Datasheet

Biological Description

Specificity	TIMP1 Rabbit mAb detects endogenous levels of total TIMP1 protein.
Background	TIMP1(Tissue inhibitor of metalloproteinases-1) is a soluble is a pleiotropic glycoprotein of approximately 28 kDa (184 amino acids) encoded by the TIMP1 gene on chromosome Xp11.3–p11.23, and is a founding member of the TIMP family (TIMP-1 to TIMP-4), promoting both tumor-suppressive and tumor-promoting activities. Structurally, it consists of two distinct domains stabilized by 12 conserved cysteine residues forming disulfide bonds: an N-terminal wedge-shaped domain that binds and inhibits matrix metalloproteinases (MMPs) in a 1:1 stoichiometric ratio, thereby regulating extracellular matrix remodeling, and a C-terminal domain that mediates non-MMP-inhibitory functions, notably interacting with the cell surface receptor CD63 to activate pro-oncogenic signaling. TIMP-1 is expressed in a variety of tissues and secreted into body fluids, with elevated levels frequently observed in cancers such as gastric, breast, and colon cancer, often correlating with advanced disease stage and poor prognosis. Functionally, beyond its tumor-suppressive role via MMP inhibition, TIMP-1 exerts pleiotropic effects including promotion of cell proliferation, survival, epithelial-to-mesenchymal transition (EMT), and therapy resistance through CD63/integrin β1-mediated signaling, highlighting its dual role in cancer biology.

Specificity

TIMP1 Rabbit mAb detects endogenous levels of total TIMP1 protein.

Background

TIMP1(Tissue inhibitor of metalloproteinases-1) is a soluble is a pleiotropic glycoprotein of approximately 28 kDa (184 amino acids) encoded by the TIMP1 gene on chromosome Xp11.3–p11.23, and is a founding member of the TIMP family (TIMP-1 to TIMP-4), promoting both tumor-suppressive and tumor-promoting activities. Structurally, it consists of two distinct domains stabilized by 12 conserved cysteine residues forming disulfide bonds: an N-terminal wedge-shaped domain that binds and inhibits matrix metalloproteinases (MMPs) in a 1:1 stoichiometric ratio, thereby regulating extracellular matrix remodeling, and a C-terminal domain that mediates non-MMP-inhibitory functions, notably interacting with the cell surface receptor CD63 to activate pro-oncogenic signaling. TIMP-1 is expressed in a variety of tissues and secreted into body fluids, with elevated levels frequently observed in cancers such as gastric, breast, and colon cancer, often correlating with advanced disease stage and poor prognosis. Functionally, beyond its tumor-suppressive role via MMP inhibition, TIMP-1 exerts pleiotropic effects including promotion of cell proliferation, survival, epithelial-to-mesenchymal transition (EMT), and therapy resistance through CD63/integrin β1-mediated signaling, highlighting its dual role in cancer biology.

Usage Information

Application

WB, IHC

Dilution

WB	IHC
1:1000	1:1000 - 1:4000

Reactivity

Human, Monkey

Source

Rabbit

MW

26 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/32034211/
https://pubmed.ncbi.nlm.nih.gov/27644693/

TIMP1 Rabbit mAb

Biological Description

Usage Information

References

Application Data