Smad1 (K19G18) Rabbit mAb

Catalog No.: F3338

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:1000 IF1:100 FCM1:100

Application
WB, IF, FCM

Source
Rabbit

Reactivity
Human, Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
52 kDa 55-65 kDa, 58 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Mouse brain; Mouse skeletal muscle; Mouse stomach; HEK-293 cell; A-673 cell; NIH/3T3 cell; C2C12 cell; A549 cell; HeLa cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Smad1 (K19G18) Rabbit mAb detects endogenous levels of total SMAD1 protein.

Uniprot ID
Q15797

Clone
K19G18

Synonym(s)
BSP1, MADH1, MADR1, SMAD1, Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, Transforming growth factor-beta-signaling protein 1, SMAD 1, Smad1, hSMAD1, BSP-1

Background
SMAD1 is a receptor-regulated SMAD (R-SMAD) protein and a key downstream effector of the BMP (bone morphogenetic protein) signaling pathway. Structurally, it comprises an N-terminal Mad homology 1 (MH1) domain responsible for DNA binding, a central proline-rich linker, and a C-terminal MH2 domain that mediates receptor interaction and transcriptional activity. Activation of SMAD1 occurs through phosphorylation at a conserved Ser-X-Ser motif by BMP type I receptors. SMAD1 is widely expressed during embryonic development and in specific adult tissues, particularly in regions undergoing organogenesis such as bone and cartilage. Functionally, SMAD1 regulates gene expression programs involved in tissue differentiation, especially in osteogenic and chondrogenic precursors, and plays a critical role in development processes that involve mesenchyme-epithelial interactions.

Background

SMAD1 is a receptor-regulated SMAD (R-SMAD) protein and a key downstream effector of the BMP (bone morphogenetic protein) signaling pathway. Structurally, it comprises an N-terminal Mad homology 1 (MH1) domain responsible for DNA binding, a central proline-rich linker, and a C-terminal MH2 domain that mediates receptor interaction and transcriptional activity. Activation of SMAD1 occurs through phosphorylation at a conserved Ser-X-Ser motif by BMP type I receptors. SMAD1 is widely expressed during embryonic development and in specific adult tissues, particularly in regions undergoing organogenesis such as bone and cartilage. Functionally, SMAD1 regulates gene expression programs involved in tissue differentiation, especially in osteogenic and chondrogenic precursors, and plays a critical role in development processes that involve mesenchyme-epithelial interactions.

References
[1] BabuRajendran N, et al. Nucleic Acids Res. 2010 Jun;38(10):3477-88. [2] Chaikuad A, et al. Cold Spring Harb Perspect Biol. 2016 Nov 1;8(11):a022111.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%