G150

Catalog No.S8944

For research use only.

G150 is a potent and highly selective human cyclic GMP-AMP synthase (cGAS) inhibitor with IC50 of 10.2 nM.

G150 Chemical Structure

CAS No. 2369751-30-2

Selleck's G150 has been cited by 1 Publication

Purity & Quality Control

Choose Selective cGAS Inhibitors

Biological Activity

Description G150 is a potent and highly selective human cyclic GMP-AMP synthase (cGAS) inhibitor with IC50 of 10.2 nM.
Targets
h-cGAS [1]
(Cell-free assay)
10.2 nM
In vitro

The most potent h-cGAS-specific derivative with 2-amino pyridine (G150) shows inhibitory activity in THP1 cells and primary human macrophages while a complete absence of off-target effect on a diverse range of sensors. G150 also shows no substantial cellular toxicity[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: THP1 cells, primary macrophage cells
  • Concentrations: 10 μM and 5 μM
  • Incubation Time: 1 h
  • Method:

    Cellular activation of cGAS enzyme leads to IFNB1 mRNA expression in THP1, and primary human macrophage cells and is quantified using qRT-PCR. Total RNA is isolated from 5 × 105 THP1 cells per well of a 12-well plate, which are pre-incubated with G150 for 1 h. Human primary macrophages are used at 3×105 cells per well of a 12-well plate.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 391.25
Formula

C18H16Cl2N4O2

CAS No. 2369751-30-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CN(CC2=C1NC3=C2C(=CC(=C3Cl)Cl)C4=CN=C(C=C4)N)C(=O)CO

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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