Home Epigenetics Target Protein Ligand chemical - Epigenetic Reader Domain inhibitor - Autophagy activator - NSD3 inhibitor (+)-JQ1

(+)-JQ1

Catalog No.S7110

For research use only.

(+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

Selleck's (+)-JQ1 has been cited by 317 publications

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Biological Activity

Description (+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family. (+)-JQ1 suppresses cell proliferation via inducing autophagy. (+)-JQ1 inhibits the expression of Nuclear receptor binding SET domain protein 3 (NSD3) target genes.
Features (+)-JQ1 is more effective than (-)-JQ1.
Targets
BRD4 (2) [1]
(Cell-free assay)		 BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
In vitro

(+)-JQ1 enantiomer binds directly into the Kac binding site of BET bromodomains. (+)-JQ1 (500 nM) binds BRD4 competitively with chromatin resulting in differentiation and growth arrest of NMC cells. (+)-JQ1 (500 nM) attenuates rapid proliferation of NMC 797 and Per403 cell lines as demonstrated by reduced Ki67 staining. (+)-JQ1 (500 nM) potently decreases expression of both BRD4 target genes in NMC 797 cells. (+)-JQ1 inhibits cellular viability with IC50 of 4 nM in NMC 11060 cells. [1] (+)-JQ1 results in robust inhibition of MYC expression in MM cell lines. (+)-JQ1 inhibits proliferating of KMS-34 and LR5 with IC50 of 68 nM and 98 nM, respectively. (+)-JQ1 (500 nM)-treated MM.1S cells results in a pronounced decrease in the proportion of cells in S-phase, with a concomitant increase in cells arrested in G0/G1. (+)-JQ1 (500 nM) results in pronounced cellular senescence by beta-galactosidase staining. (+)-JQ1 (800 nM) exposure leads to a significant reduction in cell viability among the majority of CD138+ patient-derived MM samples tested. [2] (+)-JQ1 inhibits growth of LP-1 cells with GI50 of 98 nM. (+)-JQ1 (625 nM) results in an increase in the percentage of LP-1 cells in G0/G1. (+)-JQ1 (500 nM) suppresses the expression of MYC, BRD4 and CDK9 in LP-1 cells. [3] (+)-JQ1 (1 μM) activates HIV transcription in latently infected Jurkat T cells. (+)-JQ1 (50 μM) stimulates predominantly Tat-dependent HIV transcription in both Jurkat and HeLa cells. (+)-JQ1 (5 μM) induces Brd4 dissociation enables Tat to recruit SEC to HIV promoter and induce Pol II CTD phosphorylation and viral transcription in J-Lat A2 cells. JQ1 enables Tat to increase CDK9 T-loop phosphorylation and partially dissociates P-TEFb from 7SK snRNP in Jurkat T cells. [4]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MlrpR4VtdCCYaXHibYxqfHliQYPzZZk> MViyOVAwPTByL{GwNFAhdk1? MnLRNlQwPDhxN{KgbC=> MXjEUXNQ NHPTWFFqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIHTvd4UuKGGwZDD0bY1mNSCmZYDlcoRmdnRibXHucoVz MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
BCPAP NXLPR4xbS2WubDDWbYFjcWyrdImgRZN{[Xl? NYXYV2VjOjVyL{WwNE8yODByIH7N NF3nV3YzPC92OD:3NkBp NGfSUG5FVVOR NILPfpBqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIHTvd4UuKGGwZDD0bY1mNSCmZYDlcoRmdnRibXHucoVz MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
K1  NXzuO483S2WubDDDfYNt\SCDc4PhfS=> NF:1[|IzPTBxNUCwM|ExODBibl2= NF\wSZc4OiCq M{Dzd2ROW09? NGfLUGxienKnc4TzJINmdGxiY4njcIUh[XRiR{CvS|EheGijc3W= M3nwUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP NX3pdlVvS2WubDDDfYNt\SCDc4PhfS=> NH3vcHIzPTBxNUCwM|ExODBibl2= Mlr1O|IhcA>? MVXEUXNQ M{HxdoFzemW|dIOgZ4VtdCCleXPs[UBifCCJMD;HNUBxcGG|ZR?= NVLITXh3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFc5QDFpPkK2O|A4QDhzPD;hQi=>
Hep3B NXTkfGxHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3ez[VAuOTBizszN Ml7BOUBl M{TYcGROW09? NF\4VoVKSzVyPUCuNFgh|ryP NUHueGFTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HCCLM3 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUGzZplwOC1zMDFOwG0> MoSzOUBl MlnqSG1UVw>? M37GZmlEPTB;MD6xOEDPxE1? NHrEb|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HuH7 NXfwZ5JvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYOwMVExKM7:TR?= NF:y[Gs2KGR? MoP0SG1UVw>? M3vBXGlEPTB;MD6yNUDPxE1? M4XkclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HepG2 NGjoO3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{C2[lAuOTBizszN M1fRT|Uh\A>? MnXNSG1UVw>? MWPJR|UxRTBwM{Sg{txO M1ryeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
SMMC7721 NFjYPYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nBNlAuOTBizszN MojyOUBl NHXWVIhFVVOR NX;TflEyUUN3ME2wMlQyKM7:TR?= NHrCVVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
BEL7402 M2\XW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLzT|AxNTFyIN88US=> MWW1JIQ> NY\R[m17TE2VTx?= M4eyT2lEPTB;MD60O{DPxE1? NXPUfY9xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
MHCC97H M3\Hdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWCwMVExKM7:TR?= NUf6OmF3PSCm M4\vXWROW09? M3LhfGlEPTB;MD60NUDPxE1? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
Hep3B MX7D[YxtKEO7Y3zlJGF{e2G7 M3zZdVAvOS9yLkWvNk42KM7:TR?= MmfmOFghcA>? NIPsU4JFVVOR NW\tO2JzdGWjZIOgeI8h[SC|dXLzeIFvfGmjbDDhZ4N2dXWuYYTpc44hd2ZiSFPDJINmdGy|IHnuJJN2[i2JMTDwbIF{\cLi NHz1cVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HCCLM3 NX[yXWR3S2WubDDDfYNt\SCDc4PhfS=> NF;peGkxNjFxMD61M|IvPSEQvF2= NGSyc241QCCq NFvQNYJFVVOR NWXMcHpXdGWjZIOgeI8h[SC|dXLzeIFvfGmjbDDhZ4N2dXWuYYTpc44hd2ZiSFPDJINmdGy|IHnuJJN2[i2JMTDwbIF{\cLi NIfV[4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
Hep3B NGG0Z|JCeG:ydH;zbZMhSXO|YYm= M1jk[FAvOS9yLkWvNk42KM7:TR?= Ml\COFghcA>? NF20Z5ZFVVOR NHjDWHZi[3SrdnH0[ZMh[2G|cHHz[U0{KGGwZDDjZZNx[XOnLUmg[ZhxemW|c3nvckBidmRiaX7keYNm\CCSQWLQJINt\WG4YXflJIF{KHenbHygZZMh[3m2b3Podo9u\SClIILlcIVie2ViaX70c{B1cGViY4n0c5Bt[XOvIH\yc40hdWm2b3Poc45lemmj NVrZ[mZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HCCLM3 MnTmRZBweHSxc3nzJGF{e2G7 NIm2TZYxNjFxMD61M|IvPSEQvF2= NX3ne|RHPDhiaB?= MXrEUXNQ MXnhZ5RqfmG2ZYOgZ4F{eGG|ZT2zJIFv\CClYYPwZZNmNTliZYjwdoV{e2mxbjDhcoQhcW6mdXPl[EBRSVKSIHPs[YF3[WenIHHzJJdmdGxiYYOgZ5l1d2Oqcn;t[UBkKHKnbHXhd4UhcW62bzD0bIUh[3m2b4DsZZNuKG[{b32gcYl1d2Oqb37kdoli MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
A549 Ml7QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;XNE4yNTFyIN88US=> MmfZO|IhcA>? NVjBUXk6cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NEDsR|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
H157 M4DiUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYexdYZUOC5zLUGwJO69VQ>? MVW3NkBp NGjvN2ZqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MnzmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H1299 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLrNIhPOC5zLUGwJO69VQ>? NWHBT3hpPzJiaB?= MWHpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
A549 NIHWWZZHfW6ldHnvckBCe3OjeR?= Mke4NU8zNjVxNTFOwG0> MlfYNVIhcA>? M3nm[uKhf2Wja3z5JIRm[3KnYYPl[EBD[2xvMjDs[ZZmdHN? NILsco49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
H1299 MYXGeY5kfGmxbjDBd5NigQ>? NXjPdphrOS9{LkWvOUDPxE1? M2ezbVEzKGh? NGXCUHbDqHenYXvsfUBl\WO{ZXHz[YQhSmOuLUKgcIV3\Wy| M{\KOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H157 MVPGeY5kfGmxbjDBd5NigQ>? MoDONU8zNjVxNTFOwG0> MmnWNVIhcA>? MmT4[IVkemWjc3XkJGRTPCCneIDy[ZN{cW:w NWWxPWNuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 MUTGeY5kfGmxbjDBd5NigQ>? NWXU[VBjOS9{LkWvOUDPxE1? NXrhfldqOTJiaB?= NULwS3Qx\GWlcnXhd4VlKESUNDDlfJBz\XO|aX;u MmK3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
C8161 M2ToZWNmdGxiVnnhZoltcXS7IFHzd4F6 M376dVAuOiEQvF2= NV\HN4NXPCCm NF;o[ItFVVOR M3XkboRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M1\OW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel285 Mlm5R4VtdCCYaXHibYxqfHliQYPzZZk> M3;NcFAuOiEQvF2= M2\VfFQh\A>? NYPCO4wxTE2VTx?= MUnk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M2D5XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel290 NFj1[IRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1zqU|AuOiEQvF2= M4TENVQh\A>? NFfEdYxFVVOR Ml7N[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MmLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
92.1 M2XMcWNmdGxiVnnhZoltcXS7IFHzd4F6 NHewNWUxNTJizszN M1LycVQh\A>? M4rBTGROW09? NVjXT3ZS\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NX[xbZg4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1.3 NYDiSGRmS2WubDDWbYFjcWyrdImgRZN{[Xl? MlK4NE0zKM7:TR?= M1TTZ|Qh\A>? MnXQSG1UVw>? NXHoVm85\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MmezQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel202 NH6weZhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MWewMVIh|ryP NVnh[ZNGPCCm M2Hte2ROW09? MoHp[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NVPlZnZ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Mel270 MoPUR4VtdCCYaXHibYxqfHliQYPzZZk> MXOwMVIh|ryP NWXLTZgyPCCm MX7EUXNQ Mmew[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MofYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Omm1 M3TsWGNmdGxiVnnhZoltcXS7IFHzd4F6 NYrHOHg4OC1{IN88US=> MXS0JIQ> MkSySG1UVw>? NHvTWpFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M{XM[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
92.1 M4TwO2Fxd3C2b4Ppd{BCe3OjeR?= MljqOVAxKG6P MU[0PEBp NITieXZFVVOR MUHpcoR2[2W|IHHwc5B1d3Orcx?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Omm1.3 NHTv[5JCeG:ydH;zbZMhSXO|YYm= MnnGOVAxKG6P NFjJWFE1QCCq NEn5UmRFVVOR MkP6bY5lfWOnczDhdI9xfG:|aYO= NEX1bpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
92.1 MVLD[YxtKEO7Y3zlJGF{e2G7 NWDTcFlpPTByIH7N Ml2wNlQwPDhxN{KgbC=> MlzpSG1UVw>? NWnBNZNWcW6mdXPld{B1cGViY3XscEBi[2O3bYXsZZRqd25iYYSgd5VjNUdzwrC= NUjSR2hQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1.3 NVztcY5XS2WubDDDfYNt\SCDc4PhfS=> MkTROVAxKG6P MXeyOE81QC95MjDo NXHldZZxTE2VTx?= MkW2bY5lfWOnczD0bIUh[2WubDDhZ4N2dXWuYYTpc44h[XRic4XiMWcyyqB? MmTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
A549 M2LJZWZ2dmO2aX;uJGF{e2G7 MofyNVAxNzRyMD:xNFAxKG6P Mo\mNlQhcA>? Mk[0eZBz\We3bHH0[ZMh[W6mIHHjeIl3[XSnczDTTXJVOQ>? NVnUTY4xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yNVIyQTlpPkK2NlEzOTl7PD;hQi=>
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Rosetta2 DE3 NE[0UpNHfW6ldHnvckBie3OjeR?= NFjFRZRM\CB;IECuNFEyPyEQvF2= M1;NelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
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MV4-11 M3rzTGN6fG:2b4jpZ4l1gSCjc4PhfS=> M{e2dlQh\GG7cx?= M4[1eGlEPTBiPTCwMlAzPCEQvF2= NFvMZ3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
MV4-11 NFXTc2pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVK0JIRigXN? MUHJR|UxKD1iMD6wNlQh|ryP NV\XSFBCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NVroXIx[TnWwY4Tpc44h[XO|YYm= NUHQNnFmOzBibXnudy=> MXrJR|UxKD1iMD6wNlg4KM7:TR?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
NALM16 M4fyTGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGX2WmI2KGSjeYO= MoX3SWM2OCB;IECuNFMh|ryP M2jvS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUewNFI1Lz5{OUG3NFAzPDxxYU6=
BL21(DE3)-R3-pRARE2 NHPBcXhHfW6ldHnvckBie3OjeR?= M3;IPVMxKG2rboO= MY\JR|UxKD1iMD6wN|Mh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF7NUeyN{c,OjhzOUW3NlM9N2F-
BL21(DE3) MmjlSpVv[3Srb36gZZN{[Xl? M{C0SmtlKD1iMD6wN|Qh|ryP MkTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
Rosetta2 DE3 M{LWRmZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? MWmzNEBucW6| NE\ubmRKSzVyIE2gNE4xOzV5IN88US=> MoHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 MXXGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 NWHYem5TOzBibXnudy=> NGWwUI9KSzVyIE2gNE4xPDJ{IN88US=> NGDsNXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
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MOLM13 NVP6TlF3S3m2b4TvfIlkcXS7IHHzd4F6 M{i4V|Qh\GG7cx?= M2qxeWlEPTBiPTCwMlA2PiEQvF2= NV3w[GdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
MOLM13 NIjn[JVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnLoOEBl[Xm| NVr4WYtzUUN3MDC9JFAvODV4IN88US=> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
HL60 NE[zO5ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mk\hO|IhcA>? M4SyeGlEPTBiPTCwMlA3KM7:TR?= NX7QOnNVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxO|AxOjRpPkK5NVcxODJ2PD;hQi=>
HL60 NED3OodIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NG\OVVA{KGSjeYO= M{e0VmdEPTBiPTCwMlA3KM7:TR?= M{nWdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkW3NFk6Lz5{OU[1O|A6QTxxYU6=
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Raji M{DvPWZ2dmO2aX;uJIF{e2G7 NIHqWnZKSzVyIE2gNE4xPiEQvF2= M1e3RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
Raji MYfGeY5kfGmxbjDhd5NigQ>? MkHhOEBp NUjPeIg4UUN3MDC9JFAvODZ7IN88US=> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEe1PEc,OjR7MEC3OVg9N2F-
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BL21 (DE3)-codon plus-RIL NXz6d4dJTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= M3XycoJ6KG[udX;y[ZNk\W6lZTDhcol{d3S{b4D5JIF{e2G7 Mkj1TWM2OCB;IECuNFch|ryP NGfXe5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW4OlcyQCd-Mki1PFY4OTh:L3G+
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22Rv1 MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M4LiZ|EzKGh? M1mxN2lEPTBiPTCwMlA4OSEQvF2= NVfrU41rRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
MV4-11 MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NV7GXGFQUUN3MDC9JFAvODd{IN88US=> M37STlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUm1O|I{Lz5{OEG5OVczOzxxYU6=
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BL21(DE3)-R3-pRARE2 NGHRO4tHfW6ldHnvckBie3OjeR?= MXGzNEBucW6| MVTJR|UxKD1iMD6wO|ch|ryP M2PMZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUm1O|I{Lz5{OEG5OVczOzxxYU6=
BL21(DE3)-R3-pRARE2 NEW4[GpHfW6ldHnvckBie3OjeR?= M1K4O|MxKG2rboO= MV;JR|UxKD1iMD6wO|ch|ryP NEjkSmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe3Olg{PCd-Mkm3O|Y5OzR:L3G+
MV4-11 M{fBe2N6fG:2b4jpZ4l1gSCjc4PhfS=> M{X2cVI1KGh? NVjncYpjT0l3MDC9JFAvODhizszN MmHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzOUGzOlMoRjJ4MUmxN|Y{RC:jPh?=
MV411 NX;mUGZiSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MorLO|IhcA>? NETVcZVKSzVyIE2gNE4xQCEQvF2= NHTWRpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
TY82 NFH4RYlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MkjXO|IhcA>? M1T4bGlEPTBiPTCwMlA5ODhizszN MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV{NUSzOUc,Ojl3MkW0N|U9N2F-
HL60 M1\mUWZ2dmO2aX;uJIF{e2G7 MYCyOEBp MW\JR|UxKD1iMD6wPFYh|ryP NHnWcHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+
697 NVzyeZVPS3m2b4TvfIlkcXS7IHHzd4F6 MVy1JIRigXN? MmH5SWM2OCB;IECuNFkh|ryP Mn7FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
Loucy M4D5XWN6fG:2b4jpZ4l1gSCjc4PhfS=> NIjJdXM2KGSjeYO= M2XlO2VEPTBiPTCwMlA6KM7:TR?= NHTwS2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
BL21(DE3) MmjwSpVv[3Srb36gZZN{[Xl? NWLuPYt3U2RiPTCwMlA6OiEQvF2= NVTmdVVzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
BL21(DE3)-R3-pRARE2 M3vZbmZ2dmO2aX;uJIF{e2G7 MYjL[EA:KDBwMTFOwG0> NY\KUnY3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1PVUxODdpPkK4OVk2ODB5PD;hQi=>
HT-29 NWDjdpNHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYnXfIF4PzJiaB?= NEfKWWJKSzVyIE2gNE4yODRizszN MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3OUSyPEc,OjV3NUm0Nlg9N2F-
MM1S NYC3dJp3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHPjeFU4OiCq M2niWWlEPTBiPTCwMlExQSEQvF2= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3OUSyPEc,OjV3NUm0Nlg9N2F-
LNCAP cells M4LIS2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MXrJR|UxKD1iMD6xNFk3KM7:TR?= MlvJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
T cells M2XXU2Z2dmO2aX;uJIF{e2G7 MlHjNlQhcA>? MnvnTWM2OCB;IECuNVEh|ryP MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDNzNEWxN{c,Ojh|MUS1NVM9N2F-
HL60 NF7ub2NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NEfVR244OiCq NWPRUI1DUUN3MDC9JFAvOTFizszN MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh4OUG5OEc,OjZ6NkmxPVQ9N2F-
BL21(DE3) MmDDSpVv[3Srb36gZZN{[Xl? NEHIeZZKSzVyIE2gNE4yOiEQvF2= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
BL21(DE3) M{jLSmZ2dmO2aX;uJIF{e2G7 NHTCSIEzNjViaB?= M1L2[mlEPTBiPTCwMlEzKM7:TR?= NHXueWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
H1299 M2D3c2Z2dmO2aX;uJIF{e2G7 M4q1S|I1KGh? NUTZZ5JHTUN3MDC9JFAvOTV|IN88US=> M4DKfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUS5OVIyLz5{OEm0PVUzOTxxYU6=
HD-MB03 M36zd2N6fG:2b4jpZ4l1gSCjc4PhfS=> M{L4dFUh\GG7cx?= Mnz3SWM2OCB;IECuNVYh|ryP NVHCZ3I{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
LNCAP NUDpeYRySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MmnuPVYhcA>? NYXnSnI4UUN3MDC9JFAvOTZizszN MlvFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
Hs578T NEfybVBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3[1VFEzKGh? NIOwV4lKSzVyIE2gNE4yPiEQvF2= NYnhenNLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MV4-11 NUnYdGhHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MWqxNkBp NYDxNYFjUUN3MDC9JFAvOTZizszN MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
LNCAP NY[wOHRxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NIfoNHAyOiCq M4DHd2lEPTBiPTCwMlE3KM7:TR?= NEHUeGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
C4-2B NHTORYZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NVPnUox{QTZiaB?= M4rBZmlEPTBiPTCwMlE6KM7:TR?= M2LJNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
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Vero E6 MmLERY51cX[rcnHsJIFkfGm4aYT5JIF{e2G7 NHHw[WU1QCCq MVzJR|UxKD1iMD6xPVI4PSEQvF2= NGHnd4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MkO1N|g2QSd-M{KzOVM5PTl:L3G+
MCF7 NVTsSY1ZSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M4jNe|EzKGh? NV;KbodMUUN3MDC9JFAvOiEQvF2= MonWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MV4-11 NHjQVoZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MnjpTWM2OCB;IECuNlQh|ryP M4rFcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUSyO|UyLz5{N{G0Nlc2OTxxYU6=
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Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax ; c-Myc ; p27 26119999 23792448
Growth inhibition assay Cell viability 23792448
Immunofluorescence GM130 ; MHC / EdU 29074567 28733670
In vivo (+)-JQ1 (50 mg/kg) inhibits tumors growth in mice with NMC 797 xenografts. (+)-JQ1 (50 mg/kg) results in effacement of NUT nuclear speckles in mice with NMC 797 xenografts, consistent with competitive binding to nuclear chromatin. (+)-JQ1 (50 mg/kg) induces strong (grade 31) keratin expression in NMC 797 xenografts. (+)-JQ1 (50 mg/kg) promotes differentiation, tumor regression and prolonged survival in mice models of NMC xenografts. [1] (+)-JQ1 (50 mg/kg) results in a significant prolongation in overall survival of SCID-beige mice orthotopically xenografted after intravenous injection with MM.1S-luc+ cells compared to vehicle-treated animals. [2] (+)-JQ1 (50 mg/kg i.p.) leads to a highly significant increase in survival of mice bearing Raji xenografts. [3]

Protocol (from reference)

Cell Research:

[1]
  • Cell lines: MC 11060 cells
  • Concentrations: ~500 nM
  • Incubation Time: 48 hours
  • Method:

    Cells are seeded into white, 384-well microtiter plates at 500 cells per well in a total volume of 50 μL media. The 797, TT and TE10 cells are grown in DMEM containing 1% penicillin/streptomycin and 10% FBS. The Per403 cells are grown in DMEM containing 1 % penicillin/streptomycin and 20% FBS. Patient-derived NMC 11060 cells are grown in RPMI with 10% FBS and 1% penicillin/streptomycin. (+)-JQ1 is delivered to microtiter assay plates by robotic pin transfer. Following a 48 hours incubation at 37℃, cells are lysed and wells are assessed for total ATP content using a commercial proliferation assay. Replicate measurements are analyzed with respect to dose and estimates of IC50 are calculated by logistic regression (GraphPad Prism).
Animal Research:

[1]
  • Animal Models: Mice bearing NMC 797 xenografts
  • Dosages: 50 mg/kg
  • Administration: intraperitoneal injection

Solubility (25°C)

In vitro

 DMSO 91 mg/mL warmed
(199.12 mM)
Water Insoluble
Ethanol '91 mg/mL

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

 5mg/mL

Chemical Information

Molecular Weight 456.99
Formula

C23H25ClN4O2S
CAS No. 1268524-70-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(SC2=C1C(=NC(C3=NN=C(N32)C)CC(=O)OC(C)(C)C)C4=CC=C(C=C4)Cl)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How can I reconstitute the compound for in vivo injection?

Answer:
JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

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