ISO-1

Catalog No.S7732 Batch:S773204

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Technical Data

Formula

C12H13NO4
Molecular Weight 235.24 CAS No. 478336-92-4
Solubility (25°C)* In vitro DMSO 47 mg/mL (199.79 mM)
Ethanol 47 mg/mL (199.79 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
3% DMSO 40% PEG 300 5%Tween80 52%ddH2O
4.5mg/ml Taking the 1 mL working solution as an example, add 30 μL of 150 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 520 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description ISO-1 (MIF Antagonist) is an inhibitor of MIF (Macrophage migration inhibitory factor) D-dopachrome tautomerase activity with an IC50 of ~7 μM.
Targets
MIF [1]
7 μM
In vitro

ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages[2].
In vivo

ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP)[2]. It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. ISO-1 attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema[3]. ISO-1 protects photoreceptors and reduces gliosis in experimental retinal detachment[4].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    RAW 267.4 macrophages

  • Concentrations

    1-100 μM

  • Incubation Time

    30 min

  • Method

    RAW 267.4 macrophages were treated with various concentrations of ISO-1 (1-100 μM) 30 min prior to LPS addition. Macrophages were collected 2 h after activation with LPS and washed one time in PBS (pH 7.4). Nuclear extract was isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents.
Animal Study:

[2]
  • Animal Models

    Male Balb/C mice, ∼8 weeks old, subjectd to endotoxemia or cecal ligation and puncture (CLP)

  • Dosages

    3.5-35 mg/kg

  • Administration

    i.p.

Selleck's ISO-1 has been cited by 14 publications

MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinoma [ iScience, 2023, 26(8):107273] PubMed: 37520719
MIF Increases sFLT1 Expression in Early Uncomplicated Pregnancy and Preeclampsia [ Int J Mol Sci, 2023, 24(12)10050] PubMed: 37373198
Therapeutic targeting of tumor spheroids in a 3D microphysiological renal cell carcinoma-on-a-chip system [ Neoplasia, 2023, 10.1016/j.neo.2023.100948] PubMed: 37944353
Ischemic stroke protected by ISO-1 inhibition of apoptosis via mitochondrial pathway [ Sci Rep, 2023, 13(1):2788] PubMed: 36797398
On the origin of metastases: Induction of pro-metastatic states after impending cell death via ER stress, reprogramming, and a cytokine storm [ Cell Rep, 2022, 38(10):110490] PubMed: 35263600
Exosomes from adipose-derived stem cells regulate M1/M2 macrophage phenotypic polarization to promote bone healing via miR-451a/MIF [ Stem Cell Res Ther, 2022, 13(1):149] PubMed: 35395782
Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI [ Cell Death Discov, 2022, 8(1):61] PubMed: 35165294
MIF/CXCR4 signaling axis contributes to survival, invasion, and drug resistance of metastatic neuroblastoma cells in the bone marrow microenvironment [ BMC Cancer, 2022, 22(1):669] PubMed: 35715791
Isopsoralen ameliorates rheumatoid arthritis by targeting MIF [ Arthritis Res Ther, 2021, 23(1):243] PubMed: 34535196
Utx Regulates the NF-κB Signaling Pathway of Natural Stem Cells to Modulate Macrophage Migration during Spinal Cord Injury [ J Neurotrauma, 2021, 38(3):353-364] PubMed: 32977735

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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