Necrosulfonamide

Catalog No.S8251

For research use only.

Necrosulfonamide is a very specific and potent necrosis inhibitor and blocks mixed lineage kinase domain-like protein (MLKL).

Necrosulfonamide Chemical Structure

CAS No. 1360614-48-7

Selleck's Necrosulfonamide has been cited by 61 publications

Purity & Quality Control

Choose Selective Mixed Lineage Kinase Inhibitors

Biological Activity

Description Necrosulfonamide is a very specific and potent necrosis inhibitor and blocks mixed lineage kinase domain-like protein (MLKL).
Targets
necrosis(MLKL) [1]
()
In vitro

Necrosulfonamide inhibits MLKL-mediated Necrosis by blocking its N-terminal CC domain function. It blocks necrosis downstream of RIP3 activation. Necrosulfonamide has no effect on apoptosis induced by TNF-α plus Smac mimetic in non-RIP3-expressing Panc-1 cells, even at 5 μM concentration. Necrosulfonamide efficiently blocks necrosis in human cells, but not mouse cells. The reason for the species specificity of necrosulfonamide is that the cysteine at residue 86 in human MLKL that is covalently modified by necrosulfonamide is replaced by a tryptophan residue in mouse MLKL(mixed lineage kinase domain-like protein)[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
IEC-6 cells M1fZPWZ2dmO2aX;uJIF{e2G7 NETS[ZUyOCEQvF2= MoDTOFAhdWmw MoXFVoVlfWOnZDDj[YxtKH[rYXLpcIl1gSC5YYOg[pVzfGincjDh[4dz[X[jdHXkJIJ6KGmwaHnibZRqd25ib3[gUWxMVCC3c3nu[{Bv\WO{b4P1cIZwdmGvaXTl Mne2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl7M{W1NlkoRjJ7OUO1OVI6RC:jPh?=
MDA-MB-231 NGfLeI1E\WyuII\pZYJqdGm2eTDhd5NigQ>? MoTBNlAh|ryP MYO0PEBp NXXEVmxOeHKndnXueEBkgXO2aX7lMZN1[XK4YYTpc44ucW6mdXPl[EBk\WyuIHTlZZRp MoK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|OEOxNFQoRjJ7M{izNVA1RC:jPh?=
Hs 578T MWPD[YxtKH[rYXLpcIl1gSCjc4PhfS=> NHThcmEzOCEQvF2= Mlu5OFghcA>? MVHwdoV3\W62IHP5d5RqdmVvc4ThdpZifGmxbj3pcoR2[2WmIHPlcIwh\GWjdHi= MmnSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|OEOxNFQoRjJ7M{izNVA1RC:jPh?=
HCC 1937 MVHD[YxtKH[rYXLpcIl1gSCjc4PhfS=> NFjNWnczOCEQvF2= MWS0PEBp MmnUdJJmfmWwdDDjfZN1cW6nLYP0ZZJ3[XSrb36tbY5lfWOnZDDj[YxtKGSnYYTo NHi1Z5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUO4N|ExPCd-MkmzPFMyODR:L3G+
U937 NVPOc20zTnWwY4Tpc44h[XO|YYm= NYjlT3Z7OC1{LkWg{txO MXWyNEBp MX3OV2EhemWmdXPl[EB1cGVicnH0bY8hd2ZiUFmgdI9{cXSrdnWgZ4VtdHNiaX6gZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDmZZNpcW:wIHnu[IVx\W6mZX70cJkhd2ZidHjlJJdigSCxZjDj[YxtKGSnYYToJJRzcWepZYKgLIVqfGincjDUVmFKVCCxcjDTWHMqKHWwZHXyJINie3Cjc3WtZ49ueHKxbXnz[YQh[2:wZHn0bY9vNg>? NGfN[Zc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki2NFA{Pyd-MkK4OlAxOzd:L3G+
HeLa MlHXNlAhdk1? MnPtR49ueGW2aYTpeoUh[mmwZHnu[{Bi\m[rbnn0fUB1dyCPeXOteIFo\2WmIF3MT2whMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCKZVzhJINmdGy|IHH0JFIxKG6PIHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqeyCrbjDwdoV{\W6lZTDv[kBjcW:2aX6tcIFj\WynZDDwc5NqfGm4ZTDwdo9j\Q>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlIxQTF6Lze+R4hGVUKOPD;hQi=>
Assay
Methods Test Index PMID
Western blot pMLKL / MLKL 29500427

Protocol (from reference)

Cell Research:[3]
  • Cell lines: HT-29 cells
  • Concentrations: 1 μM
  • Incubation Time: 8 or 12 hrs
  • Method: Necrosis inhibitors induce diverse effects on MLKL phosphorylation. HT-29 cells are treated with T/S/Z with or without necrosis inhibitors for 12 hr or 8 hr. The number of dead cells is determined by measuring released protease activity in culture medium. The whole-cell extracts are prepared and analyzed by western blotting. The final concentrations of 10 μM necrostatin-1 or 1 μM necrosulfonamide are used to block necrosis.

Solubility (25°C)

In vitro

DMSO 92 mg/mL
(199.36 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 461.47
Formula

C18H15N5O6S2

CAS No. 1360614-48-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=NC=CN=C1NS(=O)(=O)C2=CC=C(C=C2)NC(=O)C=CC3=CC=C(S3)[N+](=O)[O-]

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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