For research use only.

Catalog No.S1401 Synonyms: GS-1278

15 publications

Tenofovir  Chemical Structure

Molecular Weight(MW): 287.21

Tenofovir blocks reverse transcriptase and hepatitis B virus infections.

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10mM (1mL in DMSO) USD 110 In stock
USD 97 In stock
USD 270 In stock
USD 570 In stock
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Selleck's Tenofovir has been cited by 15 publications

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  • The Nucleotide analog reverse-transcriptase inhibitor Tenofovir disoproxil fumarate was added to TZM-bl cells. Cells were inoculated with 0.05 ng mock-exposed and semen-exposed HIV, and 0.5 ng HIV as infectivity-matched control. Infection rates were measured 3 days post infection by measuring β-galactosidase or or 4 days post infection by measuring luciferase activities. The left panels show the mean enzyme activities ± standard deviation derived from triplicate infections. RLU/s: relative light units per second. Middle panels show normalized infection rates in which reporter enzyme activities obtained from infected cells in the absence of inhibitor were set at 100%. The right panels depict the calculated IC50 values. The number above the bar represents the fold-change in the IC50 derived from 0.05 ng semen-exposed relative to 0.05 or 0.5 ng mock-exposed virus infection. Ns, no statistically significant difference; **** p<0.0001; *** p<0.001 (unpaired t-test).

    Sci Transl Med, 2014, 6(262):262ra157.. Tenofovir purchased from Selleck.

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Biological Activity

Description Tenofovir blocks reverse transcriptase and hepatitis B virus infections.
Features Tenofovir disoproxil fumarate is the prodrug form of tenofovir.
Reverse transcriptase [1]
In vitro

Tenofovir reduces the viral cytopathic effect of HIV-1(IIIB), HIV-2(ROD) and HIV(EHO) with EC50 of 1.15 μg/mL, 1.12 μg/mL and 1.05 μg/mL in MT-4 cells. Tenofovir also reduces the viral cytopathic effect of SIV(mac251) , SIV(B670) ,SHIV(89.6) and SHIV(RTSHIV). [1] Tenofovir is uniquely active against multinucleoside-resistant HIV expressing the Q151M mutation, but shows reduced susceptibility to the T69S insertion mutations. [2] Tenofovir inhibits hepatitis B virus (HBV) activity in HepG2 2.2.15, HepAD38 and HepAD79 cells. [3] Tenofovir (4 μM) completely inhibits the growth of HIVIIIB in MT-2 cells. Tenofovir inhibits synthesis of negative strand strong-stop DNA with IC50 of 9 µM for wild-type RT, 6 µM for M184V RT and 50 µM for K65R RT. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MT2 cells M1j2S2Z2dmO2aX;uJIF{e2G7 Mm\MOUBl[Xm| NHvlepdCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJG1VOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYX\0[ZIhPSCmYYnzJIJ6KFiWVDDhd5NigSxiRVO1NF0xNjBzMzFOwG0> MlHYNlA1ODl5MkG=
human H9 cells NYLSe49XTnWwY4Tpc44h[XO|YYm= NIPFeGtCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIHPsZYRmKEZiaYPvcIF1\SB{M{O4JIlv\mWldHXkJIlvKGi3bXHuJGg6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36sJGVEPTB;MD6wOEDPxE1? NF;2bpQzOThyM{S2Ni=>
C3H/3T3 cells MkTISpVv[3Srb36gZZN{[Xl? MoTiOkBl[Xm| NYj3XVA1UW6qaXLpeIlwdiCxZjDteZJqdmVic3HyZ49u[SC4aYL1d{1qdmS3Y3XkJJRz[W6|Zn;ycYF1cW:wIH;mJI1wfXOnIHXtZpJ6dyCoaXLyc4Jt[XO2IFOzTE8{XDNiY3XscJMh[W[2ZYKgOkBl[Xm|LDDFR|UxRTBwMkOg{txO NI\FSpEyQDV3NkKwPS=>
CEM (human leukemia) cells MlrFSpVv[3Srb36gZZN{[Xl? MmrkRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXNTFiKFnJTWIqKGmwIFPFUUApcHWvYX6gcIV2c2WvaXGpJINmdGy|LDDFR|UxRTFwMjFOwG0> MmXENVQ2QDR7NU[=
MT-4 cells NUW3do9zTnWwY4Tpc44h[XO|YYm= NH3BSVFKdiC4aYTyc{BidnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZvMjDpckBOXC12IHPlcIx{NCCHQ{WwQVEvPCEQvF2= NGTlPWsyOTN{N{W4Oy=>
human bone marrow cells NF7lXZFEgXSxdH;4bYNqfHliYYPzZZk> MWGyOEBp MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDic45mKG2jcoLve{Bk\WyuczDh[pRmeiB{NDDodpMh[nliQl\VMWUh[XO|YYmsJGNEPTB;Mz61JO69VQ>? M3z6RlIxPDN7NkC5
C8166 cells MX\GeY5kfGmxbjDhd5NigQ>? NWLrS4p{PCCmYYnz NVPsdmU2SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhOTByIGTDTWQ2OCC5aXzkMZR6eGViSIXtZY4hcW2vdX7v[IVncWOrZX7jfUB3cXK3czD0fZBmKDJiUl;EJIlv\mWldHXkJIlvKEN6MU[2JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2Zic4nuZ5l1cWFiZn;ycYF1cW:wIHHmeIVzKDRiZHH5d{BjgSCvaXPyc5Nkd3CrYzDhcoFtgXOrczygSWM2OD15LkGg{txO NGjZN48zOThyM{S2Ni=>
mouse L1210 cells NHSyPXpEgXSxdH;4bYNqfHliYYPzZZk> Mn\1OFghcA>? NUPXb2pbS3m2b4P0ZZRq[yCjY4Tpeol1gSCjZ3HpcpN1KG2xdYPlJGwyOjFyIHPlcIx{KGGodHXyJFQ5KGi{czDifUBkd3WudHXyJINwfW62aX7nJIFv[Wy7c3nzMEBKSzVyPUGxJO69VQ>? MoO1NlQ3QDZyMUK=
human HepG2 cells NV;nPXNCTnWwY4Tpc44h[XO|YYm= NGfjS3I6KGSjeYO= Mkj1RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTIVx[XSrdHnzJGIhfmm{dYOgbY5n\WO2ZXSgbJVu[W5iSHXwS|Ih[2WubIOgZYZ1\XJiOTDkZZl{KGK7IF3UWEBie3OjeTygTWM2OD1zMj6zJO69VQ>? MV2xO|g5QDZ4Mh?=
human HeLa cells NFiwPWdEgXSxdH;4bYNqfHliYYPzZZk> MXW3NkBp MVnDfZRwe3SjdHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXMZUBk\WyuczDh[pRmeiB5MjDodpMh[nliY3;1cJRmeiClb4XueIlv\yCjbnHsfZNqeyxiSVO1NF0yPyEQvF2= NGLpXZUzPDZ6NkCxNi=>
CHO cells MmPtR5l1d3SxeHnjbZR6KGG|c3H5 M{TyZ|EzOCCq M{nEXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEOKTzDj[YxteyCjZoTldkAyOjBiaILzJIJ6KEOnbHytWIl1\XJiR3zvJIF{e2G7LDDDR|UxRTJzIN88US=> M2\yVlE6ODBzMUC4
MDCK2 cells MX3GeY5kfGmxbjDhd5NigQ>? NEHPSGIyOCEQvF2= MWLJcohq[mm2aX;uJI9nKGi3bXHuJG1TWDNiZYjwdoV{e2WmIHnuJG1FS0t{IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIlvfHKjY3XscJVt[XJiQ13GJIZtfW:{ZYPj[Y5k\SCjdDCxNEB2VSCkeTDDUWZFSSCjc4PhfS=> M3jGeVE4OTd{M{Gx
human HepG2 cells MmCzR5l1d3SxeHnjbZR6KGG|c3H5 M3vNXlE1KGSjeYO= MnLRR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyudXzhdkBFVkFicnXwcIlk[XSrb36gZYZ1\XJiMUSg[IF6ew>? MVKyNFQxQTd{MR?=

... Click to View More Cell Line Experimental Data

In vivo Tenofovir (30 mg/kg) completely prevents SIV infection in all macaques without toxicity. Tenofovir treatment reduces plasma viral RNA levels to undetectable, with parallel decreases in the infectivity of plasma and infectious cells in peripheral blood mononuclear cells and cerebrospinal fluid (CSF) and stabilization of CD4+ T-cell numbers. Tenofovir (30 mg/kg, s.c.) completely abrogates HIV infection via intravaginal exposure in pig-tailed macaques. [5]


Animal Research:[1]
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  • Animal Models: Macaques
  • Dosages: 30 mg/kg
  • Administration: Subcutaneously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 4 mg/mL warmed (13.92 mM)
Water 2 mg/mL (6.96 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% propylene glycol, 5% Tween 80, 65% D5W
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 287.21


CAS No. 147127-20-6
Storage powder
in solvent
Synonyms GS-1278
Smiles CC(C[N]1C=NC2=C(N)N=CN=C12)OC[P](O)(O)=O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04195776 Not yet recruiting Drug: Tenofovir Douche HIV/AIDS|HIV Prevention Johns Hopkins University|National Institute of Allergy and Infectious Diseases (NIAID)|CONRAD June 2020 Phase 1

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Reverse Transcriptase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID