Ampicillin Trihydrate

For research use only.

Catalog No.S4148 Synonyms: NCI-C56086

1 publication

Ampicillin Trihydrate Chemical Structure

CAS No. 7177-48-2

Ampicillin Trihydrate (NCI-C56086) is a β-lactam antibiotic, which inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane.

Selleck's Ampicillin Trihydrate has been cited by 1 publication

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Biological Activity

Description Ampicillin Trihydrate (NCI-C56086) is a β-lactam antibiotic, which inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane.
Features More effective than levofloxacin and ciprofloxacin against Streptococcus pneumoniae.
beta-lactamase [1]
In vitro

Ampicillin Trihydrate (500 mg) results in rapid bactericidal effect for SP34, the concentrations declines to the limit of detection (100 CFU/mL) during the initial 6 hours, and regrowth is not observed over the remaining 18 hours. [1]

In vivo Ampicillin Trihydrate (10 mg/kg) results in half life (t 1/2) of 1.2 hours in goats and 2.48 hours in sheep, clearance (Cl) of 2921 mL/h·kg in goats and 262 mL/h·kg in sheep, ampicillin volume of distribution (Vdarea) of 5673 mL/kg in goats and 992 mL/kg in sheep. [2] Ampicillin Trihydrate (0.18 kg/kg-3 kg/kg) results in no dose-related mortality, normal body weights, no compound-related gross or histopathologic lesions and diarrhea in mice and rats. [3] Ampicillin in combination of norfloxacin, at a dose of 1g/L, maximally suppresses the numbers of cecal aerobic and anaerobic bacteria in ob/ob mice. Ampicillin in combination of norfloxacin results in a significant improvement in fasting glycemia and oral glucose tolerance reduced liver triglycerides and increased liver glycogen and concomitant reduction of plasma lipopolysaccharides in ob/ob mice. [4] Ampicillin trihydrate (20 mg/kg) treats five foals, the mean peak serum ampicillin concentration at two to three days old is 5.0 μg/mL at 1 hours after treatment, the mean peak serum concentration at 16 days to 21 days old is 2.7 μg/mL at 2 hours, the concentrations steadily declines and ampicillin is not detected in the serum from any of the foals by 24 hours, and serum clearance averages 17.7 mL/min/kg at two to three days and 35.8 mL/min/kg at 16 days to 21 days. [5]


Solubility (25°C)

In vitro DMSO 81 mg/mL (200.76 mM)
Water 2 mg/mL (4.95 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 403.45


CAS No. 7177-48-2
Storage powder
in solvent
Synonyms NCI-C56086
Smiles CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)O)C.O.O.O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04647331 Recruiting -- Infective Endocarditis Uppsala University June 1 2021 --
NCT03129620 Completed -- Hypothermia Drexel University March 2013 --
NCT01433757 Completed Drug: Ampicillin|Drug: Sugar pill DYT-1|Dystonia University of Florida|Tyler''s Hope for a Dystonia Cure Inc September 2011 Phase 1
NCT01118403 Withdrawn Drug: Sultamicillin Ventilator Associated Pneumonia Hospital Pablo Tobón Uribe March 2011 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID