwO|U5RC:jPh?=
MM1S MmnYRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MXK3NkBp M1XDPWlEPTBiPTCwMlA3QTFizszN MmnwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3MkW0N|UoRjJ7NUK1OFM2RC:jPh?=
BL21 (DE3)-codon plus-RIL NVfvXYsxTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= MmjNZpkh\my3b4Lld4NmdmOnIHHubZNwfHKxcImgZZN{[Xl? MoX1TWM2OCB;IECuNFch|ryP MkSzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OE[3NVgoRjJ6NUi2O|E5RC:jPh?=
22Rv1 NWPFe2pKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M3\0V|k3KGh? NVjMWnlWUUN3MDC9JFAvODdzIN88US=> NH[1XJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
22Rv1 M{TGVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M1;4[VEzKGh? M4jHVmlEPTBiPTCwMlA4OSEQvF2= Ml7sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
MV4-11 M1riSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWDmTmlYUUN3MDC9JFAvODd{IN88US=> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF7NUeyN{c,OjhzOUW3NlM9N2F-
BL21(DE3)-R3-pRARE2 MVXGeY5kfGmxbjDhd5NigQ>? NEnpTnE{OCCvaX7z MoPPTWM2OCB;IECuNFc4KM7:TR?= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
BL21(DE3)-R3-pRARE2 NHrxTJpHfW6ldHnvckBie3OjeR?= MlLGN|AhdWmwcx?= M3rkdWlEPTBiPTCwMlA4PyEQvF2= NIfCToY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG5OVczOyd-MkixPVU4OjN:L3G+
BL21(DE3)-R3-pRARE2 NXzTPWZtTnWwY4Tpc44h[XO|YYm= NHXnWIw{OCCvaX7z MnvtTWM2OCB;IECuNFc4KM7:TR?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd5NkizOEc,Ojl5N{[4N|Q9N2F-
MV4-11 NVjrW5ZkS3m2b4TvfIlkcXS7IHHzd4F6 NXHvUo9UOjRiaB?= NUjNUVlwT0l3MDC9JFAvODhizszN Mm\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzOUGzOlMoRjJ4MUmxN|Y{RC:jPh?=
MV411 NYH5[I1zSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYPzOJhzPzJiaB?= NF\hVVNKSzVyIE2gNE4xQCEQvF2= NX;r[mxDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkizNVQ2OTNpPkK4N|E1PTF|PD;hQi=>
TY82 NWT6O3B7SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NGqwSVI4OiCq NWfad4xIUUN3MDC9JFAvODhyODFOwG0> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV{NUSzOUc,Ojl3MkW0N|U9N2F-
HL60 MUfGeY5kfGmxbjDhd5NigQ>? MX:yOEBp MoD1TWM2OCB;IECuNFg3KM7:TR?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
697 NHW2NpVEgXSxdH;4bYNqfHliYYPzZZk> Mom5OUBl[Xm| M1fJW2VEPTBiPTCwMlA6KM7:TR?= NEG5eoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
Loucy Ml7DR5l1d3SxeHnjbZR6KGG|c3H5 MX21JIRigXN? MWTFR|UxKD1iMD6wPUDPxE1? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
BL21(DE3) MVvGeY5kfGmxbjDhd5NigQ>? MlPPT4QhRSByLkC5NkDPxE1? NHjRb2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
BL21(DE3)-R3-pRARE2 M{PqZWZ2dmO2aX;uJIF{e2G7 M1\V[2tlKD1iMD6xJO69VQ>? M1LxUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUm1NFA4Lz5{OEW5OVAxPzxxYU6=
HT-29 MmPaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV[3NkBp MkLnTWM2OCB;IECuNVA1KM7:TR?= NVjjbo5wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
MM1S MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXLKNmw4PzJiaB?= M3XvOmlEPTBiPTCwMlExQSEQvF2= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3OUSyPEc,OjV3NUm0Nlg9N2F-
LNCAP cells NVT6ZXc4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MYLJR|UxKD1iMD6xNFk3KM7:TR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
T cells MX;GeY5kfGmxbjDhd5NigQ>? NH:0NZEzPCCq Ml\pTWM2OCB;IECuNVEh|ryP MmXMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh|MUS1NVMoRjJ6M{G0OVE{RC:jPh?=
HL60 Mni1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MkO0O|IhcA>? NGG1eG5KSzVyIE2gNE4yOSEQvF2= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh4OUG5OEc,OjZ6NkmxPVQ9N2F-
BL21(DE3) NIn4RolHfW6ldHnvckBie3OjeR?= NVrwcGNzUUN3MDC9JFAvOTJizszN M{DwSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
BL21(DE3) MY\GeY5kfGmxbjDhd5NigQ>? MofXNk42KGh? MoPsTWM2OCB;IECuNVIh|ryP NH73elY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
H1299 M2fzXWZ2dmO2aX;uJIF{e2G7 MYmyOEBp MX7FR|UxKD1iMD6xOVMh|ryP M2HKRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUS5OVIyLz5{OEm0PVUzOTxxYU6=
HD-MB03 NHTrVI9EgXSxdH;4bYNqfHliYYPzZZk> MoLlOUBl[Xm| NIDOVnlGSzVyIE2gNE4yPiEQvF2= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
LNCAP NU[3bGZoSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Ml;2PVYhcA>? NWnjWFN{UUN3MDC9JFAvOTZizszN M1jV[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
Hs578T M3TmSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MoLENVIhcA>? MkLpTWM2OCB;IECuNVYh|ryP Mn;4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MV4-11 NHTneXhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYOxNkBp NX;LfpFJUUN3MDC9JFAvOTZizszN NFLaNVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
LNCAP NV3leY43SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NE[1VYMyOiCq MXPJR|UxKD1iMD6xOkDPxE1? NUTtWHJlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
C4-2B NEHVSVVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUS5OkBp NGf4Xo1KSzVyIE2gNE4yQSEQvF2= NWThOpdQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
C4-2B M4DWc2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M2XVWVEzKGh? MX3JR|UxKD1iMD6xPUDPxE1? NXizRoFjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
Vero E6 M1nZUGFvfGm4aYLhcEBi[3Srdnn0fUBie3OjeR?= MX:0PEBp M4n5[2lEPTBiPTCwMlE6Ojd3IN88US=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OjN3M{i1PUc,OzJ|NUO4OVk9N2F-
MCF7 MoPwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NH;xN4syOiCq MUHJR|UxKD1iMD6yJO69VQ>? M1;scVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
MV4-11 NFnRPFJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MoTKTWM2OCB;IECuNlQh|ryP NYX6N44yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexOFI4PTFpPkK3NVQzPzVzPD;hQi=>
MV4-11 M4DofmN6fG:2b4jpZ4l1gSCjc4PhfS=> M{jK[lczKGh? MoLoTWM2OCB;IECuNlQzKM7:TR?= NYT1ZZVRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NVcxOTFpPkKzOVE4ODFzPD;hQi=>
MX1 NYjOb2RYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NUfkT5dFPzJiaB?= NV7UblRMTUN3MDC9JFAvOjV2IN88US=> NHHyOmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0PVUzOSd-Mki5OFk2OjF:L3G+
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MV4-11 MWLDfZRwfG:6aXPpeJkh[XO|YYm= NIrlUYgzPCCq MXHJR|UxKD1iNj60JO69VQ>? M{\iPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Mk[2PVk6Lz5{N{K2Olk6QTxxYU6=
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Assay
Methods Test Index PMID
Western blot pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax ; c-Myc ; p27 26119999 23792448
Growth inhibition assay Cell viability 23792448
Immunofluorescence GM130 ; MHC / EdU 29074567 28733670
In vivo (+)-JQ1 (50 mg/kg) inhibits tumors growth in mice with NMC 797 xenografts. (+)-JQ1 (50 mg/kg) results in effacement of NUT nuclear speckles in mice with NMC 797 xenografts, consistent with competitive binding to nuclear chromatin. (+)-JQ1 (50 mg/kg) induces strong (grade 31) keratin expression in NMC 797 xenografts. (+)-JQ1 (50 mg/kg) promotes differentiation, tumor regression and prolonged survival in mice models of NMC xenografts. [1] (+)-JQ1 (50 mg/kg) results in a significant prolongation in overall survival of SCID-beige mice orthotopically xenografted after intravenous injection with MM.1S-luc+ cells compared to vehicle-treated animals. [2] (+)-JQ1 (50 mg/kg i.p.) leads to a highly significant increase in survival of mice bearing Raji xenografts. [3]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: MC 11060 cells
  • Concentrations: ~500 nM
  • Incubation Time: 48 hours
  • Method:

    Cells are seeded into white, 384-well microtiter plates at 500 cells per well in a total volume of 50 μL media. The 797, TT and TE10 cells are grown in DMEM containing 1% penicillin/streptomycin and 10% FBS. The Per403 cells are grown in DMEM containing 1 % penicillin/streptomycin and 20% FBS. Patient-derived NMC 11060 cells are grown in RPMI with 10% FBS and 1% penicillin/streptomycin. (+)-JQ1 is delivered to microtiter assay plates by robotic pin transfer. Following a 48 hours incubation at 37℃, cells are lysed and wells are assessed for total ATP content using a commercial proliferation assay. Replicate measurements are analyzed with respect to dose and estimates of IC50 are calculated by logistic regression (GraphPad Prism).

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: Mice bearing NMC 797 xenografts
  • Dosages: 50 mg/kg
  • Administration: intraperitoneal injection
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 91 mg/mL warmed
(199.12 mM)
Water Insoluble
Ethanol '91 mg/mL

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 456.99
Formula

C23H25ClN4O2S

CAS No. 1268524-70-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(SC2=C1C(=NC(C3=NN=C(N32)C)CC(=O)OC(C)(C)C)C4=CC=C(C=C4)Cl)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
How can I reconstitute the compound for in vivo injection?

Answer:
JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

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