Nelfinavir (AG 1343) Mesylate

Catalog No.S4282 Synonyms: Viracept

For research use only.

Nelfinavir Mesylate (Viracept, AG1343) is a potent HIV protease inhibitor with Ki of 2 nM.

Nelfinavir (AG 1343) Mesylate Chemical Structure

CAS No. 159989-65-8

Selleck's Nelfinavir (AG 1343) Mesylate has been cited by 15 publications

Purity & Quality Control

Choose Selective HIV Protease Inhibitors

Other HIV Protease Products

Biological Activity

Description Nelfinavir Mesylate (Viracept, AG1343) is a potent HIV protease inhibitor with Ki of 2 nM.
Targets
HIV protease [1]
(Cell-free assay)
2 nM(Ki)
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa Mk\USpVv[3Srb36gZZN{[Xl? NGL0WYRVWF:WUlHOV3BQWlSHUkqgbY5pcWKrdHnvckBw\iCWRVGgeZB1[WunIDjUSWE7KDFyIIXNLUBqdiCRQ2SxMYV5eHKnc4PpcochUGWOYTDj[YxteyxiSVO1NF0zOS56zszNMi=> NV7ZXWxzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2PFE{PzhpPkGwOlgyOzd6PD;hQi=>
Caco-2 NHPLblNHfW6ldHnvckBie3OjeR?= NEXGdWlVWF:WUlHOV3BQWlSHUkqgbY5pcWKrdHnvckBw\iCGYYXuc5J2[mmlaX6geJJidnOncHn0bIVtcWGuIITyZY5{eG:{dDCoZoF{[WxidH:gZZBq[2GuKTCoSIF2dm:{dXLpZ4lvQiB3IIXNLUBqdiCFYXPvMVIh[2WubIOsJGlEPTB;MT60{txONg>? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODh{MEGzO{c,OTB6MkCxN|c9N2F-
MT-4 MWfBcpRqfmm{YXygZZN{[Xl? M4DsemFvfGlvSFnWMVEh[WO2aY\peJkh[WejaX7zeEBYcWymIIT5dIUhfmm{dYOgbY4hVVRvNDDj[YxteyxiRVS1NF0xNjB{zszNMi=> NI\oTVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW0N|Y4Pyd-MUG1OFM3Pzd:L3G+
MT-4 NHnVUFNCdnSrdnnyZYwh[XO|YYm= NVz5[WRxSW62aT3ITXYuOSCjY4Tpeol1gSCjZ3HpcpN1KG23dHHueEBJUVZvMTDpckBOXC12IHPlcIx{KCivdYTheIlwdiC|ZXzlZ5Rm\CC5aYToJINwdXCxdX7kJFEqNCCHREWwQVAvODgQvF2u Mon2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF3NEO2O|coRjFzNUSzOlc4RC:jPh?=
MT-4 M4LCZWFvfGm4aYLhcEBie3OjeR?= Ml3BRY51cS2KSW[tNUBi[3Srdnn0fUBi\2GrboP0JI12fGGwdDDITXYuOSCrbjDNWE01KGOnbHzzJEhufXSjdHnvckB{\WynY4Tl[EB4cXSqIILpeI9v[X[rcjmsJGVFPTB;MD6xNe69VS5? NG\rcmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW0N|Y4Pyd-MUG1OFM3Pzd:L3G+
MT-4 MUXBcpRqfmm{YXygZZN{[Xl? NEXzNHNCdnSrLVjJWk0yKGGldHn2bZR6KGGpYXnud5QhdXW2YX70JGhKXi1zIHnuJG1VNTRiY3XscJMhMG23dHH0bY9vKHOnbHXjeIVlKHerdHigd4FyfWmwYY\pdkktKEWGNUC9NE4yOs7:TT6= MluzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF3NEO2O|coRjFzNUSzOlc4RC:jPh?=
MT-4 MnTkRY51cX[rcnHsJIF{e2G7 MUPBcpRqNUiLVj2xJIFkfGm4aYT5JIFo[Wmwc4SgcZV1[W62IFjJWk0yKGmwIF3UMVQh[2WubIOgLI12fGG2aX;uJJNmdGWldHXkJJdqfGhibnXs[olv[X[rcjmsJGVFPTB;Mj60OO69VS5? NGHS[GU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW0N|Y4Pyd-MUG1OFM3Pzd:L3G+
LLC-PK1 MWrGeY5kfGmxbjDhd5NigQ>? NXnpdYNSUW6qaXLpeIlwdiCxZjDQMYdtgWOxcILveIVqdixibX;1d4UhVC2vZIKxZUBmgHC{ZYPz[YQhcW5iTFzDMXBMOSCncHn0bIVtcWGuIHPlcIx{KHW|aX7nJINidGOnaX6tRW0heG:uYYLpd4F1cW:wIHHzd4F6NCCLQ{WwQVIvO87:TT6= NWrrVZNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2PVk{QDlpPkGyOlk6Ozh7PD;hQi=>
LLC-PK1 NXLqc3kyTnWwY4Tpc44h[XO|YYm= NED5dXJVWF:WUlHOV3BQWlSHUkqgbY5pcWKrdHnvckBw\iCFYXzj[YlvNUGPIHXm[ox2gCCrbjDN[JIy[S2neIDy[ZN{cW6pIFzMR{1RUzFiY3XscJMtKEmFNUC9Nk4{|ryPLh?= M3q1WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{Nkm5N|g6Lz5zMk[5PVM5QTxxYU6=
LLC-PK1 NUH3[5JVTnWwY4Tpc44h[XO|YYm= MYHJcohq[mm2aX;uJI9nKFBvZ3z5Z49xem:2ZXnuMEBpfW2jbjDMMW1FWjFiZYjwdoV{e2WmIHnuJGxNSy2SS{Gg[ZBqfGinbHnhcEBk\WyuczD1d4lv\yClYXzj[YlvNUGPIIDvcIFzcXOjdHnvckBie3OjeTygTWM2OD1|LkVOwG0v MmHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4OUmzPFkoRjF{Nkm5N|g6RC:jPh?=
LLC-PK1 MojySpVv[3Srb36gZZN{[Xl? NX20NVNUXFChVGLBUnNRV1KWRWK6JIlvcGmkaYTpc44hd2ZiQ3HsZ4Vqdi2DTTDl[oZtfXhiaX6gUWRTOS2neIDy[ZN{cW6pIFzMR{1RUzFiY3XscJMtKEmFNUC9N{41|ryPLh?= M{PnblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{Nkm5N|g6Lz5zMk[5PVM5QTxxYU6=
HEK NHW4UGxHfW6ldHnvckBie3OjeR?= NYnpepp5XFChVGLBUnNRV1KWRWK6JIlv[3KnYYPlJIlvKG2rdH;4ZY51em:wZTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBDS1KSLXX4dJJme3OrbnegTGVMKGOnbHzzMEBKSzVyPUGyMlXPxE1w M1P6dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MEC3NVAzLz5zNUCwO|ExOjxxYU6=
CEM-SS NX3MdWxwSW62aY\pdoFtKGG|c3H5 M1jKOVYh\GG7cx?= Mm[xRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSB|QjDpcoZm[3SnZDDpckBpfW2jbjDDSW0uW1NiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXK3cz3pcoR2[2WmIHP5eI9x[XSqaXOg[YZn\WO2IHHmeIVzKDZiZHH5d{BjgSCPVGOgZZN{[XluIFXEOVA:OC5yM988UU4> MnOwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV{MUeyPFEoRjF3MkG3NlgyRC:jPh?=
HOG.R5 MUPBcpRqfmm{YXygZZN{[Xl? NXH4XYhQPCCmYYnz MXjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKDOEIHnu[oVkfGWmIHnuJIh2dWGwIFjPS{5TPSClZXzsd{Bi\nSncjC0JIRigXNuIFnDOVA:OC5yOd88UU4> NFWyNHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUKxO|I5OSd-MUWyNVczQDF:L3G+
MT-4 MmHwSpVv[3Srb36gZZN{[Xl? MYXF[oZm[3SrdnWg[I9{\SCxZjDjc41xd3WwZDDy[ZF2cXKnZDD0c{BqdmirYnn0JJJmeGyrY3H0bY9vKG:oIHj1cYFvKGmvbYXuc4Rm\mmlaXXuZ5khfmm{dYOgeJlx\SBzIHnuJG1VNTRiY3XscJMtKEWGNUC9NE4xOs7:TT6= NGS1eoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUWzO|M2OCd-MUW1N|c{PTB:L3G+
MT4 NGXRZ21CdnSrdnnyZYwh[XO|YYm= NV3XWm5CSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDIXGIzKGmwIF3UOEBk\WyuczygTWM2OD1yLkOy{txONg>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR3OEWwOUc,OTZ2NUi1NFU9N2F-
MT4 NGLaRo9CdnSrdnnyZYwh[XO|YYm= M1;4TmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiRWCxN{BqdiCPVESgZ4VtdHNuIFnDOVA:OC52Nd88UU4> M3;TUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEW4OVA2Lz5zNkS1PFUxPTxxYU6=
PHA-PBMC Ml3xRY51cX[rcnHsJIF{e2G7 NUK0W3R2SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDCRU1NKGm|b3zheIUhcW5iaIXtZY4hWEiDLWDCUWMh[2WubIOsJGlEPTB;MD6wNFfPxE1w MnqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MUO3NVQoRjF4OUGzO|E1RC:jPh?=
MT2 NWqzfJc6SW62aY\pdoFtKGG|c3H5 M{j4dWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTFHJJIl{d2yjdHWgbY4hcHWvYX6gUXQzKGOnbHzzMEBKSzVyPUCuNFHPxE1w M3j3fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUGzO|E1Lz5zNkmxN|cyPDxxYU6=
PHA-PBMC MWnBcpRqfmm{YXygZZN{[Xl? NI\C[2NCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIFzBTUBqe2:uYYTlJIlvKGi3bXHuJHBJSS2SQl3DJINmdGy|LDDJR|UxRTBwMEG0{txONg>? M3;hblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUGzO|E1Lz5zNkmxN|cyPDxxYU6=
MT2 M3ryWmFvfGm4aYLhcEBie3OjeR?= MnPmRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOiCHSF:gbZNwdGG2ZTDpckBpfW2jbjDNWFIh[2WubIOsJGlEPTB;MD6wNu69VS5? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjlzM{exOEc,OTZ7MUO3NVQ9N2F-
PHA-PBMC M1e2TWFvfGm4aYLhcEBie3OjeR?= MnXnRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgcZVtfGliZIL1[{1z\XOrc4ThcpQhUEmYMTDFWEB3[XKrYX70JIlvKGi3bXHuJHBJSS2SQl3DJINmdGy|LDDJR|UxRTBwMEOy{txONg>? NH7zWmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmxN|cyPCd-MU[5NVM4OTR:L3G+
PHA-PBMC NGfMZndCdnSrdnnyZYwh[XO|YYm= M{fD[2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JI12dHSrIHTyeYcuemW|aYP0ZY51KEiLVkGgS{B3[XKrYX70JIlvKGi3bXHuJHBJSS2SQl3DJINmdGy|LDDJR|UxRTBwMUhOwG0v NV\VTpZ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NVM4OTRpPkG2PVE{PzF2PD;hQi=>
PHA-PBMC M{CwNGFvfGm4aYLhcEBie3OjeR?= MXXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDteYx1cSCmcoXnMZJme2m|dHHueEBJUVZzIFOgeoFzcWGwdDDpckBpfW2jbjDQTGEuWEKPQzDj[YxteyxiSVO1NF0xNjNzzszNMi=> MoP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MUO3NVQoRjF4OUGzO|E1RC:jPh?=
MT2 M3z0SWFvfGm4aYLhcEBie3OjeR?= M3zibmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjJiRVjPJIlvKE2WMjDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHB{NDDHZYcheHKxdHXpckBmgHC{ZYPzbY9vKGK7IF3UWEBie3OjeTygSWM2OD1yLkCz{txONg>? M4DqVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{exPFEyLz5zN{O3NVgyOTxxYU6=
MT2 MXLBcpRqfmm{YXygZZN{[Xl? NG[xc4NCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIFzBTUBqdiCPVEKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyMkSgS4FoKHC{b4TlbY4h\XiycnXzd4lwdiCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wN|LPxE1w MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN5MUixNUc,OTd|N{G4NVE9N2F-
MT4 MonmRY51cX[rcnHsJIF{e2G7 MVTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKE6OND2zJIlvKE2WNDDj[YxteyCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wN|PPxE1w MnvvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd|N{G4NVEoRjF5M{exPFEyRC:jPh?=
MT4 M3WydWFvfGm4aYLhcEBie3OjeR?= MnP6RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCJUly5PFA3PXB{MDDpckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxOjRiR3HnJJBzd3SnaX6g[ZhxemW|c3nvckBjgSCPVGSgZZN{[XluIFXDOVA:OC5yON88UU4> NX\uXY51RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT4 NFrIWVRCdnSrdnnyZYwh[XO|YYm= M2HOfmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJJqfG:wYY\pdk1z\XOrc4ThcpQhUEmYMTDpckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxOjRiR3HnJJBzd3SnaX6g[ZhxemW|c3nvckBjgSCPVGSgZZN{[XluIFXDOVA:OC5{Md88UU4> NYjzb29ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT4 NIfCR|hCdnSrdnnyZYwh[XO|YYm= M3i1UmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIFueHKnbnH2bZIuemW|aYP0ZY51KEiLVkGgbY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheDJ2IFfh[{Bxem:2ZXnuJIV5eHKnc4Ppc44h[nliTWTUJIF{e2G7LDDFR|UxRTBwMkNOwG0v M1jlU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{exPFEyLz5zN{O3NVgyOTxxYU6=
MT2 NFXqTpFCdnSrdnnyZYwh[XO|YYm= NVftZYU5SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMjDSU2QhcW5iTWSyJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZicEK0JGdi\yCycn;0[YlvKGW6cILld5Nqd25iYomgUXRVKGG|c3H5MEBGSzVyPUCuNlTPxE1w Mkm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd|N{G4NVEoRjF5M{exPFEyRC:jPh?=
MT4 NYnONJJGSW62aY\pdoFtKGG|c3H5 NHPtXlJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBifGG8YX7heolzNXKnc3nzeIFvfCCKSW[xJIlvKE2WNDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHB{NDDHZYcheHKxdHXpckBmgHC{ZYPzbY9vKGK7IF3UWEBie3OjeTygSWM2OD1yLkK3{txONg>? NYqyO4lIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT4 M4njTmFvfGm4aYLhcEBie3OjeR?= MWrBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKEeUTEm4NFY2eDNyIHnuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJAzPCCJYXegdJJwfGWrbjDlfJBz\XO|aX;uJIJ6KE2WVDDhd5NigSxiRVO1NF0xNjJ5zszNMi=> NHPaV4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{O3NVgyOSd-MUezO|E5OTF:L3G+
MT4 MV7BcpRqfmm{YXygZZN{[Xl? NIX6XodCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIFfSUFk5ODZ3cESwJIlvKE2WNDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHB{NDDHZYcheHKxdHXpckBmgHC{ZYPzbY9vKGK7IF3UWEBie3OjeTygSWM2OD1yLkO0{txONg>? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN5MUixNUc,OTd|N{G4NVE9N2F-
MT4 MlTCRY51cX[rcnHsJIF{e2G7 NWr1fYw5SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhcWSrbnH2bZIuemW|aYP0ZY51KEiLVkGgbY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheDJ2IFfh[{Bxem:2ZXnuJIV5eHKnc4Ppc44h[nliTWTUJIF{e2G7LDDFR|UxRTBwNEhOwG0v NEXaUmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{O3NVgyOSd-MUezO|E5OTF:L3G+
MT4 MWDBcpRqfmm{YXygZZN{[Xl? M2[zcmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJNieXWrbnH2bZIuemW|aYP0ZY51KEiLVkGgbY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheDJ2IFfh[{Bxem:2ZXnuJIV5eHKnc4Ppc44h[nliTWTUJIF{e2G7LDDFR|UxRTBwNElOwG0v NWTkVoxKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT4 M2jGSWFvfGm4aYLhcEBie3OjeR?= NEDwO5RCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBtd3CrbnH2bZIuemW|aYP0ZY51KEiLVkGgbY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheDJ2IFfh[{Bxem:2ZXnuJIV5eHKnc4Ppc44h[nliTWTUJIF{e2G7LDDFR|UxRTBwNEpOwG0v NVi0bJN3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT2 MnKyR5l1d3SxeHnjbZR6KGG|c3H5 M2HMNmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1VOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJAzPCCJYXegdJJwfGWrbjDlfJBz\XO|aX;uJIJ6KE2WVDDhd5NigSxiQ1O1NF05NjIQvF2u NW[5flRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezO|E5OTFpPkG3N|cyQDFzPD;hQi=>
MT4 NF\KdVlCdnSrdnnyZYwh[XO|YYm= M1zvfWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY4hVVR2IHPlcIx{KGK7IF3UWEBie3OjeTygSWM2OD1yLkCxOu69VS5? M2HVZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUO3OlI5Lz5zN{WzO|YzQDxxYU6=
MT4 MnzPR5l1d3SxeHnjbZR6KGG|c3H5 MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBOXDRiY3XscJMh[nliTWTUJIF{e2G7LDDMR|UxRTFzzszNMi=> NETyPWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WzO|YzQCd-MUe1N|c3Ojh:L3G+
MT4 NYi3TolDSW62aY\pdoFtKGG|c3H5 NFrnR2Q2KGSjeYO= NXPWUHJxSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDOUFQuOyCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZYZ1\XJiNTDkZZl{KGK7IF3UWEBie3OjeTygSWM2OD1yLkCxPO69VS5? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV5Nki0PEc,OTd3N{[4OFg9N2F-
MT4 NEnlUHdCdnSrdnnyZYwh[XO|YYm= NUjJR|FzPSCmYYnz NVHFN|YzSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMjDNV{Bqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYX\0[ZIhPSCmYYnzJIJ6KE2WVDDhd5NigSxiRVO1NF0xNjB2ON88UU4> NUHVfGJURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1O|Y5PDhpPkG3OVc3QDR6PD;hQi=>
CEM MVnBcpRqfmm{YXygZZN{[Xl? M2PnPVch\GG7cx?= NF;kVYFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZ{IGLPSEB4cXSqIIDyc5Rm[XOnIFexO24wXjR5QTDteZRifGmxbjDpcoZm[3SnZDDpckBpfW2jbjDDSW0h[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJ2eyCycn;keYN1cW:wIHHmeIVzKDdiZHH5d{BjgSCOZX70bU1TXCCjY4Tpeol1gSCjc4PhfUwhTUN3ME2wMlHPxE1w MkPGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3N{[4OFgoRjF5NUe2PFQ5RC:jPh?=
CEM MULBcpRqfmm{YXygZZN{[Xl? M3vQWFch\GG7cx?= NWW2PXc3SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMjDSU2Qhf2m2aDDwdo91\WG|ZTDWOFdCKG23dHH0bY9vKGmwZnXjeIVlKGmwIHj1cYFvKEOHTTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzJJBzd2S3Y4Tpc44h[W[2ZYKgO{Bl[Xm|IHL5JGxmdnSrLWLUJIFkfGm4aYT5JIF{e2G7LDDFR|UxRTBwMUe3{txONg>? Mki3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3N{[4OFgoRjF5NUe2PFQ5RC:jPh?=
CEM M2OwfWFvfGm4aYLhcEBie3OjeR?= Mn\VO{Bl[Xm| NXPYdnNrSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMjDSU2Qhf2m2aDDwdo91\WG|ZTDHNVdPKG23dHH0bY9vKGmwZnXjeIVlKGmwIHj1cYFvKEOHTTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzJJBzd2S3Y4Tpc44h[W[2ZYKgO{Bl[Xm|IHL5JGxmdnSrLWLUJIFkfGm4aYT5JIF{e2G7LDDFR|UxRTBwMUm5{txONg>? MkXKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3N{[4OFgoRjF5NUe2PFQ5RC:jPh?=
CEM MU\BcpRqfmm{YXygZZN{[Xl? NFHEVmE4KGSjeYO= NV3EbZgzSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5Qhf2muZDD0fZBmKEiLVkKgVm9FKGmwZnXjeIVlKGmwIHj1cYFvKEOHTTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzJJBzd2S3Y4Tpc44h[W[2ZYKgO{Bl[Xm|IHL5JGxmdnSrLWLUJIFkfGm4aYT5JIF{e2G7LDDFR|UxRTBwMkix{txONg>? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV5Nki0PEc,OTd3N{[4OFg9N2F-
HEK293 MmGzRY51cX[rcnHsJIF{e2G7 NYL4S4RsPDhiaILz NXHNR2s{SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5Qhf2muZDD0fZBmKEiLVkGgUmw1NTNiaX6gTGVMOjl|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBz\XCuaXPheIlwdi2mZX\m[YN1cX[nIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\S2kYYPl[EBxcGWwb4T5dIlkKGG|c3H5MEBGSzVyPUCuNFA2QM7:TT6= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ|OE[5OEc,OTd4M{i2PVQ9N2F-
MT4 NEjsdoVCdnSrdnnyZYwh[XO|YYm= NHXv[Wc3KGSjeYO= MYrBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgTGlXOSCQTEStN{Bqdm[nY4Tl[EBqdiCPVESgZ4VtdHNiYX\0[ZIhPiCmYYnzJIJ6KE2WVDDhd5NigSxiRVO1NF0xNjB{Od88UU4> NX\sPY5[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2N|g3QTRpPkG3OlM5Pjl2PD;hQi=>
MT4 Moq0R5l1d3SxeHnjbZR6KGG|c3H5 MUK2JIRigXN? MnjWR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXQ1KGOnbHzzJIFnfGW{IE[g[IF6eyCkeTDNWHQh[XO|YYmsJGNEPTB;ON88UU4> M320elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkO4Olk1Lz5zN{[zPFY6PDxxYU6=
HEK293 MVXGeY5kfGmxbjDhd5NigQ>? NX75RlRVOC5zMjD1US=> M{\YcWlvcGmkaYTpc44hd2ZiSFnWNUBPVDRvMzDwdo91\WmwczDpcoNwenCxcnH0bY9vKGmwdH:geolzcW:wczD0doFve2[nY4Tl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhfW6ycn;j[ZN{\WRiZ4CxOlAhTW64IIDyc5RmcW5iYYSgNE4yOiC3TTDifUBY\XO2ZYLuJIJtd3R? MlTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NE[0NVAoRjF5NkS2OFExRC:jPh?=
HEK293 NG\0SmdHfW6ldHnvckBie3OjeR?= MUiwMlEzKHWP NWHUU|hYUW6qaXLpeIlwdiCxZjDITXYyKE6OND2zJJBzd3SnaX7zJIlv[2:{cH;yZZRqd25iaX70c{B3cXKrb37zJJRz[W6|ZnXjeIVlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCjYnXydoFvfCCHbo[gdJJwfGWrbjDheEAxNjF{IIXNJIJ6KFenc4Tldo4h[myxdB?= NFT1S5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[0OlQyOCd-MUe2OFY1OTB:L3G+
MDCK2 M4TrOWlvcGmkaYTpc44hd2ZiaIXtZY4> NXLKeZZHUW6qaXLpeIlwdiCxZjDoeY1idiCPRGKxMYRmeGWwZHXueEBi[2O3bYXsZZRqd25ib3[gZ4Ft[2Wrbj3BUUBmgHC{ZYPz[YQhcW5iTVTDT|Ih[2WubIOsJGlEPTB;MUmuPe69VS5? NGLhUI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[2OFMzPyd-MUe2OlQ{Ojd:L3G+
MDR1 NX30OGxETnWwY4Tpc44h[XO|YYm= M2XIfFIxKHWP MU\E[YNz\WG|ZTDpckBRNWeueXPvdJJwfGWrbj3t[YRq[XSnZDD0[Y5w\m:4aYKg[Il{d3C{b4jpcEBnfW2jcnH0[UBm\m[udYigbY4hVUSFS{Kg[ZhxemW|c3nu[{BpfW2jbjDNSHIyKGOnbHzzJIF1KDJyIIXN NW\Ub3M1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OlQ{OjdpPkG3OlY1OzJ5PD;hQi=>
CEM-SS MVXBcpRqfmm{YXygZZN{[Xl? NHK0S4Y2KGSjeYO= M1n3[WFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTFHJJIlv\mWldHXkJIlvKGi3bXHuJGNGVS2VUzDj[YxteyCjc4Pld5Nm\CCjczDy[ZZmenOnIITyZY5{[3KrcIThd4Uh[WO2aY\peJkh[W[2ZYKgOUBl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlAxOs7:TT6= NETIW2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m1NFk2PSd-MUe5OVA6PTV:L3G+
MOLT-4/3B MojqRY51cX[rcnHsJIF{e2G7 MYK0JIRigXN? NGL0UnhCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKE2RTGStOE8{SiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJI1ifHW{ZTD2bZJ2eyC{ZXzlZZNmKG2nYYP1doVlKGGodHXyJFQh\GG7czDv[kBqdm[nY4Tpc44h[nliUmSgZZN{[XluIFXDOVA:OC5yMkpOwG0v MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDNzNkWyNUc,OTh|MU[1NlE9N2F-
MT4 NGrEd|ZCdnSrdnnyZYwh[XO|YYm= MVTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKDOEIHnuJIh2dWGwIF3UOEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtNWmwZIXj[YQh[3m2b4DheIhq[yCnZn\lZ5Qh[nliTWTUJI1mfGixZDygSWM2OD1yLkCxOu69VS5? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR{NkG5OUc,OTh2Mk[xPVU9N2F-
MT4 NEfTdIREgXSxdH;4bYNqfHliYYPzZZk> M2XROmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1VPCClZXzsd{BjgSCPVGSgZZN{[XluIFPDOVA:OTIQvF2u MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR{NkG5OUc,OTh2Mk[xPVU9N2F-
MT4 MXrBcpRqemW2cn;2bZJidCCjc4PhfS=> MmjsRY51cXKndILveolz[WxiYXP0bZZqfHliYXfhbY5{fCCKdX3hckBqdW23bn;k[YZq[2mnbnP5JJZqenW|IEGgUmw1NjNiaX7m[YN1\WRiaX6gbJVu[W5iTWS0JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBjgSCPVGSgZZN{[XluIFXDOVA:OC5yMkROwG0v MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV2MUeyOkc,OTh3NEG3NlY9N2F-
MT4 MmXhRY51cXKndILveolz[WxiYYPzZZk> MkPHRY51cXKndILveolz[WxiYXP0bZZqfHliYXfhbY5{fCCKdX3hckBqdW23bn;k[YZq[2mnbnP5JJZqenW|IEGgN2IhcW6oZXP0[YQhcW5iaIXtZY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{dYOtbY5lfWOnZDDjfZRweGG2aHnjJIVn\mWldDDifUBOXFRiYYPzZZktKEWFNUC9NE4xOjkQvF2u MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV2MUeyOkc,OTh3NEG3NlY9N2F-
MT4 MYXBcpRqfmm{YXygZZN{[Xl? MVLBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDS[ZZmenOnIITyZY5{[3KrcIThd4UhcW6qaXLpeI9zNXKnc3nzeIFvfCCKdX3hckBqdW23bn;k[YZq[2mnbnP5JJZqenW|IEGgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNvaX7keYNm\CCleYTvdIF1cGmlIHXm[oVkfCCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wNlnPxE1w Mk\RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NEG3NlYoRjF6NUSxO|I3RC:jPh?=
MT4 NH63O2dCdnSrdnnyZYwh[XO|YYm= MV\BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDuc44hdnWlbHXvd4ll\SC{ZY\ldpNmKHS{YX7zZ5JqeHSjc3WgbY5pcWKrdH;yMZJme2m|dHHueEBJfW2jbjDpcY12dm:mZX\pZ4lmdmO7II\pdpV{KDFiaX7m[YN1\WRiaX6gbJVu[W5iTWS0JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBjgSCPVGSgZZN{[XluIFXDOVA:OC5yM{ZOwG0v NV3PVYRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OFE4OjZpPkG4OVQyPzJ4PD;hQi=>
MT4 MkHJRY51cXKndILveolz[WxiYYPzZZk> NGPLO3VCdnSrcnX0do93cXKjbDDhZ5Rqfmm2eTDh[4FqdnO2IFj1cYFvKGmvbYXuc4Rm\mmlaXXuZ5khfmm{dYOgNkBGUE9iaX7m[YN1\WRiaX6gbJVu[W5iTWS0JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBjgSCPVGSgZZN{[XluIFXDOVA:OC5yNEpOwG0v NIDkcFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW0NVczPid-MUi1OFE4OjZ:L3G+
MT4 NHT2PFFCdnSrcnX0do93cXKjbDDhd5NigQ>? MVHBcpRqemW2cn;2bZJidCCjY4Tpeol1gSCjZ3HpcpN1KEi3bXHuJIludXWwb3Tl[olkcWWwY4mgeolzfXNiMjDSU2QhcW6oZXP0[YQhcW5iaIXtZY4hVVR2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{dYOtbY5lfWOnZDDjfZRweGG2aHnjJIVn\mWldDDifUBOXFRiYYPzZZktKEWFNUC9NE4xPc7:TT6= NX\OW|VTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1OFE4OjZpPkG4OVQyPzJ4PD;hQi=>
MT4 M4j2TGFvfGm{ZYTyc5ZqemGuIHHzd4F6 MleyRY51cXKndILveolz[WxiYXP0bZZqfHliYXfhbY5{fCCVaX3pZY4hcW2vdX7v[IVncWOrZX7jfUB3cXK3czDNRWMhOjVzIHnu[oVkfGWmIHnuJIh2dWGwIF3UOEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdpV{NWmwZIXj[YQh[3m2b4DheIhq[yCnZn\lZ5Qh[nliTWTUJIF{e2G7LDDFR|UxRTBwMEZOwG0v Mnm0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NEG3NlYoRjF6NUSxO|I3RC:jPh?=
MT4 MkTMRY51cX[rcnHsJIF{e2G7 NXn1WoV{SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhWHKxdHXhd4UhcW6qaXLpeI9zNXKnc3nzeIFvfCCKdX3hckBqdW23bn;k[YZq[2mnbnP5JJZqenW|IEGgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNvaX7keYNm\CCleYTvdIF1cGmlIHXm[oVkfCCkeTDNWHQh[XO|YYmsJGVEPTB;MD6xO:69VS5? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV2MUeyOkc,OTh3NEG3NlY9N2F-
MT4 NVr0[4Y2SW62aY\pdoFtKGG|c3H5 Moq2OUB2VQ>? NYX3eJdbSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDOUFQuOyCqYYLic5JqdmdiTEGwSk9NOzOIL120OmkwUTR5Vj;ROVhGN1Z6MlmvTVg1Xi:LOEXWJIFucW6xIHHjbYQhe3Wkc4TpeJV1cW:wIHnuJJBzd3SnYYPlJIVv[2:maX7nJJJm\2mxbjDpcoZm[3SnZDDpckBpfW2jbjDNWFQh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDwNlQhT2GpIIDyc5RmcW5icILv[JVkfGmxbjDz[Yxm[3SnZDDheEA2KHWPIH;mJGdTVC1yMkCzNUBjgSCHLDDFR|UxRTBwMEGx{txONg>? M1;4V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUW1OVE5Lz5zOEm1OVUyQDxxYU6=
MT4 NW[5V|hwSW62aY\pdoFtKGG|c3H5 MkjXRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQTEStN{Bqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxOjRiZ3HnJJBzd3SnaX6gdJJw\HWldHnvckBjgSCHTFnTRUwhTUN3ME2wMlAyQM7:TT6= Mn;sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7NUW1NVgoRjF6OUW1OVE5RC:jPh?=
MT2 MWLBcpRqfmm{YXygZZN{[Xl? NXTYR5NoPyCmYYnz NWfm[YZuSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDMRWkhcW6oZXP0[YQhcW5iaIXtZY4hVVR{IHPlcIx{KGGodHXyJFch\GG7czDifUBOXFRiYYPzZZktKEWFNUC9NE4xOjQQvF2u M{\aTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUW1OVE5Lz5zOEm1OVUyQDxxYU6=
MT4 NETTN5FCdnSrdnnyZYwh[XO|YYm= M{n1Z|UhfU1? MkixRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQTEStN{Bp[XKkb4LpcochVDFyRj;WN|JKN012NlmvTVU1VS9xQUexWk9KQDSYIHHtbY5wKGGlaXSgd5Vje3SrdIX0bY9vKGmwIIDyc5Rm[XOnIHXuZ49lcW6pIILl[4lwdiCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyMkSgS4FoKHC{b4TlbY4heHKxZIXjeIlwdiC|ZXzlZ5Rm\CCjdDC1JJVOKG:oIHHtdJJmdmG4aYKgZpkhTUyLU1GsJGVEPTB;MD6xO:69VS5? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl3NUWxPEc,OTh7NUW1NVg9N2F-
MT4 NGLmVYhCdnSrdnnyZYwh[XO|YYm= NVHsN4JnOSC3TR?= M330cmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTly0MVMhcGG{Yn;ybY5oKEx{M1mvT|Q{US:PNE\JM2k2OExxR{WxRU9CPzGYIHHtbY5wKGGlaXSgd5Vje3SrdIX0bY9vKGmwIIDyc5Rm[XOnIHXuZ49lcW6pIILl[4lwdiCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyMkSgS4FoKHC{b4TlbY4heHKxZIXjeIlwdiC|ZXzlZ5Rm\CCjdDCxJJVOKG:oIHH0ZZpidmG4aYKgZpkhTUyLU1GsJGVEPTB;MD6yNe69VS5? NUHVdo1uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi5OVU2OThpPkG4PVU2PTF6PD;hQi=>
MT4 MYPBcpRqfmm{YXygZZN{[Xl? NGT1U|c2KHWP MVfBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKE6OND2zJIhiemKxcnnu[{BOPD[LL2[4NmYwUTh2VjDhcYlvdyCjY3nkJJN2[nO2aYT1eIlwdiCrbjDwdo91\WG|ZTDlcoNw\GmwZzDy[Ydqd25iaX7m[YN1\WRiaX6gbJVu[W5iTWS0JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZicEK0JGdi\yCycn;0[YlvKHC{b3T1Z5Rqd25ic3Xs[YN1\WRiYYSgOUB2VSCxZjDybZRwdmG4aYKgZpkhTUyLU1GsJGVEPTB;MD6yOO69VS5? M1HiclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUW1OVE5Lz5zOEm1OVUyQDxxYU6=
MT4 MofHRY51cX[rcnHsJIF{e2G7 NXHiTotNPSC3TR?= NEDaSpBCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIF7MOE0{KGijcnLvdolv\yCOMUDJM2c1QFZxSUW0Wk9NQTCPIHHtbY5wKGGlaXSgd5Vje3SrdIX0bY9vKGmwIIDyc5Rm[XOnIHXuZ49lcW6pIILl[4lwdiCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyMkSgS4FoKHC{b4TlbY4heHKxZIXjeIlwdiC|ZXzlZ5Rm\CCjdDC1JJVOKG:oIIPhdZVqdmG4aYKgZpkhTUyLU1GsJGVEPTB;MD6zNu69VS5? M3izT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUW1OVE5Lz5zOEm1OVUyQDxxYU6=
MT4 NFXVdoRCdnSrdnnyZYwh[XO|YYm= MUmxJJVO NF[5UJFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIF7MOE0{KGijcnLvdolv\yCOMUDGM201PklxSUW0Wk9XQDKDIHHtbY5wKGGlaXSgd5Vje3SrdIX0bY9vKGmwIIDyc5Rm[XOnIHXuZ49lcW6pIILl[4lwdiCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyMkSgS4FoKHC{b4TlbY4heHKxZIXjeIlwdiC|ZXzlZ5Rm\CCjdDCxJJVOKG:oIFzvdIlv[X[rcjDifUBGVEmVQTygSWM2OD1yLkWx{txONg>? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl3NUWxPEc,OTh7NUW1NVg9N2F-
MT4 Ml;BRY51cX[rcnHsJIF{e2G7 NV;iR2RlPSC3TR?= M4faTWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTly0MVMhcGG{Yn;ybY5oKExzMF[vUFI1US:PNE\JM2w3O1BxQUexWk9IPzOVL2[4NnQh[W2rbn:gZYNq\CC|dXLzeIl1fXSrb36gbY4heHKxdHXhd4Uh\W6lb3TpcochemWpaX;uJIlv\mWldHXkJIlvKGi3bXHuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJAzPCCJYXegdJJwfGWrbjDwdo9lfWO2aX;uJJNmdGWldHXkJIF1KDVidV2gc4YhcW6maX7heolzKGK7IFXMTXNCNCCHQ{WwQVAvPjYQvF2u NVvLWHg{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi5OVU2OThpPkG4PVU2PTF6PD;hQi=>
HeLa MmXZRY51cX[rcnHsJIF{e2G7 NHjxdWo4OiCqcoO= NVTNWoRjSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDoZZJjd3KrbnegZ4Fxe2mmIFmyNFFXKG23dHHueEBxem:2ZXnuJIlv\mWldHXkJIlvKGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6geolz[WxiaX7m[YN1cW:wIHHmeIVzKDd{IHjyd{BjgSCkZYThMYdidGGldH;zbYRie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCxN:69VS5? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQThyNUW3NUc,OTl6MEW1O|E9N2F-
HeLa MWTBcpRqfmm{YXygZZN{[Xl? MVu3NkBpenN? M{DI[WFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRidInw[UBJUVZzIHnu[oVkfGWmIHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5idnnyZYwhcW6oZXP0bY9vKGGodHXyJFczKGi{czDifUBj\XSjLXfhcIFkfG:|aXThd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjBzNt88UU4> NHzOOVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUiwOVU4OSd-MUm4NFU2PzF:L3G+
HeLa MX7BcpRqfmm{YXygZZN{[Xl? NHTieog4OiCqcoO= NEjZcHNCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIHjhdoJwemmwZzDzdIFk\XJicHXweIll\SCDMW[gcZV1[W62IIDyc5RmcW5iaX7m[YN1\WRiaX6gbJVu[W5iSHXMZUBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiC4aYLhcEBqdm[nY4Tpc44h[W[2ZYKgO|IhcHK|IHL5JIJmfGFvZ3HsZYN1d3OrZHHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODN6zszNMi=> NX;KcYc1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4NFU2PzFpPkG5PFA2PTdzPD;hQi=>
cord blood mononuclear cells MUDBcpRqfmm{YXygZZN{[Xl? NWPu[3dRPDhiaILz M4\6OGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRidInw[UBJUVZzIIP1ZpR6eGViQzDpcoZm[3SnZDDpckBpfW2jbjDjc5JlKGKub3;kJI1wdm:wdXPs[YFzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiNEigbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlAxOTZ5zszNMi=> NH\w[os9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESwOFEzOyd-MkC0NFQyOjN:L3G+
cord blood mononuclear cells MYnBcpRqfmm{YXygZZN{[Xl? NVrpeo5nPDhiaILz MkTERY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sge4lt\CC2eYDlJGhKXjFic4XieJlx\SCFUl[wNn9CTyCrbn\lZ5Rm\CCrbjDoeY1idiClb4LkJIJtd2:mIH3vco9vfWOuZXHyJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25iYX\0[ZIhPDhiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCwOVfPxE1w NVj6N2dRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0NFQyOjNpPkKwOFA1OTJ|PD;hQi=>
cord blood mononuclear cells NFTyZWRCdnSrdnnyZYwh[XO|YYm= NXW4TGxPPDhiaILz NVrC[W4zSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5Qhf2muZDD0fZBmKEiLVkGgd5VjfHmyZTDCJIlv\mWldHXkJIlvKGi3bXHuJINwemRiYnzvc4QhdW:wb371Z4xm[XJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODB7ON88UU4> M3nvfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEC0NVI{Lz5{MESwOFEzOzxxYU6=
cord blood mononuclear cells NHXPdmlCdnSrdnnyZYwh[XO|YYm= MWS0PEBpenN? MnTDRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSC|dXL0fZBmKEOURkCyY2FIKGijcnLvdolv\yCycn;0[YF{\SCyb3z5cY9zeGirc32gZZQhUTN4IIDvd4l1cW:wIHHu[EBOPDZibYX0ZY51KGenbnWgbY5n\WO2ZXSgbY4hcHWvYX6gZ49z\CCkbH;v[EBud26xboXjcIVieiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xPTYQvF2u MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODRyNEGyN{c,OjB2MESxNlM9N2F-
cord blood mononuclear cells MUHBcpRqfmm{YXygZZN{[Xl? Mmi1OFghcHK| NWfpfnJFSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDzeYJ1gXCnIFPSSlAzZ0GJIHjhdoJwemmwZzDwdo91\WG|ZTDMN|NKNCCPNE\JMEBCPzGWIH31eIFvfCCpZX7lJIFv\CCyb3z5cY9zeGirc32gZZQhVTN4IIDvd4l1cW:wIHnu[oVkfGWmIHnuJIh2dWGwIHPvdoQh[myxb3SgcY9vd263Y3zlZZIh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB2ODDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSnLDDFR|UxRTBwMkCy{txONg>? Mmi5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MESxNlMoRjJyNEC0NVI{RC:jPh?=
cord blood mononuclear cells NXT6N|A{SW62aY\pdoFtKGG|c3H5 NYXJeZpEPDhiaILz MkHKRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSC|dXL0fZBmKENiaHHyZo9zcW6pIIDyc5Rm[XOnIFyyN2ktKE12NlmsJGw5QVRibYX0ZY51KGenbnWgZY5lKHCxbIntc5JxcGm|bTDheEBOOzZicH;zbZRqd25iaX7m[YN1\WRiaX6gbJVu[W5iY3;y[EBjdG:xZDDtc45wdnWlbHXhdkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHHmeIVzKDR6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhNCCHQ{WwQVAvOjd{zszNMi=> NHy3RpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESwOFEzOyd-MkC0NFQyOjN:L3G+
cord blood mononuclear cells MYnBcpRqfmm{YXygZZN{[Xl? NUXZe3RoPDhiaILz NV;hfGJzSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDzeYJ1gXCnIFKgbIFz[m:{aX7nJJBzd3SnYYPlJGQ{OE5uIF20OmktKEF5MWSgZY5lKFZ5N1mgcZV1[W62IHflcoUh[W6mIIDvcJlud3KyaHnzcUBifCCPM{[gdI9{cXSrb36gc4YheHKxdHXhd4UhcW6oZXP0[YQhcW5iaIXtZY4h[2:{ZDDicI9w\CCvb37vcpVkdGWjcjDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFQ5KGi{czDifUBtfWOrLDDFR|UxRTFwMUO5{txONg>? MoHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MESxNlMoRjJyNEC0NVI{RC:jPh?=
cord blood mononuclear cells Mly0RY51cX[rcnHsJIF{e2G7 MlHuOFghcHK| MUjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKHO3YoT5dIUhSiCqYYLic5JqdmdicILveIVie2ViREOwUkwhVDN|STygUVQ3USxiTki4SEBufXSjboSg[4Vv\SCjbnSgdI9tgW2xcoDobZNuKGG2IFmzOkBxd3OrdHnvckBqdm[nY4Tl[EBqdiCqdX3hckBkd3KmIHLsc49lKG2xbn;ueYNt\WG{IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXygdoVxdGmlYYTpc44h[W[2ZYKgOFghcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZItKEWFNUC9NU41PjQQvF2u M2K3VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEC0NVI{Lz5{MESwOFEzOzxxYU6=
cord blood mononuclear cells NWOyeWF7SW62aY\pdoFtKGG|c3H5 NYS2WZpuPDhiaILz NFXYWIZCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIIP1ZpR6eGViQzDoZZJjd3KrbnegdJJwfGWjc3WgSFMxVixiRUO1S{whUTh3VjygUFkxVSCvdYThcpQh\2WwZTDhcoQheG:ueX3vdpBpcXOvIHH0JGk{PiCyb4PpeIlwdiCrbn\lZ5Rm\CCrbjDoeY1idiClb4LkJIJtd2:mIH3vco9vfWOuZXHyJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25iYX\0[ZIhPDhiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJuIFXDOVA:Oy52N{ROwG0v MlP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MESxNlMoRjJyNEC0NVI{RC:jPh?=
TZM-bl MVrBcpRqfmm{YXygZZN{[Xl? M2W0PFQ5KGi{cx?= MUDBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKHO3YoT5dIUhSiCqYYLic5JqdmdicILveIVie2VicH;sfY1wenCqaYPtJIF1KE1|NjDwc5NqfGmxbjDpcoZm[3SnZDDpckBpfW2jbjDUXm0u[mxiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 NIjscHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESwOFEzOyd-MkC0NFQyOjN:L3G+
TZM-bl NU[wRpdQSW62aY\pdoFtKGG|c3H5 NV7lOGJ2PDhiaILz M3jBfWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFic4XieJlx\SCFUl[wNn9CTyCqYYLic5JqdmdicILveIVie2VicH;sfY1wenCqaYPtJIF1KEl|NjDwc5NqfGmxbjDpcoZm[3SnZDDpckBpfW2jbjDUXm0u[mxiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 M1W2bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEC0NVI{Lz5{MESwOFEzOzxxYU6=
TZM-bl NFHocHNCdnSrdnnyZYwh[XO|YYm= MlX0OFghcHK| NVvIUYdiSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDzeYJ1gXCnIFKgbIFz[m:{aX7nJJBzd3SnYYPlJJBwdHmvb4LwbIl{dSCjdDDJN|YheG:|aYTpc44hcW6oZXP0[YQhcW5iaIXtZY4hXFqPLXLsJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25iYX\0[ZIhPDhiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?= NYX4dI5MRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0NFQyOjNpPkKwOFA1OTJ|PD;hQi=>
MT4 NWXJSoJKSW62aY\pdoFtKGG|c3H5 M1jGNGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJdqdGRidInw[UBJUVZzIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKE2WNDDj[YxteyCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wOFXPxE1w MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR|OU[xNkc,OjB2M{m2NVI9N2F-
MT4 NUO4RpZJSW62aY\pdoFtKGG|c3H5 NU[5dI1wSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDlfJBz\XO|aX7nJJBzd3SnYYPlJGwyOElxTEK0TU9OPD[LL2[4NmkwUTh2VjDteZRidnRiaX7m[YN1\WRiaX6gbJVu[W5iTWS0JINmdGy|IIPlcIVkfGWmIHHmeIVzKDVyIIDhd5Ni\2W|IH;mJGdTVC1{MU[gZpkhVVSWIHHzd4F6NCCHQ{WwQVAvOTQQvF2u MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR|OU[xNkc,OjB2M{m2NVI9N2F-
MT4 NFf4OpVCdnSrdnnyZYwh[XO|YYm= MnXqNUB2VQ>? MnqzRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCneIDy[ZN{cW6pIIDyc5Rm[XOnIFyxNGYwVTR4TTzJM3E3OVFibYX0ZY51KGmwZnXjeIVlKGmwIHj1cYFvKE2WNDDj[YxteyC|ZXzlZ5Rm\CCjdDCxJJVOKG:oIFfSUE0{QTZiYomgUXRVKGG|c3H5MEBGSzVyPUCuNlLPxE1w NF22S|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESzPVYyOid-MkC0N|k3OTJ:L3G+
MT4 M3[zWGFvfGm4aYLhcEBie3OjeR?= MV[xJJVO NUPucXZMSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDlfJBz\XO|aX7nJJBzd3SnYYPlJGwyOEZxTUS2TU9VQTGVIH31eIFvfCCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNic3Xs[YN1\WRiYYSgNUB2VSCxZjDHVmwuOjR4IHL5JG1VXCCjc4PhfUwhTUN3ME2wMlI6|ryPLh?= M2fU[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO5OlEzLz5{MESzPVYyOjxxYU6=
MT4 M4nMOGFvfGm4aYLhcEBie3OjeR?= M3nOOFUhfU1? MkHiRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCneIDy[ZN{cW6pIIDyc5Rm[XOnIFyxNGYwVTR4ST;JOVBXN0F5MW[vTVg1Xi:OOUDNJI12fGGwdDDpcoZm[3SnZDDpckBpfW2jbjDNWFQh[2WubIOgd4Vt\WO2ZXSgZZQhPSC3TTDv[kBidXC{ZX7heolzKGK7IF3UWEBie3OjeTygSWM2OD1yLkROwG0v NX;kR4J[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0N|k3OTJpPkKwOFM6PjF{PD;hQi=>
MT4 M4O5UGFvfGm4aYLhcEBie3OjeR?= MYS1JJVO NX7IVWdSSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDlfJBz\XO|aX7nJJBzd3SnYYPlJGwyOEZxTUS2TU9KPTSYL2[4NmEhdXW2YX70JIlv\mWldHXkJIlvKGi3bXHuJG1VPCClZXzsd{B{\WynY4Tl[EBifCB3IIXNJI9nKEyxcHnuZZZqeiCkeTDNWHQh[XO|YYmsJGVEPTB;MD6zNu69VS5? NGqzVGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESzPVYyOid-MkC0N|k3OTJ:L3G+
MT4 NULPSFBvSW62aY\pdoFtKGG|c3H5 NXO3fpNFOSC3TR?= NYT0WFB2SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDlfJBz\XO|aX7nJJBzd3SnYYPlJGwyOEZxTUS2UE9KPTCYL1G3NXZufXSjboSgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJJNmdGWldHXkJIF1KDFidV2gc4YhT1KOLUK4OkBjgSCPVGSgZZN{[XluIFXDOVA:OC5|N988UU4> Mm\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2M{m2NVIoRjJyNEO5OlEzRC:jPh?=
MT4 M37CUGFvfGm4aYLhcEBie3OjeR?= NXz5cWs3PSC3TR?= M{i0dGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiZYjwdoV{e2mwZzDwdo91\WG|ZTDMNVBKN0d2OG[vTVU1Xi:DN{HWM2k5PFZxTEmwUUBufXSjboSgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJJNmdGWldHXkJIF1KDVidV2gc4Yhe2GzdXnuZZZqeiCkeTDNWHQh[XO|YYmsJGVEPTB;MD61Ne69VS5? M2[5ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO5OlEzLz5{MESzPVYyOjxxYU6=
BESM MYXBcpRqfHK7cHHuc5NwdWGuIHHzd4F6 MVi4PEBpenN? NGr3TmxCdnSrdIL5dIFvd3OxbXHsJIFkfGm4aYT5JIFo[Wmwc4SgWJJ6eGGwb4PvcYEh[3K3enmgZY1ie3SrZ3;0[ZMhcW6oZXP0[YQhcW5iQlXTUUBk\WyuczDt[YF{fXKnZDDh[pRmeiB6ODDodpMheG:|dHnu[oVkfGmxbjDifUBJXFNiYYPzZZktKEWFNUC9NVLPxE1w M{T3[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUS3PFE6Lz5{MEW0O|gyQTxxYU6=
BESM M{W4OmN6fG:2b4jpZ4l1gSCjc4PhfS=> MlP3PFghcHK| M3XJN2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KEKHU12gZ4VtdHNiYX\0[ZIhQDhiaILzJIJ6KEiWUzDhd5NigSxiRVO1NF0{Os7:TT6= NID0c289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEW0O|gyQSd-MkC1OFc5OTl:L3G+
CMEC/D3 MUfGeY5kfGmxbjDhd5NigQ>? NI\HRpk{OCCvaX7z M4TyTWlvcGmkaYTpc44hd2ZiUD3ndEBqdiCqdX3hckBEVUWFL1SzJINmdGy|IIXzbY5oKE6ERD3BZoEh[XNic4Xid5Rz[XSnIHHmeIVzKDNyIH3pcpMh[nliZnzve{BkgXSxbXX0dolkKGGwYXz5d4l{NCCLQ{WwQVEvP87:TT6= M331clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUC1PVI5Lz5|MUWwOVkzQDxxYU6=
CMEC/D3 NIDkXJRHfW6ldHnvckBie3OjeR?= NGfaZ2w{OCCvaX7z NUL4Oo5LUW6qaXLpeIlwdiCxZjDQMYdxKGmwIHj1cYFvKEOPRVOvSFMh[2WubIOgeZNqdmdiY3HsZ4Vqdi2DTTDhd{B{fWK|dILheIUh[W[2ZYKgN|AhdWmwczDifUBndG:5IHP5eI9u\XS{aXOgZY5idHm|aYOsJGlEPTB;Mt88UU4> M3rZV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUC1PVI5Lz5|MUWwOVkzQDxxYU6=
12D7-MDR MWrGeY5kfGmxbjDhd5NigQ>? NUnGRVlmOzBibXnudy=> NF;MfG1KdmirYnn0bY9vKG:oIGCt[5AhcW5iaIXtZY4hOTKGNz3NSHIh[2WubIOgeZNqdmdiTlLEMWFj[SCjczDzeYJ{fHKjdHWgZYZ1\XJiM{CgcYlveyCkeTDmcI94KGO7dH;t[ZRzcWNiYX7hcJl{cXNuIFnDOVA:PS56zszNMi=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTVyNUmyPEc,OzF3MEW5Nlg9N2F-
12D7-MDR MVLGeY5kfGmxbjDhd5NigQ>? NWDJdo5JOzBibXnudy=> NYjRS3VCUW6qaXLpeIlwdiCxZjDQMYdxKGmwIHj1cYFvKDF{REetUWRTKGOnbHzzJJV{cW6pIHPhcINmcW5vQV2gZZMhe3Wkc4TyZZRmKGGodHXyJFMxKG2rboOgZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nzMEBKSzVyPUmuNe69VS5? M{TyfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUC1PVI5Lz5|MUWwOVkzQDxxYU6=
12D7 MYjBcpRqfmm{YXygZZN{[Xl? NUPocJFsOyCmYYnz MVzBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKEyDSTDpcoZm[3SnZDDpckBpfW2jbjCxNmQ4KGOnbHzzJI93\XKneIDy[ZN{cW6pIICt[5Ah[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJHAzPCCuZY\lcEBifCBzOkGgdoF1cW9ib3[geIV{fCClb33wc5Vv\CC2bzDhZoFk[X[rcjDpcoN2[mG2ZXSg[o9zKDNiZHH5d{Bnd2yub4fl[EBjgSCob3zsc5dm\CCkeTDy[ZBt[WOnbXXueEBw\iCocnXzbEBu\WSrdX2gZ49vfGGrbnnu[{Bkd22yb4Xu[C=> NWT5Spc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1NFU6OjhpPkOxOVA2QTJ6PD;hQi=>
12D7 MnyxRY51cX[rcnHsJIF{e2G7 MWGwMlA5KHSxIEGuNlUhfU1? Ml7jN{Bl[Xm| M4q5SmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiTFHJJIlv\mWldHXkJIlvKGi3bXHuJFEzTDdiY3XscJMhd3[ncnX4dJJme3OrbnegdE1oeCCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hWDJ2IHzleoVtKGG2IECuNFghfG9iMT6yOUB2VSCrbnP1ZoF1\WRiZn;yJFMh\GG7czDmc4xtd3enZDDifUBz\XCuYXPlcYVvfCCxZjDmdoV{cCCvZXTpeY0h[2:wdHHpcolv\yClb33wc5Vv\CCjbnSgcYVie3W{ZXSgZYZ1\XJiMzDkZZl{KGK7IFXMTXNC MkXhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3MEW5NlgoRjNzNUC1PVI5RC:jPh?=
SJ-GBM2 Moi4dWhVWyCjc4PhfS=> NGmyfYxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1qtS2JOOiClZXzsdy=> NHzncZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC NWLkZnNoeUiWUzDhd5NigQ>? NWjVS3dNeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
U-2 OS NGW0NJZyUFSVIHHzd4F6 M1rleZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCXLUKgU3Mh[2WubIO= MkDOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Saos-2 M33yXpFJXFNiYYPzZZk> NEHMVYZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU3Hvd{0zKGOnbHzz MnjqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
OHS-50 MlT2dWhVWyCjc4PhfS=> M3i0dpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIO= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD MlzidWhVWyCjc4PhfS=> Ml7jdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ M2fUWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) NHr5[3ZyUFSVIHHzd4F6 MlP4dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy| NEPHUWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MT2 NGPJUI1CdnSrdnnyZYwh[XO|YYm= MYW1JIRigXN? NHzuUZlCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEB4cWymIIT5dIUhUEmYMTCzRkBqdm[nY4Tl[EBqdiCqdX3hckBOXDJiY3XscJMh[XO|ZYPz[YQh[XNidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBi\nSncjC1JIRigXNiYomgXHRVKGG|c3H5MEBGSzVyPUCuNFA1Qc7:TT6= NF\GPW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2QDRxJ{7DbGVOSkx:L3G+
HepG2 M4P1OWN6fG:2b4jpZ4l1gSCjc4PhfS=> NUnZ[XdKPSCmYYnz NYLle4RRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IEWg[IF6eyCkeTDYWHQh[XO|YYmsJGNEPTB;Nz60{txONg>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1PFQwLz6FaFXNRmw9N2F-
skeletal myoblast cells M4PLfGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGnFT3U4OiCqb4Xydy=> NYiyeIpoTE6GSUqgR5l1d3SxeHnjbZR6KGmwIG\peJJwNCB5MjDoc5VzNCCrbjDyZZQhe2unbHX0ZYwhdXmxYnzhd5Qh[2WubIOsJGlEPTB;MUKuNFXPxE1w MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1PFQwLz6FaFXNRmw9N2F-
MT2 M4LGWGN6fG:2b4jpZ4l1gSCjc4PhfS=> NYTjXpBNPSCmYYnz MnS4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXQzKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA2KGSjeYOgZpkhYFSWIHHzd4F6NCCFQ{WwQVE6NjIQvF2u M{jEflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU5PC9pPlPoSW1DVDxxYU6=
Assay
Methods Test Index PMID
Growth inhibition assay Cell viability 22271897
Western blot p-eIF2Aα / eIF2Aα / ATF4 / GRP78 / CHOP ; SOD1 / SOD2 / catalase ; PAX3 / MITF 21697087 27280849 26977879
Immunofluorescence LC3 / p62 ; PAX3 / MITF ; p-SMAD2 27330277 26977879
In vivo In vivo studies indicate that AG1343 is well absorbed orally in a variety of species and possesses favorable pharmacokinetic properties in humans. Initial investigation of the compound in fed rats indicated an oral bioavailability of 43%. In contrast, the oral bioavailability was significantly reduced in fasted rats to 29%. Nelfinavir demonstrated significant oral bioavailabilability across a range of species including dogs (47%), marmosets (17%), and cynomolgus monkeys (26%). It has a long plasma half-life after oral dosing that is likely due to a combination of slow dissolution and absorption. nelfinavir is well absorbed in humans particularly when administered with food and possesses minimal and easily managed side effects[1].

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: Sprague-Dawley rats
  • Dosages: 12.5 to 25 mg/kg (i.v); 25 to 50 mg/kg (oral)
  • Administration: i.v and oral administration

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(150.62 mM)
Water Insoluble
Ethanol ''100 mg/mL

Chemical Information

Molecular Weight 663.89
Formula

C33H49N3O7S2

CAS No. 159989-65-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C=CC=C1O)C(=O)NC(CSC2=CC=CC=C2)C(CN3CC4CCCCC4CC3C(=O)NC(C)(C)C)O.CS(=O)(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Nelfinavir (AG 1343) Mesylate | Nelfinavir (AG 1343) Mesylate supplier | purchase Nelfinavir (AG 1343) Mesylate | Nelfinavir (AG 1343) Mesylate cost | Nelfinavir (AG 1343) Mesylate manufacturer | order Nelfinavir (AG 1343) Mesylate | Nelfinavir (AG 1343) Mesylate distributor