(+)-JQ1

For research use only.

Catalog No.S7110

295 publications

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.

Selleck's (+)-JQ1 has been cited by 295 publications

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Biological Activity

Description (+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.
Features (+)-JQ1 is more effective than (-)-JQ1.
Targets
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
In vitro

(+)-JQ1 enantiomer binds directly into the Kac binding site of BET bromodomains. (+)-JQ1 (500 nM) binds BRD4 competitively with chromatin resulting in differentiation and growth arrest of NMC cells. (+)-JQ1 (500 nM) attenuates rapid proliferation of NMC 797 and Per403 cell lines as demonstrated by reduced Ki67 staining. (+)-JQ1 (500 nM) potently decreases expression of both BRD4 target genes in NMC 797 cells. (+)-JQ1 inhibits cellular viability with IC50 of 4 nM in NMC 11060 cells. [1] (+)-JQ1 results in robust inhibition of MYC expression in MM cell lines. (+)-JQ1 inhibits proliferating of KMS-34 and LR5 with IC50 of 68 nM and 98 nM, respectively. (+)-JQ1 (500 nM)-treated MM.1S cells results in a pronounced decrease in the proportion of cells in S-phase, with a concomitant increase in cells arrested in G0/G1. (+)-JQ1 (500 nM) results in pronounced cellular senescence by beta-galactosidase staining. (+)-JQ1 (800 nM) exposure leads to a significant reduction in cell viability among the majority of CD138+ patient-derived MM samples tested. [2] (+)-JQ1 inhibits growth of LP-1 cells with GI50 of 98 nM. (+)-JQ1 (625 nM) results in an increase in the percentage of LP-1 cells in G0/G1. (+)-JQ1 (500 nM) suppresses the expression of MYC, BRD4 and CDK9 in LP-1 cells. [3] (+)-JQ1 (1 μM) activates HIV transcription in latently infected Jurkat T cells. (+)-JQ1 (50 μM) stimulates predominantly Tat-dependent HIV transcription in both Jurkat and HeLa cells. (+)-JQ1 (5 μM) induces Brd4 dissociation enables Tat to recruit SEC to HIV promoter and induce Pol II CTD phosphorylation and viral transcription in J-Lat A2 cells. JQ1 enables Tat to increase CDK9 T-loop phosphorylation and partially dissociates P-TEFb from 7SK snRNP in Jurkat T cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MXHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NVPCT5VPOjVyL{WwNE8yODByIH7N M{j1NlI1NzR6L{eyJIg> M3P2d2ROW09? NX\YdXBqcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgbY4h[m:2aDDkc5NmNSCjbnSgeIlu\S1iZHXw[Y5l\W62IH3hco5meg>? MlTEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEe4PFEoRjJ4N{C3PFgyRC:jPh?=
BCPAP NVT2VWZ5S2WubDDWbYFjcWyrdImgRZN{[Xl? MVqyOVAwPTByL{GwNFAhdk1? MoWwNlQwPDhxN{KgbC=> NVn1bHRwTE2VTx?= MX3pcohq[mm2czDj[YxtKH[rYXLpcIl1gSCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy NETv[lM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewO|g5OSd-Mk[3NFc5QDF:L3G+
K1  MV;D[YxtKEO7Y3zlJGF{e2G7 M{DXW|I2OC93MECvNVAxOCCwTR?= NVG0VZdNPzJiaB?= NVjMTnA6TE2VTx?= MlnSZZJz\XO2czDj[YxtKGO7Y3zlJIF1KEdyL1exJJBp[XOn NUXzTZZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFc5QDFpPkK2O|A4QDhzPD;hQi=>
BCPAP M4fsO2NmdGxiQ4njcIUhSXO|YYm= NIrxW2QzPTBxNUCwM|ExODBibl2= NH3HVpU4OiCq NXznbWRvTE2VTx?= M123SYFzemW|dIOgZ4VtdCCleXPs[UBifCCJMD;HNUBxcGG|ZR?= MnfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEe4PFEoRjJ4N{C3PFgyRC:jPh?=
Hep3B NUfJWXJST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYP2bGdLOC1zMDFOwG0> MlXhOUBl MYTEUXNQ MUnJR|UxRTBwMEig{txO MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HCCLM3 M335c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV2wMVExKM7:TR?= MkOxOUBl NEjKOm1FVVOR NETDUYVKSzVyPUCuNVQh|ryP NYLEcG5yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HuH7 Mnj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;RUXlqOC1zMDFOwG0> Mlq5OUBl MnHMSG1UVw>? NXLjd2VCUUN3ME2wMlIyKM7:TR?= NV3GUJRPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HepG2 M3izOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHnuW|cxNTFyIN88US=> Mm\ZOUBl NYno[GJwTE2VTx?= NX3tRm5{UUN3ME2wMlM1KM7:TR?= M3:4VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
SMMC7721 M1ThTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLYNE0yOCEQvF2= MV:1JIQ> NVzK[2hWTE2VTx?= MUTJR|UxRTBwNEGg{txO NY\1doI1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
BEL7402 NInLblZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXywMVExKM7:TR?= MnrZOUBl MlnWSG1UVw>? NX3QfnFIUUN3ME2wMlQ4KM7:TR?= MkTkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
MHCC97H NIHKO3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfTflI1OC1zMDFOwG0> M{nVXFUh\A>? NGHtS|ZFVVOR M2ntUmlEPTB;MD60NUDPxE1? NHrueoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
Hep3B NXvHbGxQS2WubDDDfYNt\SCDc4PhfS=> NES0dY8xNjFxMD61M|IvPSEQvF2= M2HIZlQ5KGh? MYDEUXNQ NWqzVmFYdGWjZIOgeI8h[SC|dXLzeIFvfGmjbDDhZ4N2dXWuYYTpc44hd2ZiSFPDJINmdGy|IHnuJJN2[i2JMTDwbIF{\cLi MojoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HCCLM3 Mm[xR4VtdCCFeXPs[UBCe3OjeR?= NFHWOpQxNjFxMD61M|IvPSEQvF2= NULSWGE5PDhiaB?= NG\4V2dFVVOR M4Tvd4xm[WS|IITvJIEhe3Wkc4ThcpRq[WxiYXPjeY12dGG2aX;uJI9nKEiFQzDj[YxteyCrbjDzeYIuTzFicHjhd4XDqA>? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
Hep3B NXL6Z41xSXCxcITvd4l{KEG|c3H5 MnO0NE4yNzBwNT:yMlUh|ryP M1nP[lQ5KGh? MY\EUXNQ M37JcYFkfGm4YYTld{Bk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOTDlfJBz\XO|aX;uJIFv\CCrbnT1Z4VlKFCDUmCgZ4xm[X[jZ3WgZZMhf2WubDDhd{BkgXSxY3jyc41mKGNicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21iZoLvcUBucXSxY3jvcoRzcWF? MkLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HCCLM3 MmPWRZBweHSxc3nzJGF{e2G7 NVXlboR6OC5zL{CuOU8zNjVizszN MWi0PEBp M4nDdmROW09? NUXzeo1m[WO2aY\heIV{KGOjc4Dhd4UuOyCjbnSgZ4F{eGG|ZT25JIV5eHKnc4Ppc44h[W6mIHnu[JVk\WRiUFHSVEBkdGWjdnHn[UBieyC5ZXzsJIF{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnueI8hfGinIHP5eI9xdGG|bTDmdo9uKG2rdH;jbI9v\HKrYR?= NULPem94RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
A549 NVLKNYhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVewMlEuOTBizszN NFW0TpM4OiCq MXTpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MmPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H157 NX7ufItOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfZcXQxNjFvMUCg{txO NUHK[5VmPzJiaB?= MojHbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NVLkcG1wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 NYfN[5Y4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmflNE4yNTFyIN88US=> MYe3NkBp MlXqbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M{KzNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
A549 NXLSPJE3TnWwY4Tpc44hSXO|YYm= NXq4WVhqOS9{LkWvOUDPxE1? NY\CNHB5OTJiaB?= NXvMS5RkyqC5ZXHrcJkh\GWlcnXhd4VlKEKlbD2yJIxmfmWucx?= NXjNPJNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 NWW4eGY3TnWwY4Tpc44hSXO|YYm= NGDDcJEyNzJwNT:1JO69VQ>? MYSxNkBp NVqyeohYyqC5ZXHrcJkh\GWlcnXhd4VlKEKlbD2yJIxmfmWucx?= NXHuW3JFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H157 M3zFVWZ2dmO2aX;uJGF{e2G7 MVWxM|IvPS93IN88US=> NXjkdJR{OTJiaB?= NX:xW3Bo\GWlcnXhd4VlKESUNDDlfJBz\XO|aX;u MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
H1299 NECyWIJHfW6ldHnvckBCe3OjeR?= NUjyTWV2OS9{LkWvOUDPxE1? M{XQcVEzKGh? NIC4OFRl\WO{ZXHz[YQhTFJ2IHX4dJJme3Orb36= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
C8161 MXXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2DH[VAuOiEQvF2= NUi4b|VXPCCm MVrEUXNQ NFqxV5Vl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M3G2RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel285 MoP1R4VtdCCYaXHibYxqfHliQYPzZZk> NITlTGIxNTJizszN NFHsfpY1KGR? MXPEUXNQ M3LwWoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MlvPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel290 NFLBO2JE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MX2wMVIh|ryP NVW1XGZNPCCm MknGSG1UVw>? NVPzdY1F\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MnnaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
92.1 NH\DNIZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MYiwMVIh|ryP MXS0JIQ> NX;sfHlqTE2VTx?= M3vqNIRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= Mke4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Omm1.3 NE\OVplE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MoDMNE0zKM7:TR?= M13mTlQh\A>? NEfSNWtFVVOR M1X3N4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MoX3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel202 M4HSSGNmdGxiVnnhZoltcXS7IFHzd4F6 MVSwMVIh|ryP MkT0OEBl NHToT5pFVVOR M121b4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NUfsZXh7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Mel270 NGHl[HlE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M3fEN|AuOiEQvF2= MWS0JIQ> MlPFSG1UVw>? MYjk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NG\BUpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Omm1 Mn\GR4VtdCCYaXHibYxqfHliQYPzZZk> NUezTmxNOC1{IN88US=> NV\UeHZEPCCm MnfRSG1UVw>? NXTpWWxr\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NFL1e4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
92.1 MXfBdI9xfG:|aYOgRZN{[Xl? NXz2eHAzPTByIH7N MmLLOFghcA>? M1v2emROW09? NWfLPY1KcW6mdXPld{BieG:ydH;zbZM> M1SzN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Omm1.3 NVzWbZJESXCxcITvd4l{KEG|c3H5 Mnr5OVAxKG6P M{jmOFQ5KGh? MVnEUXNQ MlLubY5lfWOnczDhdI9xfG:|aYO= MmXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
92.1 M4q2UGNmdGxiQ4njcIUhSXO|YYm= M1vuSlUxOCCwTR?= Ml7BNlQwPDhxN{KgbC=> M{jKPWROW09? NIXjNIJqdmS3Y3XzJJRp\SClZXzsJIFk[3WvdXzheIlwdiCjdDDzeYIuTzIEoB?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Omm1.3 MkLQR4VtdCCFeXPs[UBCe3OjeR?= MlS0OVAxKG6P MoXBNlQwPDhxN{KgbC=> MmT2SG1UVw>? M3nVT4lv\HWlZYOgeIhmKGOnbHygZYNkfW23bHH0bY9vKGG2IIP1Zk1IOcLi NGPMWpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
A549 NUnWfIVOTnWwY4Tpc44hSXO|YYm= MlzsNVAxNzRyMD:xNFAxKG6P M3TVdlI1KGh? MmLDeZBz\We3bHH0[ZMh[W6mIHHjeIl3[XSnczDTTXJVOQ>? M1\WUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkGyNVk6Lz5{NkKxNlE6QTxxYU6=
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SU-DHL-10 MkHDRZBweHSxc3nzJGF{e2G7 Mkf3NVczNzJ3MDDuUS=> M1jvTVdl MVXEUXNQ NWrIToF1cW6lcnXhd4V{KGOjc4Dhd4UuOy95IHHjeIl3cXS7wrDzbYdvcW[rY3HueIx6 NWDmb41kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNFkzQTVpPkK1NFA6Ojl3PD;hQi=>
Rosetta2 DE3 NFf2emRHfW6ldHnvckBie3OjeR?= M2e1UGtlKD1iMD6wNFYzKM7:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 M{S2bGZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? NVvNfIRxOzBibXnudy=> NGTU[mdMcSB;IECuNFA3PiEQvF2= NUjvXJFKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 MknuSpVv[3Srb36gZZN{[Xl? MVLL[EA:KDBwMEC2O{DPxE1? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 NWD4UGRXTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= NInxSnM{OCCvaX7z NWf5OopVU2liPTCwMlAxPzZizszN NUfYeYQ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
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Rosetta2 DE3 MlSzSpVv[3Srb36gZZN{[Xl? MoPrT4QhRSByLkCxNVch|ryP MlrhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
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Rosetta2 DE3 M1jHemZ2dmO2aX;uJIF{e2G7 NGT0XI5M\CB;IECuNFEzQCEQvF2= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 NWjZWFdtTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= NXHZfZp[OzBibXnudy=> MXHLbUA:KDBwMEGzNkDPxE1? MkfkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
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MV4-11 NUXiOJpJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mmf6O|IhcA>? NXq5UI9[UUN3MDC9JFAvODJ|IN88US=> MknOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NUm0NlgoRjJ3NUW5OFI5RC:jPh?=
MV4-11 Mm\WR5l1d3SxeHnjbZR6KGG|c3H5 NWDQOJlEPCCmYYnz NXKyNWNtUUN3MDC9JFAvODJ2IN88US=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4M{S4O{c,Ojh2NkO0PFc9N2F-
MV4-11 MnHDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX3NSGhMPCCmYYnz MXLJR|UxKD1iMD6wNlQh|ryP NXTH[YhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NH;Mc25HfW6ldHnvckBie3OjeR?= M3;6eVMxKG2rboO= NF\lNGlKSzVyIE2gNE4xOjh5IN88US=> NHPrdFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
NALM16 NH7l[oNEgXSxdH;4bYNqfHliYYPzZZk> MoXROUBl[Xm| NWjWR492TUN3MDC9JFAvODNizszN MkXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
BL21(DE3)-R3-pRARE2 MnXISpVv[3Srb36gZZN{[Xl? MVSzNEBucW6| M3K2W2lEPTBiPTCwMlA{OyEQvF2= NYjpdVdZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPVU4OjNpPkK4NVk2PzJ|PD;hQi=>
BL21(DE3) NGnEPGpHfW6ldHnvckBie3OjeR?= NWLWUnhoU2RiPTCwMlA{PCEQvF2= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
Rosetta2 DE3 NH34VHpHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 NVi3Xo1{OzBibXnudy=> MXHJR|UxKD1iMD6wN|U4KM7:TR?= NVTFe2JlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NXPXNWV7Tmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= MnzzN|AhdWmwcx?= NXHjVHl5UUN3MDC9JFAvODR{MjFOwG0> Mnf2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
Rosetta2 DE3 MXjGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 MWmzNEBucW6| MWDJR|UxKD1iMD6wOFY4KM7:TR?= NID2VZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
MOLM13 Mmm3R5l1d3SxeHnjbZR6KGG|c3H5 NFK5XIw1KGSjeYO= NFzObIVKSzVyIE2gNE4xPTZizszN Ml:3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
MOLM13 NXfuZlNzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1PDXFQh\GG7cx?= NUezWGQ3UUN3MDC9JFAvODV4IN88US=> NYfjVoZkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
HL60 NXjlVll1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M1TvW|czKGh? MnTITWM2OCB;IECuNFYh|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
HL60 NXrXfGpOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXzOdHdtOyCmYYnz MnnvS2M2OCB;IECuNFYh|ryP MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3N{C5PUc,Ojl4NUewPVk9N2F-
NALM6 NX\LSYxyS3m2b4TvfIlkcXS7IHHzd4F6 MW[1JIRigXN? MlTGSWM2OCB;IECuNFYh|ryP Mn3QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
Raji MX;GeY5kfGmxbjDhd5NigQ>? Ml73TWM2OCB;IECuNFYh|ryP MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
Raji NYG4XlZtTnWwY4Tpc44h[XO|YYm= NUfNTXVKPCCq NIjDVHNKSzVyIE2gNE4xPjlizszN NYnIO413RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4PThpPkK0PVAxPzV6PD;hQi=>
MM1S MorlRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnvGO|IhcA>? M2XVWGlEPTBiPTCwMlA3QTFizszN MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV{NUSzOUc,Ojl3MkW0N|U9N2F-
BL21 (DE3)-codon plus-RIL NFPVSmdHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 NYDsfJk1[nliZnz1c5Jme2OnbnPlJIFvcXOxdILvdJkh[XO|YYm= NWriUnpsUUN3MDC9JFAvODdizszN MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV6NkexPEc,Ojh3OE[3NVg9N2F-
22Rv1 NXLV[|NkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYnhc2h3QTZiaB?= M{OxfWlEPTBiPTCwMlA4OSEQvF2= NVy0Zm04RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
22Rv1 MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M1zKZlEzKGh? NWW2bFFkUUN3MDC9JFAvODdzIN88US=> Ml\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
MV4-11 M1O4OGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M1zUb2lEPTBiPTCwMlA4OiEQvF2= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF7NUeyN{c,OjhzOUW3NlM9N2F-
BL21(DE3)-R3-pRARE2 MoXiSpVv[3Srb36gZZN{[Xl? NHS3Rpc{OCCvaX7z MnXwTWM2OCB;IECuNFc4KM7:TR?= NXP5[nFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
BL21(DE3)-R3-pRARE2 MX7GeY5kfGmxbjDhd5NigQ>? MXmzNEBucW6| NXTGVXJ1UUN3MDC9JFAvODd5IN88US=> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF7NUeyN{c,OjhzOUW3NlM9N2F-
BL21(DE3)-R3-pRARE2 NVPndpg2TnWwY4Tpc44h[XO|YYm= M4rXRVMxKG2rboO= NITIWXJKSzVyIE2gNE4xPzdizszN NWXmWJZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3O|Y5OzRpPkK5O|c3QDN2PD;hQi=>
MV4-11 MXzDfZRwfG:6aXPpeJkh[XO|YYm= M3HCcFI1KGh? MkjsS2k2OCB;IECuNFgh|ryP MnHEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzOUGzOlMoRjJ4MUmxN|Y{RC:jPh?=
MV411 MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M4m0VlczKGh? NWHX[VlHUUN3MDC9JFAvODhizszN NHjNeHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
TY82 NG\x[HRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mm\tO|IhcA>? NXPNWYI6UUN3MDC9JFAvODhyODFOwG0> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV{NUSzOUc,Ojl3MkW0N|U9N2F-
HL60 NIDLS5hHfW6ldHnvckBie3OjeR?= MVmyOEBp NGG3SndKSzVyIE2gNE4xQDZizszN NFnMc2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+
697 MXzDfZRwfG:6aXPpeJkh[XO|YYm= NUDVTGxUPSCmYYnz MlnvSWM2OCB;IECuNFkh|ryP MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
Loucy MoG3R5l1d3SxeHnjbZR6KGG|c3H5 MYC1JIRigXN? Mn\zSWM2OCB;IECuNFkh|ryP MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
BL21(DE3) MnTxSpVv[3Srb36gZZN{[Xl? MX\L[EA:KDBwMEmyJO69VQ>? M1i2bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
BL21(DE3)-R3-pRARE2 MnvnSpVv[3Srb36gZZN{[Xl? MmjOT4QhRSByLkGg{txO M1q1eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUm1NFA4Lz5{OEW5OVAxPzxxYU6=
HT-29 NWX3XIxkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFLFOnI4OiCq Mk\zTWM2OCB;IECuNVA1KM7:TR?= NVX1T|BPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
MM1S M3PHc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4HOOlczKGh? M1PiRWlEPTBiPTCwMlExQSEQvF2= MkfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NUm0NlgoRjJ3NUW5OFI5RC:jPh?=
LNCAP cells NG\QbWlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MVTJR|UxKD1iMD6xNFk3KM7:TR?= NYOyR3hxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
T cells MWTGeY5kfGmxbjDhd5NigQ>? MmW2NlQhcA>? NIfpdmlKSzVyIE2gNE4yOSEQvF2= M{Xyd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6M{G0OVE{Lz5{OEOxOFUyOzxxYU6=
HL60 NXPkOm5ESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEP0U|E4OiCq NVrSfm1{UUN3MDC9JFAvOTFizszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh4OUG5OEc,OjZ6NkmxPVQ9N2F-
BL21(DE3) M4nZ[WZ2dmO2aX;uJIF{e2G7 NGTIWJBKSzVyIE2gNE4yOiEQvF2= MnPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
BL21(DE3) NWr6UXJiTnWwY4Tpc44h[XO|YYm= NIizXZUzNjViaB?= MVHJR|UxKD1iMD6xNkDPxE1? NF:zWYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
H1299 NH;GXYhHfW6ldHnvckBie3OjeR?= NELOS5UzPCCq MmPSSWM2OCB;IECuNVU{KM7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl2OUWyNUc,Ojh7NEm1NlE9N2F-
HD-MB03 MV;DfZRwfG:6aXPpeJkh[XO|YYm= NFXsRWM2KGSjeYO= NX74fXRsTUN3MDC9JFAvOTZizszN NUXmZ4pxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
LNCAP M4fwS2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVm5OkBp NYrLUm1SUUN3MDC9JFAvOTZizszN Mne3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
Hs578T M3jCbGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NILDcW8yOiCq NYDu[IZ7UUN3MDC9JFAvOTZizszN M4q0eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
MV4-11 NXXab5NVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NI\Ne4QyOiCq NUT1XXUzUUN3MDC9JFAvOTZizszN NXvPPHc{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxO|AxOjRpPkK5NVcxODJ2PD;hQi=>
LNCAP NYny[WVVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mk\GNVIhcA>? NFi1WmhKSzVyIE2gNE4yPiEQvF2= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2MUO3NUc,Ojl3NEGzO|E9N2F-
C4-2B M13RVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVq5OkBp Mm\HTWM2OCB;IECuNVkh|ryP M3LKRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
C4-2B M1roemFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWCzfoF5OTJiaB?= MkXuTWM2OCB;IECuNVkh|ryP MmHkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
Vero E6 MVTBcpRqfmm{YXygZYN1cX[rdImgZZN{[Xl? MXm0PEBp MmLGTWM2OCB;IECuNVkzPzVizszN MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OjN3M{i1PUc,OzJ|NUO4OVk9N2F-
MCF7 NWjXWmQ1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M3nj[|EzKGh? NXGyT5FkUUN3MDC9JFAvOiEQvF2= NI\RR|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
MV4-11 MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXXtUGlqUUN3MDC9JFAvOjRizszN NGHRT5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G0Nlc2OSd-MkexOFI4PTF:L3G+
MV4-11 NEjvPYlEgXSxdH;4bYNqfHliYYPzZZk> MV63NkBp Mmq0TWM2OCB;IECuNlQzKM7:TR?= M4rXS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUG3NFEyLz5{M{WxO|AyOTxxYU6=
MX1 M1TpeWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NHHidVI4OiCq M{n6cmVEPTBiPTCwMlI2PCEQvF2= M4rXOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUS5OVIyLz5{OEm0PVUzOTxxYU6=
HT-29 NFn5b4NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NXPm[2s3PzJiaB?= NVzifmc4UUN3MDC9JFAvOjhizszN NXS1cmF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
HFL1 MmnjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NXzqcYpQOTJiaB?= NXTuWnVDUUN3MDC9JFAvOjlizszN MnXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MDA-MB-231 NWPmPXFNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlzRN{Bl[Xm| M4HpVWdEPTBiPTCwMlMh|ryP NX7XZ4x1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
MV4-11 NULZOFhpSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEHSR4U1QCCq NXXE[YptTUN3MDC9JFAvOzNzMUOg{txO NXvIeY4{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1PVUxODdpPkK4OVk2ODB5PD;hQi=>
K562 MkfmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MYTJR|UxKD1iMD62OEDPxE1? M4XEbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUSyO|UyLz5{N{G0Nlc2OTxxYU6=
HL60 NFizdZBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MknkOFghcA>? NEHRWXBGSzVyIE2gNE44PDF|MTFOwG0> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV7NUCwO{c,Ojh3OUWwNFc9N2F-
NCI-H1975 NHv5WoJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NH;aeGUyOiCq NXr2[YR3UUN3MDC9JFEvOjNizszN MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
SAE MUXGeY5kfGmxbjDhd5NigQ>? NILaR|c1KGh? M1nGc2lEPTBiPTCxMlM5KM7:TR?= NYf6PVNHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
SAE M4mwOmZ2dmO2aX;uJIF{e2G7 M1j3WVQhcA>? NYO5SphyUUN3MDC9JFEvPDlizszN MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ2OUe0NUc,Ojl4NEm3OFE9N2F-
SAE MkHkSpVv[3Srb36gZZN{[Xl? MoPGOEBp M1rKXmlEPTBiPTCxMlUyKM7:TR?= NF;VVpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0PVc1OSd-Mkm2OFk4PDF:L3G+
U2OS NXPVOG5JSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MkD4NVIhcA>? NXXKZYxxUUN3MDC9JFEvPjJizszN M4XjblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
SAE MlzWSpVv[3Srb36gZZN{[Xl? M{TZSVQhcA>? M2e0OGlEPTBiPTCxMlY{KM7:TR?= NYnPWFlXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
A549 NUfrNoVRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NIT4flMyOiCq MW\JR|UxKD1iMT62O{DPxE1? NYXNV292RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MCF7 NIr0NnpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUL3[FI{OyCmYYnz NEjWfJRISzVyIE2gNU44KM7:TR?= NVrJZpFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
DU145 MU\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NF\ISIE6PiCq MYDJR|UxKD1iMj61NkDPxE1? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
DU145 M2XDPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MX[xNkBp NEnaUIhKSzVyIE2gNk42OiEQvF2= NUHMPYhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
T47D NEXWTZZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXftZnVsOyCmYYnz NHW2PWlISzVyIE2gNk45KM7:TR?= Ml[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
PC3 NEDjZpRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MkiyPVYhcA>? M1jtWmlEPTBiPTCzMlAyKM7:TR?= MnXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
PC3 NGfuOm5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3zRelEzKGh? M4W0eWlEPTBiPTCzMlAyKM7:TR?= Ml32QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
HeLa NG\QXodCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUixNkBp MVTJR|UxKD1iMz63OkDPxE1? MoX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
K562 NF;QVGhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnzoN{Bl[Xm| NGrVVW1ISzVyIE2gN{45KM7:TR?= NG\vSFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+
A2780 MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUXhV|JLOyCmYYnz MWLHR|UxKD1iNDFOwG0> NEK0Spk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+
HL60 MWDDfZRwfG:6aXPpeJkh[XO|YYm= M4nK[lI1KGh? Ml3ITWM2OCB;IEWuN{DPxE1? NEjjcFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{K2Olk6QSd-MkeyOlY6QTl:L3G+
MV4-11 MmL0R5l1d3SxeHnjbZR6KGG|c3H5 MnTlNlQhcA>? NYnMN4RiUUN3MDC9JFYvPCEQvF2= NUHO[|VzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkeyOlY6QTlpPkK3NlY3QTl7PD;hQi=>
K562 M3TjfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWS3NkBp M3\tcmlEPTBiPTC5MlEzKM7:TR?= MlzoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh|MUS1NVMoRjJ6M{G0OVE{RC:jPh?=
OVCAR5 M17rNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYXGSHdwOyCmYYnz NFjZNotISzVyIE2gNVIh|ryP NHX1fIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
In vivo (+)-JQ1 (50 mg/kg) inhibits tumors growth in mice with NMC 797 xenografts. (+)-JQ1 (50 mg/kg) results in effacement of NUT nuclear speckles in mice with NMC 797 xenografts, consistent with competitive binding to nuclear chromatin. (+)-JQ1 (50 mg/kg) induces strong (grade 31) keratin expression in NMC 797 xenografts. (+)-JQ1 (50 mg/kg) promotes differentiation, tumor regression and prolonged survival in mice models of NMC xenografts. [1] (+)-JQ1 (50 mg/kg) results in a significant prolongation in overall survival of SCID-beige mice orthotopically xenografted after intravenous injection with MM.1S-luc+ cells compared to vehicle-treated animals. [2] (+)-JQ1 (50 mg/kg i.p.) leads to a highly significant increase in survival of mice bearing Raji xenografts. [3]

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: MC 11060 cells
  • Concentrations: ~500 nM
  • Incubation Time: 48 hours
  • Method:

    Cells are seeded into white, 384-well microtiter plates at 500 cells per well in a total volume of 50 μL media. The 797, TT and TE10 cells are grown in DMEM containing 1% penicillin/streptomycin and 10% FBS. The Per403 cells are grown in DMEM containing 1 % penicillin/streptomycin and 20% FBS. Patient-derived NMC 11060 cells are grown in RPMI with 10% FBS and 1% penicillin/streptomycin. (+)-JQ1 is delivered to microtiter assay plates by robotic pin transfer. Following a 48 hours incubation at 37℃, cells are lysed and wells are assessed for total ATP content using a commercial proliferation assay. Replicate measurements are analyzed with respect to dose and estimates of IC50 are calculated by logistic regression (GraphPad Prism).


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: Mice bearing NMC 797 xenografts
  • Dosages: 50 mg/kg
  • Administration: intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 456.99
Formula

C23H25ClN4O2S

CAS No. 1268524-70-4
Storage powder
in solvent
Synonyms N/A
Smiles CC1=C(SC2=C1C(=NC(C3=NN=C(N32)C)CC(=O)OC(C)(C)C)C4=CC=C(C=C4)Cl)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    How can I reconstitute the compound for in vivo injection?

  • Answer:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID