Zofenopril calcium

Catalog No.S5712 Synonyms: SQ-26991

For research use only.

Zofenopril calcium (SQ-26991) is an angiotensin-converting enzyme (ACE) inhibitor that protects the heart and helps reduce high blood pressure.

Zofenopril calcium Chemical Structure

CAS No. 81938-43-4

Purity & Quality Control

Choose Selective RAAS Inhibitors

Other RAAS Products

Biological Activity

Description Zofenopril calcium (SQ-26991) is an angiotensin-converting enzyme (ACE) inhibitor that protects the heart and helps reduce high blood pressure.
ACE [1]
In vitro

In guinea pig ileum zofenopril inhibited the contractile response to angiotensin I and augmented the contractile response to bradykinin. The EC50 of zofenoprilat was in the nanomolar range (1-8 nM). In homogenates of aorta, brain, heart, lung, kidney, and serum of spontaneously hypertensive rats (SHRs), zofenoprilat inhibited ACE with a very similar potency (IC50: 0.8-2.8 nM). Zofenoprilat (0.01-1 mM) was shown to protect endothelial function. It abolished the proapoptotic effects of doxorubicin, promoted mitosis of bovine coronary venular endothelial cells (CVEC), enhanced concentration-dependently cell survival, and improved VEGF-induced proliferation of CVECs (kept 5 days in 0.1% serum to mimic a stress condition). Zofenoprilat (1-100 μM) also promoted angiogenesis in porcine coronary arteries. Zofenoprilat appears to favor proliferation of coronary endothelial cells, leading to angiogenesis by reversing apoptosis[1].

In vivo In conscious rats, dogs, and monkeys, oral administration of zofenopril (0.03-0.6 mg/kg) induces a dose-dependent inhibition of the pressor response to angiotensin I. It does not antagonize the effects of angiotensin II or other spasmogens. In 2K-1C rats, zofenopril produces a dose-dependent antihypertensive effect of long duration (>17 h). At 6.6 mg/kg, zofenopril lowered blood pressure by as much as 70 mmHg (220 to 150 mmHg). In SHRs, zofenopril has dose-dependent effects, lowering of blood pressure by 21-33 mmHg. At the highest dose used, zofenopril lowers blood pressure for at least 17 h. The effects of repeated administration of 22 mg/kg zofenopril b.i.d. were evaluated in SHRs. Systolic blood pressure (SBP) fell by 47 mmHg (188 to 141 mmHg) by day 14 of the study. The duration of the effect was longer than 12 h as identical SBP values were recorded at either 1 or 12 h after drug administration. Based on urinary excretion after oral and i.v. administration, the oral absorption of zofenopril was estimated to exceed 80% in rats and dogs and 70% in monkeys. The bioavailability of zofenoprilat after an oral dose of zofenopril was 100% in rats, >70% in dogs, and 50% in monkeys. Based on a comparison of AUC values after oral and i.v. administration, the oral absorption of [14C]zofenopril in dogs was 100% and the bioavailability of zofenoprilat was 93%. Following an oral administration of zofenopril, Tmax values for zofenoprilat were between 0.3-0.9 h and T1/2 values between 5–7 h for either of the three animal species. In dogs receiving zofenopril by intraarterial, intravenous, intraportal, or oral routes, 95% of the orally administered dose of zofenopril was hydrolyzed during the first pass[1].

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 5.33 mg/mL warmed
(5.94 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 897.17


CAS No. 81938-43-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(CSC(=O)C1=CC=CC=C1)C(=O)N2CC(CC2C(=O)[O-])SC3=CC=CC=C3.CC(CSC(=O)C1=CC=CC=C1)C(=O)N2CC(CC2C(=O)[O-])SC3=CC=CC=C3.[Ca+2]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Zofenopril calcium | Zofenopril calcium supplier | purchase Zofenopril calcium | Zofenopril calcium cost | Zofenopril calcium manufacturer | order Zofenopril calcium | Zofenopril calcium distributor