Nelfinavir (AG 1343) Mesylate

Catalog No.S4282 Synonyms: Viracept

For research use only.

Nelfinavir Mesylate (Viracept, AG1343) is a potent HIV protease inhibitor with Ki of 2 nM.

Nelfinavir (AG 1343) Mesylate Chemical Structure

CAS No. 159989-65-8

Selleck's Nelfinavir (AG 1343) Mesylate has been cited by 16 publications

Purity & Quality Control

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Biological Activity

Description Nelfinavir Mesylate (Viracept, AG1343) is a potent HIV protease inhibitor with Ki of 2 nM.
Targets
HIV protease [1]
(Cell-free assay)
2 nM(Ki)
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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OHS-50 NUXEVXdNeUiWUzDhd5NigQ>? NILtb4hyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzz M1jiUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD Mn3rdWhVWyCjc4PhfS=> NXXkOG1ReUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLEJINmdGy| NEP3bnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) NEnUeopyUFSVIHHzd4F6 MYjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? NFnCXHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MT2 M2DWW2FvfGm4aYLhcEBie3OjeR?= NEfB[nU2KGSjeYO= MknBRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sge4lt\CC2eYDlJGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gUXQzKGOnbHzzJIF{e2W|c3XkJIF{KH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZYZ1\XJiNTDkZZl{KGK7IGjUWEBie3OjeTygSWM2OD1yLkCwOFnPxE1w MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1PFQwLz6FaFXNRmw9N2F-
HepG2 NGPEXGVEgXSxdH;4bYNqfHliYYPzZZk> NIW5boQ2KGSjeYO= M1\TUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB3IHThfZMh[nliWGTUJIF{e2G7LDDDR|UxRTdwNN88UU4> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1PFQwLz6FaFXNRmw9N2F-
skeletal myoblast cells M3nmNWN6fG:2b4jpZ4l1gSCjc4PhfS=> MXW3NkBpd3W{cx?= M372[GRPTEl8IFP5eI91d3irY3n0fUBqdiCYaYTyc{whPzJiaH;1dkwhcW5icnH0JJNs\WyndHHsJI16d2KuYYP0JINmdGy|LDDJR|UxRTF{LkC1{txONg>? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1PFQwLz6FaFXNRmw9N2F-
MT2 M{XnXmN6fG:2b4jpZ4l1gSCjc4PhfS=> MmGwOUBl[Xm| NGDYVWxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXDJiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDViZHH5d{BjgSC[VGSgZZN{[XluIFPDOVA:OTlwMd88UU4> NEjEXnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2QDRxJ{7DbGVOSkx:L3G+
Assay
Methods Test Index PMID
Growth inhibition assay Cell viability 22271897
Western blot p-eIF2Aα / eIF2Aα / ATF4 / GRP78 / CHOP ; SOD1 / SOD2 / catalase ; PAX3 / MITF 21697087 27280849 26977879
Immunofluorescence LC3 / p62 ; PAX3 / MITF ; p-SMAD2 27330277 26977879
In vivo In vivo studies indicate that AG1343 is well absorbed orally in a variety of species and possesses favorable pharmacokinetic properties in humans. Initial investigation of the compound in fed rats indicated an oral bioavailability of 43%. In contrast, the oral bioavailability was significantly reduced in fasted rats to 29%. Nelfinavir demonstrated significant oral bioavailabilability across a range of species including dogs (47%), marmosets (17%), and cynomolgus monkeys (26%). It has a long plasma half-life after oral dosing that is likely due to a combination of slow dissolution and absorption. nelfinavir is well absorbed in humans particularly when administered with food and possesses minimal and easily managed side effects[1].

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: Sprague-Dawley rats
  • Dosages: 12.5 to 25 mg/kg (i.v); 25 to 50 mg/kg (oral)
  • Administration: i.v and oral administration

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 663.89
Formula

C33H49N3O7S2

CAS No. 159989-65-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C=CC=C1O)C(=O)NC(CSC2=CC=CC=C2)C(CN3CC4CCCCC4CC3C(=O)NC(C)(C)C)O.CS(=O)(=O)O

In vivo Formulation Calculator (Clear solution)

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%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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