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TIMP1 Rabbit mAb

Catalog No.: F0764

Application: Reactivity: Human, Monkey

Usage Information

Dilution
WB1:1000 IHC1:1000 - 1:4000

Application
WB, IHC

Reactivity
Human, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
26 kDa

Datasheet & SDS

Biological Description

Specificity
TIMP1 Rabbit mAb detects endogenous levels of total TIMP1 protein.

Clone
G13G11

Synonym(s)
Metalloproteinase inhibitor 1; Erythroid-potentiating activity (EPA); Fibroblast collagenase inhibitor (Collagenase inhibitor); Tissue inhibitor of metalloproteinases 1 (TIMP-1); TIMP1; CLGI; TIMP

Background
TIMP1(Tissue inhibitor of metalloproteinases-1) is a soluble is a pleiotropic glycoprotein of approximately 28 kDa (184 amino acids) encoded by the TIMP1 gene on chromosome Xp11.3–p11.23, and is a founding member of the TIMP family (TIMP-1 to TIMP-4), promoting both tumor-suppressive and tumor-promoting activities. Structurally, it consists of two distinct domains stabilized by 12 conserved cysteine residues forming disulfide bonds: an N-terminal wedge-shaped domain that binds and inhibits matrix metalloproteinases (MMPs) in a 1:1 stoichiometric ratio, thereby regulating extracellular matrix remodeling, and a C-terminal domain that mediates non-MMP-inhibitory functions, notably interacting with the cell surface receptor CD63 to activate pro-oncogenic signaling. TIMP-1 is expressed in a variety of tissues and secreted into body fluids, with elevated levels frequently observed in cancers such as gastric, breast, and colon cancer, often correlating with advanced disease stage and poor prognosis. Functionally, beyond its tumor-suppressive role via MMP inhibition, TIMP-1 exerts pleiotropic effects including promotion of cell proliferation, survival, epithelial-to-mesenchymal transition (EMT), and therapy resistance through CD63/integrin β1-mediated signaling, highlighting its dual role in cancer biology.

Background

TIMP1(Tissue inhibitor of metalloproteinases-1) is a soluble is a pleiotropic glycoprotein of approximately 28 kDa (184 amino acids) encoded by the TIMP1 gene on chromosome Xp11.3–p11.23, and is a founding member of the TIMP family (TIMP-1 to TIMP-4), promoting both tumor-suppressive and tumor-promoting activities. Structurally, it consists of two distinct domains stabilized by 12 conserved cysteine residues forming disulfide bonds: an N-terminal wedge-shaped domain that binds and inhibits matrix metalloproteinases (MMPs) in a 1:1 stoichiometric ratio, thereby regulating extracellular matrix remodeling, and a C-terminal domain that mediates non-MMP-inhibitory functions, notably interacting with the cell surface receptor CD63 to activate pro-oncogenic signaling. TIMP-1 is expressed in a variety of tissues and secreted into body fluids, with elevated levels frequently observed in cancers such as gastric, breast, and colon cancer, often correlating with advanced disease stage and poor prognosis. Functionally, beyond its tumor-suppressive role via MMP inhibition, TIMP-1 exerts pleiotropic effects including promotion of cell proliferation, survival, epithelial-to-mesenchymal transition (EMT), and therapy resistance through CD63/integrin β1-mediated signaling, highlighting its dual role in cancer biology.

References
https://pubmed.ncbi.nlm.nih.gov/32034211/ https://pubmed.ncbi.nlm.nih.gov/27644693/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%