Home Biosimilar Antibodies Immunology & Inflammation IL Receptor inhibitor Tocilizumab (anti-IL-6R)
For research use only. Not for use in humans.

Tocilizumab (anti-IL-6R)

Synonyms: MRA

Tocilizumab (anti-IL-6R) is a humanized monoclonal antibody that binds to the interleukin-6 receptor, MW: 148 KD.

Tocilizumab (anti-IL-6R) Chemical Structure

Click to purchase the isotype control of Tocilizumab (anti-IL-6R)

54 Citations

The Nobel Prize

17 Nobel Prize winners have used Selleck products

Shimon Sakaguchi

Nature Communications 2025,16, Article number:1325

David Baker

Dev Cell 2023, 58(20):2163-2180.e9.

David Julius

Cell 2017, 185-198.e16

Michael Houghton

Cell Chem Biol, 2020, 27(7):780-792.e5

Charles M. Rice

Cell 2018, 172(3):423-438.e25

Quality Control

Batch: Purity: 98.85% Protein concentration: 4.97mg/ml Endotoxin Level: ≤1 EU/mg
98.85

Specificity

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1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Mechanism of Action

Description
Tocilizumab (anti-IL-6R) is a humanized monoclonal antibody that binds to the interleukin-6 receptor, MW: 148 KD.
Features
Human IgG1
Targets/IC50/Ki
IL-6R
In vitro

After adding tocilizumab at different concentrations into HMO6 and CHME5 cells stimulated by F3-CM or with IL6, the expression levels of anti-inflammatory M2 markers (CD206 and Arg1) are decreased, as well as the levels of both phosphorylated JAK2 and STAT3.
Cell Research

HMO6 and CHME5 cells with control media or F3-CM alone or F3-CM were treated with Tocilizumab (2.5 μg/ml for 24 h) or with IL6 (200 ng/ml for 24 h) alone or IL6-supplemented with Tocilizumab. Western blot analysis of p-JAK2, JAK2, p-STAT3, and STAT3 in HMO6 and CHME5 cells were conducted.
In vivo

Tocilizumab can block JAK2/STAT3 activation and impede brain metastasis (BM) of A549-F3 cells in vivo.
Animal Research

Animal Models: Nude mice of brain metastasis models
Dosages: 5 mg/kg
Administration: i.p.
Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864012/
 
References

Product Details

CAS No. ‎375823-41-9
Isotype Human IgG1
Source CHO cells
Sterility 0.2 μM filtered
Formulation PBS buffer, pH 7.2
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Comparison of Clones for

*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

Tech Support

Handling Instructions

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Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.