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TAZ Rabbit mAb

Catalog No.: F1356

Application: Reactivity: Human, Mouse, Monkey

Usage Information

Dilution
WB1:1000 IP1:50 IHC1:200 - 1:800 CHIP1:50

Application
WB, IP, IHC, ChIP

Reactivity
Human, Mouse, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
55 kDa

Datasheet & SDS

Biological Description

Specificity
TAZ Rabbit mAb detects endogenous levels of total TAZ protein.

Clone
B16J7

Synonym(s)
WW domain-containing transcription regulator protein 1; Transcriptional coactivator with PDZ-binding motif; WWTR1; TAZ 2

Background
TAZ (transcriptional co-activator with PDZ-binding motif), also known as WWTR1, is a multifunctional transcriptional coactivator that regulates gene expression, particularly downstream of the Hippo signaling pathway. As a member of the WW domain-containing protein family, TAZ features a WW domain that enables interactions with proline-rich proteins and a C-terminal PDZ-binding motif, which binds to PDZ-domain-containing scaffolding proteins, influencing its localization and activity. TAZ contains a transcriptional activation domain and regions that facilitate dimerization and interactions with transcription factors, such as the TEAD family and SMADs. TAZ activity is regulated by phosphorylation through Hippo pathway kinases (LATS1/2), which promote binding to 14-3-3 proteins, resulting in cytoplasmic retention and degradation, inhibiting its transcriptional co-activation capacity. Upon dephosphorylation, TAZ translocates to the nucleus where it co-activates genes involved in cell proliferation, stem cell maintenance, organ size control, and tissue regeneration. TAZ also participates in processes such as epithelial-mesenchymal transition (EMT), mechanotransduction, and integrates signals from Wnt and TGF-β pathways. Dysregulation of TAZ/WWTR1 expression or activity has been linked to cancer progression, metastasis, and therapy resistance.

Background

TAZ (transcriptional co-activator with PDZ-binding motif), also known as WWTR1, is a multifunctional transcriptional coactivator that regulates gene expression, particularly downstream of the Hippo signaling pathway. As a member of the WW domain-containing protein family, TAZ features a WW domain that enables interactions with proline-rich proteins and a C-terminal PDZ-binding motif, which binds to PDZ-domain-containing scaffolding proteins, influencing its localization and activity. TAZ contains a transcriptional activation domain and regions that facilitate dimerization and interactions with transcription factors, such as the TEAD family and SMADs. TAZ activity is regulated by phosphorylation through Hippo pathway kinases (LATS1/2), which promote binding to 14-3-3 proteins, resulting in cytoplasmic retention and degradation, inhibiting its transcriptional co-activation capacity. Upon dephosphorylation, TAZ translocates to the nucleus where it co-activates genes involved in cell proliferation, stem cell maintenance, organ size control, and tissue regeneration. TAZ also participates in processes such as epithelial-mesenchymal transition (EMT), mechanotransduction, and integrates signals from Wnt and TGF-β pathways. Dysregulation of TAZ/WWTR1 expression or activity has been linked to cancer progression, metastasis, and therapy resistance.

References
https://pubmed.ncbi.nlm.nih.gov/22003437/ https://pubmed.ncbi.nlm.nih.gov/30976198/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%