(+)-JQ1

For research use only.

Catalog No.S7110

281 publications

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.

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Selleck's (+)-JQ1 has been cited by 281 publications

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Biological Activity

Description (+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.
Features (+)-JQ1 is more effective than (-)-JQ1.
Targets
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
In vitro

(+)-JQ1 enantiomer binds directly into the Kac binding site of BET bromodomains. (+)-JQ1 (500 nM) binds BRD4 competitively with chromatin resulting in differentiation and growth arrest of NMC cells. (+)-JQ1 (500 nM) attenuates rapid proliferation of NMC 797 and Per403 cell lines as demonstrated by reduced Ki67 staining. (+)-JQ1 (500 nM) potently decreases expression of both BRD4 target genes in NMC 797 cells. (+)-JQ1 inhibits cellular viability with IC50 of 4 nM in NMC 11060 cells. [1] (+)-JQ1 results in robust inhibition of MYC expression in MM cell lines. (+)-JQ1 inhibits proliferating of KMS-34 and LR5 with IC50 of 68 nM and 98 nM, respectively. (+)-JQ1 (500 nM)-treated MM.1S cells results in a pronounced decrease in the proportion of cells in S-phase, with a concomitant increase in cells arrested in G0/G1. (+)-JQ1 (500 nM) results in pronounced cellular senescence by beta-galactosidase staining. (+)-JQ1 (800 nM) exposure leads to a significant reduction in cell viability among the majority of CD138+ patient-derived MM samples tested. [2] (+)-JQ1 inhibits growth of LP-1 cells with GI50 of 98 nM. (+)-JQ1 (625 nM) results in an increase in the percentage of LP-1 cells in G0/G1. (+)-JQ1 (500 nM) suppresses the expression of MYC, BRD4 and CDK9 in LP-1 cells. [3] (+)-JQ1 (1 μM) activates HIV transcription in latently infected Jurkat T cells. (+)-JQ1 (50 μM) stimulates predominantly Tat-dependent HIV transcription in both Jurkat and HeLa cells. (+)-JQ1 (5 μM) induces Brd4 dissociation enables Tat to recruit SEC to HIV promoter and induce Pol II CTD phosphorylation and viral transcription in J-Lat A2 cells. JQ1 enables Tat to increase CDK9 T-loop phosphorylation and partially dissociates P-TEFb from 7SK snRNP in Jurkat T cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  M2niTWNmdGxiVnnhZoltcXS7IFHzd4F6 MWiyOVAwPTByL{GwNFAhdk1? NHfLWIMzPC92OD:3NkBp MWPEUXNQ M1fwRolvcGmkaYTzJINmdGxidnnhZoltcXS7IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
BCPAP NIrZUYhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NVPJO5VwOjVyL{WwNE8yODByIH7N NYrzT3p1OjRxNEivO|IhcA>? M4rDbWROW09? MUnpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy NFrrZWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewO|g5OSd-Mk[3NFc5QDF:L3G+
K1  NUHqbZQ4S2WubDDDfYNt\SCDc4PhfS=> MV2yOVAwPTByL{GwNFAhdk1? MXe3NkBp MmLhSG1UVw>? NV3mWW5{[XK{ZYP0d{Bk\WyuIHP5Z4xmKGG2IFewM2cyKHCqYYPl NGjxOGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewO|g5OSd-Mk[3NFc5QDF:L3G+
BCPAP M3fIW2NmdGxiQ4njcIUhSXO|YYm= MV6yOVAwPTByL{GwNFAhdk1? M3G4bFczKGh? NYTOfHhKTE2VTx?= NHq5RoRienKnc4TzJINmdGxiY4njcIUh[XRiR{CvS|EheGijc3W= NVnQNldkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFc5QDFpPkK2O|A4QDhzPD;hQi=>
Hep3B M3TJeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXKwMVExKM7:TR?= M1rIbFUh\A>? Mmi5SG1UVw>? M2P1TGlEPTB;MD6wPEDPxE1? M4TWXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HCCLM3 Mn7RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHufllNOC1zMDFOwG0> Mmq2OUBl MWPEUXNQ NIDXOohKSzVyPUCuNVQh|ryP M2TLT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HuH7 NWnDW3hZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TQN|AuOTBizszN MWO1JIQ> NGe0dJdFVVOR MVXJR|UxRTBwMkGg{txO M3\STFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HepG2 MlPIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fFPFAuOTBizszN MmX3OUBl NHThS4dFVVOR MYLJR|UxRTBwM{Sg{txO MlziQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
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MHCC97H NVTWVVQ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFKxXpUxNTFyIN88US=> M3;KOlUh\A>? MmeySG1UVw>? M4POT2lEPTB;MD60NUDPxE1? NWTo[JJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
Hep3B MV\D[YxtKEO7Y3zlJGF{e2G7 NI\KS5cxNjFxMD61M|IvPSEQvF2= MkW1OFghcA>? MXfEUXNQ M3fSSIxm[WS|IITvJIEhe3Wkc4ThcpRq[WxiYXPjeY12dGG2aX;uJI9nKEiFQzDj[YxteyCrbjDzeYIuTzFicHjhd4XDqA>? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HCCLM3 Mor4R4VtdCCFeXPs[UBCe3OjeR?= MnnnNE4yNzBwNT:yMlUh|ryP Ml\sOFghcA>? MVjEUXNQ M17ZWoxm[WS|IITvJIEhe3Wkc4ThcpRq[WxiYXPjeY12dGG2aX;uJI9nKEiFQzDj[YxteyCrbjDzeYIuTzFicHjhd4XDqA>? NV3qdFduRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
Hep3B MYfBdI9xfG:|aYOgRZN{[Xl? NUW5NodTOC5zL{CuOU8zNjVizszN MkPSOFghcA>? MVXEUXNQ M4ewVoFkfGm4YYTld{Bk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOTDlfJBz\XO|aX;uJIFv\CCrbnT1Z4VlKFCDUmCgZ4xm[X[jZ3WgZZMhf2WubDDhd{BkgXSxY3jyc41mKGNicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21iZoLvcUBucXSxY3jvcoRzcWF? MlXHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HCCLM3 NETZfYZCeG:ydH;zbZMhSXO|YYm= MWCwMlEwOC53L{KuOUDPxE1? MUG0PEBp NEjFT|JFVVOR MoL2ZYN1cX[jdHXzJINie3Cjc3WtN{BidmRiY3HzdIF{\S17IHX4dJJme3Orb36gZY5lKGmwZIXj[YQhWEGUUDDjcIVifmGpZTDhd{B4\WyuIHHzJIN6fG:laILvcYUh[yC{ZXzlZZNmKGmwdH:geIhmKGO7dH;wcIF{dSCocn;tJI1qfG:laH;u[JJq[Q>? NU\kbodERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
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A549 MWTGeY5kfGmxbjDBd5NigQ>? M1j2bVEwOi53L{Wg{txO MkmwNVIhcA>? MlPTxsB4\WGtbImg[IVkemWjc3XkJGJkdC1{IHzleoVtew>? NFK0XGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
H1299 NVPyXmpiTnWwY4Tpc44hSXO|YYm= M1jxeFEwOi53L{Wg{txO NFjScJUyOiCq NI\6NVbDqHenYXvsfUBl\WO{ZXHz[YQhSmOuLUKgcIV3\Wy| NWflOXplRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H157 NU\HepR[TnWwY4Tpc44hSXO|YYm= MXqxM|IvPS93IN88US=> MoDyNVIhcA>? NGT3PINl\WO{ZXHz[YQhTFJ2IHX4dJJme3Orb36= M3PM[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
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C8161 NXztXm04S2WubDDWbYFjcWyrdImgRZN{[Xl? MWGwMVIh|ryP MWK0JIQ> MYXEUXNQ M17HNYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M4jY[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
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Mel202 MnXzR4VtdCCYaXHibYxqfHliQYPzZZk> MmrWNE0zKM7:TR?= M2PUelQh\A>? Ml24SG1UVw>? M1GxXYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MnTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel270 Mn:3R4VtdCCYaXHibYxqfHliQYPzZZk> MUSwMVIh|ryP MkK5OEBl M2XUbWROW09? MWfk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NV;rWIFRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1 NHHpWVhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NFi2cHExNTJizszN NI\tbnE1KGR? M2q4b2ROW09? M3PaXoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M{LVT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
92.1 NXe1UWdMSXCxcITvd4l{KEG|c3H5 NV;UeGt2PTByIH7N MoC4OFghcA>? NEHHXINFVVOR NFXJeYRqdmS3Y3XzJIFxd3C2b4Ppdy=> NV3ncHZlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1.3 NUfN[2JJSXCxcITvd4l{KEG|c3H5 NHnpXo82ODBibl2= NEDwTpU1QCCq M1;pZ2ROW09? MoTlbY5lfWOnczDhdI9xfG:|aYO= NF\NUlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
92.1 MojtR4VtdCCFeXPs[UBCe3OjeR?= NH7LO242ODBibl2= NHn1TpMzPC92OD:3NkBp NFToN5FFVVOR NGfCVZdqdmS3Y3XzJJRp\SClZXzsJIFk[3WvdXzheIlwdiCjdDDzeYIuTzIEoB?= NFHFWnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Omm1.3 NV;iZYliS2WubDDDfYNt\SCDc4PhfS=> NFPzemM2ODBibl2= NEPEVFIzPC92OD:3NkBp MWLEUXNQ NV\UfHZ4cW6mdXPld{B1cGViY3XscEBi[2O3bYXsZZRqd25iYYSgd5VjNUdzwrC= NYrYSVZORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
A549 NWXDdYhUTnWwY4Tpc44hSXO|YYm= M{jRfVExOC92MECvNVAxOCCwTR?= M1j6VVI1KGh? NWO2SHh5fXC{ZXf1cIF1\XNiYX7kJIFkfGm4YYTld{BUUVKWMR?= NGDEOmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkKxNlE6QSd-Mk[yNVIyQTl:L3G+
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Rosetta2 DE3 M2ewUmZ2dmO2aX;uJIF{e2G7 NHPrOmRM\CB;IECuNFA3OiEQvF2= NYjwOZNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
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MV4-11 NXyyT4JCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGnX[3A4OiCq Mn;aTWM2OCB;IECuNFI{KM7:TR?= MnS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NUm0NlgoRjJ3NUW5OFI5RC:jPh?=
MV4-11 MVnDfZRwfG:6aXPpeJkh[XO|YYm= MojjOEBl[Xm| MnmzTWM2OCB;IECuNFI1KM7:TR?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4M{S4O{c,Ojh2NkO0PFc9N2F-
MV4-11 NWDIPJZCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1PEVVQh\GG7cx?= NFfaT3VKSzVyIE2gNE4xOjRizszN MoDOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 NYm0eVlDTnWwY4Tpc44h[XO|YYm= NITRO3o{OCCvaX7z Mm[2TWM2OCB;IECuNFI5PyEQvF2= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
NALM16 NYWzUFJkS3m2b4TvfIlkcXS7IHHzd4F6 NILCN2Q2KGSjeYO= NXPQNYdiTUN3MDC9JFAvODNizszN MlrvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
BL21(DE3)-R3-pRARE2 NImyW4VHfW6ldHnvckBie3OjeR?= MVyzNEBucW6| MleyTWM2OCB;IECuNFM{KM7:TR?= NV;GWHZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPVU4OjNpPkK4NVk2PzJ|PD;hQi=>
BL21(DE3) NWPYfVFSTnWwY4Tpc44h[XO|YYm= NXXPRpFNU2RiPTCwMlA{PCEQvF2= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
Rosetta2 DE3 NXOxZYE1Tmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= MnzsN|AhdWmwcx?= MUHJR|UxKD1iMD6wN|U4KM7:TR?= NYP4T2JYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NELme2ZHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 M33u[|MxKG2rboO= MVnJR|UxKD1iMD6wOFIzKM7:TR?= M4PJXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
Rosetta2 DE3 MlrrSox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? NVzwdGdnOzBibXnudy=> NVTKclBUUUN3MDC9JFAvODR4NzFOwG0> M2TYbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
MOLM13 NXzURXpJS3m2b4TvfIlkcXS7IHHzd4F6 M4fUZlQh\GG7cx?= MUnJR|UxKD1iMD6wOVYh|ryP MnfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
MOLM13 Ml3US5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmDOOEBl[Xm| NHL2OoJKSzVyIE2gNE4xPTZizszN NHi4W449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
HL60 NI\kN2pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MU[3NkBp MojwTWM2OCB;IECuNFYh|ryP NXXu[XVrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxO|AxOjRpPkK5NVcxODJ2PD;hQi=>
HL60 M3PnU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NW\LVFFCOyCmYYnz NX7qcFQ2T0N3MDC9JFAvODZizszN M4nxb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkW3NFk6Lz5{OU[1O|A6QTxxYU6=
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Raji NWXYbnA4TnWwY4Tpc44h[XO|YYm= MULJR|UxKD1iMD6wOkDPxE1? MofxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
Raji MWHGeY5kfGmxbjDhd5NigQ>? NFjVdo41KGh? MXTJR|UxKD1iMD6wOlkh|ryP NXL6V5VHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4PThpPkK0PVAxPzV6PD;hQi=>
MM1S NXPG[Fl[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NWLXVnNTPzJiaB?= MmC5TWM2OCB;IECuNFY6OSEQvF2= MmjZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3MkW0N|UoRjJ7NUK1OFM2RC:jPh?=
BL21 (DE3)-codon plus-RIL NXHEdXJJTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= M{\DU4J6KG[udX;y[ZNk\W6lZTDhcol{d3S{b4D5JIF{e2G7 Mly2TWM2OCB;IECuNFch|ryP NY\UbFA1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1PFY4OThpPkK4OVg3PzF6PD;hQi=>
22Rv1 MlHtRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MoHxPVYhcA>? MYHJR|UxKD1iMD6wO|Eh|ryP MnHwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
22Rv1 Mo[5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M2\BPVEzKGh? NWnWT2tYUUN3MDC9JFAvODdzIN88US=> NWTYTo4{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
MV4-11 MmTGRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MmHoTWM2OCB;IECuNFczKM7:TR?= NIDLOWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG5OVczOyd-MkixPVU4OjN:L3G+
BL21(DE3)-R3-pRARE2 NVT5TXFxTnWwY4Tpc44h[XO|YYm= NXX2XG8yOzBibXnudy=> M37oNWlEPTBiPTCwMlA4PyEQvF2= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
BL21(DE3)-R3-pRARE2 Mmf4SpVv[3Srb36gZZN{[Xl? M1nHNVMxKG2rboO= MkDqTWM2OCB;IECuNFc4KM7:TR?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF7NUeyN{c,OjhzOUW3NlM9N2F-
BL21(DE3)-R3-pRARE2 NEHUXZpHfW6ldHnvckBie3OjeR?= NIfIfJQ{OCCvaX7z MULJR|UxKD1iMD6wO|ch|ryP NYW3cmZJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3O|Y5OzRpPkK5O|c3QDN2PD;hQi=>
MV4-11 MoLKR5l1d3SxeHnjbZR6KGG|c3H5 NYjxdWJMOjRiaB?= NHq0VG9IUTVyIE2gNE4xQCEQvF2= NVHxN3ZTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVE{PjNpPkK2NVkyOzZ|PD;hQi=>
MV411 M4XkZWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3PWeFczKGh? NUDIUYc3UUN3MDC9JFAvODhizszN NGnMUJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
TY82 NFPqXZJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NHnINYM4OiCq MUPJR|UxKD1iMD6wPFA5KM7:TR?= NXHaWpp3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NlU1OzVpPkK5OVI2PDN3PD;hQi=>
HL60 M1zKcmZ2dmO2aX;uJIF{e2G7 NY\oXFNWOjRiaB?= NGfjVGhKSzVyIE2gNE4xQDZizszN NWXvd2NCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
697 Mn;tR5l1d3SxeHnjbZR6KGG|c3H5 NUHBWW5xPSCmYYnz MUnFR|UxKD1iMD6wPUDPxE1? MmfrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
Loucy MYXDfZRwfG:6aXPpeJkh[XO|YYm= MmLNOUBl[Xm| MoLCSWM2OCB;IECuNFkh|ryP M{jxVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUewNFI1Lz5{OUG3NFAzPDxxYU6=
BL21(DE3) NFjYTo5HfW6ldHnvckBie3OjeR?= MXjL[EA:KDBwMEmyJO69VQ>? M132S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
BL21(DE3)-R3-pRARE2 NYThdFMxTnWwY4Tpc44h[XO|YYm= MnHLT4QhRSByLkGg{txO MlS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OUWwNFcoRjJ6NUm1NFA4RC:jPh?=
HT-29 NWjNUno5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkPvO|IhcA>? MWPJR|UxKD1iMD6xNFQh|ryP Mmm3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NUm0NlgoRjJ3NUW5OFI5RC:jPh?=
MM1S MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1vXRlczKGh? M4TsbWlEPTBiPTCwMlExQSEQvF2= NELXbJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1PVQzQCd-MkW1OVk1Ojh:L3G+
LNCAP cells M2HqcmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M2fpcGlEPTBiPTCwMlExQTZizszN M2LuZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
T cells MmXuSpVv[3Srb36gZZN{[Xl? MXeyOEBp NWDncItGUUN3MDC9JFAvOTFizszN MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDNzNEWxN{c,Ojh|MUS1NVM9N2F-
HL60 MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NGDKe4M4OiCq NYXJTIU5UUN3MDC9JFAvOTFizszN M4nwOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OE[5NVk1Lz5{Nki2PVE6PDxxYU6=
BL21(DE3) MmrCSpVv[3Srb36gZZN{[Xl? M163WWlEPTBiPTCwMlEzKM7:TR?= MnT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
BL21(DE3) MoXjSpVv[3Srb36gZZN{[Xl? NYq1XIJmOi53IHi= NIHoUnhKSzVyIE2gNE4yOiEQvF2= NFPSbY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
H1299 MmTSSpVv[3Srb36gZZN{[Xl? MXqyOEBp NUjh[Zd5TUN3MDC9JFAvOTV|IN88US=> NF3aUmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0PVUzOSd-Mki5OFk2OjF:L3G+
HD-MB03 NVPEXXh2S3m2b4TvfIlkcXS7IHHzd4F6 MljjOUBl[Xm| NXrsXpJyTUN3MDC9JFAvOTZizszN MnTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
LNCAP MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NVPke2NJQTZiaB?= NWf1coRsUUN3MDC9JFAvOTZizszN M{HFOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
Hs578T NEDp[3NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NWroPWdOOTJiaB?= NV\Zblh{UUN3MDC9JFAvOTZizszN NGjkSWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
MV4-11 Moe1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NFXQZlYyOiCq M131fmlEPTBiPTCwMlE3KM7:TR?= MoTDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
LNCAP NHn2XppCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MmTxNVIhcA>? MWfJR|UxKD1iMD6xOkDPxE1? M33CUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
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C4-2B NX7zV|k3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{fCTFEzKGh? M3vEXGlEPTBiPTCwMlE6KM7:TR?= NHe3b2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
Vero E6 NXf3dGw{SW62aY\pdoFtKGGldHn2bZR6KGG|c3H5 NEnW[XE1QCCq MWDJR|UxKD1iMD6xPVI4PSEQvF2= MlHoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzJ|NUO4OVkoRjN{M{WzPFU6RC:jPh?=
MCF7 NFvleXFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M2f6blEzKGh? NFXRN2xKSzVyIE2gNE4zKM7:TR?= NWfBUW5QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MV4-11 M4jrXGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MlLTTWM2OCB;IECuNlQh|ryP M2DkcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUSyO|UyLz5{N{G0Nlc2OTxxYU6=
MV4-11 MUXDfZRwfG:6aXPpeJkh[XO|YYm= NYfYcGdIPzJiaB?= Mn60TWM2OCB;IECuNlQzKM7:TR?= NVLk[HY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NVcxOTFpPkKzOVE4ODFzPD;hQi=>
MX1 MmPqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MX:3NkBp M13HR2VEPTBiPTCwMlI2PCEQvF2= M4XMUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUS5OVIyLz5{OEm0PVUzOTxxYU6=
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HFL1 NIPaO2lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUSxNkBp MVTJR|UxKD1iMD6yPUDPxE1? NUOycW52RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MDA-MB-231 M{nyXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVWzJIRigXN? NEDwfXNISzVyIE2gNE4{KM7:TR?= NX3NUXVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
MV4-11 NH72U29CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MVu0PEBp MYrFR|UxKD1iMD6zN|EyOyEQvF2= NF6wd2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW5OVAxPyd-Mki1PVUxODd:L3G+
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HL60 NEm5fYNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlfkOFghcA>? NEX0OZNGSzVyIE2gNE44PDF|MTFOwG0> NWTHWIRVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1PVUxODdpPkK4OVk2ODB5PD;hQi=>
NCI-H1975 MlPrRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NHn5SpAyOiCq NFizW3RKSzVyIE2gNU4zOyEQvF2= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
SAE M1HhUmZ2dmO2aX;uJIF{e2G7 M{P5OFQhcA>? NIqzZ2JKSzVyIE2gNU4{QCEQvF2= NYK3dHNqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
SAE MlzUSpVv[3Srb36gZZN{[Xl? NH3DZYU1KGh? M3zIZWlEPTBiPTCxMlQ6KM7:TR?= Mm\EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4NEm3OFEoRjJ7NkS5O|QyRC:jPh?=
SAE NWTZfVJyTnWwY4Tpc44h[XO|YYm= MojpOEBp MXnJR|UxKD1iMT61NUDPxE1? NEiy[HI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0PVc1OSd-Mkm2OFk4PDF:L3G+
U2OS MVPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MYqxNkBp NIDreFRKSzVyIE2gNU43OiEQvF2= Mn\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
SAE M3;kXWZ2dmO2aX;uJIF{e2G7 MYi0JIg> MXjJR|UxKD1iMT62N{DPxE1? NFjDWHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0PVc1OSd-Mkm2OFk4PDF:L3G+
A549 MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlrvNVIhcA>? NIrscIVKSzVyIE2gNU43PyEQvF2= NGS2NpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
MCF7 NUHaN5dYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2XXUFMh\GG7cx?= M{fzeWdEPTBiPTCxMlch|ryP NF3Udm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+
DU145 NXf5cGV{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MlPsPVYhcA>? MlTzTWM2OCB;IEKuOVIh|ryP MlrBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
DU145 MVPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NGXHb3oyOiCq NEXydm1KSzVyIE2gNk42OiEQvF2= NH\xSok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
T47D M1XJOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUmzJIRigXN? Mn;5S2M2OCB;IEKuPEDPxE1? M4XlblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
PC3 NYjrUHdFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NG[3O|M6PiCq MnLXTWM2OCB;IEOuNFEh|ryP MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
PC3 M2jGc2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3HMeFEzKGh? M{XqOWlEPTBiPTCzMlAyKM7:TR?= MmW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
HeLa MmL6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MlLuNVIhcA>? NYTONVNQUUN3MDC9JFMvPzZizszN M4fWbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
K562 MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmTEN{Bl[Xm| NV\Kc2F1T0N3MDC9JFMvQCEQvF2= NXvtenJSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
A2780 NF3wTo1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml\qN{Bl[Xm| NWrF[nF[T0N3MDC9JFQh|ryP MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
HL60 M2nNPWN6fG:2b4jpZ4l1gSCjc4PhfS=> NV;HWI1OOjRiaB?= NHLqTFVKSzVyIE2gOU4{KM7:TR?= Ml22QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{Nk[5PVkoRjJ5Mk[2PVk6RC:jPh?=
MV4-11 MVfDfZRwfG:6aXPpeJkh[XO|YYm= MYiyOEBp MV3JR|UxKD1iNj60JO69VQ>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJ4Nkm5PUc,Ojd{Nk[5PVk9N2F-
K562 M4LocWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MXS3NkBp NHXUbJpKSzVyIE2gPU4yOiEQvF2= NUDLcIVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkizNVQ2OTNpPkK4N|E1PTF|PD;hQi=>
OVCAR5 NVTnfFZTT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlfrN{Bl[Xm| NGrYTYJISzVyIE2gNVIh|ryP NVW3bmRrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
In vivo (+)-JQ1 (50 mg/kg) inhibits tumors growth in mice with NMC 797 xenografts. (+)-JQ1 (50 mg/kg) results in effacement of NUT nuclear speckles in mice with NMC 797 xenografts, consistent with competitive binding to nuclear chromatin. (+)-JQ1 (50 mg/kg) induces strong (grade 31) keratin expression in NMC 797 xenografts. (+)-JQ1 (50 mg/kg) promotes differentiation, tumor regression and prolonged survival in mice models of NMC xenografts. [1] (+)-JQ1 (50 mg/kg) results in a significant prolongation in overall survival of SCID-beige mice orthotopically xenografted after intravenous injection with MM.1S-luc+ cells compared to vehicle-treated animals. [2] (+)-JQ1 (50 mg/kg i.p.) leads to a highly significant increase in survival of mice bearing Raji xenografts. [3]

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: MC 11060 cells
  • Concentrations: ~500 nM
  • Incubation Time: 48 hours
  • Method:

    Cells are seeded into white, 384-well microtiter plates at 500 cells per well in a total volume of 50 μL media. The 797, TT and TE10 cells are grown in DMEM containing 1% penicillin/streptomycin and 10% FBS. The Per403 cells are grown in DMEM containing 1 % penicillin/streptomycin and 20% FBS. Patient-derived NMC 11060 cells are grown in RPMI with 10% FBS and 1% penicillin/streptomycin. (+)-JQ1 is delivered to microtiter assay plates by robotic pin transfer. Following a 48 hours incubation at 37℃, cells are lysed and wells are assessed for total ATP content using a commercial proliferation assay. Replicate measurements are analyzed with respect to dose and estimates of IC50 are calculated by logistic regression (GraphPad Prism).


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: Mice bearing NMC 797 xenografts
  • Dosages: 50 mg/kg
  • Administration: intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 456.99
Formula

C23H25ClN4O2S

CAS No. 1268524-70-4
Storage powder
in solvent
Synonyms N/A
Smiles CC1=C(SC2=C1C(=NC(C3=NN=C(N32)C)CC(=O)OC(C)(C)C)C4=CC=C(C=C4)Cl)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    How can I reconstitute the compound for in vivo injection?

  • Answer:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID