Home Ubiquitin E1 Activating inhibitor DKM 2-93

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DKM 2-93 E1 Activating inhibitor

Cat.No.S5841

DKM 2-93 is a relatively selective lead inhibitor of ubiquitin-like modifier activating enzyme 5 (UBA5).
DKM 2-93 E1 Activating inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 243.69

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S584101 DMSO]49 mg/mL]false]Ethanol]14 mg/mL]false]Water]Insoluble]false Purity: 99.49%
99.49

Chemical Information, Storage & Stability

Molecular Weight 243.69 Formula

C11H14ClNO3

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 65836-72-8 -- Storage of Stock Solutions

Synonyms N/A Smiles COC1=C(C=C(C=C1)CNC(=O)CCl)OC

Solubility

In vitro
Batch:

DMSO : 49 mg/mL (201.07 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 14 mg/mL

Water : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC50/Ki
UBA5 [1]
In vitro
DKM 2-93 not only impairs pancreatic cancer cell survival in PaCa2, but also Panc1 cells with 50 % effective concentration values of 90 and 30 μM, respectively[1].
In vivo
DKM 2-93 daily treatment significantly impairs tumor growth of PaCa2 cells in vivo in tumor xenograft studies in immune-deficient mice without causing any weight loss or overt toxicity[1].
References

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