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MBD2 + MBD3 Mouse mAb

Catalog No.: F3145

Application: Reactivity: Human

Usage Information

Dilution
WB1:500 IHC1:300

Application
WB, IHC, IF, FCM

Reactivity
Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
45 kDa, 33 kDa 45 kDa, 33 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
MBD2 + MBD3 Mouse mAb detects endogenous levels of total MBD2 + MBD3 protein.

Clone
F1H19

Synonym(s)
Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2, DMTase, MBD2

Background
MBD2 + MBD3 are closely related members of the methyl-CpG-binding domain (MBD) protein family, which function as epigenetic readers that influence chromatin structure and gene expression. Both proteins are expressed in various tissues, with MBD3 being essential for early embryonic development, while MBD2 is dispensable but influences processes such as maternal behavior. Structurally, they share a conserved MBD and a coiled-coil domain, but MBD2 uniquely contains an N-terminal glycine-arginine-rich region and a transcriptional repression domain (TRD), enabling it to bind methylated DNA and recruit the Mi-2/NuRD complex to silence transcription. In contrast, MBD3 lacks key residues in the MBD necessary for methylated DNA binding but remains a core component of the NuRD complex, where it regulates chromatin remodeling and differentiation. Despite their high sequence similarity and shared association with NuRD, MBD2 and MBD3 perform distinct, non-redundant roles in development and gene regulation, with MBD2 acting more directly in methylation-dependent repression and MBD3 playing a central role in lineage commitment and pluripotency control.

Background

MBD2 + MBD3 are closely related members of the methyl-CpG-binding domain (MBD) protein family, which function as epigenetic readers that influence chromatin structure and gene expression. Both proteins are expressed in various tissues, with MBD3 being essential for early embryonic development, while MBD2 is dispensable but influences processes such as maternal behavior. Structurally, they share a conserved MBD and a coiled-coil domain, but MBD2 uniquely contains an N-terminal glycine-arginine-rich region and a transcriptional repression domain (TRD), enabling it to bind methylated DNA and recruit the Mi-2/NuRD complex to silence transcription. In contrast, MBD3 lacks key residues in the MBD necessary for methylated DNA binding but remains a core component of the NuRD complex, where it regulates chromatin remodeling and differentiation. Despite their high sequence similarity and shared association with NuRD, MBD2 and MBD3 perform distinct, non-redundant roles in development and gene regulation, with MBD2 acting more directly in methylation-dependent repression and MBD3 playing a central role in lineage commitment and pluripotency control.

References
https://pubmed.ncbi.nlm.nih.gov/11274056/ https://pubmed.ncbi.nlm.nih.gov/25538734/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%