Home Microbiology SARS-CoV inhibitor Bemnifosbuvir Hemisulfate (AT-527)

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Bemnifosbuvir Hemisulfate (AT-527) SARS-CoV inhibitor

Cat.No.E1013

Bemnifosbuvir Hemisulfate (AT-527, RG-6422, RO 7496998) is a hemi-sulfate salt of AT-511, which is a potent inhibitor of SARS-CoV-2 with an EC90 of 0.47 μM.

Bemnifosbuvir Hemisulfate (AT-527) SARS-CoV inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 630.55

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Quality Control

Batch: E101301 DMSO]100 mg/mL]false]Water]100 mg/mL]false]Ethanol]100 mg/mL]false Purity: 99.37%
99.37

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (158.59 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL

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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 630.55 Formula

C24H33FN7O7P.1/2H2O4S

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 2241337-84-6 -- Storage of Stock Solutions

Synonyms RG-6422, RO 7496998 Smiles CNC1=C2N=C[N](C3OC(CO[P](=O)(NC(C)C(=O)OC(C)C)OC4=CC=CC=C4)C(O)C3(C)F)C2=NC(=N1)N.O[S](O)(=O)=O

Mechanism of Action

Targets/IC50/Ki
SARS-CoV-2
0.47 μM(EC90)
In vitro

Bemnifosbuvir Hemisulfate (AT-527) is the free base of AT-511. This compound inhibits replication of several human coronaviruses, such as HCoV-229E, HCoV-OC43, SARS-CoV in Huh-7 cells and HCoV-229E in BHK-21 cells. It can also inhibits SARS-CoV-2 replication in primary human airway epithelial (HAE) cells. In HBE and HNE cells, it can promote the host cells to form the pharmacologically active TP, AT-9010, to inhibit virus replication.
In vivo

After oral administration of Bemnifosbuvir Hemisulfate (AT-527) in nonhuman primates (NHPs), T-273 has the longest MRT at all dose levels, which indicate the long half-life of the active TP formed intracellularly. This compound is preferentially delivered to the liver, the target organ for HCV infection.
References

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05618314 Recruiting
Healthy Volunteer|Renal Impairment
Atea Pharmaceuticals Inc.
 October 17 2022 Phase 1
NCT05137626 Completed
Healthy Volunteers Study
Atea Pharmaceuticals Inc.|Hoffmann-La Roche
 November 3 2021 Phase 1
NCT04309734 Withdrawn
Hepatitis C|Hepatitis C Chronic|Chronic Hepatitis C|Hepatitis C Virus Infection|HCV Infection
Atea Pharmaceuticals Inc.
 October 2021 Phase 1|Phase 2
NCT04877769 Completed
Healthy Volunteer Study
Atea Pharmaceuticals Inc.|Hoffmann-La Roche
 April 25 2021 Phase 1

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