ISO-1

For research use only.

Catalog No.S7732 Synonyms: MIF Antagonist

6 publications

ISO-1 Chemical Structure

CAS No. 478336-92-4

ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.

Selleck's ISO-1 has been cited by 6 publications

Purity & Quality Control

Choose Selective MIF Inhibitors

Biological Activity

Description ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.
Targets
MIF [1]
()
7 μM
In vitro

ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages[2].

In vivo

ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP)[2]. It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. ISO-1 attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema[3]. ISO-1 protects photoreceptors and reduces gliosis in experimental retinal detachment[4].

Protocol

Cell Research:

[2]

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  • Cell lines: RAW 267.4 macrophages
  • Concentrations: 1-100 μM
  • Incubation Time: 30 min
  • Method:

    RAW 267.4 macrophages were treated with various concentrations of ISO-1 (1-100 μM) 30 min prior to LPS addition. Macrophages were collected 2 h after activation with LPS and washed one time in PBS (pH 7.4). Nuclear extract was isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: Male Balb/C mice, ∼8 weeks old, subjectd to endotoxemia or cecal ligation and puncture (CLP)
  • Dosages: 3.5-35 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 47 mg/mL (199.79 mM)
Water Insoluble
Ethanol '47 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 235.24
Formula

C12H13NO4

CAS No. 478336-92-4
Storage powder
in solvent
Synonyms MIF Antagonist
Smiles COC(=O)CC1CC(=NO1)C2=CC=C(C=C2)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02284919 Active not recruiting Drug: [18F]ISO-1 Breast Cancer|Breast Neoplasm University of Pennsylvania November 2014 Phase 1
NCT02204202 Terminated Drug: [18F]FDG|Drug: [18F]ISO-1 Lung Disease Washington University School of Medicine|National Heart Lung and Blood Institute (NHLBI) February 2014 --

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID