Catalog No.S9731

For research use only.

PF-00835231 is a 3CLpro (Mpro) inhibitor that may targets SARS-CoV-2 protease 3CLpro as a potential new treatment for COVID-19.

PF-00835231 Chemical Structure

CAS No. 870153-29-0

Selleck's PF-00835231 has been cited by 4 Publications

Purity & Quality Control

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Biological Activity

Description PF-00835231 is a 3CLpro (Mpro) inhibitor that may targets SARS-CoV-2 protease 3CLpro as a potential new treatment for COVID-19.
3CLpro [1]
In vitro

A time-of-drug-addition approach delineates the timing of early SARS-CoV-2 life cycle steps in A549+ACE2 cells and validates PF-00835231's early time of action. In a model of the human polarized airway epithelium, PF-00835231 potently inhibits SARS-CoV-2 at low micromolar concentrations. PF-00835231 exhibits the potential as an effective SARS-CoV-2 antiviral.[1]

Protocol (from reference)

Cell Research:


  • Cell lines: A549 cells, human airway epithelial cultures (HAEC)
  • Concentrations: 1 nM-10 μM
  • Incubation Time: 0-4 h
  • Method:

    A549+ACE2 cells are seeded into black wall 96-well plates at 70% confluency. The next day, media is removed and replaced with complete media containing compound (PF-00835231)/carrier two hours prior to infection. Cells are then infected at 0.425 multiplicity of infection (MOI), based on Vero E6 titer, at 37°C. 1 hour post virus addition, virus is removed, and media containing compound/carrier is added. At 24 and 48 hours post infection, cells are fixed by submerging in 10% formalin solution for 30-45 min. After fixation, cells are washed once with H2O to remove excess formalin.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 472.53


CAS No. 870153-29-0
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05050682 Completed Drug: PF-07304814 Healthy Pfizer October 7 2021 Phase 1
NCT04627532 Completed Drug: PF-07304814|Drug: Placebo Healthy Pfizer October 23 2020 Phase 1
NCT04535167 Completed Drug: PF-07304814|Drug: Placebo Viral Disease Pfizer September 9 2020 Phase 1

(data from, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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