ML130 (Nodinitib-1)

For research use only.

Catalog No.S2863

8 publications

ML130 (Nodinitib-1) Chemical Structure

CAS No. 799264-47-4

ML130 (Nodinitib-1) is a potent and selective inhibitor of NOD1 with IC50 of 0.56 μM, inhibits NF-κB activation, exhibits 36-fold selectivity over NOD2.

Selleck's ML130 (Nodinitib-1) has been cited by 8 publications

2 Customer Reviews

  • (E) Gentamicin protection assay performed as in (B) with healthy control MDM (n=4). Quantification of S. typhimurium-CFU after inhibition of NOD1 with ML-130 and MDP pre-stimulation.

    Gut, 2016, 66(6):1060-1073. ML130 (Nodinitib-1) purchased from Selleck.

    Effect of ML130 on E. coli-induced NF- B activation in neutrophils. (A) Freshlyisolated neutrophils were pretreated with or without ML130 for 2 h, followed byexposure to live E. coli for 30 min. Nuclear translocation of NF- B was assessedby Western blot analysis. (B) The densitometric analysis of Western blots. Data(mean ± SEM of 4 heifers) are presented as fold changes over untreated samples afternormalisation to -actin within the same sample. One of 3 independent experimentsis shown. ** P < 0.01 and *** P < 0.001.

    Vet Immunol Immunopathol, 2015, 168(1-2):68-76. ML130 (Nodinitib-1) purchased from Selleck.

Purity & Quality Control

Choose Selective NOD Inhibitors

Biological Activity

Description ML130 (Nodinitib-1) is a potent and selective inhibitor of NOD1 with IC50 of 0.56 μM, inhibits NF-κB activation, exhibits 36-fold selectivity over NOD2.
NOD1 [1]
0.56 μM
In vitro

ML130 has shown selective inhibition of NOD1-induced NF-κB activation in HEK293 cells with no cytotoxicity and is selected as a probe candidate molecule. ML130 is also confirmed in secondary assays by selectively inhibiting NOD1-dependent IL-8 secretion and also selectively inhibiting the NOD1-dependent pathway to NF-κB activation. [1] In another research, ML130 is proved to cause conformational changes of NOD1 in vitro and alter NOD1 subcellular targeting in cells, providing chemical probes for interrogating mechanisms regulating NOD1 activity and tools for exploring the roles of NOD1 in various infectious and inflammatory diseases. [2]

Methods Test Index PMID
Western blot
p-NF-κB / NF-κB / TNFα; 

PubMed: 22450316     

HAPI cells were treated with ML130 (a Nod1 inhibitor) for 14 hour, followed by LPS. HAPI cells were collected, and pNF-κB was examined using Western blots. ML130 reduced the expression of pNF-κB (C). In the same sample, the expression of TNFα (D) was suppressed by ML130. *p<0.05, **p<0.01 vs vehicle.



Kinase Assay:[1]
- Collapse

NOD1 Dose Response assay:

Day 1 Procedure 1) Harvest HEK-293-T NFKB-Luc at 100% confluency at 100% confluency. 2) Add NOD assay media with Multidrop. 3) Spin down plates at 1000 rpm for 1 min in centrifuge. 4) Serial compound dilutions. 5) Add gamma-tri-DAP to cell suspension at 0.75 ug/mL. 6) Seed 13000 cells/well in 4 uL/well to full plate HEK-293-T NFKB-Luc to TC-treated plate. 7) Spin down plates 500 RPM for 5 min on centrifuge. 8) Lid Plates. Sandwich 4 plates between 2 lidded 384 plates filled with H2O. 9) Wrap plates securely in single layer of Plastic Wrap. 10) Incubate overnight (14 hours) in 37 ℃ and 5% CO2 incubator. Day 2 Procedure 1) Add 3 ul/well of SteadyGlo solution with Multidrop. 2) Shake plates on a plate shaker for 20 min. 3) Spin plates 1000 RPM for 1 min using centrifuge. 4) Read luminescence.IC50 values are calculated using GraphPad Prism 5.0.The average Z' for the screen is 0.6, the signal to background is 11.1, signal to noise is 78.6 and signal to window is 6.0.
Cell Research:[1]
- Collapse
  • Cell lines: Fa2N-4 cells
  • Concentrations: 0.01 μM-50 μM
  • Incubation Time: 24 h
  • Method: Hepatic toxicity of compounds is determined with Fa2N-4 immortalized human hepatocytes using the ATP-lite 1-step assay. Fa2N-4 cells are seeded at 50,000 cells/well, and incubated with a range of concentrations of the test compound (0.01 µM-50 µM) in MFE support medium for 24 h at 37 ℃, 5% CO2. At the end of the experiment D-luciferin and luciferase are added. The emitted luminescent signal produced as a result of the reaction with cellular ATP is captured on the Infinite M200 plate reader. The concentration of each compound that killed 50% of the cells (LC50) is calculated by non-linear regression analysis using a log (inhibitior) vs response equation with a variable slope, using the statistic software package Prism4.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (198.37 mM)
Water Insoluble
Ethanol '2 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 287.34


CAS No. 799264-47-4
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CC=C(C=C1)S(=O)(=O)N2C3=CC=CC=C3N=C2N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

NOD Signaling Pathway Map

Tags: buy ML130 (Nodinitib-1) | ML130 (Nodinitib-1) supplier | purchase ML130 (Nodinitib-1) | ML130 (Nodinitib-1) cost | ML130 (Nodinitib-1) manufacturer | order ML130 (Nodinitib-1) | ML130 (Nodinitib-1) distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID