PF-4708671

For research use only.

Licensed and Manufactured by Pfizer Catalog No.S2163

25 publications

PF-4708671 Chemical Structure

CAS No. 1255517-76-0

PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) with Ki/IC50 of 20 nM/160 nM in cell-free assays, 400-fold greater selectivity for S6K1 than S6K2, and 4- and >20-fold selectivity for S6K1 than MSK1 and RSK1/2, respectively. PF-4708671 induces autophagy. First S6K1-specific inhibitor to be reported.

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Selleck's PF-4708671 has been cited by 25 publications

Purity & Quality Control

Choose Selective S6 Kinase Inhibitors

Biological Activity

Description PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) with Ki/IC50 of 20 nM/160 nM in cell-free assays, 400-fold greater selectivity for S6K1 than S6K2, and 4- and >20-fold selectivity for S6K1 than MSK1 and RSK1/2, respectively. PF-4708671 induces autophagy. First S6K1-specific inhibitor to be reported.
Targets
p70 S6K1 [1]
(Cell-free assay)
160 nM
In vitro

PF-4708671 is a piperazinyl-pyrimidine analogue and the first S6K1-specific inhibitor. PF-4708671 does not significantly inhibit the activity of the closely related S6K2 isoform or a number of other AGC kinases (Akt1, Akt2, PKA, PKCα, PKCϵ, PRK2, ROCK2, RSK1, RSK2 or SGK1) . PF-4708671 prevents the S6K1-mediated phosphorylation of S6 protein in response to IGF-1 (insulin-like growth factor 1), while having no effect upon the PMA-induced phosphorylation of substrates of the highly related RSK (p90 ribosomal S6 kinase) and MSK (mitogen- and stress-activated kinase) kinases. PF-4708671 is also found to induce phosphorylation of the T-loop and hydrophobic motif of S6K1, an effect that is dependent upon mTORC1 (mTOR complex 1). PF-4708671 does not affect activity of mTORC1. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BHT-101 MnnRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlP6TWM2OD1yLkiyNFI1KM7:TR?= MnnHV2FPT0WU
UACC-893 M{LxUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\MTWM2OD13LkG5PFg3KM7:TR?= MnfXV2FPT0WU
A427 NIXHeYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVO1Z5M4UUN3ME21MlczPTZ5IN88US=> NUPzOHRkW0GQR1XS
OCUB-M NUWxUHl3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTZwNk[xNFIh|ryP NXPKRYpkW0GQR1XS
SW684 MoqxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\3TWM2OD15LkWyNlY5KM7:TR?= NHTYW4FUSU6JRWK=
SK-LMS-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWe5boE5UUN3ME25MlAyOzB{IN88US=> MYXTRW5ITVJ?
CP50-MEL-B NVf5flBGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnMTWM2OD17LkOwOFE6KM7:TR?= M1\Pe3NCVkeHUh?=
NCI-H1651 MlTQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nse2lEPTB;MUCuNFI4PiEQvF2= M1zjTHNCVkeHUh?=
ABC-1 M4G0Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTBTWM2OD1zMD6xNFM4KM7:TR?= Mmn5V2FPT0WU
OE19 NWnyVVNOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXjJR|UxRTFyLkG3N|Mh|ryP NFnJTJFUSU6JRWK=
DSH1 NFrOUFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTFyLki4O|kh|ryP MmK1V2FPT0WU
SW13 NVL5NIVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDMem1tUUN3ME2xNU46OzF7IN88US=> MUfTRW5ITVJ?
MDA-MB-453 M1\J[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmH4TWM2OD1zMj6xOlA{KM7:TR?= NXnEUHF3W0GQR1XS
647-V M4HqVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkmyTWM2OD1zMj6zOVgzKM7:TR?= MVPTRW5ITVJ?
NCI-H2342 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\hTJhHUUN3ME2xNk4{QTl3IN88US=> M{\Jb3NCVkeHUh?=
LB2241-RCC Moi2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPBeVFKSzVyPUGyMlU4PzlizszN M37LcXNCVkeHUh?=
UACC-257 NUH2fWhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XxU2lEPTB;MUOuOFQ2OyEQvF2= NH\3[lRUSU6JRWK=
NCI-H661 NUn0WIp6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHLBVmNKSzVyPUGzMlY1PDlizszN MUjTRW5ITVJ?
FADU NUHWXZVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTF|Lk[5PVIh|ryP MnnwV2FPT0WU
A2780 MnjBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jFSmlEPTB;MUOuPFA1OSEQvF2= NVW4TVN{W0GQR1XS
NCI-H1793 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY[1RYxDUUN3ME2xOE41ODh3IN88US=> M{js[XNCVkeHUh?=
TE-8 NHSweHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rsNmlEPTB;MUWuN|Q3PSEQvF2= NW\VVXU1W0GQR1XS
HGC-27 NUXHN|hoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3G5OGlEPTB;MU[uNVY1PCEQvF2= NUXPeI9ZW0GQR1XS
MMAC-SF MmLlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTF5LkO3Nlch|ryP NVjLN3RVW0GQR1XS
Calu-6 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTF6LkG1NVMh|ryP NFvvdZNUSU6JRWK=
HUTU-80 NWOybZhOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTF6LkO5OVkh|ryP MUjTRW5ITVJ?
SAS Ml;NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzqVHBKSzVyPUG4MlY1PTVizszN NYrMN4pNW0GQR1XS
IST-MES1 M4\x[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGj2bWlKSzVyPUG4MlY2ODhizszN NE\ZR3VUSU6JRWK=
C3A MlzGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTF6Lke5NVkh|ryP M1jRTHNCVkeHUh?=
ES3 NFTSdHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXubFl5UUN3ME2xPE45OzZ2IN88US=> MVLTRW5ITVJ?
HSC-2 NHS2coRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfNUoFKSzVyPUG5MlY2PDFizszN MkTaV2FPT0WU
T47D MlfsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHMbYpKSzVyPUKwMlE1QDVizszN Mm\XV2FPT0WU
EW-7 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTJyLki1PFgh|ryP MmTuV2FPT0WU
LN-405 MnLVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7tVHRTUUN3ME2yNE45QTZ3IN88US=> M{HDZnNCVkeHUh?=
U031 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLuTWM2OD1{MT60PFAzKM7:TR?= M37yR3NCVkeHUh?=
A549 NI\FVYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HFRmlEPTB;MkGuPFQ6OSEQvF2= MlzvV2FPT0WU
RMG-I MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1KySWlEPTB;MkKuNlY3QCEQvF2= NIS4NotUSU6JRWK=
BT-474 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvmNow4UUN3ME2yNk43PTVzIN88US=> M3X2c3NCVkeHUh?=
NB69 Mo\oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHJSmdlUUN3ME2yN{4yPTJizszN Mn3qV2FPT0WU
NB7 M{jVVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vV[GlEPTB;MkSuNVg5KM7:TR?= NUXIcmRyW0GQR1XS
HCC1569 NXztT3pQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vMO2lEPTB;MkSuOlEzPSEQvF2= NY\HT|lWW0GQR1XS
MEL-JUSO MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTJ2Lk[5NFgh|ryP MVfTRW5ITVJ?
MDA-MB-175-VII NX32XYpWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vTbWlEPTB;MkWuNlUyPyEQvF2= NGnMbItUSU6JRWK=
HCC1419 NVP5W|d3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlfRTWM2OD1{NT62NFA{KM7:TR?= NX;Je5RmW0GQR1XS
Ca9-22 MkDFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPwTWM2OD1{NT62OFMzKM7:TR?= M1P1cHNCVkeHUh?=
KGN NEHtflhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2G1VGlEPTB;MkWuO|U1PyEQvF2= MkLZV2FPT0WU
LB373-MEL-D M2HFXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\ZcYhZUUN3ME2yOk4yQTN6IN88US=> NUHXOplpW0GQR1XS
NCI-H1755 MlfJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfjTWM2OD1{Nj64PFQyKM7:TR?= NIn1PHZUSU6JRWK=
D-423MG MorjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEj1[2FKSzVyPUK3MlA4OTVizszN NFvuWpVUSU6JRWK=
DK-MG MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHOXnRKSzVyPUK4Mlc5PDZizszN M2PSenNCVkeHUh?=
NCI-H1623 NX:1bIE{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTJ6LkizOFIh|ryP NYe2ZoM1W0GQR1XS
KU-19-19 NYKxbllwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXHb|ZKSzVyPUK4Mlk6PSEQvF2= NF7Qd3ZUSU6JRWK=
C-33-A MoTUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETINFBKSzVyPUK5MlIyQThizszN MVnTRW5ITVJ?
SCC-4 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3MTWM2OD1|MD6zN|U1KM7:TR?= NUD6S4NCW0GQR1XS
MG-63 NF7FdnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEeyeY5KSzVyPUOwMlU6QTRizszN NHHmeHVUSU6JRWK=
LNCaP-Clone-FGC NVTqbGlsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1X5dmlEPTB;M{GuOFkzOSEQvF2= NXzQSmd1W0GQR1XS
CAPAN-1 NXn5ToZ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXr6TZBwUUN3ME2zNU42Ojh4IN88US=> NXjNVZRiW0GQR1XS
GI-ME-N M2HZV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7iTWM2OD1|MT61N|U{KM7:TR?= MVvTRW5ITVJ?
RERF-LC-MS NI\v[2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTN{Lk[3OFch|ryP NWPZbZBPW0GQR1XS
KLE MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzJe2k2UUN3ME2zN{41OzN2IN88US=> NV3kO2JPW0GQR1XS
KNS-81-FD MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITkbIFKSzVyPUOzMlUxODhizszN NF[5PXRUSU6JRWK=
DOK NEDHZmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;STWM2OD1|ND62OlEzKM7:TR?= MlfKV2FPT0WU
ACHN M1[4Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzpWIZKSzVyPUO1MlE2QDhizszN MmLPV2FPT0WU
KYSE-520 M3HGXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3CWIJoUUN3ME2zOU4zOzZzIN88US=> NXvpVmh[W0GQR1XS
SBC-5 NUTTO4ROT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHT1T4FKSzVyPUO1MlUyOTZizszN NFTse|RUSU6JRWK=
NCI-H28 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTN3Lk[2PVUh|ryP Ml7uV2FPT0WU
ES7 M{fy[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDtSHRKSzVyPUO2MlIzPDlizszN M1vkdnNCVkeHUh?=
A172 MmPnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXnTWM2OD1|Nj60NVg4KM7:TR?= MVjTRW5ITVJ?
UM-UC-3 NWPRNVBQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV[zS2p4UUN3ME2zO{45PzR4IN88US=> MWPTRW5ITVJ?
LXF-289 M3HHdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3qT5JvUUN3ME2zPE4xODR5IN88US=> NE\aXXZUSU6JRWK=
KP-4 M3[4eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXewcFVPUUN3ME2zPE41PzN7IN88US=> MnzZV2FPT0WU
TE-1 NGXZRo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTQZZlIUUN3ME2zPU43PTh7IN88US=> MVjTRW5ITVJ?
G-402 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjZTWM2OD12MD6wOlEh|ryP MnfqV2FPT0WU
786-0 NUK1S3JOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTRyLkmwPVYh|ryP MlfWV2FPT0WU
K5 Mmi0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LxSmlEPTB;NEGuN|Y5PiEQvF2= NIjaTGhUSU6JRWK=
SK-N-DZ NWLVNHNsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXuTWM2OD12MT6zPFI{KM7:TR?= MUHTRW5ITVJ?
OVCAR-4 M{GxXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvCSXRKSzVyPUSxMlc2PjhizszN NVPsO2o2W0GQR1XS
T84 Ml21S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELufWdKSzVyPUSyMlEzPjNizszN NFm2VphUSU6JRWK=
GCIY MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rRPGlEPTB;NEOuNFgxOyEQvF2= NHO5R5JUSU6JRWK=
BB49-HNC NHnQ[3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWSxe4U2UUN3ME20N{4{OzR6IN88US=> NUfydGY{W0GQR1XS
SNU-C2B M4jqfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vhNmlEPTB;NEOuOlQ{PiEQvF2= MXrTRW5ITVJ?
TE-6 NGW0NGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTWXnNXUUN3ME20N{45QDl2IN88US=> NV3xbnpMW0GQR1XS
23132-87 NX[zU2hYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFftXYxKSzVyPUS0MlI6PzhizszN MX\TRW5ITVJ?
DMS-53 M13LV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXu1R2VkUUN3ME20OE4{Ozh2IN88US=> MknQV2FPT0WU
ONS-76 NVPYclQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUS5UGhqUUN3ME20OE44PjZzIN88US=> M3fqSXNCVkeHUh?=
DMS-273 MofXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTR2Lkm0N{DPxE1? NIDye4xUSU6JRWK=
COLO-824 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXy2V5JRUUN3ME20OU45Pzd3IN88US=> NHnmco5USU6JRWK=
DMS-114 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7BUGlKSzVyPUS1Mlg6OjdizszN M1fQPXNCVkeHUh?=
YKG-1 NHrWUmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\1UmlEPTB;NE[uNFM2PSEQvF2= NX25S|NVW0GQR1XS
RH-18 M3TmU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTR4LkO3N|Mh|ryP Mo\4V2FPT0WU
NCI-H1355 NIr6UW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVn0WmRCUUN3ME20Ok43OThizszN M1rofXNCVkeHUh?=
NCI-H2170 NYnNVmc5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;nXo0zUUN3ME20O{44PTh2IN88US=> MoLsV2FPT0WU
OC-314 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlz6TWM2OD12Nz64OFc5KM7:TR?= NIHjbWVUSU6JRWK=
NCI-H2030 M3XwR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zmNGlEPTB;NEeuPFkxPiEQvF2= MXHTRW5ITVJ?
22RV1 M{LDRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTR5LkmwNVUh|ryP NYK5VYtZW0GQR1XS
G-401 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7sUHZKSzVyPUS4MlQ1OTVizszN NUTnOlNOW0GQR1XS
YAPC MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTR7LkiwO|Mh|ryP M3PvWXNCVkeHUh?=
SW1573 NI\NN4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTVyLkK0N|Uh|ryP MlTBV2FPT0WU
COLO-792 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULU[|hOUUN3ME21NE43OjN5IN88US=> Moj3V2FPT0WU
KNS-62 MnTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HL[WlEPTB;NUCuO|UxQCEQvF2= MWfTRW5ITVJ?
NB5 NFHjbYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\0TWM2OD13MT60OVA6KM7:TR?= M3fybnNCVkeHUh?=
CP66-MEL NIjqSHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1i4TmlEPTB;NUGuOlQxQCEQvF2= MXTTRW5ITVJ?
CaR-1 NIjYUJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTV{LkC2NFYh|ryP M1TITHNCVkeHUh?=
CAL-54 M1PsdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zGTGlEPTB;NUKuNVExOiEQvF2= MkjpV2FPT0WU
8305C NXG0W|JKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\qTIJlUUN3ME21Nk4{OTVizszN M2LlenNCVkeHUh?=
Calu-3 NX;BXGdsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjNcHFVUUN3ME21Nk4{PzN|IN88US=> M4LUcXNCVkeHUh?=
AU565 Ml;ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\MWYlKSzVyPUWyMlU2ODlizszN MWPTRW5ITVJ?
CW-2 NH3sT29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\VTWM2OD13Mj64OlU1KM7:TR?= MXXTRW5ITVJ?
BEN NGPlWpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkf2TWM2OD13Mz63PFYzKM7:TR?= MojSV2FPT0WU
NCI-H1975 NGHGbXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY[1UVR7UUN3ME21N{45QTZ4IN88US=> Mmr3V2FPT0WU
HCT-116 NFzMPFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTV2LkCzNlch|ryP NXG1W4hNW0GQR1XS
SH-4 NUXUfW42T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7iTWM2OD13ND6xN|gh|ryP MWLTRW5ITVJ?
NCI-H1693 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIrwOVVKSzVyPUW0MlIyODNizszN MlW4V2FPT0WU
BPH-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rMOGlEPTB;NUWuNlg6OiEQvF2= MVHTRW5ITVJ?
CAMA-1 NWewU|MyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW[4W3hIUUN3ME21OU45OTF4IN88US=> NYPSd3ZHW0GQR1XS
A388 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\2TWM2OD13Nj6wPVg2KM7:TR?= MnTpV2FPT0WU
C2BBe1 NVXvNG16T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrNXoc1UUN3ME21Ok4zPjN2IN88US=> NYnRSpdlW0GQR1XS
GAMG NVXBeoJiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mly4TWM2OD13Nj61PVU2KM7:TR?= MmXyV2FPT0WU
MKN28 M1;KeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzDTYE5UUN3ME21Ok45ODF|IN88US=> M1flOHNCVkeHUh?=
VA-ES-BJ NHvqUWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTV4Lki3OFUh|ryP M{K4dnNCVkeHUh?=
HuCCT1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nTXWlEPTB;NUeuNFQ6OiEQvF2= MnjsV2FPT0WU
BFTC-905 M1;5cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLwVnpFUUN3ME21O{4zQDl6IN88US=> MXvTRW5ITVJ?
LS-123 MoPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTV5LkW2Olgh|ryP NF\IZ|ZUSU6JRWK=
LB996-RCC Mn7PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTV6LkCyPFch|ryP NUT4NYNyW0GQR1XS
NCI-H1048 M2rVdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXQb4lKSzVyPUW4MlIyQDhizszN NFe0eFdUSU6JRWK=
COR-L105 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXNSpRGUUN3ME21PE44OzN5IN88US=> MnHsV2FPT0WU
OVCAR-8 NGnsfXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTV7LkG3NVYh|ryP NH63XpRUSU6JRWK=
SK-N-FI MoSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLUTWM2OD13OT6zNlA4KM7:TR?= M1iwZ3NCVkeHUh?=
MES-SA MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHLSodKUUN3ME21PU41PTB7IN88US=> NF2yd5pUSU6JRWK=
SCC-9 MmLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnCTWM2OD13OT60O|U{KM7:TR?= MYjTRW5ITVJ?
EFO-27 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7KWVhjUUN3ME21PU46PDNizszN MkHWV2FPT0WU
GT3TKB NXznfFJJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTZyLk[wOFgh|ryP MorGV2FPT0WU
HCC38 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlziTWM2OD14MT60N|c6KM7:TR?= NYHpNZNFW0GQR1XS
MLMA M1LBTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfFTIJEUUN3ME22NU43OTZ|IN88US=> NV;RV2dMW0GQR1XS
D-566MG NX\V[WFtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LZUWlEPTB;NkOuOVIzPCEQvF2= NYfUcZF4W0GQR1XS
SF295 NITre5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorOTWM2OD14Mz62OlU2KM7:TR?= MWfTRW5ITVJ?
ES4 NXLFUIRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\wOmlEPTB;NkSuOFcyQSEQvF2= MmPQV2FPT0WU
CAKI-1 MnXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF[2fYdKSzVyPU[0MlU4OjFizszN M1Ky[nNCVkeHUh?=
OAW-28 NFrpelNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELzRZJKSzVyPU[0Mlk3PDdizszN MVLTRW5ITVJ?
NCI-H460 NWnIbVdKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTwTWM2OD14NT6wPFk4KM7:TR?= NXTLSm9uW0GQR1XS
JAR NFXxV5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTZ3LkW1NVYh|ryP NU[1boNKW0GQR1XS
SNU-449 NHz0Z5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\E[lN1UUN3ME22OU44PjF4IN88US=> Mn7xV2FPT0WU
D-392MG M1v3Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vvRmlEPTB;NkWuPVQ{PCEQvF2= NIL6NVdUSU6JRWK=
EW-22 NV;oToxZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknUTWM2OD14Nj6zPVUh|ryP MX3TRW5ITVJ?
GCT MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTZ5LkC0OFMh|ryP NVv3PI5wW0GQR1XS
NOS-1 NYnuOVZtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXkTWM2OD14Nz62PFY4KM7:TR?= MWTTRW5ITVJ?
RH-1 NUnTXWtHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjGWZNwUUN3ME22PE42PTV7IN88US=> M2nWZXNCVkeHUh?=
HuH-7 NEDueVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTZ7LkGwNFIh|ryP MkfXV2FPT0WU
NUGC-3 Mmf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTdyLkm2OFQh|ryP Ml7uV2FPT0WU
IST-SL1 NWLTVYV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jnO2lEPTB;N{GuNlMzPCEQvF2= NH7ZcY1USU6JRWK=
SHP-77 NEO0U5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nNSWlEPTB;N{KuNVE6KM7:TR?= NVz3OJFxW0GQR1XS
SW1116 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PNe2lEPTB;N{KuNVQxPyEQvF2= M4fy[XNCVkeHUh?=
LK-2 MnnUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTd{Lki2NVIh|ryP Mn3LV2FPT0WU
U-2-OS MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rwb2lEPTB;N{OuNVgzQCEQvF2= NF7nVWhUSU6JRWK=
KYSE-150 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTd|Lk[xNVgh|ryP NFfJZ|hUSU6JRWK=
U251 Mlu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;5W4NuUUN3ME23N{45OTZ6IN88US=> NUjsXGhqW0GQR1XS
HT-1080 NYrMTW5HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXy1S|FpUUN3ME23OE4zPTd2IN88US=> MnzxV2FPT0WU
NCI-H1437 NFTjTo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvrTWM2OD15ND62N|I3KM7:TR?= M3HMS3NCVkeHUh?=
HPAF-II MmjwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYCxXWxFUUN3ME23OE43PDF3IN88US=> M17YV3NCVkeHUh?=
Becker M4nxZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1f2WmlEPTB;N{WuNVExPSEQvF2= M1KzNXNCVkeHUh?=
PANC-10-05 M4K1T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3OxTGlEPTB;N{WuNlg3PSEQvF2= MXzTRW5ITVJ?
TYK-nu M2H5[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTd3LkOxNFIh|ryP MnzRV2FPT0WU
SKG-IIIa MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;KTWM2OD15NT6zN|QyKM7:TR?= M1jydHNCVkeHUh?=
NCI-H1993 NVGwUY82T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjpTWM2OD15NT63N|k4KM7:TR?= MWjTRW5ITVJ?
S-117 NGG2[ldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTd3Lke1OUDPxE1? MXPTRW5ITVJ?
HuO9 NUX4[phvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrNTWM2OD15Nj6wNFQ5KM7:TR?= M3;DVXNCVkeHUh?=
CAL-51 NETJOpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjKdY9CUUN3ME23Ok4yODB4IN88US=> NFrEVZFUSU6JRWK=
BT-20 MnX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTd4LkKxOlgh|ryP M2TWSHNCVkeHUh?=
LB831-BLC MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPRfZB7UUN3ME23Ok4{OjF5IN88US=> M3GwUXNCVkeHUh?=
LB1047-RCC NFHHOoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPxXoJ1UUN3ME23Ok44PjF7IN88US=> NGPSRmpUSU6JRWK=
ES8 Mn7XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LyeGlEPTB;N{iuNFA4OyEQvF2= M2fBeHNCVkeHUh?=
UMC-11 MkHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmnoTWM2OD15OD6wPFg1KM7:TR?= M{TTdXNCVkeHUh?=
NCI-H226 NU\3NJFNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2rtNWlEPTB;N{iuNVMyPSEQvF2= MnPUV2FPT0WU
IGROV-1 NVSzdVFVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;le2NbUUN3ME23PE42PTVizszN MmiwV2FPT0WU
BHY M1TSVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{i5bWlEPTB;N{muOVk3KM7:TR?= MULTRW5ITVJ?
MKN45 NI\5SYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PONWlEPTB;OECuN|E4PCEQvF2= MV7TRW5ITVJ?
IA-LM NV:yRmdQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fwRWlEPTB;OECuN|UyOSEQvF2= MnjLV2FPT0WU
WM-115 NFfSXG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7GbpJKSzVyPUixMlQyOTRizszN MU\TRW5ITVJ?
VMRC-RCZ MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmGwTWM2OD16MT60OFI2KM7:TR?= NYHxUVM2W0GQR1XS
NCI-H2291 M3\E[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXrTWM2OD16MT62PFQzKM7:TR?= MUjTRW5ITVJ?
MCF7 MkPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;T[5Z3UUN3ME24N{4zQDl5IN88US=> NHnwNYNUSU6JRWK=
639-V Mom1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzrTWM2OD16ND62NVI6KM7:TR?= NITpeYRUSU6JRWK=
RT4 NWH0WYwxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmn3TWM2OD16NT6yOFA{KM7:TR?= NWH1bGVNW0GQR1XS
NCI-H2405 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TrSWlEPTB;OEWuNlk6PyEQvF2= MWPTRW5ITVJ?
CAL-33 NXX0ZWJPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzP[3FKSzVyPUi1MlY{OTVizszN NWTYTVdNW0GQR1XS
BT-549 NFfzUpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTh3LkmzPFIh|ryP M1vQbHNCVkeHUh?=
HT-1197 M{nLPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HST2lEPTB;OEiuNVA4PyEQvF2= NWrlToFLW0GQR1XS
G-361 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYD4Oo1HUUN3ME24PE4yPDd2IN88US=> NHL1NJJUSU6JRWK=
LS-411N MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEexdm9KSzVyPUi5MlM{QDhizszN NWHSPGNjW0GQR1XS
HT-144 NFfFW2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\ofJZKSzVyPUi5MlU6QTJizszN MmiwV2FPT0WU
DJM-1 Mo\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoGxTWM2OD16OT64PFQ2KM7:TR?= MWXTRW5ITVJ?
CAL-12T MorsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTlyLke4PVUh|ryP MmDTV2FPT0WU
MKN7 NFj4SZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTlzLkO1OVEh|ryP M3HkRXNCVkeHUh?=
HuP-T3 NIjuWFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4K2bWlEPTB;OU[uNVAxPCEQvF2= NEXW[45USU6JRWK=
SK-MG-1 M4TXcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTl4LkGzOFgh|ryP MlXPV2FPT0WU
NCI-H2347 NI\MbnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvQc4prUUN3ME25PE4yODR{IN88US=> NX3sZYl4W0GQR1XS
SW872 NInZVIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTFyMD60OVYh|ryP NIfLPJBUSU6JRWK=
COLO-829 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fDS2lEPTB;MUCwMlU2OyEQvF2= NX24SWc5W0GQR1XS
MC-IXC NGLtSYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTLZ21mUUN3ME2xNFAvPjF3IN88US=> NEG4R45USU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pS6 / S6 / pS6K / S6K1 / pAKT / AKT ; 

PubMed: 28626016     


Western blot of cell lysates from neurons treated with PF-4708671, rapamycin, and Torin-2, individually or in combination for the indicated times.

p-mTOR / mTOR / p-p70S6K / p70S6K ; 

PubMed: 30423811     


As determined using Western blotting, the phospho-p70S6K (p-p70S6K) inhibitor (PF-4708671) reduced the expression of phospho-mTOR (p-mTOR) and p-p70S6K in SCC-4 and SCC-25 cells. SCC-4 and SCC-25 cells were not treated as negative control (NC) or treated with 20 µM PF-4708671 for 6 h (6 h), 12 h (12 h), 18 h (18 h), 1 day (1 d), 2 days (2 d), and 3 days (3 d). Western blot analysis was performed to examine the expression levels of p-mTOR, mTOR, p-p70S6K, p70S6K, and β-actin.

28626016 30423811
Growth inhibition assay
Cell viability; 

PubMed: 30423811     


Inhibitory effects of various doses (0.5, 1, 2, 5, 10, 20, and 40 µM) of PF-4708671 on SCC-4 (B) and SCC-25 (C) cells were assessed using MTT assay after 72 h of treatment. Data presented are the mean and standard error of the mean of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate the statistical significance of differences between cells with and without treatment. t (red ** and *** for SCC-4 and blue *, ** and *** for SCC-25).

30423811

Protocol

Solubility (25°C)

In vitro DMSO 30 mg/mL (76.84 mM)
Ethanol 8 mg/mL (20.49 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 390.41
Formula

C19H21F3N6

CAS No. 1255517-76-0
Storage powder
in solvent
Synonyms N/A
Smiles CCC1=CN=CN=C1N2CCN(CC2)CC3=NC4=C(N3)C=C(C=C4)C(F)(F)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    Whether the in vivo formula "30% PEG400+0.5% Tween80+5% Propylene glycol" for S2163 is suitable for i.p. or not?

  • Answer:

    The formula "30% PEG400+0.5% Tween80+5% Propylene glycol" on our website is an oral one. It is a suspension.

S6 Kinase Signaling Pathway Map

Related S6 Kinase Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID