TC10 Rabbit mAb

Catalog No.: F3952

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000-1:10000 IF1:250 - 1:500

Application
WB, IF

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
23 kDa 25 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
TC10 Rabbit mAb detects endogenous levels of total TC10 protein.

Clone
D5M19

Synonym(s)
ARHQ, RASL7A, TC10, RHOQ, Rho-related GTP-binding protein RhoQ, Ras-like protein TC10, Ras-like protein family member 7A

Background
TC10 is a small GTP-binding protein of the Rho family, closely related to Cdc42, that functions as a molecular switch cycling between inactive GDP- and active GTP-bound states to regulate actin cytoskeleton dynamics and signaling. Structurally, it contains conserved G domains for nucleotide binding and hydrolysis, a unique N-terminal extension, and a C-terminal CAAX box for prenylation, which anchors it to plasma membrane microdomains essential for its activity. TC10 is primarily expressed in insulin-responsive tissues such as adipose and muscle, where it plays a key role in insulin signaling. Functionally, active TC10 regulates actin remodeling by depolymerizing cortical actin and promoting perinuclear actin polymerization, interacts with proteins such as N-WASP, profilin, and COPI coat components, and modulates GLUT4 vesicle trafficking, transcriptional responses (e.g., JNK, NF-κB, SRF), and cell growth processes.

Background

TC10 is a small GTP-binding protein of the Rho family, closely related to Cdc42, that functions as a molecular switch cycling between inactive GDP- and active GTP-bound states to regulate actin cytoskeleton dynamics and signaling. Structurally, it contains conserved G domains for nucleotide binding and hydrolysis, a unique N-terminal extension, and a C-terminal CAAX box for prenylation, which anchors it to plasma membrane microdomains essential for its activity. TC10 is primarily expressed in insulin-responsive tissues such as adipose and muscle, where it plays a key role in insulin signaling. Functionally, active TC10 regulates actin remodeling by depolymerizing cortical actin and promoting perinuclear actin polymerization, interacts with proteins such as N-WASP, profilin, and COPI coat components, and modulates GLUT4 vesicle trafficking, transcriptional responses (e.g., JNK, NF-κB, SRF), and cell growth processes.

References
https://pubmed.ncbi.nlm.nih.gov/12134073/ https://pubmed.ncbi.nlm.nih.gov/10445846/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%