4-Aminobutyric acid (GABA)

For research use only.

Catalog No.S4700 Synonyms: 4-Aminobutanoic acid, GABA, Gamma-aminobutyric acid, Piperidic acid

4-Aminobutyric acid (GABA) Chemical Structure

CAS No. 56-12-2

4-Aminobutyric acid (4-Aminobutanoic acid, GABA, Gamma-aminobutyric acid, Piperidic acid) is a naturally occurring neurotransmitter with central nervous system (CNS) inhibitory activity.

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Biological Activity

Description 4-Aminobutyric acid (4-Aminobutanoic acid, GABA, Gamma-aminobutyric acid, Piperidic acid) is a naturally occurring neurotransmitter with central nervous system (CNS) inhibitory activity.
Targets
GABA receptor [1]
In vitro

γ-Aminobutyric acid (GABA) functions primarily as an inhibitory neurotransmitter in the mature central nervous system. The addition of GABA into the cell culture medium promoted the proliferation of GABRP-expressing PDAC cells, but not GABRP-negative cells, and GABAA receptor antagonists inhibited this growth-promoting effect by GABA. The HEK293 cells constitutively expressing exogenous GABRP revealed the growth-promoting effect of GABA treatment. GABA treatment in GABRP-positive cells increased intracellular Ca2+ levels and activated the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/Erk) cascade[1]. GABA exerts antidiabetic effects by acting on both the islet β-cells and immune system. Unlike in adult brain or islet α-cells in which GABA exerts hyperpolarizing effects, in islet β-cells, GABA produces membrane depolarization and Ca2+ influx, leading to the activation of PI3K/Akt-dependent growth and survival pathways[2].

In vivo GABA is the principal inhibitory neurotransmitter in the adult brain that has a parallel inhibitory role in the immune system. GABAergic medications are used to treat anxiety, alcohol withdrawal, epilepsy, and to induce sedation, and anesthesia. GABA is neuroprotective in animal models of stroke. GABA treatment decreases inflammatory cytokine production in peripheral macrophages. It decreases T cell autoimmunity and the development of inflammatory responses in the nonobese diabetic mouse model of type 1 diabetes[3]. In the adult brain, GABA induces a fast inhibition in neurons mainly through the GABAA receptor (GABAAR). GABA is produced by pancreatic β-cells. GABA released from β-cells can act on GABAAR in the α-cells, causing membrane hyperpolarization and hence suppressing glucagon secretion. GABA-treated mice showed higher circulating insulin, lower glucagon, nearly normal glycemia, improved metabolic conditions, and maintained close to normal glucose tolerance during a period of 53 d after STZ injections[2].

Protocol

Cell Research:[1]
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  • Cell lines: PDAC cell lines KLM-1, SUIT-2, KP-1N, PK-1, PK-45P, and PK-59
  • Concentrations: 0, 1, 10, 100 μmol/L
  • Incubation Time: 6 days
  • Method: GABRP-positive cell lines, KLM-1 and PK-45P, and GABRP-negative cell lines, PK-59 and KP-1N, are incubated with GABA or GABA receptor agonist Muscimol at serial concentration (0, 1, 10, 100 μmol/L) in appropriate medium supplemented with 1% FBS for 6 days. To inhibit the GABA-mediated pathway, cells are incubated with 250 μmol/L of GABAA receptor antagonist bicuculline methiodide or 1 mmol/L of GABAB receptor antagonist CGP-35348. After 6 days of exposure to either of these drugs, cell viability is measured by MTT assay as described above.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: Mice(CD1 mice) with STZ-induced diabetes
  • Dosages: 20 μmol per mouse
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Water 20 mg/mL (193.94 mM)
DMSO Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 103.12
Formula

C4H9NO2

CAS No. 56-12-2
Storage powder
in solvent
Synonyms 4-Aminobutanoic acid, GABA, Gamma-aminobutyric acid, Piperidic acid
Smiles C(CC(=O)O)CN

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04671836 Not yet recruiting Drug: Lorazepam 1Mg Tablet|Drug: Placebo Lorazepam|Neuroimaging Northwell Health January 2022 Early Phase 1
NCT04794335 Not yet recruiting -- Inflammatory Bowel Diseases University of Pittsburgh|National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) October 1 2021 --
NCT04831684 Not yet recruiting Drug: Placebo|Drug: GET73 Alcohol Use Disorder Medical University of South Carolina|National Institutes of Health (NIH)|National Institute on Alcohol Abuse and Alcoholism (NIAAA) September 15 2021 Phase 1|Phase 2
NCT04735172 Not yet recruiting Other: specific MRI Acquisition (NMR spectroscopy) at 7T Parkinson''s Disease University Hospital Clermont-Ferrand September 2021 Not Applicable
NCT04532190 Not yet recruiting Device: rTMS Attention-Deficit/Hyperactivity Disorder (ADHD) University of Calgary August 31 2021 Not Applicable
NCT04450550 Recruiting Device: VeNS device|Device: Sham VeNS device Parkinson Disease Neurovalens Ltd.|R D Gardi Medical College Ujjain July 26 2021 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID