Kobe2602

Catalog No.S6527

For research use only.

Kobe2602 is a selective Ras inhibitor that blocks H-Ras GTP binding to c-Raf-1 (Ki = 149 µM).

Kobe2602 Chemical Structure

CAS No. 454453-49-7

Purity & Quality Control

Choose Selective Ras Inhibitors

Biological Activity

Description Kobe2602 is a selective Ras inhibitor that blocks H-Ras GTP binding to c-Raf-1 (Ki = 149 µM).
Targets
Ras [1]
()
149 μM(Ki)

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: NIH 3T3 cells
  • Concentrations: 20 μM
  • Incubation Time: 1 h
  • Method:

    NIH 3T3 cells are transfected with pEF-BOS-HA-K-Ras(G12V) or an empty vector and treated with 20 μM Kobe0065, 20 μM Kobe2602, or the vehicle (DMSO) in the presence of 2% (vol/vol) FBS for 1 h. Cell lysate is subjected to detection of c-Raf-1 coimmunoprecipitated with K-Ras(G12V) by an anti-HA antibody and total c-Raf-1 by Western blotting with an anti-c-Raf-1 antibody.

Animal Research:

[1]

  • Animal Models: Female athymic nude mice implanted with SW480 cells
  • Dosages: 80 mg/kg
  • Administration: oral

Solubility (25°C)

In vitro

DMSO 84 mg/mL
(200.32 mM)
Ethanol 40 mg/mL
(95.39 mM)
Water Insoluble

Chemical Information

Molecular Weight 419.31
Formula

C14H9F4N5O4S

CAS No. 454453-49-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1NC(=S)NNC2=C(C=C(C=C2[N+](=O)[O-])C(F)(F)F)[N+](=O)[O-])F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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