Catalog No.S7153

10058-F4 Chemical Structure

Molecular Weight(MW): 249.35

10058-F4 is a c-Myc inhibitor that specificallly inhibits the c-Myc-Max interaction and prevents transactivation of c-Myc target gene expression.

Size Price Stock Quantity  
USD 170 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 21 Publications

Purity & Quality Control

Choose Selective c-Myc Inhibitors

Biological Activity

Description 10058-F4 is a c-Myc inhibitor that specificallly inhibits the c-Myc-Max interaction and prevents transactivation of c-Myc target gene expression.
c-Myc [1]
(Cell-free assay)
In vitro

10058-F4 inhibits growth of leukemic cells and dimerization of Myc and Max. 10058-F4 induces cell-cycle arrest and apoptosis of AML cells. 10058-F4 arrests AML cells at G0/G1 phase, downregulates c-Myc expression and upregulated CDK inhibitors, p21 and p27. Meanwhile, 10058-F4 induces apoptosis through activation of mitochondrial pathway shown by downregulation of Bcl-2, upregulation of Bax, release of cytoplasmic cytochrome C, and cleavage of caspase 3, 7, and 9. Furthermore, 10058-F4 also induces myeloid differentiation, possibly through activation of multiple transcription factors. Similarly, 10058-F4-induced apoptosis and differentiation could also be observed in primary AML cells. [1] 10058-F4 decreases c-Myc protein levels, inhibites proliferation of HepG2 cells likely through upregulation of cyclin-dependent kinase (cdk) inhibitor, p21WAF1 and lowers intracellular levels of [alpha]-fetoprotein (AFP). Treatment with 10058-F4 also downregulates human telomerase reverse transcriptase (hTERT) at the transcriptional level. In addition to inhibiting the proliferation of HepG2 cells, 10058-F4 enhances sensitivity to conventional chemotherapeutic agents, doxorubicin, 5-fluorouracil (5-FU) and cisplatin. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Nalm-6 MmTESpVv[3Srb36gZZN{[Xl? NHrhc2wxNTRyMPMAjeK2VQ>? NXTHWVY1PDhiaB?= MmrmdoVlfWOnZDD0bIUhdWW2YXLvcIlkKGGldHn2bZR6NCCLQ{WwQVQ{OCEQvF2= MmXvN|A6PTd{N{O=
Jurkat NH7Pd4VHfW6ldHnvckBie3OjeR?= NFvY[ZU3OCEQvF2= M3fTNFI1KGh? NU\jeJZL[y2PeXOg[ZhxemW|c3nvckBt\X[nbIOgeJJm[XSnZDD3bZRpKF[SQTCoNEwhOC56IHHu[EAyNjZibV2pJINwdWKrbnXkJJdqfGhiNkCg{txOKDFyMEW4MWY1KGSnY4LlZZNm\CCodYL0bIVzKGOxbYDhdoVlKHerdHigeIhmKGOxcoLld5BwdmSrbnegZ49vfHKxbIO= MVeyOVEzODd{Mx?=
CCRF-CEM NUPTTmlDTnWwY4Tpc44h[XO|YYm= MUe2NEDPxE1? NXjle2c3OjRiaB?= MUnjMW16[yCneIDy[ZN{cW:wIHzleoVteyC2cnXheIVlKHerdHigWnBCKChyLDCwMlgh[W6mIEGuOkBuVSliY3;tZolv\WRid3n0bEA3OCEQvF2gNVAxPThvRkSg[IVkemWjc3XkJIZ2enSqZYKgZ49ueGG{ZXSge4l1cCC2aHWgZ49zemW|cH;u[Ilv\yClb370do9tew>? NWjwPJJJOjVzMkC3NlM>
HL-60 NH76Z41HfW6ldHnvckBie3OjeR?= NGPnR2c3OCCjbnSgNVAxKM7:TR?= M1HJbVI1KGixdYLz M{X5SYRm[3KnYYPl[EBt\X[nbIOgc4Yh[y2PeXOgdJJwfGWrboO= MoLONVcxPDZ3Nke=
U937 MYPGeY5kfGmxbjDhd5NigQ>? NH;3Rmg3OCCjbnSgNVAxKM7:TR?= NGfGNGQzPCCqb4Xydy=> Mkjk[IVkemWjc3XkJIxmfmWuczDv[kBkNU27YzDwdo91\Wmwcx?= NHeySpMyPzB2NkW2Oy=>
NB4 MnniSpVv[3Srb36gZZN{[Xl? MWG2NEBidmRiMUCwJO69VQ>? NIjJPWkzPCCqb4Xydy=> NY\vNnlY\GWlcnXhd4VlKGyndnXsd{Bw\iClLV35Z{Bxem:2ZXnudy=> M3fBSFE4ODR4NU[3

... Click to View More Cell Line Experimental Data

In vivo Peak plasma 10058-F4 concentrations of approximately 300 μM are seen at 5 min and declined to below the detection limit at 360 min following a single iv dose. Plasma concentration versus time data are best approximated by a two-compartment, open, linear model. The highest tissue concentrations of 10058-F4 are found in fat, lung, liver, and kidney. Peak tumor concentrations of 10058-F4 are at least tenfold lower than peak plasma concentrations. Eight metabolites of 10058-F4 are identified in plasma, liver, and kidney. The terminal half-life of 10058-F4 is approximately 1 h, and the volume of distribution is >200 ml/kg. No significant inhibition of tumor growth is seen after i.v. treatment of mice with either 20 or 30 mg/kg 10058-F4.[3]


Cell Research:


+ Expand
  • Cell lines: HL-60, U937, and NB-4 cells
  • Concentrations: 0, 30, 60, 90, 120, 150 μM
  • Incubation Time: 72 h
  • Method:

    Cells, plated in 96-well plates (105/mL for cell lines and 5 × 105/mL for primary leukemic cells), are treated in triplicate with indicated concentrations of 10058-F4. At various time points, 20 μL 5 mg/mL MTT is added to each well. After incubation at 37°C for 3 hours, the MTT medium is removed and 100 μL DMSO lysis buffer is added. The number of viable cells is assessed by the percentage of absorbance of treated cells relative to that of solvent controls, using 570-nm wavelength on a spectrophotometer.

    (Only for Reference)
Animal Research:


+ Expand
  • Animal Models: PC-3 and DU145 xenografted SCID mice
  • Formulation: --
  • Dosages: 20 or 30 mg/kg
  • Administration: i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 49 mg/mL (196.51 mM)
Ethanol 4 mg/mL (16.04 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 249.35


CAS No. 403811-55-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

c-Myc Signaling Pathway Map

Tags: buy 10058-F4 | 10058-F4 supplier | purchase 10058-F4 | 10058-F4 cost | 10058-F4 manufacturer | order 10058-F4 | 10058-F4 distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID