4μ8C

For research use only.

Catalog No.S7272 Synonyms: IRE1 Inhibitor III

19 publications

4μ8C Chemical Structure

CAS No. 14003-96-4

4μ8C (IRE1 Inhibitor III) is a potent and selective IRE1 Rnase inhibitor with IC50 of 76 nM.

Size Price Stock Quantity  
USD 147 In stock
USD 470 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's 4μ8C has been cited by 19 publications

Purity & Quality Control

Choose Selective IRE1 Inhibitors

Biological Activity

Description 4μ8C (IRE1 Inhibitor III) is a potent and selective IRE1 Rnase inhibitor with IC50 of 76 nM.
Features IRE1 Rnase-selective inhibitor, used as a platform for developing new locally acting drugs.
Targets
IRE1 Rnase [1]
(Cell-free assay)
76 nM
In vitro

4μ8C blocks substrate(RIDD) access to the active site of IRE1 and selectively inactivates both Xbp1 splicing and IRE1-mediated mRNA degradation. IRE1 inhibition subsequently induces ER stress without measureable acute toxicity. [1] 4μ8C, as an IRE1 inhibitor, blocks IL-4, IL-5, and IL-13 production from CD4+ T cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SF21 cells NEmwWYhHfW6ldHnvckBie3OjeR?= NGPpfIE{OCCvaX7z MlLOTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCydYLpeIlvNUircz30ZYdo\WRiSWLFMVEhWk6jc3Wg[ZhxemW|c3XkJIlvKFOIMkGgZ4VtdHNidYPpcochYEKSLUGgVm5CKHO2ZX2gcI9weCCjczDzeYJ{fHKjdHWgbY5kfWKjdHXkJIZweiB|MDDtbY5{KHC{aX;yJJRwKHO3YoP0doF1\SCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkAzKGi{czDifUBHWkWWLYP1dJBz\XO|aX;uJIF{e2G7LDDJR|UxRTBwMkC2JO69VQ>? MVyyOFc1QTh4MR?=
human Jeko cells MUPGeY5kfGmxbjDhd5NigQ>? NX31e3pYOjRiaB?= MoezTY5pcWKrdHnvckBw\iC[QmCtNZMh\XiycnXzd4lwdiCrbjDoeY1idiCMZXvvJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCrbX31co9jdG:2dHnu[{BidmGueYPpd{whUUN3ME2xMlU4KM7:TR?= NHrvXWYzPDd2OUi2NS=>
human Mino cells M1PEcmZ2dmO2aX;uJIF{e2G7 MXKyOEBp MlrOTY5pcWKrdHnvckBw\iC[QmCtNZMh\XiycnXzd4lwdiCrbjDoeY1idiCPaX7vJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCrbX31co9jdG:2dHnu[{BidmGueYPpd{whUUN3ME2xMlYzKM7:TR?= MnjCNlQ4PDl6NkG=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:

[1]

- Collapse

In Vitro IRE1 RNase and RIDD Assays:

Analysis of radiolabeled Xbp1 substrate cleavage is performed as previously except that mammalian IRE1 reaction buffer is used. In vitro RIDD substrates are synthesized by in vitro transcription using the T7-MAXIscript Kit in the presence of 32P ATP or Cy5-UTP on templates isolated by RT-PCR from mouse Min6 cells (Ins2) or PCR from cloned XBP1 cDNA. The resulting products are gel purified to obtain full-length substrate. Reactions are then separated by 15% UREA-PAGE for analysis by phosphorimaging or by near-infrared imaging using the LI-COR Odyssey scanner.
Cell Research:

[1]

- Collapse
  • Cell lines: Wild-type MEFs
  • Concentrations: ~128 μM
  • Incubation Time: 24 hours
  • Method:

    Cells are seeded in phenol red-free cell culture medium in 96 or 24 well dishes at a density of 5 × 103 or 5 × 104 cells per well, respectively. Cultures are incubated for 16 h before treatment with 4μ8C for 24 h. Cultures are then analyzed by the addition of 200 μM WST1 and 10 μM phenazine metho-sulfate. After development of the reagent for 2 h at 37°C, the hydrolyzed dye is detected by absorbance at 450 nm, after subtracting background and absorbance at 595 nm. Alternatively, cell viability is determined by staining of the adherent culture with crystal violet. Quantitation of the dye uptake is analyzed by extensive washing of the stained cells with water and solublization of the crystal violet in methanol followed by absorbance measurements at 595 nm.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 19 mg/mL (93.05 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
For best results, use promptly after mixing.
0.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 204.18
Formula

C11H8O4

CAS No. 14003-96-4
Storage powder
in solvent
Synonyms IRE1 Inhibitor III
Smiles CC1=CC(=O)OC2=C1C=CC(=C2C=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy 4μ8C | 4μ8C supplier | purchase 4μ8C | 4μ8C cost | 4μ8C manufacturer | order 4μ8C | 4μ8C distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID