RU.521

Catalog No.S6841 Synonyms: RU320521

For research use only.

RU.521 (RU320521) is an active, potent and selective inhibitor of cyclic GMP-AMP synthase (cGAS) that inhibits the cGAS-mediated signaling with IC50 of 700 nM. RU.521 binds to the cGAS/dsDNA complex with Kd of 36.2 nM.

RU.521 Chemical Structure

CAS No. 2262452-06-0

Selleck's RU.521 has been cited by 2 Publications

Purity & Quality Control

Choose Selective cGAS Inhibitors

Biological Activity

Description RU.521 (RU320521) is an active, potent and selective inhibitor of cyclic GMP-AMP synthase (cGAS) that inhibits the cGAS-mediated signaling with IC50 of 700 nM. RU.521 binds to the cGAS/dsDNA complex with Kd of 36.2 nM.
Targets
cGAS/dsDNA complex [1]
(Cell-free assay)
cGAS [1]
(Cell-free assay)
36.2 nM(Kd) 700 nM
In vitro

RU.521, a cGAS inhibitor, impares the overexpression of interferon-stimulated genes (ISGs) in response to cytolethal distending toxin (CDT), confirming the crucial role of cGAS-STING axis in CDT-induced type I IFN signaling.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: Mouse Embryonic Fibroblasts (MEFs)
  • Concentrations: 1 μM
  • Incubation Time: 3, 7, 40 days
  • Method:

    MEFs are exposed with or without STINGi or cGASi to CDT 7.5 ng/ml for the indicated times or to the H153A CDT mutant for 40 days. The mRNA level of the indicated genes is analyzed by RT-qPCR and the IL-6 or TNFα production in the culture supernatant are determined by ELISA. For ELISA assays, a 2 days exposure to LPS 100 ng/ml is used as positive control.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 415.23
Formula

C19H12Cl2N4O3

CAS No. 2262452-06-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C(=O)N(N1)C2=NC3=C(N2)C=CC(=C3Cl)Cl)C4C5=CC=CC=C5C(=O)O4

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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