Nelfinavir (AG 1343) Mesylate

For research use only.

Catalog No.S4282 Synonyms: Viracept

8 publications

Nelfinavir (AG 1343) Mesylate Chemical Structure

CAS No. 159989-65-8

Nelfinavir Mesylate (Viracept, AG1343) is a potent HIV protease inhibitor with Ki of 2 nM.

Size Price Stock Quantity  
USD 137 In stock
USD 687 In stock
USD 2747 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Nelfinavir (AG 1343) Mesylate has been cited by 8 publications

Purity & Quality Control

Choose Selective HIV Protease Inhibitors

Biological Activity

Description Nelfinavir Mesylate (Viracept, AG1343) is a potent HIV protease inhibitor with Ki of 2 nM.
Targets
HIV protease [1]
(Cell-free assay)
2 nM(Ki)
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa M1vBUWZ2dmO2aX;uJIF{e2G7 MYTUVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBVTUFidYD0ZYtmKCiWRVG6JFExKHWPKTDpckBQS1RzLXX4dJJme3OrbnegTIVN[SClZXzsd{whUUN3ME2yNU45|ryPLh?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZ6MUO3PEc,OTB4OEGzO|g9N2F-
Caco-2 NID6VmRHfW6ldHnvckBie3OjeR?= MmXEWHBgXFKDTmPQU3JVTVJ8IHnubIljcXSrb36gc4YhTGG3bn;yeYJq[2mwIITyZY5{\XCrdHjlcIlidCC2cnHud5BwenRiKHLhd4FtKHSxIHHwbYNidCliKFTheY5wenWkaXPpclohPSC3TTmgbY4hS2Glbz2yJINmdGy|LDDJR|UxRTFwNN88UU4> M2P1cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOEKwNVM4Lz5zMEiyNFE{PzxxYU6=
MT-4 NEfW[2lCdnSrdnnyZYwh[XO|YYm= NGHSZYVCdnSrLVjJWk0yKGGldHn2bZR6KGGpYXnud5QhX2muZDD0fZBmKH[rcoXzJIlvKE2WLUSgZ4VtdHNuIFXEOVA:OC5yMt88UU4> NGS4Vog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW0N|Y4Pyd-MUG1OFM3Pzd:L3G+
MT-4 Mk\pRY51cX[rcnHsJIF{e2G7 M{X5cWFvfGlvSFnWMVEh[WO2aY\peJkh[WejaX7zeEBufXSjboSgTGlXNTFiaX6gUXQuPCClZXzsd{ApdXW2YYTpc44he2WuZXP0[YQhf2m2aDDjc41xd3WwZDCxLUwhTUR3ME2wMlA4|ryPLh?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTV2M{[3O{c,OTF3NEO2O|c9N2F-
MT-4 M4nZ[WFvfGm4aYLhcEBie3OjeR?= NVjheFdjSW62aT3ITXYuOSCjY4Tpeol1gSCjZ3HpcpN1KG23dHHueEBJUVZvMTDpckBOXC12IHPlcIx{KCivdYTheIlwdiC|ZXzlZ5Rm\CC5aYToJJJqfG:wYY\pdkktKEWGNUC9NE4yOc7:TT6= NEDsRVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW0N|Y4Pyd-MUG1OFM3Pzd:L3G+
MT-4 NWDlcXE6SW62aY\pdoFtKGG|c3H5 MUnBcpRqNUiLVj2xJIFkfGm4aYT5JIFo[Wmwc4SgcZV1[W62IFjJWk0yKGmwIF3UMVQh[2WubIOgLI12fGG2aX;uJJNmdGWldHXkJJdqfGhic3HxeYlv[X[rcjmsJGVFPTB;MD6xNu69VS5? M2DPdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNUSzOlc4Lz5zMUW0N|Y4PzxxYU6=
MT-4 NXHFcHdDSW62aY\pdoFtKGG|c3H5 M{OzXmFvfGlvSFnWMVEh[WO2aY\peJkh[WejaX7zeEBufXSjboSgTGlXNTFiaX6gUXQuPCClZXzsd{ApdXW2YYTpc44he2WuZXP0[YQhf2m2aDDu[YxncW6jdnnyLUwhTUR3ME2yMlQ1|ryPLh?= NWrJc2ZVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1OFM3PzdpPkGxOVQ{Pjd5PD;hQi=>
LLC-PK1 M1zCeGZ2dmO2aX;uJIF{e2G7 MnvhTY5pcWKrdHnvckBw\iCSLXfsfYNweHKxdHXpckwhdW:3c3WgUE1u\HJzYTDlfJBz\XO|ZXSgbY4hVEyFLWDLNUBmeGm2aHXsbYFtKGOnbHzzJJV{cW6pIHPhcINmcW5vQV2gdI9t[XKrc3H0bY9vKGG|c3H5MEBKSzVyPUKuN:69VS5? NFrGV|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[5PVM5QSd-MUK2PVk{QDl:L3G+
LLC-PK1 MYfGeY5kfGmxbjDhd5NigQ>? NX34dmc2XFChVGLBUnNRV1KWRWK6JIlvcGmkaYTpc44hd2ZiQ3HsZ4Vqdi2DTTDl[oZtfXhiaX6gUYRzOWFvZYjwdoV{e2mwZzDMUGMuWEtzIHPlcIx{NCCLQ{WwQVIvO87:TT6= NYLJOZoyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2PVk{QDlpPkGyOlk6Ozh7PD;hQi=>
LLC-PK1 MVzGeY5kfGmxbjDhd5NigQ>? NGPnOYJKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvNCCqdX3hckBNNU2GUkGg[ZhxemW|c3XkJIlvKEyOQz3QT|Eh\XCrdHjlcIlidCClZXzsd{B2e2mwZzDjZYxk\WmwLVHNJJBwdGG{aYPheIlwdiCjc4PhfUwhUUN3ME2zMlTPxE1w NFPpdlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[5PVM5QSd-MUK2PVk{QDl:L3G+
LLC-PK1 MYHGeY5kfGmxbjDhd5NigQ>? NVHnZYpQXFChVGLBUnNRV1KWRWK6JIlvcGmkaYTpc44hd2ZiQ3HsZ4Vqdi2DTTDl[oZtfXhiaX6gUWRTOS2neIDy[ZN{cW6pIFzMR{1RUzFiY3XscJMtKEmFNUC9N{41|ryPLh?= NWL1dVlqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2PVk{QDlpPkGyOlk6Ozh7PD;hQi=>
HEK NVXYRmhwTnWwY4Tpc44h[XO|YYm= MnLuWHBgXFKDTmPQU3JVTVJ8IHnuZ5Jm[XOnIHnuJI1qfG:6YX70do9v\SCrboTyZYNmdGy3bHHyJIFk[3WvdXzheIlwdiCrbjDCR3JRNWW6cILld5NqdmdiSFXLJINmdGy|LDDJR|UxRTF{LkZOwG0v MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTByN{GwNkc,OTVyMEexNFI9N2F-
CEM-SS NYrXdI57SW62aY\pdoFtKGG|c3H5 M1nWNlYh\GG7cx?= NX7UNJVGSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTCzRkBqdm[nY4Tl[EBqdiCqdX3hckBETU1vU2OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYTobYMh\W[oZXP0JIFnfGW{IE[g[IF6eyCkeTDNWHMh[XO|YYmsJGVFPTB;MD6wN:69VS5? M{j5SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MkG3NlgyLz5zNUKxO|I5OTxxYU6=
HOG.R5 MW\BcpRqfmm{YXygZZN{[Xl? MVe0JIRigXN? MXzBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKDOEIHnu[oVkfGWmIHnuJIh2dWGwIFjPS{5TPSClZXzsd{Bi\nSncjC0JIRigXNuIFnDOVA:OC5yOd88UU4> MoTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV{MUeyPFEoRjF3MkG3NlgyRC:jPh?=
MT-4 NWHpN3ljTnWwY4Tpc44h[XO|YYm= NGHrZZlG\m[nY4TpeoUh\G:|ZTDv[kBkd22yb4Xu[EBz\XG3aYLl[EB1dyCrbnjpZol1KHKncHzpZ4F1cW:wIH;mJIh2dWGwIHntcZVvd2SnZnnjbYVv[3lidnnyeZMhfHmyZTCxJIlvKE2WLUSgZ4VtdHNuIFXEOVA:OC5yMt88UU4> NYPCV4RKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1N|c{PTBpPkG1OVM4OzVyPD;hQi=>
MT4 MlnDRY51cX[rcnHsJIF{e2G7 M4\IcWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiSGjCNkBqdiCPVESgZ4VtdHNuIFnDOVA:OC5|Mt88UU4> M3:4[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEW4OVA2Lz5zNkS1PFUxPTxxYU6=
MT4 NXfSWpcxSW62aY\pdoFtKGG|c3H5 MYPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKEWSMUOgbY4hVVR2IHPlcIx{NCCLQ{WwQVAvPDYQvF2u NVL3ZmI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0OVg2ODVpPkG2OFU5PTB3PD;hQi=>
PHA-PBMC MWrBcpRqfmm{YXygZZN{[Xl? MlPsRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCEQT3MJIl{d2yjdHWgbY4hcHWvYX6gVGhCNVCETVOgZ4VtdHNuIFnDOVA:OC5yMEhOwG0v MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjlzM{exOEc,OTZ7MUO3NVQ9N2F-
MT2 M1jZfGFvfGm4aYLhcEBie3OjeR?= NETVTIJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIFzBTUBqe2:uYYTlJIlvKGi3bXHuJG1VOiClZXzsd{whUUN3ME2wMlAy|ryPLh?= MkDXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MUO3NVQoRjF4OUGzO|E1RC:jPh?=
PHA-PBMC M2XZbmFvfGm4aYLhcEBie3OjeR?= M{LqXGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTFHJJIl{d2yjdHWgbY4hcHWvYX6gVGhCNVCETVOgZ4VtdHNuIFnDOVA:OC5yMUVOwG0v MnjUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MUO3NVQoRjF4OUGzO|E1RC:jPh?=
MT2 NWfkb21ySW62aY\pdoFtKGG|c3H5 Mm\3RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOiCHSF:gbZNwdGG2ZTDpckBpfW2jbjDNWFIh[2WubIOsJGlEPTB;MD6wNu69VS5? MmDJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MUO3NVQoRjF4OUGzO|E1RC:jPh?=
PHA-PBMC MkTQRY51cX[rcnHsJIF{e2G7 Mlr2RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgcZVtfGliZIL1[{1z\XOrc4ThcpQhUEmYMTDFWEB3[XKrYX70JIlvKGi3bXHuJHBJSS2SQl3DJINmdGy|LDDJR|UxRTBwMEOy{txONg>? MmnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MUO3NVQoRjF4OUGzO|E1RC:jPh?=
PHA-PBMC NXPQR|k6SW62aY\pdoFtKGG|c3H5 NX23bYRPSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhdXWudHmg[JJ2\y2{ZYPpd5RidnRiSFnWNUBIKH[jcnnhcpQhcW5iaIXtZY4hWEiDLWDCUWMh[2WubIOsJGlEPTB;MD6xO:69VS5? NHX3dm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmxN|cyPCd-MU[5NVM4OTR:L3G+
PHA-PBMC NHPTNZdCdnSrdnnyZYwh[XO|YYm= MlzORY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgcZVtfGliZIL1[{1z\XOrc4ThcpQhUEmYMTDDJJZiemmjboSgbY4hcHWvYX6gVGhCNVCETVOgZ4VtdHNuIFnDOVA:OC5|Md88UU4> M2X6[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUGzO|E1Lz5zNkmxN|cyPDxxYU6=
MT2 MWHBcpRqfmm{YXygZZN{[Xl? NHjD[lRCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZ{IFXIU{BqdiCPVEKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyMkSgS4FoKHC{b4TlbY4h\XiycnXzd4lwdiCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wN:69VS5? NVG5XJFRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT2 NEnMdZNCdnSrdnnyZYwh[XO|YYm= M4\4UmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTFHJJIlvKE2WMjDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHB{NDDHZYcheHKxdHXpckBmgHC{ZYPzbY9vKGK7IF3UWEBie3OjeTygSWM2OD1yLkCzNu69VS5? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN5MUixNUc,OTd|N{G4NVE9N2F-
MT4 NGXlelRCdnSrdnnyZYwh[XO|YYm= NVTRT5RvSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDOUFQuOyCrbjDNWFQh[2WubIOgZpkhVVSWIHHzd4F6NCCHQ{WwQVAvODN|zszNMi=> MmX4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd|N{G4NVEoRjF5M{exPFEyRC:jPh?=
MT4 MYrBcpRqfmm{YXygZZN{[Xl? MXrBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKEeUTEm4NFY2eDJyIHnuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJAzPCCJYXegdJJwfGWrbjDlfJBz\XO|aX;uJIJ6KE2WVDDhd5NigSxiRVO1NF0xNjB6zszNMi=> M2\V[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{exPFEyLz5zN{O3NVgyOTxxYU6=
MT4 NHjr[G9CdnSrdnnyZYwh[XO|YYm= M{n2R2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJJqfG:wYY\pdk1z\XOrc4ThcpQhUEmYMTDpckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxOjRiR3HnJJBzd3SnaX6g[ZhxemW|c3nvckBjgSCPVGSgZZN{[XluIFXDOVA:OC5{Md88UU4> M1rP[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{exPFEyLz5zN{O3NVgyOTxxYU6=
MT4 MWrBcpRqfmm{YXygZZN{[Xl? M{naS2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIFueHKnbnH2bZIuemW|aYP0ZY51KEiLVkGgbY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheDJ2IFfh[{Bxem:2ZXnuJIV5eHKnc4Ppc44h[nliTWTUJIF{e2G7LDDFR|UxRTBwMkNOwG0v MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN5MUixNUc,OTd|N{G4NVE9N2F-
MT2 MYHBcpRqfmm{YXygZZN{[Xl? M2PndGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjJiUl;EJIlvKE2WMjDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHB{NDDHZYcheHKxdHXpckBmgHC{ZYPzbY9vKGK7IF3UWEBie3OjeTygSWM2OD1yLkK0{txONg>? NIXKcHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{O3NVgyOSd-MUezO|E5OTF:L3G+
MT4 NH7reZVCdnSrdnnyZYwh[XO|YYm= NHfadldCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBifGG8YX7heolzNXKnc3nzeIFvfCCKSW[xJIlvKE2WNDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHB{NDDHZYcheHKxdHXpckBmgHC{ZYPzbY9vKGK7IF3UWEBie3OjeTygSWM2OD1yLkK3{txONg>? M4fx[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{exPFEyLz5zN{O3NVgyOTxxYU6=
MT4 M4PqdWFvfGm4aYLhcEBie3OjeR?= NUC1PYg3SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDHVmw6QDB4NYCzNEBqdiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyMkSgS4FoKHC{b4TlbY4h\XiycnXzd4lwdiCkeTDNWHQh[XO|YYmsJGVEPTB;MD6yO:69VS5? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN5MUixNUc,OTd|N{G4NVE9N2F-
MT4 MXvBcpRqfmm{YXygZZN{[Xl? MVvBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKEeUTEm4NFY2eDRyIHnuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJAzPCCJYXegdJJwfGWrbjDlfJBz\XO|aX;uJIJ6KE2WVDDhd5NigSxiRVO1NF0xNjN2zszNMi=> NUjWR3VvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT4 MV7BcpRqfmm{YXygZZN{[Xl? NXLwNm06SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhcWSrbnH2bZIuemW|aYP0ZY51KEiLVkGgbY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheDJ2IFfh[{Bxem:2ZXnuJIV5eHKnc4Ppc44h[nliTWTUJIF{e2G7LDDFR|UxRTBwNEhOwG0v MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN5MUixNUc,OTd|N{G4NVE9N2F-
MT4 MVvBcpRqfmm{YXygZZN{[Xl? MkPHRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sgd4FyfWmwYY\pdk1z\XOrc4ThcpQhUEmYMTDpckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxOjRiR3HnJJBzd3SnaX6g[ZhxemW|c3nvckBjgSCPVGSgZZN{[XluIFXDOVA:OC52ON88UU4> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN5MUixNUc,OTd|N{G4NVE9N2F-
MT4 MXfBcpRqfmm{YXygZZN{[Xl? MWHBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDsc5BqdmG4aYKtdoV{cXO2YX70JGhKXjFiaX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdFI1KEejZzDwdo91\WmwIHX4dJJme3Orb36gZpkhVVSWIHHzd4F6NCCHQ{WwQVAvPDoQvF2u NUHFPINURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT2 M4K3cGN6fG:2b4jpZ4l1gSCjc4PhfS=> NWq1b2g5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVVR{IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheDJ2IFfh[{Bxem:2ZXnuJIV5eHKnc4Ppc44h[nliTWTUJIF{e2G7LDDDR|UxRThwMd88UU4> MlPMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd|N{G4NVEoRjF5M{exPFEyRC:jPh?=
MT4 MXTBcpRqfmm{YXygZZN{[Xl? MkHYRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSB|QjDpckBOXDRiY3XscJMh[nliTWTUJIF{e2G7LDDFR|UxRTBwMEG2{txONg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV|N{[yPEc,OTd3M{e2Nlg9N2F-
MT4 MUHDfZRwfG:6aXPpeJkh[XO|YYm= NXexTYY5S3m2b4TvfIlkcXS7IHHnZYlve3RiTWS0JINmdGy|IHL5JG1VXCCjc4PhfUwhVEN3ME2xNe69VS5? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV|N{[yPEc,OTd3M{e2Nlg9N2F-
MT4 NWfnWJRWSW62aY\pdoFtKGG|c3H5 M4C1SFUh\GG7cx?= MoHzRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQTEStN{Bqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYX\0[ZIhPSCmYYnzJIJ6KE2WVDDhd5NigSxiRVO1NF0xNjBzON88UU4> NIrlNo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W3Olg1QCd-MUe1O|Y5PDh:L3G+
MT4 MUjBcpRqfmm{YXygZZN{[Xl? NGS5[3U2KGSjeYO= NFTjNnpCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZ{IF3TJIlv\mWldHXkJIlvKGi3bXHuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolzfXNvaX7keYNm\CCleYTvdIF1cGmlIHXm[oVkfCCjZoTldkA2KGSjeYOgZpkhVVSWIHHzd4F6NCCHQ{WwQVAvODR6zszNMi=> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV5Nki0PEc,OTd3N{[4OFg9N2F-
CEM M4ruTmFvfGm4aYLhcEBie3OjeR?= NILsNHM4KGSjeYO= MX7BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYzKFKRRDD3bZRpKHC{b4TlZZNmKEdzN16vWlQ4SSCvdYTheIlwdiCrbn\lZ5Rm\CCrbjDoeY1idiCFRV2gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{Bxem:mdXP0bY9vKGGodHXyJFch\GG7czDifUBN\W62aT3SWEBi[3Srdnn0fUBie3OjeTygSWM2OD1yLkJOwG0v NUniTmdFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1O|Y5PDhpPkG3OVc3QDR6PD;hQi=>
CEM MkD4RY51cX[rcnHsJIF{e2G7 MXu3JIRigXN? NYW2V2dnSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMjDSU2Qhf2m2aDDwdo91\WG|ZTDWOFdCKG23dHH0bY9vKGmwZnXjeIVlKGmwIHj1cYFvKEOHTTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzJJBzd2S3Y4Tpc44h[W[2ZYKgO{Bl[Xm|IHL5JGxmdnSrLWLUJIFkfGm4aYT5JIF{e2G7LDDFR|UxRTBwMUe3{txONg>? NVzpXWZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1O|Y5PDhpPkG3OVc3QDR6PD;hQi=>
CEM MlrGRY51cX[rcnHsJIF{e2G7 MX63JIRigXN? NVfGTFZISW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMjDSU2Qhf2m2aDDwdo91\WG|ZTDHNVdPKG23dHH0bY9vKGmwZnXjeIVlKGmwIHj1cYFvKEOHTTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzJJBzd2S3Y4Tpc44h[W[2ZYKgO{Bl[Xm|IHL5JGxmdnSrLWLUJIFkfGm4aYT5JIF{e2G7LDDFR|UxRTBwMUm5{txONg>? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV5Nki0PEc,OTd3N{[4OFg9N2F-
CEM Mn\FRY51cX[rcnHsJIF{e2G7 MXG3JIRigXN? NVW4XIZGSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5Qhf2muZDD0fZBmKEiLVkKgVm9FKGmwZnXjeIVlKGmwIHj1cYFvKEOHTTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzJJBzd2S3Y4Tpc44h[W[2ZYKgO{Bl[Xm|IHL5JGxmdnSrLWLUJIFkfGm4aYT5JIF{e2G7LDDFR|UxRTBwMkix{txONg>? M2LhRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUe2PFQ5Lz5zN{W3Olg1QDxxYU6=
HEK293 NFfpUGFCdnSrdnnyZYwh[XO|YYm= Moe0OFghcHK| MmLpRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sge4lt\CC2eYDlJGhKXjFiTly0MVMhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDy[ZBtcWOjdHnvck1l\W[oZXP0bZZmKGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[U1j[XOnZDDwbIVvd3S7cHnjJIF{e2G7LDDFR|UxRTBwMEC1PO69VS5? NHLxdFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[zPFY6PCd-MUe2N|g3QTR:L3G+
MT4 MlT3RY51cX[rcnHsJIF{e2G7 NI\HfnQ3KGSjeYO= M1PNWWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRidInw[UBJUVZzIF7MOE0{KGmwZnXjeIVlKGmwIF3UOEBk\WyuczDh[pRmeiB4IHThfZMh[nliTWTUJIF{e2G7LDDFR|UxRTBwMEK5{txONg>? NXiycFRxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2N|g3QTRpPkG3OlM5Pjl2PD;hQi=>
MT4 M3ToZWN6fG:2b4jpZ4l1gSCjc4PhfS=> NUf4NHc2PiCmYYnz NHSxXlZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXDRiY3XscJMh[W[2ZYKgOkBl[Xm|IHL5JG1VXCCjc4PhfUwhS0N3ME24{txONg>? M2\qZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkO4Olk1Lz5zN{[zPFY6PDxxYU6=
HEK293 NVH3NlA4TnWwY4Tpc44h[XO|YYm= MUOwMlEzKHWP MonsTY5pcWKrdHnvckBw\iCKSW[xJG5NPC1|IIDyc5RmcW6|IHnuZ49zeG:{YYTpc44hcW62bzD2bZJqd26|IITyZY5{\mWldHXkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{B2dnC{b3Pld5Nm\CCpcEG2NEBGdnZicILveIVqdiCjdDCwMlEzKHWPIHL5JHdme3Sncn6gZoxwfA>? M2HtWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkS2OFExLz5zN{[0OlQyODxxYU6=
HEK293 MoPWSpVv[3Srb36gZZN{[Xl? MmjsNE4yOiC3TR?= NEDoZnVKdmirYnn0bY9vKG:oIFjJWlEhVkx2LUOgdJJwfGWrboOgbY5kd3Kyb4LheIlwdiCrboTvJJZqemmxboOgeJJidnOoZXP0[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KGGkZYLyZY51KEWwdjDwdo91\WmwIHH0JFAvOTJidV2gZpkhX2W|dHXyckBjdG:2 M1TaO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkS2OFExLz5zN{[0OlQyODxxYU6=
MDCK2 NIXpRVFKdmirYnn0bY9vKG:oIHj1cYFv NXvCfYxlUW6qaXLpeIlwdiCxZjDoeY1idiCPRGKxMYRmeGWwZHXueEBi[2O3bYXsZZRqd25ib3[gZ4Ft[2Wrbj3BUUBmgHC{ZYPz[YQhcW5iTVTDT|Ih[2WubIOsJGlEPTB;MUmuPe69VS5? NFKw[Wc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[2OFMzPyd-MUe2OlQ{Ojd:L3G+
MDR1 Mo\zSpVv[3Srb36gZZN{[Xl? MVOyNEB2VQ>? MYPE[YNz\WG|ZTDpckBRNWeueXPvdJJwfGWrbj3t[YRq[XSnZDD0[Y5w\m:4aYKg[Il{d3C{b4jpcEBnfW2jcnH0[UBm\m[udYigbY4hVUSFS{Kg[ZhxemW|c3nu[{BpfW2jbjDNSHIyKGOnbHzzJIF1KDJyIIXN NXHJTHBMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OlQ{OjdpPkG3OlY1OzJ5PD;hQi=>
CEM-SS M{H0dmFvfGm4aYLhcEBie3OjeR?= MYC1JIRigXN? MUXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKEyDSTDpcoZm[3SnZDDpckBpfW2jbjDDSW0uW1NiY3XscJMh[XO|ZYPz[YQh[XNicnX2[ZJ{\SC2cnHud4NzcXC2YYPlJIFkfGm4aYT5JIFnfGW{IEWg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6wNFLPxE1w NVLsdI8zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5OVA6PTVpPkG3PVUxQTV3PD;hQi=>
MOLT-4/3B M{PIbmFvfGm4aYLhcEBie3OjeR?= MnnYOEBl[Xm| NXy4Tld2SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTCzRkBqdm[nY4Tl[EBqdiCPT1zUMVQwO0JiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBu[XS3cnWgeolzfXNicnXs[YF{\SCvZXHzeZJm\CCjZoTldkA1KGSjeYOgc4YhcW6oZXP0bY9vKGK7IGLUJIF{e2G7LDDFR|UxRTBwMEK5{txONg>? NGT6fIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEOxOlUzOSd-MUizNVY2OjF:L3G+
MT4 NGDP[YlCdnSrdnnyZYwh[XO|YYm= Mnz4RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSB|QjDpckBpfW2jbjDNWFQh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidC2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1KGK7IF3UWEBu\XSqb3SsJGVEPTB;MD6wNVbPxE1w Mn3PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2Mk[xPVUoRjF6NEK2NVk2RC:jPh?=
MT4 MW\DfZRwfG:6aXPpeJkh[XO|YYm= MmXaR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXQ1KGOnbHzzJIJ6KE2WVDDhd5NigSxiQ1O1NF0yOc7:TT6= NWO2VnN4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0NlYyQTVpPkG4OFI3OTl3PD;hQi=>
MT4 NVmzOmFtSW62aYLleJJwfmm{YXygZZN{[Xl? NGfzTY1CdnSrcnX0do93cXKjbDDhZ5Rqfmm2eTDh[4FqdnO2IFj1cYFvKGmvbYXuc4Rm\mmlaXXuZ5khfmm{dYOgNUBPVDRwMzDpcoZm[3SnZDDpckBpfW2jbjDNWFQh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJ2ey2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1KGK7IF3UWEBie3OjeTygSWM2OD1yLkCyN:69VS5? MonsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NEG3NlYoRjF6NUSxO|I3RC:jPh?=
MT4 M3zxSGFvfGm{ZYTyc5ZqemGuIHHzd4F6 NX2ydmc1SW62aYLleJJwfmm{YXygZYN1cX[rdImgZYdicW6|dDDIeY1idiCrbX31co9l\W[rY3nlcoN6KH[rcoXzJFEhO0JiaX7m[YN1\WRiaX6gbJVu[W5iTWS0JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBjgSCPVGSgZZN{[XluIFXDOVA:OC5yMklOwG0v MoC3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NEG3NlYoRjF6NUSxO|I3RC:jPh?=
MT4 NUj1dmNLSW62aY\pdoFtKGG|c3H5 MlHIRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgVoV3\XK|ZTD0doFve2O{aYD0ZZNmKGmwaHnibZRwei2{ZYPpd5RidnRiSIXtZY4hcW2vdX7v[IVncWOrZX7jfUB3cXK3czCxJIlv\mWldHXkJIlvKGi3bXHuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYomgUXRVKGG|c3H5MEBGSzVyPUCuNFI6|ryPLh?= Ml7CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NEG3NlYoRjF6NUSxO|I3RC:jPh?=
MT4 NX\3W5VKSW62aY\pdoFtKGG|c3H5 NFzCO4tCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBvd25iboXjcIVwe2mmZTDy[ZZmenOnIITyZY5{[3KrcIThd4UhcW6qaXLpeI9zNXKnc3nzeIFvfCCKdX3hckBqdW23bn;k[YZq[2mnbnP5JJZqenW|IEGgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNvaX7keYNm\CCleYTvdIF1cGmlIHXm[oVkfCCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wN|XPxE1w MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV2MUeyOkc,OTh3NEG3NlY9N2F-
MT4 MkiwRY51cXKndILveolz[WxiYYPzZZk> M4PTZWFvfGm{ZYTyc5ZqemGuIHHjeIl3cXS7IHHnZYlve3RiSIXtZY4hcW2vdX7v[IVncWOrZX7jfUB3cXK3czCyJGVJVyCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYTobYMh\W[oZXP0JIJ6KE2WVDDhd5NigSxiRVO1NF0xNjB2Od88UU4> Mn3XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NEG3NlYoRjF6NUSxO|I3RC:jPh?=
MT4 NXji[VBpSW62aYLleJJwfmm{YXygZZN{[Xl? MmHmRY51cXKndILveolz[WxiYXP0bZZqfHliYXfhbY5{fCCKdX3hckBqdW23bn;k[YZq[2mnbnP5JJZqenW|IEKgVm9FKGmwZnXjeIVlKGmwIHj1cYFvKE2WNDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZpkhVVSWIHHzd4F6NCCHQ{WwQVAvODYQvF2u NF;K[Ik9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW0NVczPid-MUi1OFE4OjZ:L3G+
MT4 NHX0TlFCdnSrcnX0do93cXKjbDDhd5NigQ>? NUH2U|VGSW62aYLleJJwfmm{YXygZYN1cX[rdImgZYdicW6|dDDTbY1q[W5iaX3teY5w\GWoaXPp[Y5kgSC4aYL1d{BOSUNiMkWxJIlv\mWldHXkJIlvKGi3bXHuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYomgUXRVKGG|c3H5MEBGSzVyPUCuNFXPxE1w NY\Nd2hERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OFE4OjZpPkG4OVQyPzJ4PD;hQi=>
MT4 MmDTRY51cX[rcnHsJIF{e2G7 MWTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDQdo91\WG|ZTDpcohq[mm2b4KtdoV{cXO2YX70JGh2dWGwIHntcZVvd2SnZnnjbYVv[3lidnnyeZMhOSCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYTobYMh\W[oZXP0JIJ6KE2WVDDhd5NigSxiRVO1NF0xNjF5zszNMi=> NVnIVY5iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OFE4OjZpPkG4OVQyPzJ4PD;hQi=>
MT4 NVvXTVA1SW62aY\pdoFtKGG|c3H5 MYm1JJVO MneyRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQTEStN{Bp[XKkb4LpcochVDFyRj;MN|NHN012NlmvTVQ4Xi:TNUjFM3Y5OklxSUi0Wk9KQDWYIHHtbY5wKGGlaXSgd5Vje3SrdIX0bY9vKGmwIIDyc5Rm[XOnIHXuZ49lcW6pIILl[4lwdiCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyMkSgS4FoKHC{b4TlbY4heHKxZIXjeIlwdiC|ZXzlZ5Rm\CCjdDC1JJVOKG:oIFfSUE0xOjB|MTDifUBGNCCHQ{WwQVAvODFzzszNMi=> MnPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7NUW1NVgoRjF6OUW1OVE5RC:jPh?=
MT4 NX;HcFdoSW62aY\pdoFtKGG|c3H5 NF73RmRCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKE2WNDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHB{NDDnZYcheHKxdHXpckBxem:mdXP0bY9vKGK7IFXMTXNCNCCHQ{WwQVAvODF6zszNMi=> NEi0eIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEm1OVUyQCd-MUi5OVU2OTh:L3G+
MT2 MlXQRY51cX[rcnHsJIF{e2G7 MXK3JIRigXN? MmHCRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCOQVmgbY5n\WO2ZXSgbY4hcHWvYX6gUXQzKGOnbHzzJIFnfGW{IEeg[IF6eyCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wNlPPxE1w M3\LOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUW1OVE5Lz5zOEm1OVUyQDxxYU6=
MT4 NXyxUpJSSW62aY\pdoFtKGG|c3H5 MnrnOUB2VQ>? NXr3fZpVSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDOUFQuOyCqYYLic5JqdmdiTEGwSk9XOzKLL120OmkwUTV2TT:vRVcyXi:LOETWJIFucW6xIHHjbYQhe3Wkc4TpeJV1cW:wIHnuJJBzd3SnYYPlJIVv[2:maX7nJJJm\2mxbjDpcoZm[3SnZDDpckBpfW2jbjDNWFQh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDwNlQhT2GpIIDyc5RmcW5icILv[JVkfGmxbjDz[Yxm[3SnZDDheEA2KHWPIH;mJIFueHKnbnH2bZIh[nliRVzJV2EtKEWFNUC9NE4yP87:TT6= Mn\FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7NUW1NVgoRjF6OUW1OVE5RC:jPh?=
MT4 MmX0RY51cX[rcnHsJIF{e2G7 NV34OW1kOSC3TR?= MnjqRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQTEStN{Bp[XKkb4LpcochVDJ|ST;LOFNKN012NlmvTVUxVC:JNUHBM2E4OVZiYX3pco8h[WOrZDDzeYJ{fGm2dYTpc44hcW5icILveIVie2ViZX7jc4RqdmdicnXnbY9vKGmwZnXjeIVlKGmwIHj1cYFvKE2WNDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHB{NDDHZYcheHKxdHXpckBxem:mdXP0bY9vKHOnbHXjeIVlKGG2IEGgeW0hd2ZiYYThfoFv[X[rcjDifUBGVEmVQTygSWM2OD1yLkKx{txONg>? NEnxRlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEm1OVUyQCd-MUi5OVU2OTh:L3G+
MT4 NWXkPVl{SW62aY\pdoFtKGG|c3H5 MYG1JJVO MnPLRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQTEStN{Bp[XKkb4LpcochVTR4ST;WPFJHN0l6NG[gZY1qdm9iYXPp[EB{fWK|dHn0eZRqd25iaX6gdJJwfGWjc3Wg[Y5kd2SrbnegdoVocW:wIHnu[oVkfGWmIHnuJIh2dWGwIF3UOEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIICyOEBI[WdicILveIVqdiCycn;keYN1cW:wIIPlcIVkfGWmIHH0JFUhfU1ib3[gdol1d26jdnnyJIJ6KEWOSWPBMEBGSzVyPUCuNlTPxE1w MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl3NUWxPEc,OTh7NUW1NVg9N2F-
MT4 M{n5TGFvfGm4aYLhcEBie3OjeR?= MoraOUB2VQ>? MVjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKE6OND2zJIhiemKxcnnu[{BNOTCLL1e0PHYwUTV2Vj;MPVBOKGGvaX7vJIFkcWRic4Xid5RqfHW2aX;uJIlvKHC{b4TlZZNmKGWwY3;kbY5oKHKnZ3nvckBqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxOjRiR3HnJJBzd3SnaX6gdJJw\HWldHnvckB{\WynY4Tl[EBifCB3IIXNJI9nKHOjcYXpcoF3cXJiYomgSWxKW0FuIFXDOVA:OC5|Mt88UU4> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl3NUWxPEc,OTh7NUW1NVg9N2F-
MT4 NV;lTXhsSW62aY\pdoFtKGG|c3H5 Mme3NUB2VQ>? Mli1RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQTEStN{Bp[XKkb4LpcochVDFyRj;NOFZKN0l3NG[vWlgzSSCjbXnuc{Bi[2mmIIP1ZpN1cXS3dHnvckBqdiCycn;0[YF{\SCnbnPv[Ilv\yC{ZXfpc44hcW6oZXP0[YQhcW5iaIXtZY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheDJ2IFfh[{Bxem:2ZXnuJJBzd2S3Y4Tpc44he2WuZXP0[YQh[XRiMTD1UUBw\iCOb4DpcoF3cXJiYomgSWxKW0FuIFXDOVA:OC53Md88UU4> M364XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUW1OVE5Lz5zOEm1OVUyQDxxYU6=
MT4 NUDHXIk3SW62aY\pdoFtKGG|c3H5 MkTnOUB2VQ>? NIPON3hCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIF7MOE0{KGijcnLvdolv\yCOMUDGM2wzPElxTUS2TU9NPjOSL1G3NXYwTzd|Uz;WPFJVKGGvaX7vJIFkcWRic4Xid5RqfHW2aX;uJIlvKHC{b4TlZZNmKGWwY3;kbY5oKHKnZ3nvckBqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxOjRiR3HnJJBzd3SnaX6gdJJw\HWldHnvckB{\WynY4Tl[EBifCB3IIXNJI9nKGmwZHnuZZZqeiCkeTDFUGlUSSxiRVO1NF0xNjZ3zszNMi=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl3NUWxPEc,OTh7NUW1NVg9N2F-
HeLa M2e4cmFvfGm4aYLhcEBie3OjeR?= MoS2O|IhcHK| Mlj3RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCqYYLic5JqdmdiY3Hwd4llKEl{MEHWJI12fGGwdDDwdo91\WmwIHnu[oVkfGWmIHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5idnnyZYwhcW6oZXP0bY9vKGGodHXyJFczKGi{czDifUBj\XSjLXfhcIFkfG:|aXThd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjBzM988UU4> NIXZdGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUiwOVU4OSd-MUm4NFU2PzF:L3G+
HeLa M3z3e2FvfGm4aYLhcEBie3OjeR?= NE\KeXg4OiCqcoO= Mmf5RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sge4lt\CC2eYDlJGhKXjFiaX7m[YN1\WRiaX6gbJVu[W5iSHXMZUBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiC4aYLhcEBqdm[nY4Tpc44h[W[2ZYKgO|IhcHK|IHL5JIJmfGFvZ3HsZYN1d3OrZHHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODF4zszNMi=> MlLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6MEW1O|EoRjF7OEC1OVcyRC:jPh?=
HeLa M2P0dGFvfGm4aYLhcEBie3OjeR?= NFLWV2c4OiCqcoO= NG\hTm1CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIHjhdoJwemmwZzDzdIFk\XJicHXweIll\SCDMW[gcZV1[W62IIDyc5RmcW5iaX7m[YN1\WRiaX6gbJVu[W5iSHXMZUBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiC4aYLhcEBqdm[nY4Tpc44h[W[2ZYKgO|IhcHK|IHL5JIJmfGFvZ3HsZYN1d3OrZHHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODN6zszNMi=> Mn7rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6MEW1O|EoRjF7OEC1OVcyRC:jPh?=
cord blood mononuclear cells NXzpboFJSW62aY\pdoFtKGG|c3H5 NWXoOFNtPDhiaILz NIfuPYpCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEB4cWymIIT5dIUhUEmYMTDzeYJ1gXCnIFOgbY5n\WO2ZXSgbY4hcHWvYX6gZ49z\CCkbH;v[EBud26xboXjcIVieiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODF4N988UU4> MoPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MESxNlMoRjJyNEC0NVI{RC:jPh?=
cord blood mononuclear cells NUfneoUzSW62aY\pdoFtKGG|c3H5 MWC0PEBpenN? NEHWcpJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEB4cWymIIT5dIUhUEmYMTDzeYJ1gXCnIFPSSlAzZ0GJIHnu[oVkfGWmIHnuJIh2dWGwIHPvdoQh[myxb3SgcY9vd263Y3zlZZIh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB2ODDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUCuNFA2P87:TT6= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODRyNEGyN{c,OjB2MESxNlM9N2F-
cord blood mononuclear cells NX;2WXB5SW62aY\pdoFtKGG|c3H5 M2W1Z|Q5KGi{cx?= NXLqVWE6SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5Qhf2muZDD0fZBmKEiLVkGgd5VjfHmyZTDCJIlv\mWldHXkJIlvKGi3bXHuJINwemRiYnzvc4QhdW:wb371Z4xm[XJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODB7ON88UU4> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODRyNEGyN{c,OjB2MESxNlM9N2F-
cord blood mononuclear cells MXjBcpRqfmm{YXygZZN{[Xl? NWrU[4xiPDhiaILz MXnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKHO3YoT5dIUhS1KIMELfRWchcGG{Yn;ybY5oKHC{b4TlZZNmKHCxbIntc5JxcGm|bTDheEBKOzZicH;zbZRqd25iYX7kJG01PiCvdYThcpQh\2WwZTDpcoZm[3SnZDDpckBpfW2jbjDjc5JlKGKub3;kJI1wdm:wdXPs[YFzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiNEigbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlA2Pc7:TT6= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODRyNEGyN{c,OjB2MESxNlM9N2F-
cord blood mononuclear cells NGrjb2NCdnSrdnnyZYwh[XO|YYm= NHnTe5c1QCCqcoO= MoPBRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSC|dXL0fZBmKEOURkCyY2FIKGijcnLvdolv\yCycn;0[YF{\SCOM{PJMEBOPD[LLDDBO|FVKG23dHHueEBo\W6nIHHu[EBxd2y7bX;ydIhqe21iYYSgUVM3KHCxc3n0bY9vKGmwZnXjeIVlKGmwIHj1cYFvKGOxcnSgZoxwd2RibX;uc452[2ynYYKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBz\XCuaXPheIlwdiCjZoTldkA1QCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmNCCHQ{WwQVAvOjB{zszNMi=> NVTKV4hZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0NFQyOjNpPkKwOFA1OTJ|PD;hQi=>
cord blood mononuclear cells MX3BcpRqfmm{YXygZZN{[Xl? MlXTOFghcHK| Ml32RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSC|dXL0fZBmKENiaHHyZo9zcW6pIIDyc5Rm[XOnIFyyN2ktKE12NlmsJGw5QVRibYX0ZY51KGenbnWgZY5lKHCxbIntc5JxcGm|bTDheEBOOzZicH;zbZRqd25iaX7m[YN1\WRiaX6gbJVu[W5iY3;y[EBjdG:xZDDtc45wdnWlbHXhdkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHHmeIVzKDR6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhNCCHQ{WwQVAvOjd{zszNMi=> NWGxU2xyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0NFQyOjNpPkKwOFA1OTJ|PD;hQi=>
cord blood mononuclear cells NILOW3VCdnSrdnnyZYwh[XO|YYm= NGnQXHI1QCCqcoO= MUPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKHO3YoT5dIUhSiCqYYLic5JqdmdicILveIVie2ViREOwUkwhVTR4STygRVcyXCCjbnSgWlc4USCvdYThcpQh\2WwZTDhcoQheG:ueX3vdpBpcXOvIHH0JG0{PiCyb4PpeIlwdiCxZjDwdo91\WG|ZTDpcoZm[3SnZDDpckBpfW2jbjDjc5JlKGKub3;kJI1wdm:wdXPs[YFzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiNEigbJJ{KGK7IHz1Z4ktKEWFNUC9NU4yOzoQvF2u M2fHc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEC0NVI{Lz5{MESwOFEzOzxxYU6=
cord blood mononuclear cells Ml25RY51cX[rcnHsJIF{e2G7 MWW0PEBpenN? MUnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKHO3YoT5dIUhSiCqYYLic5JqdmdicILveIVie2ViREOwUkwhVDN|STygUVQ3USxiTki4SEBufXSjboSg[4Vv\SCjbnSgdI9tgW2xcoDobZNuKGG2IFmzOkBxd3OrdHnvckBqdm[nY4Tl[EBqdiCqdX3hckBkd3KmIHLsc49lKG2xbn;ueYNt\WG{IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXygdoVxdGmlYYTpc44h[W[2ZYKgOFghcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZItKEWFNUC9NU41PjQQvF2u MnfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MESxNlMoRjJyNEC0NVI{RC:jPh?=
cord blood mononuclear cells M{nOfGFvfGm4aYLhcEBie3OjeR?= MVu0PEBpenN? NV;ncGFYSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDzeYJ1gXCnIFOgbIFz[m:{aX7nJJBzd3SnYYPlJGQ{OE5uIFWzOWctKEl6NW[sJGw6OE1ibYX0ZY51KGenbnWgZY5lKHCxbIntc5JxcGm|bTDheEBKOzZicH;zbZRqd25iaX7m[YN1\WRiaX6gbJVu[W5iY3;y[EBjdG:xZDDtc45wdnWlbHXhdkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHHmeIVzKDR6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{LDDFR|UxRTNwNEez{txONg>? MmX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MESxNlMoRjJyNEC0NVI{RC:jPh?=
TZM-bl NIjMZZFCdnSrdnnyZYwh[XO|YYm= MkDIOFghcHK| NXi0VpBHSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDzeYJ1gXCnIFKgbIFz[m:{aX7nJJBzd3SnYYPlJJBwdHmvb4LwbIl{dSCjdDDNN|YheG:|aYTpc44hcW6oZXP0[YQhcW5iaIXtZY4hXFqPLXLsJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25iYX\0[ZIhPDhiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?= NX3EVXhmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0NFQyOjNpPkKwOFA1OTJ|PD;hQi=>
TZM-bl M{H0TWFvfGm4aYLhcEBie3OjeR?= NFrpOms1QCCqcoO= M{G5V2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFic4XieJlx\SCFUl[wNn9CTyCqYYLic5JqdmdicILveIVie2VicH;sfY1wenCqaYPtJIF1KEl|NjDwc5NqfGmxbjDpcoZm[3SnZDDpckBpfW2jbjDUXm0u[mxiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 MnvRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MESxNlMoRjJyNEC0NVI{RC:jPh?=
TZM-bl M2HDUmFvfGm4aYLhcEBie3OjeR?= NH:3dZI1QCCqcoO= M{P1ZmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFic4XieJlx\SCEIHjhdoJwemmwZzDwdo91\WG|ZTDwc4x6dW:{cHjpd40h[XRiSUO2JJBwe2m2aX;uJIlv\mWldHXkJIlvKGi3bXHuJHRbVS2kbDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFQ5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? MlPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MESxNlMoRjJyNEC0NVI{RC:jPh?=
MT4 NGDHW3BCdnSrdnnyZYwh[XO|YYm= M4HpW2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRidInw[UBJUVZzIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKE2WNDDj[YxteyCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wOFXPxE1w NY[2ZoQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0N|k3OTJpPkKwOFM6PjF{PD;hQi=>
MT4 MYPBcpRqfmm{YXygZZN{[Xl? NEGyZ5hCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIHX4dJJme3OrbnegdJJwfGWjc3WgUFExUS:OMkTJM201PklxVkiyTU9KQDSYIH31eIFvfCCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNic3Xs[YN1\WRiYX\0[ZIhPTBicHHzd4Fo\XNib3[gS3JNNTJzNjDifUBOXFRiYYPzZZktKEWFNUC9NE4yO87:TT6= Mlf3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2M{m2NVIoRjJyNEO5OlEzRC:jPh?=
MT4 M3vTWmFvfGm4aYLhcEBie3OjeR?= NUfOfZQzOSC3TR?= NEnzNXBCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIHX4dJJme3OrbnegdJJwfGWjc3WgUFExTi:PNE\NMGkwWTZzUTDteZRidnRiaX7m[YN1\WRiaX6gbJVu[W5iTWS0JINmdGy|IIPlcIVkfGWmIHH0JFEhfU1ib3[gS3JNNTN7NjDifUBOXFRiYYPzZZktKEWFNUC9NE4zOs7:TT6= NWjIR4RuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0N|k3OTJpPkKwOFM6PjF{PD;hQi=>
MT4 MoLBRY51cX[rcnHsJIF{e2G7 NYT5UpFiOSC3TR?= NETI[mlCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIHX4dJJme3OrbnegdJJwfGWjc3WgUFExTi:PNE\JM3Q6OVNibYX0ZY51KGmwZnXjeIVlKGmwIHj1cYFvKE2WNDDj[YxteyC|ZXzlZ5Rm\CCjdDCxJJVOKG:oIFfSUE0zPDZiYomgUXRVKGG|c3H5MEBGSzVyPUCuNlnPxE1w Mn3aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2M{m2NVIoRjJyNEO5OlEzRC:jPh?=
MT4 NHfYT2FCdnSrdnnyZYwh[XO|YYm= NF[3fYM2KHWP NXnLPZpjSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDlfJBz\XO|aX7nJJBzd3SnYYPlJGwyOEZxTUS2TU9KPTCYL1G3NXYwUTh2Vj;MPVBOKG23dHHueEBqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMhe2WuZXP0[YQh[XRiNTD1UUBw\iCjbYDy[Y5ifmm{IHL5JG1VXCCjc4PhfUwhTUN3ME2wMlPPxE1w MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR|OU[xNkc,OjB2M{m2NVI9N2F-
MT4 NWjXZ|dUSW62aY\pdoFtKGG|c3H5 M3;jOVUhfU1? MXXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKGW6cILld5NqdmdicILveIVie2ViTEGwSk9OPD[LL1m1OHYwXjh{QTDteZRidnRiaX7m[YN1\WRiaX6gbJVu[W5iTWS0JINmdGy|IIPlcIVkfGWmIHH0JFUhfU1ib3[gUI9xcW6jdnnyJIJ6KE2WVDDhd5NigSxiRVO1NF0xNjN{zszNMi=> M1;3dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO5OlEzLz5{MESzPVYyOjxxYU6=
MT4 MULBcpRqfmm{YXygZZN{[Xl? NI\TUJMyKHWP MYLBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKGW6cILld5NqdmdicILveIVie2ViTEGwSk9OPD[OL1m1NHYwSTdzVn31eIFvfCCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNic3Xs[YN1\WRiYYSgNUB2VSCxZjDHVmwuOjh4IHL5JG1VXCCjc4PhfUwhTUN3ME2wMlM4|ryPLh?= NIOybm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESzPVYyOid-MkC0N|k3OTJ:L3G+
MT4 MV\BcpRqfmm{YXygZZN{[Xl? MnzROUB2VQ>? MWjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKGW6cILld5NqdmdicILveIVie2ViTEGwTU9IPDiYL1m1OHYwSTdzVj;JPFRXN0x7MF2gcZV1[W62IHnu[oVkfGWmIHnuJIh2dWGwIF3UOEBk\WyuczDz[Yxm[3SnZDDheEA2KHWPIH;mJJNieXWrbnH2bZIh[nliTWTUJIF{e2G7LDDFR|UxRTBwNUJOwG0v NHjpTpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESzPVYyOid-MkC0N|k3OTJ:L3G+
BESM MWHBcpRqfHK7cHHuc5NwdWGuIHHzd4F6 M{j2ZVg5KGi{cx?= MXXBcpRqfHK7cHHuc5NwdWGuIHHjeIl3cXS7IHHnZYlve3RiVIL5dIFvd3OxbXGgZ5J2gmliYX3hd5Rq\2:2ZYOgbY5n\WO2ZXSgbY4hSkWVTTDj[YxteyCvZXHzeZJm\CCjZoTldkA5QCCqcoOgdI9{fGmwZnXjeIlwdiCkeTDIWHMh[XO|YYmsJGVEPTB;MUNOwG0v NV7xRmpYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1OFc5OTlpPkKwOVQ4QDF7PD;hQi=>
BESM MYTDfZRwfG:6aXPpeJkh[XO|YYm= Mo\3PFghcHK| MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBDTVOPIHPlcIx{KGGodHXyJFg5KGi{czDifUBJXFNiYYPzZZktKEWFNUC9N|LPxE1w MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV2N{ixPUc,OjB3NEe4NVk9N2F-
CMEC/D3 M2W5NmZ2dmO2aX;uJIF{e2G7 MVSzNEBucW6| M4[3WmlvcGmkaYTpc44hd2ZiUD3ndEBqdiCqdX3hckBEVUWFL1SzJINmdGy|IIXzbY5oKE6ERD3BZoEh[XNic4Xid5Rz[XSnIHHmeIVzKDNyIH3pcpMh[nliZnzve{BkgXSxbXX0dolkKGGwYXz5d4l{NCCLQ{WwQVEvP87:TT6= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTVyNUmyPEc,OzF3MEW5Nlg9N2F-
CMEC/D3 Mnr4SpVv[3Srb36gZZN{[Xl? Mn\UN|AhdWmwcx?= M1;zfWlvcGmkaYTpc44hd2ZiUD3ndEBqdiCqdX3hckBEVUWFL1SzJINmdGy|IIXzbY5oKGOjbHPlbY4uSU1iYYOgd5Vje3S{YYTlJIFnfGW{IEOwJI1qdnNiYomg[oxwfyCleYTvcYV1emmlIHHuZYx6e2m|LDDJR|UxRTMQvF2u MnrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3MEW5NlgoRjNzNUC1PVI5RC:jPh?=
12D7-MDR MWDGeY5kfGmxbjDhd5NigQ>? NE[4OZY{OCCvaX7z MXTJcohq[mm2aX;uJI9nKFBvZ4CgbY4hcHWvYX6gNVJFPy2PRGKgZ4VtdHNidYPpcochVkKGLVHiZUBieyC|dXLzeJJifGViYX\0[ZIhOzBibXnud{BjgSCobH;3JIN6fG:vZYTybYMh[W6jbInzbZMtKEmFNUC9OU45|ryPLh?= NUTUTYZCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1NFU6OjhpPkOxOVA2QTJ6PD;hQi=>
12D7-MDR MoXaSpVv[3Srb36gZZN{[Xl? M1TPc|MxKG2rboO= Mk\DTY5pcWKrdHnvckBw\iCSLXfwJIlvKGi3bXHuJFEzTDdvTVTSJINmdGy|IIXzbY5oKGOjbHPlbY4uSU1iYYOgd5Vje3S{YYTlJIFnfGW{IEOwJI1qdnNiYomg[oxwfyCleYTvcYV1emmlIHHuZYx6e2m|LDDJR|UxRTlwMd88UU4> Mo\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3MEW5NlgoRjNzNUC1PVI5RC:jPh?=
12D7 Ml\zRY51cX[rcnHsJIF{e2G7 NHnsSVg{KGSjeYO= M1PqTmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTFHJJIlv\mWldHXkJIlvKGi3bXHuJFEzTDdiY3XscJMhd3[ncnX4dJJme3OrbnegdE1oeCCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hWDJ2IHzleoVtKGG2IEG6NUBz[XSrbzDv[kB1\XO2IHPvcZBwfW6mIITvJIFj[WOjdnnyJIlv[3WkYYTl[EBnd3JiMzDkZZl{KG[xbHzve4VlKGK7IH\vcIxwf2WmIHL5JJJmeGyjY3Xt[Y51KG:oIH\y[ZNpKG2nZHn1cUBkd262YXnubY5oKGOxbYDveY5l MoXiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3MEW5NlgoRjNzNUC1PVI5RC:jPh?=
12D7 MmDhRY51cX[rcnHsJIF{e2G7 NWjUO2tTOC5yODD0c{AyNjJ3IIXN NV33clVLOyCmYYnz M2DVXGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTFHJJIlv\mWldHXkJIlvKGi3bXHuJFEzTDdiY3XscJMhd3[ncnX4dJJme3OrbnegdE1oeCCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hWDJ2IHzleoVtKGG2IECuNFghfG9iMT6yOUB2VSCrbnP1ZoF1\WRiZn;yJFMh\GG7czDmc4xtd3enZDDifUBz\XCuYXPlcYVvfCCxZjDmdoV{cCCvZXTpeY0h[2:wdHHpcolv\yClb33wc5Vv\CCjbnSgcYVie3W{ZXSgZYZ1\XJiMzDkZZl{KGK7IFXMTXNC MmLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3MEW5NlgoRjNzNUC1PVI5RC:jPh?=
SJ-GBM2 Mo\hdWhVWyCjc4PhfS=> Mn3sdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= M{PSW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC MVzxTHRUKGG|c3H5 NWnwfo1MeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> NHnZNGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
U-2 OS NEXmZotyUFSVIHHzd4F6 MmLzdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFVvMjDPV{Bk\Wyucx?= NITIemU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Saos-2 MlzidWhVWyCjc4PhfS=> M{\X[ZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVYX;zMVIh[2WubIO= NFHs[HY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 M2e0NpFJXFNiYYPzZZk> MkC2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= M1zVbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD NEnvNlFyUFSVIHHzd4F6 NFnJWZByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUlSgZ4VtdHN? MlSzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R) M2jBTJFJXFNiYYPzZZk> NICzS|lyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTVegOlMhMDZvVFegVkkh[2WubIO= M4HsbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MT2 NVftepNtSW62aY\pdoFtKGG|c3H5 MorBOUBl[Xm| MlrQRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sge4lt\CC2eYDlJGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gUXQzKGOnbHzzJIF{e2W|c3XkJIF{KH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZYZ1\XJiNTDkZZl{KGK7IGjUWEBie3OjeTygSWM2OD1yLkCwOFnPxE1w M{P1b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU5PC9pPlPoSW1DVDxxYU6=
HepG2 NF;Q[I9EgXSxdH;4bYNqfHliYYPzZZk> MoSyOUBl[Xm| NH;lWm1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiNTDkZZl{KGK7IGjUWEBie3OjeTygR2M2OD15LkVOwG0v Ml;uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVg1Nyd-Q3jFUWJNRC:jPh?=
skeletal myoblast cells NGf6ZXhEgXSxdH;4bYNqfHliYYPzZZk> NF:xcFM4OiCqb4Xydy=> M4jPS2RPTEl8IFP5eI91d3irY3n0fUBqdiCYaYTyc{whPzJiaH;1dkwhcW5icnH0JJNs\WyndHHsJI16d2KuYYP0JINmdGy|LDDJR|UxRTF{LkC1{txONg>? M2DDWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU5PC9pPlPoSW1DVDxxYU6=
MT2 NWL6b5hpS3m2b4TvfIlkcXS7IHHzd4F6 NVjVcYFvPSCmYYnz NXWxUmpZS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVVR{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB3IHThfZMh[nliWGTUJIF{e2G7LDDDR|UxRTF7LkJOwG0v M3TpO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU5PC9pPlPoSW1DVDxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Growth inhibition assay
Cell viability; 

PubMed: 22271897     


Histograms show the proliferation of RPMI, LP1, U266, OPM2 and MM1S cell lines treated with nelfinavir (1 μM to 10 μM).

22271897
Western blot
p-eIF2Aα / eIF2Aα / ATF4 / GRP78 / CHOP ; 

PubMed: 21697087     


U251 cells were mock-treated (0) or treated with Nelfinavir (NFV) (20 μm) for 4 or 24 h then whole cell lysates were immunoblotted for P-eIF2α, eIF2α, ATF4, GRP78, CHOP, and Ran, as indicated. 

SOD1 / SOD2 / catalase ; 

PubMed: 27280849     


Protein lysates derived from MDA-MB231, MCF-7, or breast epithelial cells, treated with 10 μM nelfinavir for the indicated time points, were immunoblotted with anti- SOD1, anti-SOD2 and anti-catalase. Beta-actin was used as loading control. 

PAX3 / MITF ; 

PubMed: 26977879     


Western blot of the indicated cell lines treated with 10 μM nelfinavir for 24 hr for PAX3, MITF, and ERK2.

21697087 27280849 26977879
Immunofluorescence
LC3 / p62 ; 

PubMed: 27330277     


Immunofluorescence of cells treated with control 0.1% dimethyl sulfoxide or 10 μM nelfinavir for 24 hours. Scale bars indicate 10 μm and apply to all images. Punctate LC3 were quantified as autophagosomes and punctate p62 were quantified as sequestosomes, **P<0.01, ***P<0.001. (C and D) Western blot of lysates prepared following 24 hours of drug treatment. Nelfinavir was used at 10 μM and the autophagy clearance inhibitor chloroquine at 10 μM. 

PAX3 / MITF ; 

PubMed: 26977879     


Immunofluorescence analysis for PAX3 and MITF in WM266-4 cells left untreated or treated with 10 μM nelfinavir for 24 hr; scale bars, 50 μm.

p-SMAD2 ; 

PubMed: 26977879     


Immunofluorescence analysis for phospho-SMAD2 in melanoma cells treated with nelfinavir; scale bars, 10 μm

27330277 26977879
In vivo In vivo studies indicate that AG1343 is well absorbed orally in a variety of species and possesses favorable pharmacokinetic properties in humans. Initial investigation of the compound in fed rats indicated an oral bioavailability of 43%. In contrast, the oral bioavailability was significantly reduced in fasted rats to 29%. Nelfinavir demonstrated significant oral bioavailabilability across a range of species including dogs (47%), marmosets (17%), and cynomolgus monkeys (26%). It has a long plasma half-life after oral dosing that is likely due to a combination of slow dissolution and absorption. nelfinavir is well absorbed in humans particularly when administered with food and possesses minimal and easily managed side effects[1].

Protocol

Animal Research:

[1]

- Collapse
  • Animal Models: Sprague-Dawley rats
  • Dosages: 12.5 to 25 mg/kg (i.v); 25 to 50 mg/kg (oral)
  • Administration: i.v and oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (150.62 mM)
Water Insoluble
Ethanol ''100 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 663.89
Formula

C33H49N3O7S2

CAS No. 159989-65-8
Storage powder
in solvent
Synonyms Viracept
Smiles CC1=C(C=CC=C1O)C(=O)NC(CSC2=CC=CC=C2)C(CN3CC4CCCCC4CC3C(=O)NC(C)(C)C)O.CS(=O)(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

HIV Protease Signaling Pathway Map

Related HIV Protease Products

Tags: buy Nelfinavir (AG 1343) Mesylate | Nelfinavir (AG 1343) Mesylate supplier | purchase Nelfinavir (AG 1343) Mesylate | Nelfinavir (AG 1343) Mesylate cost | Nelfinavir (AG 1343) Mesylate manufacturer | order Nelfinavir (AG 1343) Mesylate | Nelfinavir (AG 1343) Mesylate distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID