ISO-1

Catalog No.S7732

ISO-1 Chemical Structure

Molecular Weight(MW): 235.24

ISO-1 is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.

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Biological Activity

Description ISO-1 is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.
Targets
MIF [1]
()
7 μM
In vitro

ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages[2].

In vivo

ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP)[2]. It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. ISO-1 attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema[3]. ISO-1 protects photoreceptors and reduces gliosis in experimental retinal detachment[4].

Protocol

Cell Research:

[2]

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  • Cell lines: RAW 267.4 macrophages
  • Concentrations: 1-100 μM
  • Incubation Time: 30 min
  • Method:

    RAW 267.4 macrophages were treated with various concentrations of ISO-1 (1-100 μM) 30 min prior to LPS addition. Macrophages were collected 2 h after activation with LPS and washed one time in PBS (pH 7.4). Nuclear extract was isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: Male Balb/C mice, ∼8 weeks old, subjectd to endotoxemia or cecal ligation and puncture (CLP)
  • Formulation: aqueous 5% dimethyl sulfoxide
  • Dosages: 3.5-35 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 47 mg/mL (199.79 mM)
Ethanol 47 mg/mL (199.79 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 235.24
Formula

C12H13NO4

CAS No. 478336-92-4
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03057743 Recruiting METASTATIC BREAST CANCER Abramson Cancer Center of the University of Pennsylvania April 5 2016 --
NCT03057743 Recruiting METASTATIC BREAST CANCER Abramson Cancer Center of the University of Pennsylvania April 5 2016 --
NCT02284919 Active not recruiting Breast Cancer|Breast Neoplasm University of Pennsylvania November 2014 Phase 1
NCT02284919 Active not recruiting Breast Cancer|Breast Neoplasm University of Pennsylvania November 2014 Phase 1
NCT02204202 Recruiting Lung Disease Washington University School of Medicine|National Heart Lung and Blood Institute (NHLBI) February 2014 --
NCT02204202 Recruiting Lung Disease Washington University School of Medicine|National Heart Lung and Blood Institute (NHLBI) February 2014 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID