Datasheet

Biological Description

Specificity	TC10 Rabbit mAb detects endogenous levels of total TC10 protein.
Background	TC10 is a small GTP-binding protein of the Rho family, closely related to Cdc42, that functions as a molecular switch cycling between inactive GDP- and active GTP-bound states to regulate actin cytoskeleton dynamics and signaling. Structurally, it contains conserved G domains for nucleotide binding and hydrolysis, a unique N-terminal extension, and a C-terminal CAAX box for prenylation, which anchors it to plasma membrane microdomains essential for its activity. TC10 is primarily expressed in insulin-responsive tissues such as adipose and muscle, where it plays a key role in insulin signaling. Functionally, active TC10 regulates actin remodeling by depolymerizing cortical actin and promoting perinuclear actin polymerization, interacts with proteins such as N-WASP, profilin, and COPI coat components, and modulates GLUT4 vesicle trafficking, transcriptional responses (e.g., JNK, NF-κB, SRF), and cell growth processes.

Specificity

TC10 Rabbit mAb detects endogenous levels of total TC10 protein.

Background

TC10 is a small GTP-binding protein of the Rho family, closely related to Cdc42, that functions as a molecular switch cycling between inactive GDP- and active GTP-bound states to regulate actin cytoskeleton dynamics and signaling. Structurally, it contains conserved G domains for nucleotide binding and hydrolysis, a unique N-terminal extension, and a C-terminal CAAX box for prenylation, which anchors it to plasma membrane microdomains essential for its activity. TC10 is primarily expressed in insulin-responsive tissues such as adipose and muscle, where it plays a key role in insulin signaling. Functionally, active TC10 regulates actin remodeling by depolymerizing cortical actin and promoting perinuclear actin polymerization, interacts with proteins such as N-WASP, profilin, and COPI coat components, and modulates GLUT4 vesicle trafficking, transcriptional responses (e.g., JNK, NF-κB, SRF), and cell growth processes.

Usage Information

Application

WB, IF

Dilution

WB	IF
1:1000-1:10000	1:250 - 1:500

Reactivity

Human

Source

Rabbit

MW

23 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/12134073/
https://pubmed.ncbi.nlm.nih.gov/10445846/

TC10 Rabbit mAb

Biological Description

Usage Information

References

Application Data