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ISO-1 MIF inhibitor

Name: ISO-1
Brand: Selleck Chemicals
SKU: S7732
Availability: InStock
Rating: 5 (18 reviews)

Cat.No.S7732

ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.

Chemical Structure

Molecular Weight: 235.24

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.89%

99.89

Related Targets	Immunology & Inflammation related CD markers Interleukins Anti-infection Antioxidant COX Histamine Receptor TNF-alpha ROS Parasite PD-1/PD-L1 TLR Prostaglandin Receptor IFN CXCR Nrf2 NOS CCR NLRP3 eIF STING AhR IL Receptor Complement System IRAK β-lactamase IDO/TDO PGES NADPH-oxidase FKBP
Related Products	4-IPP

Chemical Information, Storage & Stability

Molecular Weight	235.24	Formula	C₁₂H₁₃NO₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	478336-92-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	MIF Antagonist			Smiles	COC(=O)CC1CC(=NO1)C2=CC=C(C=C2)O

Solubility

In vitro
Batch:

DMSO : 47 mg/mL ( (199.79 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 47 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (21.25mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	3% DMSO 1 40% PEG 300 2 5%Tween80 3 52%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.500mg/ml (19.13mM)	Taking the 1 mL working solution as an example, add 30 μL of 150 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 520 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	MIF ^[1] 7 μM
In vitro	ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages^[2].
In vivo	ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP)^[2]. It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. This compound attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema^[3]. It protects photoreceptors and reduces gliosis in experimental retinal detachment^[4].
References	https://pubmed.ncbi.nlm.nih.gov/19090668/ https://pubmed.ncbi.nlm.nih.gov/16115897/ https://pubmed.ncbi.nlm.nih.gov/26752192/ https://www.nature.com/articles/s41598-017-14298-9

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT02284919	Active not recruiting	Breast Cancer\|Breast Neoplasm	University of Pennsylvania	November 2014	Phase 1
NCT02204202	Terminated	Lung Disease	Washington University School of Medicine\|National Heart Lung and Blood Institute (NHLBI)	February 2014	--

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