ISO-1

Catalog No.S7732 Synonyms: MIF Antagonist

For research use only.

ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.

ISO-1 Chemical Structure

CAS No. 478336-92-4

Selleck's ISO-1 has been cited by 8 Publications

Purity & Quality Control

Choose Selective MIF Inhibitors

Biological Activity

Description ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.
Targets
MIF [1]
()
7 μM
In vitro

ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages[2].

In vivo

ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP)[2]. It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. ISO-1 attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema[3]. ISO-1 protects photoreceptors and reduces gliosis in experimental retinal detachment[4].

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: RAW 267.4 macrophages
  • Concentrations: 1-100 μM
  • Incubation Time: 30 min
  • Method:

    RAW 267.4 macrophages were treated with various concentrations of ISO-1 (1-100 μM) 30 min prior to LPS addition. Macrophages were collected 2 h after activation with LPS and washed one time in PBS (pH 7.4). Nuclear extract was isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents.

Animal Research:

[2]

  • Animal Models: Male Balb/C mice, ∼8 weeks old, subjectd to endotoxemia or cecal ligation and puncture (CLP)
  • Dosages: 3.5-35 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

DMSO 47 mg/mL
(199.79 mM)
Water Insoluble
Ethanol '47 mg/mL

Chemical Information

Molecular Weight 235.24
Formula

C12H13NO4

CAS No. 478336-92-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC(=O)CC1CC(=NO1)C2=CC=C(C=C2)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02284919 Active not recruiting Drug: [18F]ISO-1 Breast Cancer|Breast Neoplasm University of Pennsylvania November 2014 Phase 1
NCT02204202 Terminated Drug: [18F]FDG|Drug: [18F]ISO-1 Lung Disease Washington University School of Medicine|National Heart Lung and Blood Institute (NHLBI) February 2014 --

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy ISO-1 | ISO-1 supplier | purchase ISO-1 | ISO-1 cost | ISO-1 manufacturer | order ISO-1 | ISO-1 distributor