Ganciclovir

Catalog No.S1878 Synonyms: RS-21592, BW-759

Ganciclovir Chemical Structure

Molecular Weight(MW): 255.23

Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.

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In DMSO USD 90 In stock
USD 70 In stock
USD 270 In stock
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Cited by 3 Publications

1 Customer Review

  • Effect of ganciclovir on viral DNA accumulation (A,B) and induction of TGF-β1 production after human cytomegalovirus infection in human trabecular meshwork cells (C). Cells were harvested at 5 day post-infection at a high multiplicity of infection (MOI 1) under the treatment with different concentrations of ganciclovir (GAN). Treatment with 10 μmol of ganciclovir significantly decreased the viral DNA accumulation (p < 0.001) (A,B). However, treatment with ganciclovir did not affect the TGF-β production using a TGF-β1 luciferase bioassay. Results are expressed as the mean +/− standard deviation of three different experiments.

    Sci Rep, 2017, 7: 43349. Ganciclovir purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.
In vitro

Ganciclovir is metabolized to the triphosphate form by primarily three cellular enzymes: (1) a deoxyguanosine kinase induced by CMV-infected cells; (2) guanylate kinase; and (3) phosphoglycerate kinase. [1] Ganciclovir is sufficient to induce cell death in most bystander cells cocultured with HSV-tk-expressing cells. [2] Ganciclovir significantly reduces DNA synthesis in the transformed cells, whereas Ganciclovir has little effect on DNA synthesis in the nontransformed cells. Ganciclovir exhibits a concentration-dependent reduction in the rate of elongation into mature DNA. [3] Ganciclovir induces cell cycle arrests rather than direct chemical effect on HSVtk-transduced B16F10 melanoma cells. [4] Ganciclovir produces only weak inhibition of DNA synthesis. Ganciclovir-treated cells accumulate in early S-phase and remained there until cell death, suggesting that ganciclovir incorporation in the DNA template is important for cytotoxicity. [5] Ganciclovir-induced apoptosis is due to incorporation of the drug into DNA resulting in replication-dependent formation of DNA double-strand breaks and, at later stages, S and G2/M arrest. Ganciclovir-provoked DNA instability is likely to be responsible for the observed initial decline in Bcl-2 level and caspase-9/-3 activation. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
E6SM cell lines M1LsemZ2dmO2aX;uJIF{e2G7 M3PZfGVn\mWldHn2[UBkd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgTIVzeGW|IIPpcZBt\XhidnnyeZMuOiBqSGPWMVIqKGmwZIXj[YQh[3m2b4DheIhq[2m2eTDifUA2OCViaX6gSVZUVSClZXzsJIxqdmW|LDDFR|UxRTFwMjDuUS=> NITYboIyOTR7NUW4Oi=>
human OST TK-cells MVvDfZRwfG:6aXRCpIF{e2G7 NXHXfGI1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hV1OWIGTLMYNmdGy|IHX4dJJme3OrbnegTHNXOSCWSzygTWM2OD1zLkmgcm0> NXPGWnlFOTdzOEGxOVg>
HEL cells MoDjSpVv[3Srb36gZZN{[Xl? MXnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIV3YyKEuRUzDpcoZm[3SnZDDpckBJTUxiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXK3cz3pcoR2[2WmIHP5eI9x[XSqaXOg[YZn\WO2LDDFR|UxRTFyIH7N NEfqdFYzOTJ|MkiyPC=>
HFF cells NFPNZolHfW6ldHnvckBie3OjeR?= NH;WUWcyOCCmYYnz NUP6RVZwSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOPVjDUc5dv\SCrbn\lZ5Rm\CCrbjDISmYh[2WubIOgbY5kfWKjdHXkJIZweiBzMDDkZZl{KGK7IIDsZZF2\SC{ZXT1Z5Rqd25iYYPzZZktKEmFNUC9NE4yPCEQvF2= M1nMZlIyQDF{NEKw
MCA-TK cells MVzDfZRwfG:6aXRCpIF{e2G7 M2jNZ2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2FQT3UT{Bk\WyuczygTWM2OD1yLkG1JO69VQ>? MkPVNVc6PjB7Mk[=
HFF cells M{KyNmZ2dmO2aX;uJIF{e2G7 MlfRTY5pcWKrdHnvckBw\iCKQ13WJGFFOTZ7IILldIxq[2G2aX;uJIlvKEiIRjDj[YxteyCkeTDjfZRweGG2aHnjJIVn\mWldDDhd5NigSxiRVO1NF0xNjF3IN88US=> NHLyfW0yPzByNEeyOi=>
Vero cells M2\ZZmZ2dmO2aX;uJIF{e2G7 NUXMSlU4SW62aY\pdoFtKGGldHn2bZR6KGSndHXycYlv\WRiYXfhbY5{fCCqZYLw[ZMhe2mvcHzlfEB1gXCnIEGgLGYhe3S{YXnuLUBjgSCybHHxeYUhemWmdXP0bY9vKGmwIG\ldo8h[2WubIOsJGlFPTB;MD6yJO69VQ>? M3rZdlMxOTZ{NkO=
human HS27 cells Mn7QSpVv[3Srb36gZZN{[Xl? NE\rNGM4KGSjeYO= NXLQRZk1SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gZ5l1d22nZ3Hsc5ZqenW|IHnu[oVkfGWmIHnuJIh2dWGwIFjTNlch[2WubIOgZYZ1\XJiNzDkZZl{KGK7IFfGVE1j[XOnZDDmcJVwemW|Y3XueEBz\WS3Y4Tpc44h[XO|YYmsJGVEPTB;MD6zNkDPxE1? NXLUdlFiOjByNEe5NVE>
MRC5 cells NHHEVIFHfW6ldHnvckBie3OjeR?= MmHsRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGNOXiCrbjDNVmM2KGOnbHzzJIJ6KHCuYYH1[UBz\WS3Y4Tpc44h[XO|YYmsJGlEPTB;MD65NUDPxE1? NHfvW20yPzJ|OUW5OC=>
BSC-1 cells MWHGeY5kfGmxbjDhd5NigQ>? M33tN2FvfGm4aYLhcEBi[3Srdnn0fUBw\iC2aHWgZ49ueG:3bnSge4F{KGW4YXz1ZZRm\CCjZ3HpcpN1KHSqZTDI[ZJx\XNic3ntdIxmgCC4aYL1d{B1gXCnLUGgbY4hSlOFLUGgZ4VtdHNuIFnDOVA:OyEQvF2= NVf5eFZqOjlzM{OwNC=>
MEF cells M1LIPGZ2dmO2aX;uJIF{e2G7 NF\3Vm9KdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDteZJqdmViY4n0c41m\2Gub4\pdpV{KHKncHzpZ4F1cW:wIHnuJG1GTiClZXzsd{B4[XNiZHX0[ZJucW6nZDDifUBxdGGzdXWgdoVlfWO2aX;uJIF{e2G7LDDJR|UxRTNwNDFOwG0> MkLVPVQ{QDBzNx?=
CEM cells M2Ttc2N6fG:2b4jpZ:Kh[XO|YYm= NFXQOGlEgXSxdH;4bYNqfHliYXfhbY5{fCCFRV2gZ4VtdHNuIFPDOVA:PSEQvF2= NI\1VVEyPTZzNUW0OS=>
mouse NIH 3T3 cells MmHXSpVv[3Srb36gZZN{[Xl? Mli0OE02KGSjeYO= NFr2fXZCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBOfXKrbnWgZ5l1d22nZ3Hsc5ZqenW|IIP0doFqdiCVbXn0bEBqdm[nY4Tl[EBqdiCvb4Xz[UBPUUhiM2SzJINmdGy|IHHmeIVzKDRidH:gOUBl[Xm|IHL5JJBt[XG3ZTDy[YR2[3Srb36gZZN{[XluIFXDOVA:PS55IN88US=> MmfrNVg1PThzMkS=
RG2TK+ cells Ml3ER5l1d3SxeHnjxsBie3OjeR?= NVfBUI5GPzJiaB?= MlzqR5l1d3SxeHnjbZR6KGGpYXnud5QhUFOYMT30b{Bo\W6nIH;2[ZJmgHC{ZYPzbY5oKFKJMmTLL{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDDR|UxRTVwOE[g{txO NU\JXIRbOTh6MEC3OlQ>
human bone marrow cells NEHLT4hEgXSxdH;4bYPDqGG|c3H5 M1LyR|E2KGSjeYO= M4jlVWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIJwdmVibXHydo94KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gR2ZWNUePIH\vdo1ifGmxbjDh[pRmeiBzNTDkZZl{NCCFQ{WwQVMxKM7:TR?= Mln3NVc{OjlzMEO=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Growth inhibition assay
Cell viability; 

PubMed: 29845211     


(A) HSV-TK-positive (HSV-TK: pLenO-GTP-HSV-TK) or negative (CON: pLenO-GTP) HXO-RB44 cells were treated with GCV at 0, 10, 20 or 40 µg/ml respectively. After 24 h post-transfection, cell viability was measured by MTT assay. (B) HSV-TK-positive (HSV-TK: pLenO-GTP-HSV-TK) or negative (CON: pLenO-GTP) HXO-RB44 cells were treated with GCV at 0, 10, 20 or 40 µg/ml, respectively for 48 h. Cell viability was also measured by MTT assay.

29845211
In vivo Antiviral drug ganciclovir (GCV) inhibits the proliferation of microglia in experimental autoimmune encephalomyelitis (EAE). GCV attenuates neuroinflammation and does not significantly restrain the peripheral immune response[7].

Protocol

Cell Research:

[7]

- Collapse
  • Cell lines: BV-2 cells 
  • Concentrations: --
  • Incubation Time: 24 h
  • Method:

    To assess cell proliferation by thymidine incorporation, a total of 2 × 10<sup>4</sup> BV-2 cells were seeded in a 96-well plate for a maximum of 12 h in 10% FBS containing DMEM and then serum starved for an additional 12 h before the addition of varying concentrations of GCV for a total of 24 h. Cultures were pulsed for the final 8 h with 1 µCi/well [3H]thymidine before incorporated radioactivity was measured using a β plate scintillation counter.


    (Only for Reference)
Animal Research:

[7]

- Collapse
  • Animal Models: C57BL/6 mice
  • Formulation: PBS
  • Dosages: 100 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 27 mg/mL warmed (105.78 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 255.23
Formula

C9H13N5O4

CAS No. 82410-32-0
Storage powder
in solvent
Synonyms RS-21592, BW-759

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03698435 Recruiting Drug: Ganciclovir|Drug: Valganciclovir Cytomegalovirus Infections University Medical Center Groningen May 25 2018 --
NCT02606266 Recruiting Drug: Valganciclovir Congenital Cytomegalovirus (CMV) Assistance Publique - Hôpitaux de Paris July 11 2017 Phase 2|Phase 3
NCT03088553 Recruiting Drug: Therapeutic Drug Monitoring Solid Organ Transplantation Rennes University Hospital February 22 2017 --
NCT02943057 Recruiting Drug: 2% guttae ganciclovir Cytomegalovirus Infections Singapore National Eye Centre October 2016 Phase 4
NCT02152358 Active not recruiting Drug: Aciclovir|Drug: Ganciclovir|Drug: Placebo Viral Pneumonia Assistance Publique Hopitaux De Marseille March 2014 Phase 4
NCT01602614 Completed -- Cytomegalovirus Infections University of Alabama at Birmingham April 2013 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID