Datasheet

Biological Description

Specificity	TAZ Rabbit mAb detects endogenous levels of total TAZ protein.
Background	TAZ (transcriptional co-activator with PDZ-binding motif), also known as WWTR1, is a multifunctional transcriptional coactivator that regulates gene expression, particularly downstream of the Hippo signaling pathway. As a member of the WW domain-containing protein family, TAZ features a WW domain that enables interactions with proline-rich proteins and a C-terminal PDZ-binding motif, which binds to PDZ-domain-containing scaffolding proteins, influencing its localization and activity. TAZ contains a transcriptional activation domain and regions that facilitate dimerization and interactions with transcription factors, such as the TEAD family and SMADs. TAZ activity is regulated by phosphorylation through Hippo pathway kinases (LATS1/2), which promote binding to 14-3-3 proteins, resulting in cytoplasmic retention and degradation, inhibiting its transcriptional co-activation capacity. Upon dephosphorylation, TAZ translocates to the nucleus where it co-activates genes involved in cell proliferation, stem cell maintenance, organ size control, and tissue regeneration. TAZ also participates in processes such as epithelial-mesenchymal transition (EMT), mechanotransduction, and integrates signals from Wnt and TGF-β pathways. Dysregulation of TAZ/WWTR1 expression or activity has been linked to cancer progression, metastasis, and therapy resistance.

Specificity

TAZ Rabbit mAb detects endogenous levels of total TAZ protein.

Background

TAZ (transcriptional co-activator with PDZ-binding motif), also known as WWTR1, is a multifunctional transcriptional coactivator that regulates gene expression, particularly downstream of the Hippo signaling pathway. As a member of the WW domain-containing protein family, TAZ features a WW domain that enables interactions with proline-rich proteins and a C-terminal PDZ-binding motif, which binds to PDZ-domain-containing scaffolding proteins, influencing its localization and activity. TAZ contains a transcriptional activation domain and regions that facilitate dimerization and interactions with transcription factors, such as the TEAD family and SMADs. TAZ activity is regulated by phosphorylation through Hippo pathway kinases (LATS1/2), which promote binding to 14-3-3 proteins, resulting in cytoplasmic retention and degradation, inhibiting its transcriptional co-activation capacity. Upon dephosphorylation, TAZ translocates to the nucleus where it co-activates genes involved in cell proliferation, stem cell maintenance, organ size control, and tissue regeneration. TAZ also participates in processes such as epithelial-mesenchymal transition (EMT), mechanotransduction, and integrates signals from Wnt and TGF-β pathways. Dysregulation of TAZ/WWTR1 expression or activity has been linked to cancer progression, metastasis, and therapy resistance.

Usage Information

Application

WB, IP, IHC, ChIP

Dilution

WB	IP	IHC	CHIP
1:1000	1:50	1:200 - 1:800	1:50

Reactivity

Human, Mouse, Monkey

Source

Rabbit

MW

55 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/22003437/
https://pubmed.ncbi.nlm.nih.gov/30976198/

TAZ Rabbit mAb

Biological Description

Usage Information

References

Application Data