PF-4708671

For research use only.

Licensed and Manufactured by Pfizer Catalog No.S2163

33 publications

PF-4708671 Chemical Structure

CAS No. 1255517-76-0

PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) with Ki/IC50 of 20 nM/160 nM in cell-free assays, 400-fold greater selectivity for S6K1 than S6K2, and 4- and >20-fold selectivity for S6K1 than MSK1 and RSK1/2, respectively. PF-4708671 induces autophagy. First S6K1-specific inhibitor to be reported.

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Selleck's PF-4708671 has been cited by 33 publications

Purity & Quality Control

Choose Selective S6 Kinase Inhibitors

Biological Activity

Description PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) with Ki/IC50 of 20 nM/160 nM in cell-free assays, 400-fold greater selectivity for S6K1 than S6K2, and 4- and >20-fold selectivity for S6K1 than MSK1 and RSK1/2, respectively. PF-4708671 induces autophagy. First S6K1-specific inhibitor to be reported.
Targets
p70 S6K1 [1]
(Cell-free assay)
160 nM
In vitro

PF-4708671 is a piperazinyl-pyrimidine analogue and the first S6K1-specific inhibitor. PF-4708671 does not significantly inhibit the activity of the closely related S6K2 isoform or a number of other AGC kinases (Akt1, Akt2, PKA, PKCα, PKCϵ, PRK2, ROCK2, RSK1, RSK2 or SGK1) . PF-4708671 prevents the S6K1-mediated phosphorylation of S6 protein in response to IGF-1 (insulin-like growth factor 1), while having no effect upon the PMA-induced phosphorylation of substrates of the highly related RSK (p90 ribosomal S6 kinase) and MSK (mitogen- and stress-activated kinase) kinases. PF-4708671 is also found to induce phosphorylation of the T-loop and hydrophobic motif of S6K1, an effect that is dependent upon mTORC1 (mTOR complex 1). PF-4708671 does not affect activity of mTORC1. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BHT-101 MmnES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fNXWlEPTB;MD64NlAzPCEQvF2= M3fNbHNCVkeHUh?=
UACC-893 NH\mfoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDEdWR{UUN3ME21MlE6QDh4IN88US=> NWHTeYxqW0GQR1XS
A427 NFvlUHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXwU|hKSzVyPUWuO|I2PjdizszN M1vJdHNCVkeHUh?=
OCUB-M NHnwdGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDrTWM2OD14Lk[2NVAzKM7:TR?= MX3TRW5ITVJ?
SW684 M{G1RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3ruWmlEPTB;Nz61NlI3QCEQvF2= MVHTRW5ITVJ?
SK-LMS-1 MmLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnqPG92UUN3ME25MlAyOzB{IN88US=> MUnTRW5ITVJ?
CP50-MEL-B MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTTe5RKSzVyPUmuN|A1OTlizszN NF;tSZlUSU6JRWK=
NCI-H1651 NVKxWYVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TEPWlEPTB;MUCuNFI4PiEQvF2= MmDsV2FPT0WU
ABC-1 NXr2dY4zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGj0cFBKSzVyPUGwMlExOzdizszN M4\jcHNCVkeHUh?=
OE19 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHBclhKSzVyPUGwMlE4OzNizszN NYrySIpQW0GQR1XS
DSH1 NHnnbIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYe5ZYlzUUN3ME2xNE45QDd7IN88US=> NID0NFdUSU6JRWK=
SW13 M3XkSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTFzLkmzNVkh|ryP M2jLOnNCVkeHUh?=
MDA-MB-453 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HJPWlEPTB;MUKuNVYxOyEQvF2= MlTvV2FPT0WU
647-V MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTF{LkO1PFIh|ryP MVfTRW5ITVJ?
NCI-H2342 NEPVdGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPFNHVKSzVyPUGyMlM6QTVizszN M1P2R3NCVkeHUh?=
LB2241-RCC M{fGW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXQcnlrUUN3ME2xNk42Pzd7IN88US=> MknXV2FPT0WU
UACC-257 NEnv[lRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmixTWM2OD1zMz60OFU{KM7:TR?= NWTpW2htW0GQR1XS
NCI-H661 M4izU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HkUGlEPTB;MUOuOlQ1QSEQvF2= NInab5JUSU6JRWK=
FADU MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnUTWM2OD1zMz62PVkzKM7:TR?= M2HqbHNCVkeHUh?=
A2780 MlP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTF|LkiwOFEh|ryP M3TvR3NCVkeHUh?=
NCI-H1793 NEjNRnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTF2LkSwPFUh|ryP MX;TRW5ITVJ?
TE-8 NUC4e4dwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPHTWM2OD1zNT6zOFY2KM7:TR?= MonyV2FPT0WU
HGC-27 M3u2S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlTXTWM2OD1zNj6xOlQ1KM7:TR?= NUjmWW1bW0GQR1XS
MMAC-SF MlriS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDrTWM2OD1zNz6zO|I4KM7:TR?= NEDOc4ZUSU6JRWK=
Calu-6 NVi1cm5ST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTF6LkG1NVMh|ryP MkjYV2FPT0WU
HUTU-80 NGDBXoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFX5RpFKSzVyPUG4MlM6PTlizszN M{jLbXNCVkeHUh?=
SAS MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLMW217UUN3ME2xPE43PDV3IN88US=> MXLTRW5ITVJ?
IST-MES1 M1Hrc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vhZmlEPTB;MUiuOlUxQCEQvF2= NX:5UphLW0GQR1XS
C3A NUXPcXU2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\6WGF6UUN3ME2xPE44QTF7IN88US=> NYTFUHpxW0GQR1XS
ES3 NV;hTFVPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTF6LkizOlQh|ryP NHO0NVFUSU6JRWK=
HSC-2 NVrOPFdOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTVTWM2OD1zOT62OVQyKM7:TR?= MWTTRW5ITVJ?
T47D NV23[FFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3O3NmlEPTB;MkCuNVQ5PSEQvF2= NWn3T2Q1W0GQR1XS
EW-7 M2DV[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXGVHllUUN3ME2yNE45PTh6IN88US=> MonKV2FPT0WU
LN-405 M1PidGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrTTWM2OD1{MD64PVY2KM7:TR?= NX7hRZRVW0GQR1XS
U031 MkXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXScoc{UUN3ME2yNU41QDB{IN88US=> NXm2d5FPW0GQR1XS
A549 NVvZdXQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLBd5duUUN3ME2yNU45PDlzIN88US=> NUnNeo05W0GQR1XS
RMG-I M2[0bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\QTWM2OD1{Mj6yOlY5KM7:TR?= M37kZ3NCVkeHUh?=
BT-474 NULrVnczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDydWFKSzVyPUKyMlY2PTFizszN MYLTRW5ITVJ?
NB69 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLEcI5KSzVyPUKzMlE2OiEQvF2= MWHTRW5ITVJ?
NB7 MnfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rLT2lEPTB;MkSuNVg5KM7:TR?= MUnTRW5ITVJ?
HCC1569 MmTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TOfWlEPTB;MkSuOlEzPSEQvF2= MUnTRW5ITVJ?
MEL-JUSO M4raOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHlZoU2UUN3ME2yOE43QTB6IN88US=> MWjTRW5ITVJ?
MDA-MB-175-VII NWDHbFFQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmG2TWM2OD1{NT6yOVE4KM7:TR?= NEf0OFVUSU6JRWK=
HCC1419 MmTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmnyTWM2OD1{NT62NFA{KM7:TR?= M3X4ZnNCVkeHUh?=
Ca9-22 NVrnSIlRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPJXXozUUN3ME2yOU43PDN{IN88US=> M{Gze3NCVkeHUh?=
KGN Mo\wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfHUY5KSzVyPUK1Mlc2PDdizszN NH;lNFRUSU6JRWK=
LB373-MEL-D MmLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnIPVhiUUN3ME2yOk4yQTN6IN88US=> MVPTRW5ITVJ?
NCI-H1755 NVLqV2RYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rXW2lEPTB;Mk[uPFg1OSEQvF2= NHTyboFUSU6JRWK=
D-423MG NHrLXWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVKwWItOUUN3ME2yO{4xPzF3IN88US=> NYfUdJBPW0GQR1XS
DK-MG M3rlTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXi1XFFPUUN3ME2yPE44QDR4IN88US=> MVfTRW5ITVJ?
NCI-H1623 Mk\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTJ6LkizOFIh|ryP MmXSV2FPT0WU
KU-19-19 NE\OTXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEL4SllKSzVyPUK4Mlk6PSEQvF2= NFTnXVVUSU6JRWK=
C-33-A MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTJ7LkKxPVgh|ryP MlXBV2FPT0WU
SCC-4 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPuZmtwUUN3ME2zNE4{OzV2IN88US=> MVvTRW5ITVJ?
MG-63 M365d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzRVoJHUUN3ME2zNE42QTl2IN88US=> MkLiV2FPT0WU
LNCaP-Clone-FGC NFHsdYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfWZ|lwUUN3ME2zNU41QTJzIN88US=> NVHDeWhiW0GQR1XS
CAPAN-1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTNzLkWyPFYh|ryP NW\YfoxpW0GQR1XS
GI-ME-N MofFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTNzLkWzOVMh|ryP MkXLV2FPT0WU
RERF-LC-MS MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLyXHpxUUN3ME2zNk43PzR5IN88US=> MlP6V2FPT0WU
KLE M2DJW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TOXmlEPTB;M{OuOFM{PCEQvF2= M2\HSHNCVkeHUh?=
KNS-81-FD MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nhU2lEPTB;M{OuOVAxQCEQvF2= M4OwNnNCVkeHUh?=
DOK MnrkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTWW4hTUUN3ME2zOE43PjF{IN88US=> MmjGV2FPT0WU
ACHN M4PPb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLRO5ZKSzVyPUO1MlE2QDhizszN MV3TRW5ITVJ?
KYSE-520 NGTvPWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7PTWM2OD1|NT6yN|YyKM7:TR?= NXHKVI1yW0GQR1XS
SBC-5 NXTxTmRNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;EXmhKSzVyPUO1MlUyOTZizszN MlHhV2FPT0WU
NCI-H28 NEnVZ5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTN3Lk[2PVUh|ryP NXHhe|BzW0GQR1XS
ES7 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfacnRKSzVyPUO2MlIzPDlizszN MkjHV2FPT0WU
A172 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTN4LkSxPFch|ryP Mmn3V2FPT0WU
UM-UC-3 NWH2TlByT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTN5Lki3OFYh|ryP MYnTRW5ITVJ?
LXF-289 MoroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;lTWM2OD1|OD6wNFQ4KM7:TR?= NFHP[JhUSU6JRWK=
KP-4 M2DORWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{L5fmlEPTB;M{iuOFc{QSEQvF2= NFzFb3hUSU6JRWK=
TE-1 M3\Nfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTKb5ZKSzVyPUO5MlY2QDlizszN MV;TRW5ITVJ?
G-402 M2DEeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnFTWM2OD12MD6wOlEh|ryP MYHTRW5ITVJ?
786-0 NEP0OWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHhdolHUUN3ME20NE46ODl4IN88US=> NILzXYlUSU6JRWK=
K5 M{KxSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrZUXRKSzVyPUSxMlM3QDZizszN NEnHPYxUSU6JRWK=
SK-N-DZ NX7sfGlPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3mTWM2OD12MT6zPFI{KM7:TR?= NWjNdlVSW0GQR1XS
OVCAR-4 MnXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnpUJNDUUN3ME20NU44PTZ6IN88US=> MoPtV2FPT0WU
T84 NGS0SZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTR{LkGyOlMh|ryP NIiyWW1USU6JRWK=
GCIY M{\tXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLZWItqUUN3ME20N{4xQDB|IN88US=> NVSxc3JtW0GQR1XS
BB49-HNC NFfCV|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vidGlEPTB;NEOuN|M1QCEQvF2= MV\TRW5ITVJ?
SNU-C2B MkHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTR|Lk[0N|Yh|ryP NYDNRVZtW0GQR1XS
TE-6 NEfxO|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTR|Lki4PVQh|ryP MlmzV2FPT0WU
23132-87 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7jTWM2OD12ND6yPVc5KM7:TR?= M4P2[XNCVkeHUh?=
DMS-53 NH\QXXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYi3cZhyUUN3ME20OE4{Ozh2IN88US=> NFv0bFhUSU6JRWK=
ONS-76 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLkS4FKSzVyPUS0Mlc3PjFizszN MUPTRW5ITVJ?
DMS-273 NXjKO3kzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\W[I9KSzVyPUS0Mlk1OyEQvF2= Mn[3V2FPT0WU
COLO-824 M1P1Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVi1fI9tUUN3ME20OU45Pzd3IN88US=> MUnTRW5ITVJ?
DMS-114 NHe3RWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLQXWk1UUN3ME20OU45QTJ5IN88US=> NGrKe21USU6JRWK=
YKG-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTR4LkCzOVUh|ryP M2LGS3NCVkeHUh?=
RH-18 NFXldZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTR4LkO3N|Mh|ryP MX\TRW5ITVJ?
NCI-H1355 NI\MU21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHnTXpKSzVyPUS2MlYyQCEQvF2= NWjzZVE2W0GQR1XS
NCI-H2170 Mnn2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVO2NGhvUUN3ME20O{44PTh2IN88US=> Ml7YV2FPT0WU
OC-314 NUXWcYR{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLlTWM2OD12Nz64OFc5KM7:TR?= Mn7TV2FPT0WU
NCI-H2030 NWjXTWhnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LYSmlEPTB;NEeuPFkxPiEQvF2= NIPBS|VUSU6JRWK=
22RV1 MlnNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvrO5BKSzVyPUS3MlkxOTVizszN M4rKUXNCVkeHUh?=
G-401 M3zLWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLMTWM2OD12OD60OFE2KM7:TR?= MmrnV2FPT0WU
YAPC MkfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml:xTWM2OD12OT64NFc{KM7:TR?= MmnKV2FPT0WU
SW1573 Ml70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\PNmNTUUN3ME21NE4zPDN3IN88US=> NHHGdGtUSU6JRWK=
COLO-792 NETnWINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzJOFBKSzVyPUWwMlYzOzdizszN NEHsW41USU6JRWK=
KNS-62 NF;MUmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTVyLke1NFgh|ryP M4PRR3NCVkeHUh?=
NB5 MlTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWLsRo53UUN3ME21NU41PTB7IN88US=> NUK2XIlMW0GQR1XS
CP66-MEL Mnu0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3sbpJbUUN3ME21NU43PDB6IN88US=> MmWxV2FPT0WU
CaR-1 NV7JWlk5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTV{LkC2NFYh|ryP NUnve|ZLW0GQR1XS
CAL-54 MnvaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTV{LkGxNFIh|ryP NFTpdI1USU6JRWK=
8305C M3n2T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTV{LkOxOUDPxE1? MUDTRW5ITVJ?
Calu-3 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkH6TWM2OD13Mj6zO|M{KM7:TR?= MYrTRW5ITVJ?
AU565 NIXhW3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTV{LkW1NFkh|ryP MXfTRW5ITVJ?
CW-2 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIWyfm9KSzVyPUWyMlg3PTRizszN MVvTRW5ITVJ?
BEN NFH5VXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjje4dFUUN3ME21N{44QDZ{IN88US=> M1L3UHNCVkeHUh?=
NCI-H1975 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWj3TIpIUUN3ME21N{45QTZ4IN88US=> M{LNWXNCVkeHUh?=
HCT-116 M363Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3\pUmlEPTB;NUSuNFMzPyEQvF2= MofhV2FPT0WU
SH-4 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LHb2lEPTB;NUSuNVM5KM7:TR?= NX3JRphIW0GQR1XS
NCI-H1693 NULkVXJLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjWS2dKSzVyPUW0MlIyODNizszN MYXTRW5ITVJ?
BPH-1 Mn;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13MPGlEPTB;NUWuNlg6OiEQvF2= NWOxNVAxW0GQR1XS
CAMA-1 NFq3Sm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;BOmlEPTB;NUWuPFEyPiEQvF2= MUjTRW5ITVJ?
A388 NETEPFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDid2lCUUN3ME21Ok4xQTh3IN88US=> NIHRd2dUSU6JRWK=
C2BBe1 M2H5e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\mbHZKSzVyPUW2MlI3OzRizszN NWLh[m5UW0GQR1XS
GAMG MnTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\BeGlEPTB;NU[uOVk2PSEQvF2= MlrmV2FPT0WU
MKN28 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmC5TWM2OD13Nj64NFE{KM7:TR?= NFfKSllUSU6JRWK=
VA-ES-BJ MkPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVL5OmRUUUN3ME21Ok45PzR3IN88US=> NXL6S3luW0GQR1XS
HuCCT1 NUHncJhkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\nZ|h5UUN3ME21O{4xPDl{IN88US=> MYXTRW5ITVJ?
BFTC-905 MnzXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHuTWM2OD13Nz6yPFk5KM7:TR?= MoXyV2FPT0WU
LS-123 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvKTWM2OD13Nz61OlY5KM7:TR?= NHLnTGlUSU6JRWK=
LB996-RCC MkjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nsbWlEPTB;NUiuNFI5PyEQvF2= M4KxbXNCVkeHUh?=
NCI-H1048 MmHxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojTTWM2OD13OD6yNVg5KM7:TR?= NWHWTWRQW0GQR1XS
COR-L105 NILuSGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLUTWM2OD13OD63N|M4KM7:TR?= MXXTRW5ITVJ?
OVCAR-8 M{juemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHpdo1kUUN3ME21PU4yPzF4IN88US=> NGq0RohUSU6JRWK=
SK-N-FI MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fPUWlEPTB;NUmuN|IxPyEQvF2= MYnTRW5ITVJ?
MES-SA M3rtT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrKTWM2OD13OT60OVA6KM7:TR?= NYjxUGx7W0GQR1XS
SCC-9 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH3JSHpKSzVyPUW5MlQ4PTNizszN M{nXdnNCVkeHUh?=
EFO-27 NFjQU2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDPSJNKSzVyPUW5Mlk1OyEQvF2= MkfiV2FPT0WU
GT3TKB MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTZyLk[wOFgh|ryP NIG4TVFUSU6JRWK=
HCC38 NHviNYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTZzLkSzO|kh|ryP NVHMb5hiW0GQR1XS
MLMA MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrR[4FSUUN3ME22NU43OTZ|IN88US=> M3LRZXNCVkeHUh?=
D-566MG MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nyTmlEPTB;NkOuOVIzPCEQvF2= MX7TRW5ITVJ?
SF295 M1;YXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPZfpBGUUN3ME22N{43PjV3IN88US=> M1KxVXNCVkeHUh?=
ES4 MoXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLCc25KSzVyPU[0MlQ4OTlizszN MYLTRW5ITVJ?
CAKI-1 MoLHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDvdXlqUUN3ME22OE42PzJzIN88US=> NF3HdZNUSU6JRWK=
OAW-28 NEnkO4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTZ2Lkm2OFch|ryP MnLxV2FPT0WU
NCI-H460 MmP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnMS5IxUUN3ME22OU4xQDl5IN88US=> NVvzUFNRW0GQR1XS
JAR M4Lafmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XNSmlEPTB;NkWuOVUyPiEQvF2= M2\TWnNCVkeHUh?=
SNU-449 NI\nXnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrxeXl7UUN3ME22OU44PjF4IN88US=> MVjTRW5ITVJ?
D-392MG NWThSVA2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHCZllKSzVyPU[1Mlk1OzRizszN NVXGV3lCW0GQR1XS
EW-22 NULPe2ZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTZ4LkO5OUDPxE1? MmjrV2FPT0WU
GCT M2DoO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfUeZpoUUN3ME22O{4xPDR|IN88US=> NHnB[VVUSU6JRWK=
NOS-1 Moj6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTZ5Lk[4Olch|ryP NH\JT5ZUSU6JRWK=
RH-1 NY[1ZXlYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jmdmlEPTB;NkiuOVU2QSEQvF2= MYrTRW5ITVJ?
HuH-7 NG\HdWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTZ7LkGwNFIh|ryP MVTTRW5ITVJ?
NUGC-3 MlWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1O3XGlEPTB;N{CuPVY1PCEQvF2= NHfHbW9USU6JRWK=
IST-SL1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULXXm1jUUN3ME23NU4zOzJ2IN88US=> M3HkfnNCVkeHUh?=
SHP-77 M3uxcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVn6[XlOUUN3ME23Nk4yOTlizszN NGO3OZFUSU6JRWK=
SW1116 NVjnS5NQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfNTWM2OD15Mj6xOFA4KM7:TR?= NIq3dm5USU6JRWK=
LK-2 NWraXJZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXsTWM2OD15Mj64OlEzKM7:TR?= MYHTRW5ITVJ?
U-2-OS NXPad4pYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWO4UVlQUUN3ME23N{4yQDJ6IN88US=> MX;TRW5ITVJ?
KYSE-150 MkLlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTd|Lk[xNVgh|ryP NXXYPGw6W0GQR1XS
U251 NYXa[ZBST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3qydGlEPTB;N{OuPFE3QCEQvF2= NEPqWphUSU6JRWK=
HT-1080 M4HBbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XoS2lEPTB;N{SuNlU4PCEQvF2= NIHie|VUSU6JRWK=
NCI-H1437 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTd2Lk[zNlYh|ryP MkDOV2FPT0WU
HPAF-II MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV[0blFZUUN3ME23OE43PDF3IN88US=> MX;TRW5ITVJ?
Becker NYLCO4E2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTd3LkGxNFUh|ryP MW\TRW5ITVJ?
PANC-10-05 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTd3LkK4OlUh|ryP NG\KcZlUSU6JRWK=
TYK-nu NEPZVJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTd3LkOxNFIh|ryP MWPTRW5ITVJ?
SKG-IIIa NIm4W5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\lVFBKSzVyPUe1MlM{PDFizszN MXjTRW5ITVJ?
NCI-H1993 NFHIWotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGizOIJKSzVyPUe1Mlc{QTdizszN NWDhVGVvW0GQR1XS
S-117 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTd3Lke1OUDPxE1? MmDXV2FPT0WU
HuO9 NWrnVnl{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDjTnU6UUN3ME23Ok4xODR6IN88US=> NEHjdnNUSU6JRWK=
CAL-51 MknSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXWZXdFUUN3ME23Ok4yODB4IN88US=> MVXTRW5ITVJ?
BT-20 M1PhZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILF[llKSzVyPUe2MlIyPjhizszN NVHYPJI6W0GQR1XS
LB831-BLC MnL5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTd4LkOyNVch|ryP Mn;GV2FPT0WU
LB1047-RCC NXrCR2tFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzkbph1UUN3ME23Ok44PjF7IN88US=> NID2cZpUSU6JRWK=
ES8 NUf5XmdxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTd6LkCwO|Mh|ryP NYTvVJRJW0GQR1XS
UMC-11 MoXmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTd6LkC4PFQh|ryP M4ewcXNCVkeHUh?=
NCI-H226 NEnyVJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTd6LkGzNVUh|ryP MlnwV2FPT0WU
IGROV-1 NVewXI5uT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkOyTWM2OD15OD61OVUh|ryP NGTLVYdUSU6JRWK=
BHY MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;hdGxKSzVyPUe5MlU6PiEQvF2= NVjrTHdCW0GQR1XS
MKN45 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDVPY9KSzVyPUiwMlMyPzRizszN MYnTRW5ITVJ?
IA-LM NVX0fmtCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LQXGlEPTB;OECuN|UyOSEQvF2= NFPLdFBUSU6JRWK=
WM-115 NXznT4dPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRThzLkSxNVQh|ryP MWrTRW5ITVJ?
VMRC-RCZ NIHq[YpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRThzLkS0NlUh|ryP NUi1dmdtW0GQR1XS
NCI-H2291 NXTEOHVPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PKTGlEPTB;OEGuOlg1OiEQvF2= MYPTRW5ITVJ?
MCF7 M3\teWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mof6TWM2OD16Mz6yPFk4KM7:TR?= M3\rRXNCVkeHUh?=
639-V MlG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3L3eGlEPTB;OESuOlEzQSEQvF2= Mmn4V2FPT0WU
RT4 NXjwcnczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjabY1KSzVyPUi1MlI1ODNizszN NYPsZ|R5W0GQR1XS
NCI-H2405 Mmi5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XMXmlEPTB;OEWuNlk6PyEQvF2= NIX6[XZUSU6JRWK=
CAL-33 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3K0SWlEPTB;OEWuOlMyPSEQvF2= M2PWdHNCVkeHUh?=
BT-549 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHRPWpKSzVyPUi1Mlk{QDJizszN M4jjdXNCVkeHUh?=
HT-1197 NGLWNlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUC0OHB2UUN3ME24PE4yODd5IN88US=> MorOV2FPT0WU
G-361 MoLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\LeGlEPTB;OEiuNVQ4PCEQvF2= MXrTRW5ITVJ?
LS-411N MmHzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTh7LkOzPFgh|ryP NF[0[4pUSU6JRWK=
HT-144 MnjTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzSTWM2OD16OT61PVkzKM7:TR?= M3XHeHNCVkeHUh?=
DJM-1 NVO2N4lpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTh7Lki4OFUh|ryP NXTsOZpOW0GQR1XS
CAL-12T MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFi4eY5KSzVyPUmwMlc5QTVizszN NH:3N5VUSU6JRWK=
MKN7 M3jGcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTlzLkO1OVEh|ryP M{W0cnNCVkeHUh?=
HuP-T3 Moj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvtR|RWUUN3ME25Ok4yODB2IN88US=> M4XZUHNCVkeHUh?=
SK-MG-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\I[mJFUUN3ME25Ok4yOzR6IN88US=> NYPpbWZSW0GQR1XS
NCI-H2347 NYnrcJczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHG2WXlKSzVyPUm4MlExPDJizszN NYjlPHhbW0GQR1XS
SW872 M1zLSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXnLT2tzUUN3ME2xNFAvPDV4IN88US=> MUnTRW5ITVJ?
COLO-829 M1PpfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETZ[3lKSzVyPUGwNE42PTNizszN M4\UfHNCVkeHUh?=
MC-IXC MoHGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;Ed2U6UUN3ME2xNFAvPjF3IN88US=> NILhOIJUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pS6 / S6 / pS6K / S6K1 / pAKT / AKT ; 

PubMed: 28626016     


Western blot of cell lysates from neurons treated with PF-4708671, rapamycin, and Torin-2, individually or in combination for the indicated times.

p-mTOR / mTOR / p-p70S6K / p70S6K ; 

PubMed: 30423811     


As determined using Western blotting, the phospho-p70S6K (p-p70S6K) inhibitor (PF-4708671) reduced the expression of phospho-mTOR (p-mTOR) and p-p70S6K in SCC-4 and SCC-25 cells. SCC-4 and SCC-25 cells were not treated as negative control (NC) or treated with 20 µM PF-4708671 for 6 h (6 h), 12 h (12 h), 18 h (18 h), 1 day (1 d), 2 days (2 d), and 3 days (3 d). Western blot analysis was performed to examine the expression levels of p-mTOR, mTOR, p-p70S6K, p70S6K, and β-actin.

28626016 30423811
Growth inhibition assay
Cell viability; 

PubMed: 30423811     


Inhibitory effects of various doses (0.5, 1, 2, 5, 10, 20, and 40 µM) of PF-4708671 on SCC-4 (B) and SCC-25 (C) cells were assessed using MTT assay after 72 h of treatment. Data presented are the mean and standard error of the mean of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate the statistical significance of differences between cells with and without treatment. t (red ** and *** for SCC-4 and blue *, ** and *** for SCC-25).

30423811

Protocol

Solubility (25°C)

In vitro DMSO 30 mg/mL (76.84 mM)
Ethanol 8 mg/mL (20.49 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 390.41
Formula

C19H21F3N6

CAS No. 1255517-76-0
Storage powder
in solvent
Synonyms N/A
Smiles CCC1=CN=CN=C1N2CCN(CC2)CC3=NC4=C(N3)C=C(C=C4)C(F)(F)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    Whether the in vivo formula "30% PEG400+0.5% Tween80+5% Propylene glycol" for S2163 is suitable for i.p. or not?

  • Answer:

    The formula "30% PEG400+0.5% Tween80+5% Propylene glycol" on our website is an oral one. It is a suspension.

S6 Kinase Signaling Pathway Map

Related S6 Kinase Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID