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Mast Cell Tryptase Mouse mAb

Catalog No.: F1781

Application: Reactivity: Dog, Monkey, Cat, Rat

Usage Information

Dilution
IHC1:10,000

Application
WB, IHC, IF, ELISA

Reactivity
Dog, Monkey, Cat, Rat

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

Biological Description

Specificity
Mast Cell Tryptase Mouse mAb recognizes endogenous levels of total Mast Cell Tryptase protein.

Clone
L14M18

Synonym(s)
TPS1, TPS2, TPSB1

Background
Mast cell tryptase is a highly conserved, tetrameric serine protease predominantly produced and stored in large quantities within the secretory granules of mast cells, which play a central role in allergic reactions, inflammation, and tissue defense. It exists mainly as a stable tetramer formed by four identical subunits (each ~30–36 kDa), with each monomer encompassing a classic serine protease catalytic triad (serine, histidine, aspartate), multiple cysteine-rich motifs forming disulfide bonds for structural stability, and distinct heparin-binding sites that stabilize the active enzyme and localize it to the acidic, heparin-rich granule core. The arrangement of tryptase tetramers, with active sites facing an internal cavity, protects the enzyme from endogenous inhibitors and regulates substrate specificity. Synthesized as an inactive proenzyme, tryptase is processed to its active form before being released extracellularly during mast cell degranulation, which occurs in response to allergic triggers or tissue injury. It is a pivotal mediator in both innate and adaptive immunity, cleaving extracellular matrix proteins to promote tissue remodelling. It activates protease-activated receptor 2 (PAR-2) on various cell types, thereby modulating vascular tone and neurogenic inflammation. Additionally, it stimulates cytokine secretion, such as IL-6 and TNF-α, which amplify inflammatory responses. Tryptase is critically involved in processes like bronchoconstriction, increased vascular permeability, leukocyte recruitment, smooth muscle proliferation, and angiogenesis. Elevated serum tryptase is a sensitive clinical biomarker of mast cell activation and is used in diagnosing and monitoring anaphylaxis, systemic mastocytosis, chronic urticaria, and related mast cell disorders; baseline or persistently high levels may indicate disease severity or progression.

Background

Mast cell tryptase is a highly conserved, tetrameric serine protease predominantly produced and stored in large quantities within the secretory granules of mast cells, which play a central role in allergic reactions, inflammation, and tissue defense. It exists mainly as a stable tetramer formed by four identical subunits (each ~30–36 kDa), with each monomer encompassing a classic serine protease catalytic triad (serine, histidine, aspartate), multiple cysteine-rich motifs forming disulfide bonds for structural stability, and distinct heparin-binding sites that stabilize the active enzyme and localize it to the acidic, heparin-rich granule core. The arrangement of tryptase tetramers, with active sites facing an internal cavity, protects the enzyme from endogenous inhibitors and regulates substrate specificity. Synthesized as an inactive proenzyme, tryptase is processed to its active form before being released extracellularly during mast cell degranulation, which occurs in response to allergic triggers or tissue injury. It is a pivotal mediator in both innate and adaptive immunity, cleaving extracellular matrix proteins to promote tissue remodelling. It activates protease-activated receptor 2 (PAR-2) on various cell types, thereby modulating vascular tone and neurogenic inflammation. Additionally, it stimulates cytokine secretion, such as IL-6 and TNF-α, which amplify inflammatory responses. Tryptase is critically involved in processes like bronchoconstriction, increased vascular permeability, leukocyte recruitment, smooth muscle proliferation, and angiogenesis. Elevated serum tryptase is a sensitive clinical biomarker of mast cell activation and is used in diagnosing and monitoring anaphylaxis, systemic mastocytosis, chronic urticaria, and related mast cell disorders; baseline or persistently high levels may indicate disease severity or progression.

References
https://pubmed.ncbi.nlm.nih.gov/15200544/ https://pubmed.ncbi.nlm.nih.gov/24793463/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%