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Biological Description
| Specificity | Smad1 (K19G18) Rabbit mAb detects endogenous levels of total SMAD1 protein. |
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| Background | SMAD1 is a receptor-regulated SMAD (R-SMAD) protein and a key downstream effector of the BMP (bone morphogenetic protein) signaling pathway. Structurally, it comprises an N-terminal Mad homology 1 (MH1) domain responsible for DNA binding, a central proline-rich linker, and a C-terminal MH2 domain that mediates receptor interaction and transcriptional activity. Activation of SMAD1 occurs through phosphorylation at a conserved Ser-X-Ser motif by BMP type I receptors. SMAD1 is widely expressed during embryonic development and in specific adult tissues, particularly in regions undergoing organogenesis such as bone and cartilage. Functionally, SMAD1 regulates gene expression programs involved in tissue differentiation, especially in osteogenic and chondrogenic precursors, and plays a critical role in development processes that involve mesenchyme-epithelial interactions. |
Usage Information
| Application | WB, IF, FCM | Dilution |
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| Reactivity | Human, Mouse | ||||||||
| Source | Rabbit | MW | 52 kDa | ||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3 | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||
References
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