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VEGFD Rabbit mAb

Catalog No.: F2509

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:100 - 1:700 IF1:200 FCM1:100 - 1:500

Application
WB, IHC, IF, FCM

Source
Rabbit

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
40 kDa 65 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human cardiac muscle tissue; Human heart tissue; Human lung tissue; Human normal kidney tissue; Human skeletal muscle tissue; Rat lung tissue; Raji cell; HT1080 cell; Caco-2 cell; MCF-7 cell; NIH/3T3 cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
VEGFD Rabbit mAb recognizes endogenous levels of total VEGFD protein.

Uniprot ID
O43915

Clone
K3P16

Synonym(s)
FIGF, VEGFD, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor

Background
Vascular endothelial growth factor-D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein involved in the growth, remodeling, and maintenance of blood and lymphatic vessels. VEGF-D belongs to the VEGF family and shares the conserved cystine-knot motif, forming either non-covalent or covalent homodimers stabilized by intersubunit disulfide bonds. Each monomer consists of an antiparallel 4-stranded β-sheet, three connecting loops, and an N-terminal α-helix. VEGF-D acts as a ligand for VEGFR-2 and VEGFR-3, receptor tyrosine kinases predominantly expressed on endothelial cells, activating signaling pathways that promote cell proliferation, migration, tube formation, and vascular remodeling. It plays a critical role in pathological angiogenesis and lymphangiogenesis, and is implicated in various diseases including cancer, cardiovascular disorders, ocular diseases, and pulmonary lymphangioleiomyomatosis (LAM), where it serves as a diagnostic biomarker. VEGF-D's expression is regulated by the transcription factor c-Fos and its manipulation offers therapeutic potential in anti-angiogenic strategies and regenerative medicine

Background

Vascular endothelial growth factor-D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein involved in the growth, remodeling, and maintenance of blood and lymphatic vessels. VEGF-D belongs to the VEGF family and shares the conserved cystine-knot motif, forming either non-covalent or covalent homodimers stabilized by intersubunit disulfide bonds. Each monomer consists of an antiparallel 4-stranded β-sheet, three connecting loops, and an N-terminal α-helix. VEGF-D acts as a ligand for VEGFR-2 and VEGFR-3, receptor tyrosine kinases predominantly expressed on endothelial cells, activating signaling pathways that promote cell proliferation, migration, tube formation, and vascular remodeling. It plays a critical role in pathological angiogenesis and lymphangiogenesis, and is implicated in various diseases including cancer, cardiovascular disorders, ocular diseases, and pulmonary lymphangioleiomyomatosis (LAM), where it serves as a diagnostic biomarker. VEGF-D's expression is regulated by the transcription factor c-Fos and its manipulation offers therapeutic potential in anti-angiogenic strategies and regenerative medicine

References
[1] Achen MG, et al. Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):548-53. [2] Stacker SA, et al. Biomolecules. 2018 Jan 4;8(1):1.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%