(+)-JQ1

For research use only.

Catalog No.S7110

271 publications

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.

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Selleck's (+)-JQ1 has been cited by 271 publications

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Biological Activity

Description (+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.
Features (+)-JQ1 is more effective than (-)-JQ1.
Targets
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
In vitro

(+)-JQ1 enantiomer binds directly into the Kac binding site of BET bromodomains. (+)-JQ1 (500 nM) binds BRD4 competitively with chromatin resulting in differentiation and growth arrest of NMC cells. (+)-JQ1 (500 nM) attenuates rapid proliferation of NMC 797 and Per403 cell lines as demonstrated by reduced Ki67 staining. (+)-JQ1 (500 nM) potently decreases expression of both BRD4 target genes in NMC 797 cells. (+)-JQ1 inhibits cellular viability with IC50 of 4 nM in NMC 11060 cells. [1] (+)-JQ1 results in robust inhibition of MYC expression in MM cell lines. (+)-JQ1 inhibits proliferating of KMS-34 and LR5 with IC50 of 68 nM and 98 nM, respectively. (+)-JQ1 (500 nM)-treated MM.1S cells results in a pronounced decrease in the proportion of cells in S-phase, with a concomitant increase in cells arrested in G0/G1. (+)-JQ1 (500 nM) results in pronounced cellular senescence by beta-galactosidase staining. (+)-JQ1 (800 nM) exposure leads to a significant reduction in cell viability among the majority of CD138+ patient-derived MM samples tested. [2] (+)-JQ1 inhibits growth of LP-1 cells with GI50 of 98 nM. (+)-JQ1 (625 nM) results in an increase in the percentage of LP-1 cells in G0/G1. (+)-JQ1 (500 nM) suppresses the expression of MYC, BRD4 and CDK9 in LP-1 cells. [3] (+)-JQ1 (1 μM) activates HIV transcription in latently infected Jurkat T cells. (+)-JQ1 (50 μM) stimulates predominantly Tat-dependent HIV transcription in both Jurkat and HeLa cells. (+)-JQ1 (5 μM) induces Brd4 dissociation enables Tat to recruit SEC to HIV promoter and induce Pol II CTD phosphorylation and viral transcription in J-Lat A2 cells. JQ1 enables Tat to increase CDK9 T-loop phosphorylation and partially dissociates P-TEFb from 7SK snRNP in Jurkat T cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  Mmr0R4VtdCCYaXHibYxqfHliQYPzZZk> M1T2[VI2OC93MECvNVAxOCCwTR?= NX76NmQ3OjRxNEivO|IhcA>? M1zBPGROW09? M1zZW4lvcGmkaYTzJINmdGxidnnhZoltcXS7IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI> M33XSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP MkLaR4VtdCCYaXHibYxqfHliQYPzZZk> NXnlWG5ZOjVyL{WwNE8yODByIH7N MojSNlQwPDhxN{KgbC=> NGHlUZJFVVOR NFXRcYhqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIHTvd4UuKGGwZDD0bY1mNSCmZYDlcoRmdnRibXHucoVz M13yeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
K1  MWnD[YxtKEO7Y3zlJGF{e2G7 NF\GS2czPTBxNUCwM|ExODBibl2= MYe3NkBp M1nYXmROW09? NFj0TGhienKnc4TzJINmdGxiY4njcIUh[XRiR{CvS|EheGijc3W= M37WRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP M2nFVWNmdGxiQ4njcIUhSXO|YYm= NIK3Z4EzPTBxNUCwM|ExODBibl2= MX:3NkBp MYrEUXNQ NFO2XFBienKnc4TzJINmdGxiY4njcIUh[XRiR{CvS|EheGijc3W= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
Hep3B NWnsbIdqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\G[FkxNTFyIN88US=> MYC1JIQ> MVTEUXNQ MX;JR|UxRTBwMEig{txO MoXkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HCCLM3 NFXCNXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zzWFAuOTBizszN MkP4OUBl MXTEUXNQ M1vIbWlEPTB;MD6xOEDPxE1? NFm1XI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HuH7 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\xXnZFOC1zMDFOwG0> M3vqb|Uh\A>? MlnlSG1UVw>? MlnQTWM2OD1yLkKxJO69VQ>? NVH0b|hERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HepG2 NFeyZmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rGUlAuOTBizszN Ml7zOUBl M2W0[WROW09? MlzlTWM2OD1yLkO0JO69VQ>? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
SMMC7721 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7wNE0yOCEQvF2= MXW1JIQ> M1jZUWROW09? MlHwTWM2OD1yLkSxJO69VQ>? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
BEL7402 M3\iPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\rVVAuOTBizszN NHXSTFE2KGR? Ml7iSG1UVw>? MUnJR|UxRTBwNEeg{txO NHTZNHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
MHCC97H NGTQb2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXCNE0yOCEQvF2= M1S1WVUh\A>? M3zVRmROW09? MmHOTWM2OD1yLkSxJO69VQ>? MlfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
Hep3B NF\rWIxE\WyuIFP5Z4xmKEG|c3H5 MorXNE4yNzBwNT:yMlUh|ryP MXG0PEBp MnTnSG1UVw>? NWrpVHlkdGWjZIOgeI8h[SC|dXLzeIFvfGmjbDDhZ4N2dXWuYYTpc44hd2ZiSFPDJINmdGy|IHnuJJN2[i2JMTDwbIF{\cLi M{DYc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HCCLM3 Ml\jR4VtdCCFeXPs[UBCe3OjeR?= MkftNE4yNzBwNT:yMlUh|ryP NX;FPZREPDhiaB?= MYTEUXNQ MV\s[YFleyC2bzDhJJN2[nO2YX70bYFtKGGlY4XteYxifGmxbjDv[kBJS0NiY3XscJMhcW5ic4XiMWcyKHCqYYPlxsA> M4DBXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
Hep3B NIqxXmhCeG:ydH;zbZMhSXO|YYm= M4K1[VAvOS9yLkWvNk42KM7:TR?= NWjjNZdNPDhiaB?= MmLNSG1UVw>? MorhZYN1cX[jdHXzJINie3Cjc3WtN{BidmRiY3HzdIF{\S17IHX4dJJme3Orb36gZY5lKGmwZIXj[YQhWEGUUDDjcIVifmGpZTDhd{B4\WyuIHHzJIN6fG:laILvcYUh[yC{ZXzlZZNmKGmwdH:geIhmKGO7dH;wcIF{dSCocn;tJI1qfG:laH;u[JJq[Q>? M2fXPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HCCLM3 NYC0dJVzSXCxcITvd4l{KEG|c3H5 M4[xWVAvOS9yLkWvNk42KM7:TR?= MkHYOFghcA>? MVfEUXNQ NVXwNm5E[WO2aY\heIV{KGOjc4Dhd4UuOyCjbnSgZ4F{eGG|ZT25JIV5eHKnc4Ppc44h[W6mIHnu[JVk\WRiUFHSVEBkdGWjdnHn[UBieyC5ZXzsJIF{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnueI8hfGinIHP5eI9xdGG|bTDmdo9uKG2rdH;jbI9v\HKrYR?= NETLV4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
A549 NED0b2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUiwMlEuOTBizszN M4OxcFczKGh? MmfhbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NX\vZppURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H157 NH2xZmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYiwMlEuOTBizszN NIG2XI84OiCq MWHpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NXrE[JE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDpVnYxNjFvMUCg{txO NF;VbFI4OiCq NF7Fe3ZqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NXv6U5NrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
A549 NI\aO|hHfW6ldHnvckBCe3OjeR?= MofBNU8zNjVxNTFOwG0> MYSxNkBp MoLaxsB4\WGtbImg[IVkemWjc3XkJGJkdC1{IHzleoVtew>? M3TOZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H1299 MW\GeY5kfGmxbjDBd5NigQ>? NGfob|kyNzJwNT:1JO69VQ>? MlfINVIhcA>? MXpCpJdm[WuueTDk[YNz\WG|ZXSgRoNtNTJibHX2[Yx{ M{Xxe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H157 NXfBdGVETnWwY4Tpc44hSXO|YYm= NGG5flIyNzJwNT:1JO69VQ>? MmDUNVIhcA>? Mnjv[IVkemWjc3XkJGRTPCCneIDy[ZN{cW:w MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
H1299 MU\GeY5kfGmxbjDBd5NigQ>? MWKxM|IvPS93IN88US=> NV\iOpI{OTJiaB?= Mniy[IVkemWjc3XkJGRTPCCneIDy[ZN{cW:w MmfHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
C8161 NVzoSZdNS2WubDDWbYFjcWyrdImgRZN{[Xl? MoKzNE0zKM7:TR?= Mn\kOEBl Mlr3SG1UVw>? NIW1XG9l\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= Mn:yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel285 Ml3ZR4VtdCCYaXHibYxqfHliQYPzZZk> M1ruT|AuOiEQvF2= MWe0JIQ> M2\nXmROW09? MUHk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NXvLXlBxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Mel290 NX25UWk2S2WubDDWbYFjcWyrdImgRZN{[Xl? MmLPNE0zKM7:TR?= M2\0fVQh\A>? NUXkfZBoTE2VTx?= Ml7y[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NFX2fIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
92.1 NEfIZ5VE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NUPBVJE1OC1{IN88US=> MkHFOEBl NFT2R|BFVVOR NHTQV2Jl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NHjWXYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Omm1.3 MYLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MXOwMVIh|ryP M4XRUVQh\A>? M1\TVWROW09? MVzk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NXv6SZk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Mel202 MYXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NV;WOYlGOC1{IN88US=> MkHwOEBl NV7GfHNmTE2VTx?= Mnq4[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MnOyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel270 Mk[yR4VtdCCYaXHibYxqfHliQYPzZZk> MoLzNE0zKM7:TR?= MWK0JIQ> NWPuSndxTE2VTx?= Mkjl[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NHLacGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Omm1 MUDD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NGjDT4kxNTJizszN M1TkNlQh\A>? M4LVd2ROW09? NV71UHpS\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M2fOTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
92.1 NUn0T2RnSXCxcITvd4l{KEG|c3H5 NGW4WZA2ODBibl2= MVq0PEBp NFPqW3BFVVOR NF;PNIpqdmS3Y3XzJIFxd3C2b4Ppdy=> MnjpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Omm1.3 NX6wXGZzSXCxcITvd4l{KEG|c3H5 MXS1NFAhdk1? NYrweZF4PDhiaB?= MkXySG1UVw>? NHvwZ29qdmS3Y3XzJIFxd3C2b4Ppdy=> Moq2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
92.1 MXzD[YxtKEO7Y3zlJGF{e2G7 NXj2XIhCPTByIH7N MmL5NlQwPDhxN{KgbC=> NEnWc|NFVVOR M322dYlv\HWlZYOgeIhmKGOnbHygZYNkfW23bHH0bY9vKGG2IIP1Zk1IOcLi NV6wUndsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1.3 NGfPdGVE\WyuIFP5Z4xmKEG|c3H5 NFfXRpU2ODBibl2= MWmyOE81QC95MjDo MlTvSG1UVw>? MU\pcoR2[2W|IITo[UBk\WyuIHHjZ5VufWyjdHnvckBifCC|dXKtS|HDqA>? NEHifYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
A549 M4HvUmZ2dmO2aX;uJGF{e2G7 MWCxNFAwPDByL{GwNFAhdk1? NYHRNmw4OjRiaB?= MmrTeZBz\We3bHH0[ZMh[W6mIHHjeIl3[XSnczDTTXJVOQ>? M{jabVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkGyNVk6Lz5{NkKxNlE6QTxxYU6=
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OCI-Ly3 MmDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHn[|gxOTd{L{K1NE82ODBibl2= NWfDb2Q{Oi95IHS= NWrPToVSTE2VTx?= M1;OWYlv\HWlZYOgZ4VtdC2leXPs[UBienKnc4SgZZQhe3WkLVexJJdqfGhibXnubY1idCClZXzsJIRm[XSqwrC= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTByOUK5OUc,OjVyMEmyPVU9N2F-
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SU-DHL-10 NFuwPWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXDbHpvOTd{L{K1NE82ODBibl2= Mkj2Nk84KGR? NYHxd|NETE2VTx?= M{TuSIlv\HWlZYOgZ4VtdC2leXPs[UBienKnc4SgZZQhe3WkLVexJJdqfGhibXnubY1idCClZXzsJIRm[XSqwrC= NGf4cVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwPVI6PSd-MkWwNFkzQTV:L3G+
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Rosetta2 DE3 MmPnSpVv[3Srb36gZZN{[Xl? NF3sd5ZM\CB;IECuNFA3OiEQvF2= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 NVi5UnNFTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= MYezNEBucW6| M1L5O2tqKD1iMD6wNFY3KM7:TR?= MlnzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 NF\qXZFHfW6ldHnvckBie3OjeR?= M4nXPGtlKD1iMD6wNFY4KM7:TR?= Ml64QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 M{jmfWZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? NXrVXnB{OzBibXnudy=> MnnmT4khRSByLkCwO|Yh|ryP NX63WlFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
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Rosetta2 DE3 NIWwPZBHfW6ldHnvckBie3OjeR?= M4foTWtlKD1iMD6wNVE4KM7:TR?= NGjCTWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
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Rosetta2 DE3 Mnu3Sox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? NWnNc29oOzBibXnudy=> NHvQVZpMcSB;IECuNFEzKM7:TR?= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
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Rosetta2 DE3 M4G1cGZ2dmO2aX;uJIF{e2G7 NV7WSlRVU2RiPTCwMlAyOjhizszN NH;Y[GI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
Rosetta2 DE3 MV\GcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 NGflOng{OCCvaX7z M2HOWGtqKD1iMD6wNVMzKM7:TR?= MnTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 NY\SOIE{TnWwY4Tpc44h[XO|YYm= NEDBSWhM\CB;IECuNFE{PiEQvF2= NYK2eItTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NYT1[Hc6TnWwY4Tpc44h[XO|YYm= NHzPbI9M\CB;IECuNFE1PyEQvF2= MoXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 MljESox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? NVrNSXlIOzBibXnudy=> MUnLbUA:KDBwMEG0PUDPxE1? M3XXc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
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MM1S NWX4NJRMS3m2b4TvfIlkcXS7IHHzd4F6 M2\nflczKGh? MnTyTWM2OCB;IECuNFIh|ryP NI\LZoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
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MV4-11 MVfDfZRwfG:6aXPpeJkh[XO|YYm= NH7XS|Q1KGSjeYO= M{GzZ2lEPTBiPTCwMlAzPCEQvF2= NInXemU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
MV4-11 MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXL1W|FzPCCmYYnz M2C5V2lEPTBiPTCwMlAzPCEQvF2= M1nFT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
Rosetta2 DE3 NET0NodHfW6ldHnvckBie3OjeR?= MUezNEBucW6| NXnJeJhSUUN3MDC9JFAvODJ6NzFOwG0> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
NALM16 NGLhPGFEgXSxdH;4bYNqfHliYYPzZZk> MUW1JIRigXN? MXfFR|UxKD1iMD6wN{DPxE1? NH3Bc3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
BL21(DE3)-R3-pRARE2 MVjGeY5kfGmxbjDhd5NigQ>? M3XTXFMxKG2rboO= NIDwbmxKSzVyIE2gNE4xOzNizszN MoPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzOUW3NlMoRjJ6MUm1O|I{RC:jPh?=
BL21(DE3) M12wSmZ2dmO2aX;uJIF{e2G7 Mo\iT4QhRSByLkCzOEDPxE1? M1;hclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
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Rosetta2 DE3 M33vRWZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? M3H4ZVMxKG2rboO= MU\JR|UxKD1iMD6wOFY4KM7:TR?= M3vWd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
MOLM13 Ml\qR5l1d3SxeHnjbZR6KGG|c3H5 Mn7hOEBl[Xm| NYHGNZNYUUN3MDC9JFAvODV4IN88US=> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4M{S4O{c,Ojh2NkO0PFc9N2F-
MOLM13 MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3\vVlQh\GG7cx?= M3uxTmlEPTBiPTCwMlA2PiEQvF2= Mk\tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
HL60 MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MYG3NkBp NWPhZ29LUUN3MDC9JFAvODZizszN M4HEUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUewNFI1Lz5{OUG3NFAzPDxxYU6=
HL60 MnfZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4fNZ|Mh\GG7cx?= MYDHR|UxKD1iMD6wOkDPxE1? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3N{C5PUc,Ojl4NUewPVk9N2F-
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Raji NGLxXYdHfW6ldHnvckBie3OjeR?= NX[4b45VUUN3MDC9JFAvODZizszN M4rOZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
Raji NEnw[5lHfW6ldHnvckBie3OjeR?= NGXtWZE1KGh? NIfEN4lKSzVyIE2gNE4xPjlizszN MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEe1PEc,OjR7MEC3OVg9N2F-
MM1S MnW5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MYW3NkBp NUXLeW46UUN3MDC9JFAvODZ7MTFOwG0> MnHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3MkW0N|UoRjJ7NUK1OFM2RC:jPh?=
BL21 (DE3)-codon plus-RIL MV;GcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 NUXoS5BR[nliZnz1c5Jme2OnbnPlJIFvcXOxdILvdJkh[XO|YYm= M2fpcWlEPTBiPTCwMlA4KM7:TR?= NH3CV4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW4OlcyQCd-Mki1PFY4OTh:L3G+
22Rv1 M3fvRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWHYR5BYQTZiaB?= NX7BRYd6UUN3MDC9JFAvODdzIN88US=> NV;Qdo5tRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
22Rv1 M1O0dWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFWwSIcyOiCq NGflTW9KSzVyIE2gNE4xPzFizszN NHnpSmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
MV4-11 M1zONmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUe5UVg2UUN3MDC9JFAvODd{IN88US=> NYjON2RJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPVU4OjNpPkK4NVk2PzJ|PD;hQi=>
BL21(DE3)-R3-pRARE2 NXm3WYlRTnWwY4Tpc44h[XO|YYm= NEG2N2c{OCCvaX7z MlO5TWM2OCB;IECuNFc4KM7:TR?= NWDpWVl4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
BL21(DE3)-R3-pRARE2 NFXvV|NHfW6ldHnvckBie3OjeR?= MXmzNEBucW6| M1\Fe2lEPTBiPTCwMlA4PyEQvF2= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF7NUeyN{c,OjhzOUW3NlM9N2F-
BL21(DE3)-R3-pRARE2 NHz3ZW9HfW6ldHnvckBie3OjeR?= MYSzNEBucW6| NUfPWZJtUUN3MDC9JFAvODd5IN88US=> M1HTZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{e2PFM1Lz5{OUe3Olg{PDxxYU6=
MV4-11 Mn6zR5l1d3SxeHnjbZR6KGG|c3H5 MV2yOEBp NV\seHZLT0l3MDC9JFAvODhizszN M1OyblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUmxN|Y{Lz5{NkG5NVM3OzxxYU6=
MV411 M4[0bGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWW3NkBp NYHHRodrUUN3MDC9JFAvODhizszN NWPXOVlsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkizNVQ2OTNpPkK4N|E1PTF|PD;hQi=>
TY82 MnXqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnSzO|IhcA>? NUTuVlRIUUN3MDC9JFAvODhyODFOwG0> NIHmbHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWyOVQ{PSd-Mkm1NlU1OzV:L3G+
HL60 NGnwbYtHfW6ldHnvckBie3OjeR?= MVeyOEBp NXHoPHRoUUN3MDC9JFAvODh4IN88US=> M{PjOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
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BL21(DE3) NWP2SJl1TnWwY4Tpc44h[XO|YYm= MkjVT4QhRSByLkC5NkDPxE1? M3j6cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
BL21(DE3)-R3-pRARE2 MmLTSpVv[3Srb36gZZN{[Xl? NXHX[25VU2RiPTCwMlEh|ryP M2[xTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUm1NFA4Lz5{OEW5OVAxPzxxYU6=
HT-29 M3HaN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGLuOlE4OiCq NYjYZoRbUUN3MDC9JFAvOTB2IN88US=> NELzcZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1PVQzQCd-MkW1OVk1Ojh:L3G+
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LNCAP cells NWLV[ZRjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NXftNnpNUUN3MDC9JFAvOTB7NjFOwG0> MnToQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
T cells M2jqW2Z2dmO2aX;uJIF{e2G7 MnL1NlQhcA>? M{DNd2lEPTBiPTCwMlEyKM7:TR?= MnG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh|MUS1NVMoRjJ6M{G0OVE{RC:jPh?=
HL60 NInpS5pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mm\WO|IhcA>? MWfJR|UxKD1iMD6xNUDPxE1? NF:zcVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki2PVE6PCd-Mk[4OlkyQTR:L3G+
BL21(DE3) NUPtfmZzTnWwY4Tpc44h[XO|YYm= M2\KXWlEPTBiPTCwMlEzKM7:TR?= NXnFXlhqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
BL21(DE3) M4\icGZ2dmO2aX;uJIF{e2G7 Mne2Nk42KGh? NWixcJVwUUN3MDC9JFAvOTJizszN NFfpclI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
H1299 NX;JcW5nTnWwY4Tpc44h[XO|YYm= M1TjTVI1KGh? NVH0OXpzTUN3MDC9JFAvOTV|IN88US=> NYDCSXBoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFk2OjFpPkK4PVQ6PTJzPD;hQi=>
HD-MB03 M2TzfGN6fG:2b4jpZ4l1gSCjc4PhfS=> NUHSPVdOPSCmYYnz NXHZeXBRTUN3MDC9JFAvOTZizszN MkTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
LNCAP MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NHW4SWg6PiCq NUnHN4VxUUN3MDC9JFAvOTZizszN NWrBVGNCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
Hs578T NVTqSmVuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NH;nNIQyOiCq M3rxdmlEPTBiPTCwMlE3KM7:TR?= MmCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MV4-11 NWjm[4VXSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MXSxNkBp MljQTWM2OCB;IECuNVYh|ryP MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
LNCAP MorYRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MVWxNkBp NGeyb4pKSzVyIE2gNE4yPiEQvF2= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2MUO3NUc,Ojl3NEGzO|E9N2F-
C4-2B MomxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MYO5OkBp NETnN3FKSzVyIE2gNE4yQSEQvF2= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
C4-2B NF\CVmdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MX6xNkBp M3;Rd2lEPTBiPTCwMlE6KM7:TR?= NXPERY5{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
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MV4-11 M{ftcmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M1vFfWlEPTBiPTCwMlI1KM7:TR?= NGj5bmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G0Nlc2OSd-MkexOFI4PTF:L3G+
MV4-11 MmfUR5l1d3SxeHnjbZR6KGG|c3H5 M3jEPFczKGh? MYrJR|UxKD1iMD6yOFIh|ryP MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVzN{CxNUc,OjN3MUewNVE9N2F-
MX1 NX\3N3VmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MkO1O|IhcA>? M{XhdGVEPTBiPTCwMlI2PCEQvF2= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl2OUWyNUc,Ojh7NEm1NlE9N2F-
HT-29 NWr5RYYySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M2\vVFczKGh? M3;qO2lEPTBiPTCwMlI5KM7:TR?= Mk\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
HFL1 NYnuXJI3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NXzmdYdnOTJiaB?= NG\WdXVKSzVyIE2gNE4zQSEQvF2= NXe3eJpQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MDA-MB-231 NEnpTG5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3fpbFMh\GG7cx?= MoXRS2M2OCB;IECuN{DPxE1? NXrRZm5FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
MV4-11 NVW2NHZxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NHyxbYI1QCCq M3HtR2VEPTBiPTCwMlM{OTF|IN88US=> MmHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OUWwNFcoRjJ6NUm1NFA4RC:jPh?=
K562 M3jaPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWXoU2xHUUN3MDC9JFAvPjRizszN MlfjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNEK3OVEoRjJ5MUSyO|UyRC:jPh?=
HL60 MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M1LSW|Q5KGh? MVXFR|UxKD1iMD63OFE{OSEQvF2= MkDzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OUWwNFcoRjJ6NUm1NFA4RC:jPh?=
NCI-H1975 MnTYRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnrNNVIhcA>? M3\zeWlEPTBiPTCxMlI{KM7:TR?= M2PjUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
SAE Mn[3SpVv[3Srb36gZZN{[Xl? M{WxTFQhcA>? MVPJR|UxKD1iMT6zPEDPxE1? NYC2WZlzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
SAE MWDGeY5kfGmxbjDhd5NigQ>? MmHmOEBp MkXITWM2OCB;IEGuOFkh|ryP NYDS[3lORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
SAE NEXCSXdHfW6ldHnvckBie3OjeR?= NFL2WVk1KGh? MofqTWM2OCB;IEGuOVEh|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ2OUe0NUc,Ojl4NEm3OFE9N2F-
U2OS NH7rWIxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUOxNkBp MYXJR|UxKD1iMT62NkDPxE1? M2jjbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
SAE MXPGeY5kfGmxbjDhd5NigQ>? NWXXXox6PCCq NYfZXGdDUUN3MDC9JFEvPjNizszN M1PuS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkS5O|QyLz5{OU[0PVc1OTxxYU6=
A549 MWXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mn7QNVIhcA>? NUjRSGt6UUN3MDC9JFEvPjdizszN M12zblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
MCF7 MkLRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVmzJIRigXN? NGnydHNISzVyIE2gNU44KM7:TR?= Ml3TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
DU145 MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MojtPVYhcA>? NFXMfXpKSzVyIE2gNk42OiEQvF2= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
DU145 NFToPXJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYryZWhPOTJiaB?= MkXGTWM2OCB;IEKuOVIh|ryP MkTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
T47D NIi2O|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXizJIRigXN? MXrHR|UxKD1iMj64JO69VQ>? NVzjXoFXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
PC3 MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NGi5dXo6PiCq NELyWVVKSzVyIE2gN{4xOSEQvF2= MlnLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
PC3 NUDGc2p6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NHXFXVMyOiCq Ml3JTWM2OCB;IEOuNFEh|ryP Mmf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
HeLa MYPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MYixNkBp MVzJR|UxKD1iMz63OkDPxE1? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
K562 NY\rXpBiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHrifVc{KGSjeYO= MlHGS2M2OCB;IEOuPEDPxE1? M4fuVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
A2780 M4PqUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH7OcZo{KGSjeYO= NGLRSHZISzVyIE2gOEDPxE1? M4TFe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
HL60 NH3QZpVEgXSxdH;4bYNqfHliYYPzZZk> NXuydIlROjRiaB?= MkLCTWM2OCB;IEWuN{DPxE1? M{T0XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Mk[2PVk6Lz5{N{K2Olk6QTxxYU6=
MV4-11 NVnWZmw1S3m2b4TvfIlkcXS7IHHzd4F6 MVuyOEBp NHrsXZdKSzVyIE2gOk41KM7:TR?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJ4Nkm5PUc,Ojd{Nk[5PVk9N2F-
K562 M1:5TmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWS3NkBp M4\6T2lEPTBiPTC5MlEzKM7:TR?= NEC4U2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
OVCAR5 M3PoZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M132WVMh\GG7cx?= M3PDfmdEPTBiPTCxNkDPxE1? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
In vivo (+)-JQ1 (50 mg/kg) inhibits tumors growth in mice with NMC 797 xenografts. (+)-JQ1 (50 mg/kg) results in effacement of NUT nuclear speckles in mice with NMC 797 xenografts, consistent with competitive binding to nuclear chromatin. (+)-JQ1 (50 mg/kg) induces strong (grade 31) keratin expression in NMC 797 xenografts. (+)-JQ1 (50 mg/kg) promotes differentiation, tumor regression and prolonged survival in mice models of NMC xenografts. [1] (+)-JQ1 (50 mg/kg) results in a significant prolongation in overall survival of SCID-beige mice orthotopically xenografted after intravenous injection with MM.1S-luc+ cells compared to vehicle-treated animals. [2] (+)-JQ1 (50 mg/kg i.p.) leads to a highly significant increase in survival of mice bearing Raji xenografts. [3]

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: MC 11060 cells
  • Concentrations: ~500 nM
  • Incubation Time: 48 hours
  • Method:

    Cells are seeded into white, 384-well microtiter plates at 500 cells per well in a total volume of 50 μL media. The 797, TT and TE10 cells are grown in DMEM containing 1% penicillin/streptomycin and 10% FBS. The Per403 cells are grown in DMEM containing 1 % penicillin/streptomycin and 20% FBS. Patient-derived NMC 11060 cells are grown in RPMI with 10% FBS and 1% penicillin/streptomycin. (+)-JQ1 is delivered to microtiter assay plates by robotic pin transfer. Following a 48 hours incubation at 37℃, cells are lysed and wells are assessed for total ATP content using a commercial proliferation assay. Replicate measurements are analyzed with respect to dose and estimates of IC50 are calculated by logistic regression (GraphPad Prism).


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: Mice bearing NMC 797 xenografts
  • Dosages: 50 mg/kg
  • Administration: intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 456.99
Formula

C23H25ClN4O2S

CAS No. 1268524-70-4
Storage powder
in solvent
Synonyms N/A
Smiles CC1=C(SC2=C1C(=NC(C3=NN=C(N32)C)CC(=O)OC(C)(C)C)C4=CC=C(C=C4)Cl)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    How can I reconstitute the compound for in vivo injection?

  • Answer:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID