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For research use only.

Catalog No.S8531

PF-06650833  Chemical Structure

CAS No. 1817626-54-2

PF-06650833 is a potent, selective inhibitor of IRAK4 with an IC50 of 0.2 nM.

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Choose Selective IRAK Inhibitors

Biological Activity

Description PF-06650833 is a potent, selective inhibitor of IRAK4 with an IC50 of 0.2 nM.
IRAK4 [1]
(Cell-free assay)
0.2 nM
In vitro

The kinome selectivity profile of PF-06650833 is assessed in a panel of 278 kinases(Invitrogen) at 200 nM inhibitor concentration using the ATP Km for each kinase. Approximately 100% inhibition is observed for IRAK4 while greater than 70% inhibition is observed for the following kinases, in order of potency: IRAK1, MNK2, LRRK2, CLK4, and CK1γ1. Lactam PF-06650833 is subsequently assessed in a whole cell functional VEGF2R assay (PAE-KDR cell line). No activity was observed at concentrations up to and including 30 µM. [1].

In vivo

PF-06650833 significantly inhibits LPS-induced TNF in a dose dependent manner. Mean exposures of PF-06650833 in plasma are 2.1 nM, 7.7 nM, 19 nM and 150 nM free, respectively, at 2.5 hours after oral administration of PF-06650833 at 0.3 mg/kg, 1 mg/kg, 3 mg/kg and 30 mg/kg. The fraction unbound in rat plasma of PF-06650833 is 0.3.[1]


Animal Research:


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  • Animal Models: Male Sprague Dawley rats
  • Dosages: 0.1 mg/kg, 1 mg/kg, 3 mg/kg, 30 mg/kg
  • Administration: Oral
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 72 mg/mL (199.24 mM)
Ethanol 3 mg/mL (8.3 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 361.37


CAS No. 1817626-54-2
Storage powder
in solvent
Synonyms N/A
Smiles CCC1C(NC(=O)C1F)COC2=NC=CC3=CC(=C(C=C32)OC)C(=O)N

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID